• Genomics & Informatics
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• v.20 no.1
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• pp.11.1-11.20
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• 2022

Vibrio harveyi belongs to the Vibrio genus that causes vibriosis in marine and aquatic fish species through double-stranded DNA virus replication. In humans, around 12 Vibrio species can cause gastroenteritis (gastrointestinal illness). A large amount of virus particles can be found in the cytoplasm of infected cells, which may cause death. Despite these devastating complications, there is still no cure or vaccine for the virus. As a result, we used an immunoinformatics approach to develop a multi-epitope vaccine against most pathogenic hemolysin gene of V. harveyi. The immunodominant T- and B-cell epitopes were identified using the hemolysin protein. We developed a vaccine employing three possible epitopes: cytotoxic T-lymphocytes, helper T-lymphocytes, and linear B-lymphocyte epitopes, after thorough testing. The vaccine was developed to be antigenic, immunogenic, and non-allergenic, as well as having a better solubility. Molecular dynamics simulation revealed significant structural stiffness and binding stability. In addition, the immunological simulation generated by computer revealed that the vaccination might elicit immune reactions in the actual life after injection. Finally, using Escherichia coli K12 as a model, codon optimization yielded ideal GC content and a higher codon adaptation index value, which was then included in the cloning vector pET2+ (a). Altogether, our experiment implies that the proposed peptide vaccine might be a good option for vibriosis prophylaxis.

• Journal of Korean Association for Spatial Structures
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• v.22 no.3
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• pp.25-32
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• 2022

In this paper, the instability of the domed spatial truss structure using wood and the characteristics of the buckling critical load were studied. Hexagonal space truss was adopted as the model to be analyzed, and two boundary conditions were considered. In the first case, the deformation of the inclined member is only considered, and in the second case, the deformation of the horizontal member is also considered. The materials of the model adopted in this paper are steel and timbers, and the considered timbers are spruce, pine, and larch. Here, the inelastic properties of the material are not considered. The instability of the target structure was observed through non-linear incremental analysis, and the buckling critical load was calculated through the singularities and eigenvalues of the tangential stiffness matrix at each incremental step. From the analysis results, in the example of the boundary condition considering only the inclined member, the critical buckling load was lower when using timber than when using steel, and the critical buckling load was determined according to the modulus of elasticity of timber. In the case of boundary conditions considering the effect of the horizontal member, using a mixture of steel and timber case had a lower buckling critical load than the steel case. But, the result showed that it was more effective in structural stability than only timber was used.

• Journal of Electrochemical Science and Technology
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• v.12 no.2
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• pp.173-187
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• 2021

In the present research, a simple electrochemical sensor based on a carbon paste electrode (CPE) modified with ZnO-Zn₂SnO₄-SnO₂ and graphene (ZnO-Zn₂SnO₄-SnO₂/Gr/CPE) was developed for the direct, simultaneous and individual electrochemical measurement of Acetaminophen (AC), Caffeine (Caf) and Ascorbic acid (AA). The synthesized nano-materials were investigated using scanning electron microscopy, X-ray Diffraction, Fourier-transform infrared spectroscopy, and electrochemical impedance spectroscopy techniques. Cyclic voltammetry and differential pulse voltammetry were applied for electrochemical investigation ZnO-Zn₂SnO₄-SnO₂/Gr/CPE, and the impact of scan rate and the concentration of H⁺ on the electrode's responses were investigated. The voltammograms showed a linear relationship between the response of the electrode for individual oxidation of AA, AC and, Caf in the range of 0.021-120, 0.018-85.3, and 0.02-97.51 μM with the detection limit of 8.94, 6.66 and 7.09 nM (S/N = 3), respectively. Also, the amperometric technique was applied for the measuring of the target molecules in the range of 0.013-16, 0.008-12 and, 0.01-14 μM for AA, AC and, Caf with the detection limit of 6.28, 3.64 and 3.85 nM, respectively. Besides, the ZnO-Zn₂SnO₄-SnO₂/Gr/CPE shows an excellent selectivity, stability, repeatability, and reproducibility for the determination of AA, AC and, Caf. Finally, the proposed sensor was successfully used to show the amount of AA, AC and, Caf in urine, blood serum samples with recoveries ranging between 95.8% and 104.06%.

