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Improvement of Boar Semen Quality by Sperm Selection Using Magnetic Nano-particles (마그네틱 나노비드를 이용한 돼지 정자 품질의 향상)

  • Chung, KI-Hwa;Son, Jung-Ho
    • Journal of Life Science
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    • v.26 no.8
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    • pp.943-947
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    • 2016
  • The objective of this study was to see if fairly simple magnetic nano-particle treatment enhances boar semen qualities. Boar semen samples were prepared from the swine AI center and samples were divided by 4 different motility groups (1, >90%; 2. 80~90%; 3. 70~80%; 4. <70%) using computer assisted sperm analysis (CASA) evaluation. Boar semen was extended using BTS extender and same number of magnetic nano-particles as total number of spermatozoa in each sample was treated for 20 min and collected for 5 min at room temperature. Sperm qualities such as motility and viability were evaluated by the CASA before and after treatment. Sperm abnormality and degree of agglutination were also evaluated under the microscopic examination before and after treatment. There were significant changes (p<0.05) on sperm motility from all 4 different groups in the average of 7.11% after treatment. The enhancement of sperm motility changes was more clear in the groups of lower sperm motile groups (<70% and 70~80%; 19.12±1.08% and 5.67±0.71%, p<0.05). The sperm motility character in terms of curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP) and linearity (LIN, %) showed also similar pattern but motility enhancement wear more clear in below 70% motile group. Average sperm viability was increased to 4% by magnetic nano-particles (p<0.05). The percentage of sperm abnormality was also reduced significantly (p<0.05) to the range of 3.7~4.5% before after treatment. The degree of sperm agglutination was also reduced in lower motility groups by the magnetic nano-particle purification.

Identification of the Protein Function and Comparison of the Protein Expression Patterns of Wheat Addition Lines with Wild Rye Chromosomes (야생 호밀 염색체 첨가 밀 계통의 단백질 발현 양상 비교 분석)

  • Lee, Dae Han;Cho, Kun;Woo, Sun Hee;Cho, Seong-Woo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.64 no.4
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    • pp.373-383
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    • 2019
  • The objectives of this study were to compare the protein expression patterns and degrees and identify the protein function of disomic addition lines (DAs) in Leymus racemosus, in order to improve the quality of wheat. Upon SDS-PAGE, L. racemosus showed two major protein bands whereas Chinese Spring (CS) had four major protein bands of high molecular weight. The DA(s) generally showed a similar protein expression pattern to that of CS, because 42 chromosomes were from CS and two chromosomes were from L. racemosus. However, only the L.r[J] line showed two protein bands of between 15 and 20 kDa, like L. racemosus. Image analysis based on 2-DE revealed that L.r[F] had the most upregulated protein spots, whereas L.r[N] had the least upregulated protein spots. For L.r[I], the frequency of the downregulated protein spots was higher than that of the upregulated ones. Using MALDI-TOF MS, the protein function was identified for each protein spot on the 2-DE polyacrylamide gel. The protein spots were classified into 11 groups according to protein function. Among the 11 groups, most protein spots of the DA(s) were identified as proteins related to metabolism. Additionally, unique protein spots of the DA(s) were related to abiotic stressors such as cold and heat. Those proteins are useful for improving wheat quality with resistance against abiotic stressors.

A Study on the Interpretalion of the Synthetic Unit Hydrograph According to the Characteristics of catchment Area and Runoff Routing (유역 특성과 유출추적에 의한 단위도 해석에 관한 고찰)

