• Parasites, Hosts and Diseases
• /
• 제59권1호
• /
• pp.103-108
• /
• 2021

To date, there is no report on the genetic diversity of ticks in these regions. A total of 370 representative ticks from the south and east regions of Kazakhstan (SERK) and Xinjiang Uygur Autonomous Region (XUAR) were selected for molecular comparison. A fragment of the mitochondrial cytochrome c oxidase subunit I (cox1) gene, ranging from 631 bp to 889 bp, was used to analyze genetic diversity among these ticks. Phylogenetic analyses indicated 7 tick species including Hyalomma asiaticum, Hyalomma detritum, Hyalomma anatolicum, Dermacentor marginatus, Rhipicephalus sanguineus, Rhipicephalus turanicus and Haemaphysalis erinacei from the SERK clustered together with conspecific ticks from the XUAR. The network diagram of haplotypes showed that i) Hy. asiaticum from Almaty and Kyzylorda Oblasts together with that from Yuli County of XUAR constituted haplogroup H-2, and the lineage from Chimkent City of South Kazakhstan was newly evolved; and ii) the R. turanicus ticks sampled in Israel, Almaty, South Kazakhstan, Usu City, Ulugqat and Baicheng Counties of XUAR were derivated from an old lineage in Alataw City of XUAR. These findings indicate that: i) Hy. asiaticum, R. turanicus and Ha. erinacei shared genetic similarities between the SERK and XUAR; and ii) Hy. marginatum and D. reticulatus show differences in their evolution.

• Journal of Ginseng Research
• /
• 제46권1호
• /
• pp.104-114
• /
• 2022

Background: Abnormalities of myelin, which increases the efficiency of action potential conduction, are found in neurological disorders. Korean Red Ginseng (KRG) demonstrates therapeutic efficacy against some of these conditions, however effects on oligodendrocyte (OL)s are not well known. Here, we examined the effects of KRG-derived components on development and protection of OL-lineage cells. Methods: Primary OL precursor cell (OPC) cultures were prepared from neonatal mouse cortex. The protective efficacies of the KRG components were examined against inhibitors of mitochondrial respiratory chain activity. For in vivo function of Rb1 on myelination, after 10 days of oral gavage into adult male mice, forebrains were collected. OPC proliferation were assessed by BrdU incorporation, and differentiation and myelination were examined by qPCR, western blot and immunocytochemistry. Results: The non-saponin promoted OPC proliferation, while the saponin promoted differentiation. Both processes were mediated by AKT and extracellular regulated kinase (ERK) signaling. KRG extract, the saponin and non-saponin protected OPCs against oxidative stress, and both KRG extract and the saponin significantly increased the expression of the antioxidant enzyme. Among 11 major ginsenosides tested, Rb1 significantly increased OL membrane size in vitro. Moreover, Rb1 significantly increased myelin formation in adult mouse brain. Conclusion: All KRG components prevented OPC deaths under oxidative stress. While non-saponin promoted proliferation, saponin fraction increased differentiation and OL membrane size. Furthermore, among all the tested ginsenosides, Rb1 showed the biggest increase in the membrane size and significantly enhanced myelination in vivo. These results imply therapeutic potentials of KRG and Rb1 for myelin-related disorders.

• 한국노년학
• /
• 제34권2호
• /
• pp.277-297
• /
• 2014

본 연구는 청년기 손자녀의 인식을 기초로 조손간의 유대관계를 이해하는 것에 목적을 두었으며 손자녀 성(손자, 손녀)과 조부모 혈족(친조부모, 외조부모)에 따라 구분하여 조손관계를 분석하였다. 연구대상은 조부모님이 적어도 한 분 이상 생존해 계시는 만 19~25세의 청년세대 501명을 대상으로 자기보고식 설문지를 통해 평가하였다. 손자녀와 조부모간의 직접적 관계에 기초한 주요 특성변인들이 손자녀 성에 따라 차이가 있는지 t검증을 실시하고, 조손유대에 대한 부모특성변인들과 개인특성변인들의 설명력을 확인하기 위해 위계적 회귀분석을 실시하였으며, 그 결과는 다음과 같다. 첫째, 손자녀 성에 따라 친조부모와의 가치유사성, 친조부모와의 유대, 외조부모와의 접촉에서 유의미한 차이가 나타났다. 둘째, 지각된 아버지-조부모 관계 질, 어머니-조부모 관계 질, 접촉, 가치유사성은 모든 조손관계에서 조손유대를 유의미하게 예측하였으나, 각 특성변인의 상대적인 설명력은 각 조손관계에 따라 다양한 양상으로 나타나 조손유대를 설명하는 메커니즘이 손자, 손녀와 부계, 모계에 따라 다름을 보여주었다. 이러한 결과에 기초한 한국문화적 맥락에 따른 함의와 후속연구를 위한 제언을 논의에 제시하였다.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2022년도 추계학술대회
• /
• pp.257-257
• /
• 2022

