• BMB Reports
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• v.43 no.5
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• pp.369-374
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• 2010

The PC12 is the widely used cell line to study neuronal differentiation. We had extensively investigated the details of protein expression in differentiated PC12 cells by proteomic analysis. The cells were incubated at the presence of nerve growth factor. We had analyzed the expression changes in the differentiating PC12 cells by 2-dimensional electrophoresis and the identification of the proteins using MALDI-TOF MS. By comparing expression pattern in the time course, we identified the candidate genes which are associated with neuronal differentiation. Among these genes, we performed real-time PCR analysis to validate $Idh3{\alpha}$ expression by the time course. To identify the function of $Idh3{\alpha}$ in neuronal differentiation stage, the transfection of $Idh3{\alpha}$ to PC12 cells was performed. As a result, we proved that up-regulation of $Idh3{\alpha}$ causes reduction in neural differentiation of PC12 cells. Based on these data, we suggest that $Idh3{\alpha}$ plays a role to the neuronal differentiation.

• Molecular & Cellular Toxicology
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• v.1 no.3
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• pp.149-156
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• 2005

Di (n-ethylhexyl) phthalate (DEHP) is thought to mimic estrogens in their action, and are called endocrine disrupting chemicals. DEHP is used in numerous consumer products, especially those made of flexible polyvinyl chloride and have been reported to be weakly estrogenic. In this study, DEHP were tested for estrogenic properties in vitro models and with microarray analysis. First, the E-screen assay was used to measure the proliferation of DEHP in MCF-7 cells, a human breast cancer cell line. DEHP induced an increase in MCF-7 cell proliferation at concentration of $10^{-4}M$. Second, we carried out a microarray analysis of MCF-7 cells treated with DEHP using human c-DNA microarray including 401 endocrine system related genes. Of the genes analyzed, 60 genes were identified showing significant changes in gene expression resulting from DEHP. Especially, 4 genes were repressed and 4 genes were induced by DEHP compared to $17{\beta}-estradiol$. Among these genes, trefoil factor 3 (intestinal), breast cancer 1, early onset and CYP1B1 are involved in estrogen metabolism and regulation. Therefore it suggests that these genes may be associated with estrogenic effect of the DEHP on transcriptional level. The rationale is that, as gene expression is a sensitive endpoint, alterations of these genes may act as useful biomarkers to define more precisely the nature and level of exposure to kinds of phthalates.

• Journal of The Korean Astronomical Society
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• v.35 no.4
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• pp.209-220
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• 2002

In order to increase the completeness of the investigations of stellar abundances, we can use spectrum synthesis method, new atomic data and observation of stellar spectra with resolution comparable to solar spectral atlases. We made a brief review of main problems of these three ways. We present new results of abundance determinations in the atmospheres of four stars. The first is the implementation of new atomic data to well known Przybylski's star. We show that the number of spectral lines, which can be identificated in the spectrum of this star, can be significantly higher. The second example is the investigation of $\zeta$ Cyg. We found the abundances of 51 elements in the atmosphere of this mild barium star. The third example is halo star HD221170. Our preliminary abundance pattern consists of 42 elements. The heaviest elements in this pattern are U and Th. The last star is the spectroscopic binary HD153720. The number of elements investigated in the spectra of components of this star is not large, but the results show that the components are Am-stars.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.20 no.4
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• pp.333-341
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• 2008

A remote sensing technique to identify trajectories of breaking waves in the surf zone using oblique digital nearshore videos is proposed. The noise arising from white foam induced by wave breaking has hindered accurate remote sensing of wave properties in the surf zone. For this reason, this paper focuses on image processing to remove the noise and wave trajectory identification essential for wave property estimation. The nearshore video imagery sampled at 3 Hz are used, covering length scale(100 m). Original image sequences are processed through image frame differencing and directional low-pass image filtering to remove the noise characterized by high frequencies in the video imagery. The extraction of individual wave crest features is conducted using a Radon transform-based line detection algorithm in the processed cross-shore image timestacks having a two-dimensional space-time domain. The number of valid wave crest trajectories identified corresponds to about 2/3 of waves recorded by the in-situ sensors.

• Molecules and Cells
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• v.25 no.1
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• pp.20-29
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• 2008

Cytoplasmic male sterility (CMS), one of the most important traits in crop breeding, has been used for commercial seed production by $F_1$ hybrid cultivars of pepper (Capsicum annuum L.). To develop reliable molecular markers for allelic selection of the Restorer-of-fertility (Rf) gene, which is known to be a major determinant of pollen fertility restoration in peppers, a sequence of approximately 10 kb flanking an RAPD fragment closely linked to the Rf locus was obtained by genome walking. A homology search revealed that this sequence contained an LTR retrotransposon and a non-LTR LINE-like retrotransposon. Sequencing of this Rf-linked region to search for polymorphisms between a dominant and recessive allele revealed 98% nucleotide sequence identity between them. A third polymorphic haplotype of the Rf-linked sequence, which has 94-96% nucleotide sequence identity with the two previously isolated haplotypes, was identified among a large number of breeding lines. Utilizing polymorphic sequences in the haplotypes, PCR markers were developed for selection of particular haplotypes and used to examine the distribution of the haplotypes in diverse breeding lines, cultivars, and C. annuum germplasms. Surprisingly, the third haplotype was the predominant type in C. annuum germplasms, while its frequency in $F_1$ hybrid cultivars was relatively low. Meanwhile, analysis of breeding lines whose Rf allele genotypes and male-sterility phenotypes were already known revealed that the third haplotype was mainly present in exotic breeding lines that cause unstable male-sterility when combined with sterile cytoplasms.

