• The KIPS Transactions:PartB
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• v.14B no.3 s.113
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• pp.191-198
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• 2007

In this paper, we present a marker detection and recognition method from camera image for a disabled person to interact with a server system which can control appliance of surrounding environment. It converts the camera image to a binary image by using multi-threshold and extracts contours of objects in the binary image. After that, it approximates the contours to a list of line segments. It finds rectangular markers by using geometrical features which are extracted from the approximated line segments. It normalizes the shape of extracted markers into exact squares by using the warping technique. It extracts feature vectors from marker image by using principal component analysis and then recognizes the marker. The proposed marker recognition system is robust for light change by using multi-threshold. Also, it is robust for angular variation of camera by using warping technique and principal component analysis. Experimental results show that the proposed method achieves 100% recognition rate at maximum for 21 markers and execution speed of 12 frames/sec.

• Journal of Electrical Engineering and Technology
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• v.13 no.6
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• pp.2530-2544
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• 2018

In this paper, we propose an enhanced LiDAR-camera calibration method that extracts the marker plane from 3D point cloud information. In previous work, we estimated the straight line of each board to obtain the vertex. However, the errors in the point information in relation to the z axis were not considered. These errors are caused by the effects of user selection on the board border. Because of the nature of LiDAR, the point information is separated in the horizontal direction, causing the approximated model of the straight line to be erroneous. In the proposed work, we obtain each vertex by estimating a rectangle from a plane rather than obtaining a point from each straight line in order to obtain a vertex more precisely than the previous study. The advantage of using planes is that it is easier to select the area, and the most point information on the board is available. We demonstrated through experiments that the proposed method could be used to obtain more accurate results compared to the performance of the previous method.

• Journal of the Korea Society of Computer and Information
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• v.11 no.4 s.42
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• pp.97-107
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• 2006

In this paper, we present a method for the detection and recognition of rectangular markers from a camera image. It converts the camera image to a binary image and extracts contours of objects in the binary image. After that. it approximates the contours to a list of line segments. It finds rectangular markers by using geometrical features which are extracted from the approximated line segments. It normalizes the shape of extracted markers into exact squares by using the warping technique. It extracts feature vectors from marker image by using principal component analysis. It then calculates the distance between feature vector of input marker image and those of standard markers. Finally, it recognizes the marker by using minimum distance method. Experimental results show that the Proposed method achieves 98% recognition rate at maximum for 50 markers and execution speed of 11.1 frames/sec for images which contains eleven markers.

• Radiation Oncology Journal
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• v.22 no.1
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• pp.69-75
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• 2004

Purpose : A new fiduciary plate and orientation marker have been devised to assist the quality assurance (QA) procedures for port films in radiation therapy department. The plate is used in conjunction with the film/cassette combination during weekly QA procedures, at Seoul National University Hospital (SNUH), in order to verify treatment fields in high radiation therapy. Materials and Methods : A new fiduciary plate was fabricated using an acrylic plate, cerrobend, standard blocking tray and mercury. The acrylic plate had the dimension of $1{\times}25{\times}25$ cm, with two fiduciary markers. The plate was rigidly attached onto the standard blocking tray, thus making it easier to set the fiduciary plate to the center on the radiation field on the linear accelerator. The plate had two 2-mm vertical and horizontal lines, with the minor scales in 2-cm steps. The orientation marker was a small mercury filled disk, which was inserted into the plate. Results : The geometrical structure of the lines in the plate makes it easier to correlate two different images between the simulation and port films. The marker clearly indicated the orientation of the film, for example, the anterior, posterior, left, right and various oblique orientations, without the placement of a conventional orientation marker. Also, the new orientation marker could easily be applied to the simulator by placing the small orientation marker onto the image intensifier or in front of the film/cassette holder. Conclusions : The new fiduciary plate appears to be useful in verifying the treatment fields, and the new orientation marker makes the film orientation simple, which is expected to lower the block fabrication errors.

• Molecules and Cells
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• v.40 no.5
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• pp.346-354
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• 2017

Long interspersed nuclear element-1 (LINE-1) is a retrotransposon that contains a CpG island in its 5'-untranslated region. The CpG island of LINE-1 is often heavily methylated in normal somatic cells, which is associated with poor prognosis in various cancers. DNA methylation can differ between formalin-fixed paraffin-embedded (FFPE) and frozen tissues. Therefore, this study aimed to compare the LINE-1 methylation status between the two tissue-storage conditions in gastric cancer (GC) clinical samples and to evaluate whether LINE-1 can be used as an independent prognostic marker for each tissue-storage type. We analyzed four CpG sites of LINE-1 and examined the methylation levels at these sites in 25 FFPE and 41 frozen GC tissues by quantitative bisulfite pyrosequencing. The LINE-1 methylation status was significantly different between the FFPE and frozen GC tissues (p < 0.001). We further analyzed the clinicopathological features in the two groups separately. In the frozen GC tissues, LINE-1 was significantly hypomethylated in GC tissues compared to their corresponding normal gastric mucosa tissues (p < 0.001), and its methylation status was associated with gender, differentiation state, and lymphatic and venous invasion of GC. In the FFPE GC tissues, the methylation levels of LINE-1 differed according to tumor location and venous invasion of GC. In conclusion, LINE-1 can be used as a useful methylation marker for venous invasion in both FFPE and frozen tumor tissues of GC.