Late blight, caused by the hemibiotrophic oomycete pathogen Phytophthora infestans, has been the most important disease limiting potato production worldwide. P. infestans undergo major population shifts in agricultural systems via the successive emergence and migration of asexual lineages. The phenotypic and genotypic bases of these selective sweeps are largely unknown but management strategies need to adapt to reflect the changing pathogen population. Here, we used molecular markers to divide the 86 South Korea isolates into six clonal lineages: KR_1_A1, KR_2_A2, SIB-1, US-11, SIB-1 like, and KR-2 like. We documented the emergence of a new lineage, termed SIB-1 like, and KR-2 like, and their rapid replacement of other lineages to exceed 35% of the pathogen population across South Korea. Genome analyses of the Korean P. infestans populations revealed extensive genetic polymorphism, particularly in effector genes. Importantly, SIB-1 like isolates carry an intact Avr8 effector gene that triggers resistance in potato carrying the corresponding R immune receptor gene R8 cloned from Solarium demissum. These findings point toward a strategy for deploying genetic resistance to mitigate the impact of the SIB-1 like lineage and illustrate how pathogen population monitoring, combined with genome analysis, informs the management of devastating disease epidemics. Further study is being done on pathogenicity of the SIB-1 like isolates on cultivated potatoes and changes in expression patterns of disease effector genes within the SIB-1 like isolates

• IMMUNE NETWORK
• /
• 제23권2호
• /
• pp.18.1-18.15
• /
• 2023

It has been reported that some exercise could enhance the anti-viral antibody titers after vaccination including influenza and coronavirus disease 2019 vaccines. We developed SAT-008, a novel digital device, consists of physical activities and activities related to the autonomic nervous system. We assessed the feasibility of SAT-008 to boost host immunity after an influenza vaccination by a randomized, open-label, and controlled study on adults administered influenza vaccines in the previous year. Among 32 participants, the SAT-008 showed a significant increase in the anti-influenza antibody titers assessed by hemagglutination-inhibition test against antigen subtype B Yamagata lineage after 4 wk of vaccination and subtype B Victoria lineage after 12 wk (p<0.05). There was no difference in the antibody titers against subtype "A." The SAT-008 also showed significant increase in the plasma cytokine levels of IL-10, IL-1β, and IL-6 at weeks 4 and 12 after the vaccination (p<0.05). A new approach using the digital device may boost host immunity against virus via vaccine adjuvant-like effects.

• Journal of Yeungnam Medical Science
• /
• 제14권2호
• /
• pp.359-369
• /
• 1997

저자들은 1993년 3월에서 1997년 9월까지 영남대학교 의과대학 부속병원 임상병리과에 면역학적 표지자 검사가 의뢰된 153명의 백혈병 환자의 결과를 분석하였다. 여기에는 AML 61례, ALL 46례, FAB 아형이 확정되지 않았던 12례 등이 포함되어 있었다. EDTA 항응고처리한 골수나 말초혈액에서 단핵세포층을 분리한후 일련의 단클론항체와 FITC 표지 이차항체를 이용하여 면역형광염색법을 실시한 후 형광현미경으로 관찰하였다. AML 61례에서의 비전형적인 표지자 표현 양상을 보면 CD7 32.8%, CD10 14.8%, CD5 13.1%, CD2 6.6%, CD4 4.9%, CD19 1.6%의 양성률을 보였고, TdT 양성은 3.9%였다. 혼성 백혈병으로 최종 진단을 내린 경우는 13.1%로 그 중 단구성 계열이 대부분(6/8)이었다. ALL 46례에서의 면역학적 검사에 따른 분류에 따르면 CALLA(+) B precursor형이 65.2%를 차지하였고, CALLA(-) B precursor형이 10.9%, T 세포형 8.7%, B 세포형 2.2%, 혼성림프구형(B&T) 4.3%, 미분화성 백혈병 2.2%였고, 혼성 백혈병으로 진단된 경우는 6.5%였다. 골수성 표지자 양성인 ALL은 CD13이나 CD33 양성인 례가 각각 2.2%였다. 급성 백혈병에서 골수형태와 세포화학검사로 FAB 아형을 확정하지 못한 12례에서 혼성 백혈병이 5례(41.7%)였고, 림프구 계열이 7례(58.3%)였으며 그 중 B 세포계열이 3례, T 세포계열이 3례, 혼성 림프구형(B&T)이 1례로 나타났다. 결론적으로는, 면역학적 표지지 검사로 더 정확하게 AML과 ALL을 감별할 수 있었으며, M0, M7 아형진단에도 도움이 되며, 형태학적, 세포화학적검사로 정확히 진단할 수 없었던 급성 백혈병의 분류에 도움이 되었다. 따라서 급성 백혈병의 진단을 위하여 FAB 분류 뿐 만 아니라 면역학적 표지자 검사를 함께 시행한다면 보다 정확한 진단에 도움을 줄 수 있을 것으로 생각된다.