  • 서승덕
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.8 no.1
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    • pp.1088-1096
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    • 1966
  • The following is a method of synthetic unitgraph derivation based on the routing of a time area diagram through channel storage, studied by Clark-Jonstone and Laurenson. Unithy drograph (or unitgraph) is the hydrograph that would result from unit rainfall\ulcorner excess occuring uniformly with respect to both time and area over a catchment in unit time. By thus standarzing rainfall characteristics and ignoring loss, the unitgraph represents only the effects of catchment characteristics on the time distribution of runoff from a catchment The situation abten arises where it is desirable to derive a unitgraph for the design of dams, large bridge, and flood mitigation works such as levees, floodways and other flood control structures, and are also used in flood forecasting, and the necessary hydrologie records are not available. In such cases, if time and funds permit, it may be desirable to install the necessary raingauges, pruviometers, and stream gaging stations, and collect the necessary data over a period of years. On the otherhand, this procedure may be found either uneconomic or impossible on the grounds of time required, and it then becomes necessary to synthesise a unitgraph from a knowledge of the physical charcteristics of the catchment. In the preparing the approach to the solution of the problem we must select a number of catchment characteristic(shape, stream pattern, surface slope, and stream slope, etc.), a number of parameters that will define the magnitude and shape of the unit graph (e.g. peak discharge, time to peak, and base length, etc.), evaluate the catch-ment characteristics and unitgraph parameters selected, for a number of catchments having adequate rainfall and stream data and obtain Correlations between the two classes of data, and assume the relationships derived in just above question apply to other, ungaged, Catchments in the same region and, knowing the physical characteritics of these catchments, substitute for them in the relation\ulcorner ships to determine the corresponding unitgraph parameters. This method described in this note, based on the routing of a time area diagram through channel storage, appears to provide a logical line of research and they allow a readier correlation of unitgraph parameters with catchment characteristics. The main disadvantage of this method appears to be the error in routing all elements of rainfall excess through the same amount of storage. evertheless, it should be noted that the synthetic unitgraph method is more accurate than the rational method since it takes account of the shape and tophography of the catchment, channel storage, and temporal variation of rainfall excess, all of which are neglected in rational method.

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New data on Phase Relations in the System Cu-Fe-Sn-S (4성분계 Cu-Fe-Sn-S의 상관관계에 대한 새로운 데이터)

  • Jang, Young-Nam;Moh, Guenter
    • Journal of the Mineralogical Society of Korea
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    • v.4 no.1
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    • pp.43-50
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    • 1991
  • Two solid solution-type phases has been experimentally found in the quaternary system Cu-Fe-Sn-S:$(Fe, Cu, Sn)_{1+x}$ and $Cu_{2-x}Fe_(1+x}SnS_4$. These solid solutions are stable around the CuS-FeS-SnS referecne plane in the composition tetrahedron. One is the sphalerite-type monosulfide solid solution which has a extensive stability range with varying degrees of sulfur/metal ratio 9.7-1.0/1.0. The other is tetrahedrite-type phase $Cu_{2-y)Fe_{1+y}SnS_4(y_{max}=0.4)$ which is stable along the $Cu_2FeSnS_4-FeS$ tie line, but shows no phase transformation in the subsolidus range and decomposes incongruently at the range of 835-862${\circ}C$, depending on the compositional variation. Particularly, the latter phase shows the characteristic superstructure reflections, indicating that it is a derivative of sphalerite structure. The stability field of these two sphalerite-type phases are defined on the basis of diffraction pattern and optical homogeneity of the synthetic materials at the temperature range of 700-400${\circ}C$.

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Development of X-ray Detector using Liquid Crystal with Front Light (전면광원(Front Light)을 적용한 액정 X선 검출기 개발)

  • Rho, Bong Gyu;Baek, Sam Hak;Kang, Seok Jun;Lee, Jong Mo;Bae, Byung Seong
    • Journal of the Korean Society of Radiology
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    • v.13 no.6
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    • pp.831-840
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    • 2019
  • The X-ray detector by liquid crystal with front light was proposed and verified by a X-ray image. The proposed detector utilizes the visible light instead of the electric signal by transistor. Therefore, it shows low noise and can be fabricated at low cost. The liquid crystal detector uses the orientation change of the liquid crystal molecule by conductivity change of the photoconductive layer. We can get the X-ray image from the transmitted light through the liquid crystal. The X-ray dose was calibrated from the measured transmittance of the visible light after comparison to the reference transmittance curve of the liquid crystal. The amorphous Se was used for photo con ducting layer and parylene was used for the liquid crystal alignment instead of the conventional alignment layer which needs high-temperature process over 200℃. The proposed X-ray detector can decrease the X-ray dose by high sensitivity which was verified by simulation. After the fabrication of the X-ray detector, the X-ray image was obtained as a function of the bias voltage to the liquid crystal. 10 lines/mm resolution was obtained from the line pattern and we will apply it to the 17inch diagonal liquid crystal X-ray detector with 3π retardation.