• The Korean Journal of Food And Nutrition
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• v.36 no.6
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• pp.436-444
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• 2023

To produce an intestinal immunomodulatory beverage containing Centella asiatica extract (CAE), three types of CAE-added beverage prototypes were prepared, and their immunomodulatory activities and marker compounds were analyzed. As a result of the cytotoxicity assessment, all the beverages did not show significant toxicity compared to the control group. Next, the immunomodulatory activities of the beverage prototype were evaluated using the inflammatory model of IL-1β-induced intestinal epithelial cell line. All the samples significantly reduced the production of IL-6, IL-8, and MCP-1 in a CAE concentration-dependent manner. In addition, CAE-added beverages inhibited NO, IL-6, and IL-12 production in LPS-induced RAW 264.7 cells. When the major triterpenoids, as marker compounds for the production of CAE-added beverages, were analyzed by HPLC-DAD, only asiaticoside was detected beyond the limit of quantification, while madecassoside, madecassic acid, and asiatic acid were not detected. The amounts of asiaticoside in CAE-added beverage prototypes were confirmed in No. 1 (19.39 ㎍/mL), 2 (19.25 ㎍/mL), and 3 (19.98 ㎍/mL). In conclusion, the results of this study suggested that CAE-added beverage prototypes induced immunomodulatory effects in the intestinal inflammatory cell line models and asiaticoside could be used as a marker compound for CAE-added beverage production.

• Journal of Apiculture
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• v.32 no.3
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• pp.147-154
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• 2017

Honeybees (Apis mellifera) are common pollinators and important insects studied in agriculture, ecology and basic research. Recently, RDA (Rural Development Administration) and YIRI (Yecheon-gun Industrial Insect Research Institute) have been breeding a triple crossbred honey bee named Jangwon, which have the ability to produce superior quality honey. In this study, we identified a single nucleotide polymorphism (SNP) marker in the genome of Jangwon honeybee, particularly, in the paternal line (D line). Initially, we performed Sequence-Based Genotyping (SBG) using the Illumina Hiseq 2500 in 5 honeybee inbred lines; A, C, D, E, and F; and obtained 1,029 SNPs. Seventeen SNPs for each inbred line were generated and selected after further filtering of the SNP dataset. The 17 SNP markers validated by performing TaqMan probe-based real-time PCR and genotyping analysis was conducted. Genotyping analysis of the 5 honeybee inbred lines and one hybrid line, $D{\times}F$, revealed that one set of SNP marker, AmD9, precisely discriminated the inbred line D from the others. Our results suggest that the identified SNP marker, AmD9, is successful in distinguishing the inbred honeybee lines D, and can be directly used for genotyping and breeding applications.

• International Journal of Naval Architecture and Ocean Engineering
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• v.6 no.2
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• pp.206-218
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• 2014

The wave attenuation by floating breakwaters in high amplitude waves, which can lead to wave overtopping and breaking, is examined by numerical simulations. The governing equations, the Navier-Stokes equations and the continuity equation, are calculated in a fixed Cartesian grid system. The body boundaries are defined by the line segment connecting the points where the grid line and body surface meet. No-slip and divergence free conditions are satisfied at the body boundary cell. The nonlinear waves near the moving body is defined using the modified marker-density method. To verify the present numerical method, vortex induced vibration on an elastically mounted cylinder and free roll decay are numerically simulated and the results are compared with those reported in the literature. Using the present numerical method, the wave attenuations by three kinds of floating breakwaters are simulated numerically in a regular wave to compare the performance.

• International Journal of Industrial Entomology and Biomaterials
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• v.7 no.1
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• pp.11-14
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• 2003

NF733xin, the near allele line was obtained by means of crossing and backcrossing the silkworm race T6, which contained fluoride resistance major gene, to race 733xin, which was highly susceptible to fluoride toxicity. Two hundred RAPD random primers were used in the RAPD analysis of these 3 strains. Two molecular markers, OPB-08850 and OPB-10917, were obtained. OPB-10917 was used to detect the backcross generations. It was found that all the fluoride resistant individuals in each backcross generation had the same special band. These results proved that this marker was reliable.

• The Transactions of the Korean Institute of Electrical Engineers P
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• v.53 no.1
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• pp.1-6
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• 2004

In this paper, we present the new method for joint aspect inspecting system. We use the image processing and laser maker for light source. We can find the matrial joint status through processing the line pattern which is made by laser maker. To get the line pattern, in first, we did the preprocess of threshold. If the shape of line had over two segments, then the joint status is abnormal. We show our system efficency by experiment on tire facility.