• Journal of IKEEE
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• v.26 no.4
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• pp.577-583
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• 2022

Verilog-HDL-based modeling can be performed to confirm the fast operation characteristics after setting the design parameters of each block considering the stability of the system by performing linear phase-domain modeling on the phase-locked loop. This paper proposed Verilog-HDL modeling including DCO noise and DTC nonlinear characteristic. After completing the modeling, the time-domain transient simulation can be performed to check the feasibility and the functionality of the proposed PLL system, then the phase noise result from the system design based on the functional model can be verified comparing with the ideal phase noise graph. As a result of the comparison of simulation time (6 us), the Verilog-HDL-based modeling method (1.43 second) showed 484 times faster than the analog transistor level design (692 second) implemented by TSMC 0.18-㎛.

• Analytical Science and Technology
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• v.36 no.4
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• pp.180-190
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• 2023

The presence of process related impurities in any drug or the drug product was associated with its safety, stability and efficacy. The overall literature survey proved that there is no method published on the assessment of process related impurities in brigatinib. In this study, a simple, reliable and stable HPLC qualitative method was reported for quantification of process related impurities with easy and quick extraction procedure. The impurities along with standard brigatinib was resolved on Lichrospher^® C18 (250 mm × 4.6 mm; 5 ㎛ particle size) column in room temperature using methanol, acetonitrile, pH 4.5 phosphate buffer in 55:25:20 (v/v) at 1.0 mL/min as mobile phase and UV detection at 261 nm. The method produces well resolved peaks at retention time of 4.60 min, 12.28 min, 3.37 min, 7.34 min and 8.39 min respectively for brigatinib, impurity A, B, C and D. The method produces a very sensitive detection limit of 0.0065 ㎍/mL, 0.0068 ㎍/mL, 0.0053 ㎍/mL and 0.0058 ㎍/mL for impurity A, B, C and D respectively with calibration curve linear in the concentration range of 22.5-135 ㎍/mL for brigatinib and 0.0225-0.135 ㎍/mL for impurities. The method produces all the validation parameters under the acceptable level and doesn't produces any considerable changes in peak area response while minor changes in the developed method conditions. The method can effectively resolve the unknown stress degradation products along with known impurities with less % degradation. The method can efficiently resolve and quantify the impurities in formulation and hence can suitable for the routine quality analysis of brigatinib in raw material and formulation.

• Proceedings of the Korean Vacuum Society Conference
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• 1998.02a
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• pp.17-17
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• 1998

Synchrotron radiation is produced when charged particles moving with relativistic velocities a are accelerated - for example, deflected by the bending magnets which guide the electron or p positrons in circular accelerators or storage rings. By using special focusing magnetic lattices i in the particle accelerators it is possible to make the dimensions of the particle beam very small with a hi맹 charge density which results in a light source with high b디lIiance. Synchrotron light h has important properties which make it ideal for a wide range of investigations in surface s science. The fact that the spectrum of electromagnetic radiation emitted in a bending magnet e extends in a continuum from the 얹r infra red region to hard x-rays means that it is id않I for a v variety of spectroscopic studies. Since there are no convenient lasers, or other really bright l light sources, in the vacuum ultraviolet and soft x-ray re.밍ons the development of synchrotron r radiation has enabled enormous advances to be made in this di펌C비t spectr따 re밍on. P Polarization-dependent measurements, for ex없nple ellipsometry or circular dichroism studies a are possible because the radiation has a well-defined polarization - linear in the plane of orbit w with additional right-circular, or left-circular, components for emission an생es above, or below, t the horizontal, respectively. Since the synchrotron light is emitted from a bunch of charge c circulating in a ring the light is emitted with a well-defined time structure with a short flash of l light every time a bunch passes an exit port. The time structure depends on the size of the ring a and the number and sequence of filling of the bunches. A pulsed light source enables time¬r resolved studies to be performed which provide direct information on the lifetimes and decay m modes of excited states and in addition opens up the possibility of using time of flight t techniques for spectroscopic studies. The fact that synchrotron radiation is produced in a clean u ultrahi야 vacuum environment is of gr않t importance for surce science studies. The current t비rd generation synchrotron light sources provide exceptionally high baliance and stability a and open up possibilities for experiments which would have been inconceivable only a short time ago.