• 대순사상논총
• /
• 제22권
• /
• pp.1-32
• /
• 2014

This paper is searching the meaning of Gangseong(姜聖) which came from Gucheon Sangje(九天上帝, the Lord and Creator of the Great Origin of the Ninth Heaven) incarnated in the Gang(姜) family. To study the meaning of Gangseong(姜聖), this paper concentrate on Maitreya(彌勒尊佛) and the sentence about Wonsibanbon(原始返本) Sangje's incarnation in the Gang's family appeared on term Gangseong in the middle of divinity terms(九天應元雷聲普化天尊姜聖上帝). Furthermore, Sangje's surname Gangseong(姜姓) has relation with Maitreya and the sentence about Wonsibanbon. What Maitreya has relation with Gangseong(姜聖) is the legendary poem that Maitreya Statue became complete woman(六丈金佛 化爲全女) and what Wensibanbon has relation with Gangseong is the fact that family name Gang is primary surname all over the world's human beings. Wonsibanbon comprises correction of family lineage and theocracy(政敎一致) which came from tracing and reviving the beginning. As a primary family name, Sangje's incarnation family name Gang plays a leading edge role of religious activity of Sangje because primary family name Gang represents the one and only Truth(眞法) or the principle of Wensibanbon which means the union[unity] of religion and politics(政敎一致), and combining saintliness and heroism (聖雄兼備). According to the one and only Truth(眞法) like this, the first emergence and role of the completely "DoTongKunJa" (道通君子, the perfection of man with Dotong) who Sang-Je declared will build the union[unity] of religion and politics shows realization of advance-oriented salvation. Under the principle of Yangsan(兩山, twin mountain, 甑山, 鼎山, the three-story Maitreya Statue of Geumsan Temple) which implies emergence of the completely "DoTongKunJa"(道通君子) who will accomplish the union[unity] of religion and politics(政敎一致), combining saintliness and heroism(聖雄兼備) and advent of the one and only Truth(眞法出現). This could be possible by the almighty of Sangje who incarnated in Gang's family name according to the principle of Wensibanbon from the text Jeonkyung(典經) as shown. Adjusting family lineage (血統) means to straighten out the relation between ancestors and descendants, and which has close relation with the activity after Sangje's incarnation in Gang's family name according to the principle of Wensibanbon. As far as family lineage was concerned, there are three aspects of close relation between ancestors and descendants. First, general connection between ancestors and descendants, Second Dotong(道通)-related connection, Third after -death entrance(冥府)-related connection. Descendants should know a family history from moral laws of family relationships(天倫) by the Compatibility principle. As for second Dotong-related connection between ancestors and descendants, the fact descendants' Dotong can be accomplished by the act of ancestors' charity shows human beings world(人間界) where descendants live has close organic relation with divine world(神明界) where ancestors live. As for third after-death entrance-related connection between ancestors and descendants, after-death entrance are totally related with both Heavenly order(天道) of ancestors and Humane-order(人道) of descendants. At this point, we can see the relation between ancestors and descendants are very close and organic. As shown from the text Jeonkyung, Sangje as Gucheon Sangje(九天上帝, the Lord and Creator of the Great Origin of the Ninth Heaven) and Maitreya(彌勒尊佛) has descended to the earth adopting Gang's family name according to principle Wensibanbon. By Sangje's incarnation in Gang's family name, the legitimacy of the religion and the perfection of Do could be successed under the law of Yangsan(兩山). Furthermore, Sangje realize the one and only Truth by Adjusting family lineage and producing the completely "true man" through Cheonji Gongsa.