• Tunnel and Underground Space
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• v.18 no.6
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• pp.465-479
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• 2008

For the quantitative identification of rock behavior in shallow tunnels, we recommend using the rock behavior index (RBI) by the analytic hierarchy process (AHP) and the Rock Engineering Systems (RES). AHP and RES can aid engineers in effectively determining complex and un-structured rock behavior utilizing a structured pair-wise comparison matrix and an interaction matrix, respectively. Rock behavior types are categorized as rock fall, cave-in, and plastic deformation. Seven parameters influencing rock behavior for shallow depth rock tunnel are determined: uniaxial compressive strength, rock quality designation (RQD), joint surface condition, stress, pound water, earthquake, and tunnel span. They are classified into rock mass intrinsic, rock mass extrinsic, and design parameters. An advantage of this procedure is its ability to obtain each parameter's weight. We applied the proposed method to the basic design of Seoul Metro Line O and quantified the rock behavior into RBI on rock fall, cave-in, and plastic deformation. The study results demonstrate that AHP and RES can give engineers quantitative information on rock behavior.

• Korean Journal of Remote Sensing
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• v.30 no.6
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• pp.817-827
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• 2014

In the satellite remote sensing, the operational environment of the satellite sensor causes image degradation during the image acquisition. The degradation results in noise and blurring which badly affect identification and extraction of useful information in image data. This study proposes a maximum a posteriori (MAP) estimation using Point-Jacobian iteration to restore a degraded image. The proposed method assumes a Gaussian additive noise and Markov random field of spatial continuity. The proposed method employs a neighbor window of spoke type which is composed of 8 line windows at the 8 directions, and a boundary adjacency measure of Mahalanobis square distance between center and neighbor pixels. For the evaluation of the proposed method, a pixel-wise classification was used for simulation data using various patterns similar to the structure exhibited in high resolution imagery and an unsupervised segmentation for the remotely-sensed image data of 1 mspatial resolution observed over the north area of Anyang in Korean peninsula. The experimental results imply that it can improve analytical accuracy in the application of remote sensing high resolution imagery.

• BMB Reports
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• v.33 no.2
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• pp.184-187
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• 2000

Prostaglandin $E_2$ ($PGE_2$) plays an important role in the regulation of various gastric functions, and the growth-inhibitory activities on tumor cells are studied in vitro and in vivo. Although the mechanisms have attracted many researchers in the past decade, the molecular mechanisms of cell cycle arrest, or induction of apoptosis by $PGE_2$, is unclear. We investigated the effects of $PGE_2$ on the growth of the human gastric carcinoma cell line SNU1 and genes that are regulated by $PGE_2$ and isolated them using differential display RT-PCR (DD RT-PCR). FACS analysis suggested that SNU1 cells were arrested at the G1 phase by $PGE_2$ treatment. This growth inhibitory effect was in a time- and dose-dependent manner. Treatment of SNU1 cells with $10\;{\mu}g/ml$ $PGE_2$, followed by DD RT-PCR analysis, revealed differently expressed bands patterns from the control. Among the differently expressed clones, we found an unidentified cDNA clone (HGP-27) overexpressed in $PGE_2$-treated cells. The full-length cDNA of HGP-27 was isolated using RACE, which consisted of a 30-nt 5'-noncoding region, a 891-nt ORF encoding the 296 amino acid protein, and a 738-nt 3'-noncoding region including a poly(a) signal. This gene was localized on the short arm of chromosome number 11. Using the Motif Finder program, a myb-DNA binding repeat signature was detected on the ORF region. The COOH-terminal half was shown to have similarity with the $NH_3$-terminal domain of thioredoxin (Trx). This relation between HGP-27 and Trx implied a potential role for HGP-27 in modulating the DNA binding function of a transcription factor, myb.

• Science of Emotion and Sensibility
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• v.6 no.2
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• pp.1-7
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• 2003

The objectives of this study is to develop an adaptable digital wall model whose color can be changed dynamically according to the identified emotional state of a user. This study addresses how to capture a specific user's emotion through the web and use it for modifying VR model mainly for color adaptation. This adaptation process of a VR model consists of three phases: 1) identification of the user's emotional state projected onto the list of emotional keywords 2) translation of those captured emotional keywords into a pertinent set of color coordinations, and finally, 3) automated color adaptation process for the given model. This process derives an on-line viewer's emotional state, which can be utilized to find a new color scheme reflecting the identified emotion.

• Journal of Korean Neurosurgical Society
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• v.29 no.11
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• pp.1538-1541
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• 2000

Apenetrating head injury by an arrow is extremely rare being only two cases reported in the literature. We report a case of 12-year-old boy who presented with an arrow lodged at the posterior fossa through nasal nostril. He was slightly drowsy, but neurologically intact except a sixth nerve palsy in the left eye. Brain CT showed no intracerebral hemorrhage. The arrow was entered through the right nostril and the tip was stucked in the left cere-bello-pontine angle through the sphenoid sinus. We removed the arrow without craniotomy since the tip was verified smooth and no cerebral arteries were in contact with the arrow. The patient recovered well without any significant complications. The sixth nerve palsy resolved completely after six months. The verification of the shape of the offending material, a retrograde removal of the arrow in the direction of its line of trajectory, and an identification of adjacent cerebral arteries seem to be important in the management of this type of injury.