Isolation and identification of Cyuptosporidium from various animals in Korea (한국산 동물로부터 크립토스포리디움의 분리 및 동정 III. 닭으로부터 Cryptosporidium baileyi의 분리)

  • Lee, Jae-Gu;Seo, Yeong-Seok;Park, Bae-Geun
    • Parasites, Hosts and Diseases
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    • v.29 no.4
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    • pp.315-324
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    • 1991
  • Each of SPF chicken(Hi-Line strain, 2-day-old males) was inoculated with 2.5 or $5\times10^4$ oocysts by stomach tube. The oocyst was the medium type of Cryptosporidium previously isolated from Korean chicken origin, and passed in 2-day-old SPF chicken. The patterns of oocyst discharge were monitored daily, and in order to observe the ultrastructure of the developmental stages, the bursa of Fabricius of the chicken was examined by transmission electron microscopy (TEM) on the 12th day postinoculation. The prepatent period for 8 chicken was 5.9 days postinoculation on the average, and the patent period was 12.9 days. The number of oocysts discharged per day for the chicken was reached peak on day 12 postinoculation on the average. A large number of oocysts was found in fecal samples obtained from inoculated chicken on days 8~14 postinoculation. The ultrastructural feature of almost every developmental stage of the medium type from chicken was very similar to that of Cryptosporidium previously isolated from mammalia including human and birds except for the attachment site of C. tsuris to the mucus cell from mammalia, but dimension of the oocysts from fecal samples of the medium type was different from those of C. meleagridis and mammalia origin. The above results reveal that the medium type of Cryptosporidium of Korean chicken origin is identified as Cryptosporidium baileyi.

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Medical Achievements of Doctor-Lee, Seokgan and Interpretation of the first unveiled 「Daeyakbu」 (조선 중기 유의(儒醫) 이석간(李碩幹)의 가계와 의약사적 연구 - 새로 발견된 대약부(大藥賦)를 중심으로 -)

  • Oh, Jun-Ho;Park, Sang-Young;Ahn, Sang-Woo
    • The Journal of Korean Medical History
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    • v.26 no.1
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    • pp.87-96
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    • 2013
  • This study confirmed that a doctor named Lee, Seok-gan whose name has been widely known but whose real identity has remained unclear, was an active Confucian doctor in the 16th century. In addition, through the newly discovered "Daeyakbu" among his family line, writings, and relics that have been handed down in a family, this study looked into his medical philosophy and medicine culture. The author of "Ieseokgangyeongheombang"(Medical Book by Lee, Seok-gan(李石澗), Seok-gan is the same person as an active famous doctor Lee, Seok-gan(李碩幹, 1509-1574) in the 16th century. Such a fact can be confirmed through "Samuiilheombang", "Sauigyeongheombang" and the newly opened "Ieseokgangyeongheombang". Lee, Seok-gan was born in the 4th ruling year of king Jungjong (1509) and was active as a doctor until the 7th ruling year of king Seonjo(1547); his first name is Jungim with the pen name-Chodang, and he used a doctor name of 'Seokgan.' He was known as a divine doctor, and there have been left lots of anecdotes in relation with Lee, Seok-gan. Legend has it that Seokgan went to China to give treatment to the empress, and a heavenly peach pattern drinking cup and a house, which the emperor bestowed on Seokgan in return for his great services, still have remained up to the present. Usually, Seokgan interacted with Toegye Lee Hwang and his literary persons, and with his excellent medical skills, Seokgan once gave treatment to Toegye at the time of his death free of charge. His medical skills have been handed down in his family, and his descendant Lee, Ui-tae(around 1700) compiled a medical book titled "Gyeongheombangwhipyeon(經驗方彙編)". Out of Lee, Seok-gan's keepsakes which were donated to Sosu museums by his descendant family, 4 sorts of 'Gwabu'(writings of fruit trees) including "Daeyakbu" were discovered. It's rare to find a literary work left by a medical figure like this, so these discoveries have a deep meaning even from a medicine culture level. Particularly, "Daeyakbu" includes the typical "Uigukron". The "Uigukron", which develops its story by contrasting politics with medicine, has a unique writing style as one of the representative explanatory methods of scholars' position during the Joseon Dynasty; in addition, the distinctive feature of "Uigukron" is that it was created in the form of 'Gabu' other than a prose.