• 대순사상논총
• /
• 제48집
• /
• pp.305-335
• /
• 2024

7~8세기에 중국 불교는 가장 융성했고 주요한 종파들이 등장해 각기 다른 길을 갔다. 이러한 사실은 잇따라 저술된 여러 고승전과 그 체재의 성격 변화를 통해서도 드러났다. 6세기 초, 혜교(慧皎)는 중국에 불교가 전래된 이후의 역사를 담은 『고승전(高僧傳)』을 편찬했다. 이때 십과(十科)라는 체재를 마련했는데, 645년에 도선(道宣)은 이 십과를 기본틀로 삼아 『속고승전(續高僧傳)』을 편찬했다. 그러나 십과의 일부를 수정하고 보완함으로써 십과를 역사서에 걸맞은 체재로서 정형화했다. 『속고승전』 이후에는 십과를 변형한 체재를 갖춘 고승전들이 편찬되었다. 법장(法藏)은 『화엄경전기』(690년?)를 저술하면서 십과로 구성했으나, 과목들의 명칭과 의미가 화엄철학을 강조하는 쪽으로 크게 변했다. 이어 혜상(惠詳)은 『홍찬법화전(弘贊法華傳)』(706년?)을 편찬하면서 『고승전』과 『속고승전』의 십과를 바탕으로 새롭게 변형한 팔과(八科)를 갖추었고, 승상(僧詳)은 『화엄경전기』의 십과에 두 과목을 새로 추가한 십이과(十二科) 체재의 『법화전기(法華傳記)』(750년?)를 편찬했다. 두 고승전의 체재는 철학보다 신앙에 방점을 찍었다. 선종에서도 8세기 초부터 고승전이 잇달아 편찬되었다. 『전법보기(傳法寶紀)』(713년?), 『능가사자기(楞伽師資記)』(713년?), 『역대법보기(歷代法寶記)』(774년), 『보림전(寶林傳)』(801년) 등이 그것이다. 그런데 네 고승전의 체재는 이전과 전혀 달랐다. 어떠한 과목도 설정하지 않고, 스승에서 제자로 이어지는 계보를 세워 서술했다. 이는 선종이 경전에 의거하지 않고 수행자가 스스로 깨닫는 것을 요체로 하기 때문이다. 그리고 처음에는 중국의 조사들만 입전하다가 『보림전』에 이르러서는 붓다 및 가섭 이하 27명의 조사들을 계보에 포함해 역사화했다. 이 허구적인 계보는 선종이 번성하면서 종파적 우월성과 정통성을 확보할 필요에 따른 것이었다.

• Journal of Microbiology
• /
• 제35권4호
• /
• pp.253-258
• /
• 1997

The nuclear 18S ribosomal RNA genes of seven corticioid species were sequenced. These sequences were analyzed and compared with those of 24 other species of the order Aphyllophorales and phylogenetic trees were constructed using parsimonious methods. Phylogenetic analyses showed that two species among examined members of the Corticiaceae, Resinicium bicolor and Thanatephorus praticola, are located distantly from the remaining six species. The separation of R. bicolor seems to be kphylogenetically significant because it has very unique cystidia. The independent lineage of T. practicola suggests that it is also phylogenetically distinct because it has unusual features like the homobasidium producing secondary spores and the spetal ultrastructure of pore cap. Furthermore, Auriscalpium vulgare, Bondarzewia berkeleyi, and Heterobasidion annosum from different families of the Aphyllophorales proved to be closely related to the species of the Corticiaceae. They all have amyloid spores and grouped with Aleyrodiscus amorphus, which is a member of the Corticiaceae. The amyloidity of spores seems to be an improtant character throughout the order of the Aphyllophorales.

• Journal of Microbiology and Biotechnology
• /
• 제19권4호
• /
• pp.346-350
• /
• 2009

Although salt is known to influence the performance of nitrification significantly, it has not been well reported on how salt affects ammonia-oxidizing bacterial(AOB) community compositions and dynamics in wastewater treatment bioreactors. In this study, these questions were evaluated in a full-scale bioreactor treating saline wastewater. Clone library analysis for the ammonia monooxygenase subunit A gene revealed that AOB belonging to the Nitrosomonas europaea and the N. oligotropha lineages inhabited in the bioreactor. Terminal restriction fragment length polymorphism analysis for monthly samples demonstrated a fluctuation pattern among AOB populations, although AOB within the N. europaea lineage were dominant during the test period. Correlation analysis between patterns of terminal restriction fragments and environmental variables suggested that sodium, chloride, and sulfate were less important; rather, temperature was the most significant factor affecting the AOB community in the bioreactor.