• Journal of the Korean Geotechnical Society
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• v.25 no.9
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• pp.77-92
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• 2009

In the present paper, a numerical study was carried out for a reasonable and realistic evaluation of tension loads in piles during deep excavation in Singapore soft soil applying pile-plugged jet grouted slab. Based on 2-dimensional finite element analyses using linear elastic-perfectly plastic soil model obeying Mohr-Coulomb failure criterion, effects of pile-plugged jet grouted slab on the stability during excavation were examined, and a parametric study was also conducted to investigate critical influencing parameters in the estimation of reliable pile tension loads. Finally, based on the Modified Cam-Clay model, pile tension loads were estimated by considering on-going consolidation state of the Singapore clay deposit and the range of critical parameters observed during laboratory tests.

• Structural Engineering and Mechanics
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• v.86 no.4
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• pp.443-459
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• 2023

This study investigates the theoretical thermal buckling analyses of thick porous rectangular functionally graded (FG) plates with different geometrical boundary conditions resting on a Winkler-Pasternak elastic foundation using a new higher-order shear deformation theory (HSDT). This new theory has only four unknowns and involves indeterminate integral variables in which no shear correction factor is required. The variation of material properties across the plate's thickness is considered continuous and varied following a simple power law as a function of volume fractions of the constituents. The effect of porosity with two different types of distribution is also included. The current formulation considers the Von Karman nonlinearity, and the stability equations are developed using the virtual works principle. The thermal gradients are involved and assumed to change across the FG plate's thickness according to nonlinear, linear, and uniform distributions. The accuracy of the newly proposed theory has been validated by comparing the present results with the results obtained from the previously published theories. The effects of porosity, boundary conditions, foundation parameters, power index, plate aspect ratio, and side-to-thickness ratio on the critical buckling temperature are studied and discussed in detail.

• Journal of Electrochemical Science and Technology
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• v.14 no.2
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• pp.162-173
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• 2023

Aqueous zinc-ion batteries are considered as promising alternatives to lithium-ion batteries for energy storage owing to their safety and cost efficiency. However, their lifespan is limited by the irreversibility of Zn anodes because of Zn dendrite growth and side reactions such as the hydrogen evolution reaction and corrosion during cycling. Herein, we present a strategy to restrict direct contact between the Zn anode and aqueous electrolyte by fabricating a protective layer on the surface of Zn foil via phosphidation method. The Zn₃(PO₄)₂ protective layer effectively suppresses Zn dendrite growth and side reactions in aqueous electrolytes. The electrochemical properties of the Zn₃(PO₄)₂@Zn anode, such as the overpotential, linear polarization resistance, and hydrogen generation reaction, indicate that the protective layer can suppress interfacial corrosion and improve the electrochemical stability compared to that of bare Zn by preventing direct contact between the electrolyte and the active sites of Zn. Remarkably, MnO₂ Zn₃(PO₄)₂@Zn exhibited enhanced reversibility owing to the formation a stable porous layer, which effectively inhibited vertical dendrite growth by inducing the uniform plating of Zn²⁺ ions underneath the formed layer.

• Steel and Composite Structures
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• v.46 no.6
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• pp.839-856
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• 2023

This work presents a simple four-unknown refined integral plate theory for mechanical and thermal buckling behaviors of functionally graded (FG) plates resting on Visco-Pasternak foundations. The proposed refined high order shear deformation theory has a new displacement field which includes indeterminate integral variables and contains only four unknowns in which any shear correction factor not used, with even less than the conventional theory of first shear strain (FSDT). Governing equations are deduced from the principle of minimum total potential energy and a Navier type analytical solution is adopted for simply supported FG plates. The Visco-Pasternak foundations is considered by adding the impact of damping to the usual foundation model which characterized by the linear Winkler's modulus and Pasternak's foundation modulus. The accuracy of the present model is demonstrated by comparing the computed results with those available in the literature. Some numerical results are presented to show the impact of material index, elastic foundation type, and damping coefficient of the foundation, on the mechanical and thermal buckling behaviors of FG plates.