Analyzing Spread Rate of Samcheok Forest Fire Broken out in 2000 Using GIS (GIS 응용(應用)에 의한 2000년(年) 삼척(三陟) 산불의 확산속도(擴散速度) 분석(分析))

  • Lee, Byung-Doo;Chung, Joo-Sang;Kim, Hyung-Ho;Lee, Si-Young
    • Journal of Korean Society of Forest Science
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    • v.90 no.6
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    • pp.781-787
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    • 2001
  • The spread rate of forest fire was analyzed on Samcheok forest fire that broke out on April 7, 2000 in Kunduck-Myun, Samcheok-City, Kangwon-Province and lasted for about 9 days. The spatial database including topography, overstory species distribution, micro-climate, daily fire front lines for the area was built using GIS and the daily spread pattern was investigated to determine a multiple regression equation to estimate forest fire spread rate. The results of the investigation showed that, on the first day, the forest fire spreaded out extremely fast up to 12.3m/min at about 10 a.m. until noon. After that, the forest fire spread rate fluctuated and slowed down as low as below 1m/min and quenched on April 15. The daily area-based spread rate along the fire spread line got to the peak of about 5,700ha on April 11, of which spread rates were recorded as 2.84m/min in the first half and 1.10m/min in the second half. Also, it was found that slope aspect, wind velocity and % area distribution of Pinus densiflora are the major factors affecting the spread rate of forest fire in this area.

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New Miniature Phalaenopsis 'Snow Angel' Having White Flower with Pink Lip as Pot Flower (백색에 분홍립 소형의 분화용 팔레놉시스 'Snow Angel' 육성)

  • Joung, Hyang Young;Lee, Young Ran;Kim, Mi Seon;Lim, Jin Hee;Shin, Hak Ki;Rhee, Hae Kyung;Park, Sang Kun
    • FLOWER RESEARCH JOURNAL
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    • v.19 no.3
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    • pp.168-171
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    • 2011
  • 'Snow Angel' was developed by National Institute of Horticultural & Herbal Science in 2008. A cross was made between phalaenopsis '0361', a white and red lip small-medium variety, and Phalaenopsis 'S98PN1', a white and dark pink lip small-medium variety with pink net pattern. The seedling materials were cultivated to 83 seedling lines from 2000 to 2002. The white and pink lip small-phalaenopsis '99-0404-13' with flowers of round shape was selected by individual selection. The selected line was multiplicated and the growth characteristics were investigated for the first characteristic trial from 2003 to 2007. It was named as 'Wonkyo F2-18' in 2008 and investigated for the second characteristic trial of growth characteristics. The examination was executed for stability, uniformity, yearly reproducibility, and public fancy. It was selected as a high quality cultivar with excellent flower shape and flower color from the selection council of new cultivars for agricultural products, there after it was registered as 'Snow Angel' in 2008.

Spatial Estimation of Point Observed Environmental Variables: A Case Study for Producing Rainfall Acidity Map (점관측 환경 인자의 공간 추정 - 남한 지역의 강우 산도 분포도 작성)

  • 이규성
    • Korean Journal of Remote Sensing
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    • v.11 no.3
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    • pp.33-47
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    • 1995
  • The representation of point-observed environmental variables in Geographic Information Systems(GIS) has often been inadequate to meet the need of regional-scale ecological and environmental applications. To create a map of continuous surface that would represent more reliable spatial variations for these applications, I present three spatial estimation methods. Using a secondary variable of the proximity to coast line together with rainfall acidity data collected at the 63 acid rain monitoring stations in Korea, average rainfall acidity map was cteated using co-kriging. For comparison, two other commonly used interpolation methods (inverse distance weighting and kriging) were also applied to rainfall acidity data without reference to the secondary variable. These estimation methods were evaluated by both visual assessments of the output maps and the quantitative comparison of error measures that were obtained from cross validation. The co-kriging method produced a rainfall acidity map that showed noticeable improvement in repoducing the inherent spatial pattern as well as provided lower statistical error as compared to the methods using only the primary variable.