• Title/Summary/Keyword: Light Sensitivity

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Short Wave Infrared Imaging for Auroral Physics and Aeronomy Studies

  • Trond S. Trondsen;John Meriwether;Craig Unick;Andrew Gerrard;Matthew Cooper;Devin Wyatt
    • Journal of Astronomy and Space Sciences
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    • v.41 no.2
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    • pp.121-138
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    • 2024
  • Advances in solar-terrestrial physics are generally linked to the development of innovative new sensor technologies, affording us ever better sensitivity, higher resolution, and broader spectral response. Recent advances in low-noise InGaAs sensor technology have enabled the realization of low-light-level scientific imaging within the short-wave infrared (SWIR) region of the electromagnetic spectrum. This paper describes a new and highly sensitive ultra-wide angle imager that offers an expansion of auroral and airglow imaging capabilities into the SWIR spectral range of 900-1,700 nm. The imager has already proven successful in large-area remote sensing of mesospheric temperatures and in providing intensity maps showing the propagation and dissipation of atmospheric gravity waves and ripples. The addition of an automated filter wheel expands the range of applications of an already versatile SWIR detector. Several potential applications are proposed herein, with an emphasis on auroral science. The combined data from this type of instrument and other existing instrumentation holds a strong potential to further enhance our understanding of the geospace environment.

Light-Ontology Classification for Efficient Object Detection using a Hierarchical Tree Structure (효과적인 객체 검출을 위한 계층적 트리 구조를 이용한 조명 온톨로지 분류)

  • Kang, Sung-Kwan;Lee, Jung-Hyun
    • Journal of Digital Convergence
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    • v.10 no.10
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    • pp.215-220
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    • 2012
  • This paper proposes a ontology of tree structure approach for adaptive object recognition in a situation-variant environment. In this paper, we introduce a new concept, ontology of tree structure ontology, for context sensitivity, as we found that many developed systems work in a context-invariant environment. Due to the effects of illumination on a supreme obstinate designing context-sensitive recognition system, we have focused on designing such a context-variant system using ontology of tree structure. Ontology can be defined as an explicit specification of conceptualization of a domain typically captured in an abstract model of how people think about things in the domain. People produce ontologies to understand and explain underlying principles and environmental factors. In this research, we have proposed context ontology, context modeling, context adaptation, and context categorization to design ontology of tree structure based on illumination criteria. After selecting the proper light-ontology domain, we benefit from selecting a set of actions that produces better performance on that domain. We have carried out extensive experiments on these concepts in the area of object recognition in a dynamic changing environment, and we have achieved enormous success, which will enable us to proceed on our basic concepts.

An Ultrastructural Study on the Parotid Acinar Cells of X-irradiated Rats (X-선 조사를 받은 흰쥐 귀밑샘 샘포의 미세구조)

  • Yang, Nam-Gil;Park, In-Kyu;Ahn, E-Tay;Ko, Jeong-Sik;Park, Kyung-Ho;Kim, Jin-Gook
    • Applied Microscopy
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    • v.24 no.1
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    • pp.28-40
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    • 1994
  • Xerostomia and xerophthalmia are delicate or serous side effects, occuring when the radiotherapy is administered to the head and neck cancer patient. It is known that the cause of the above side effect is radiosensitivity of serous cells. In this study, the ultrastructural features of the parotid glands of the X-irradiated rats were observed. Sprague-Dawley rats weighing 200-250g each were anesthetized with sodium thiopental, and placed on the Mitsubishi linear accelerator. Only the head and neck areas of animals were exposured at the distance of 80cm, within the area of $30X30cm$, in the depth of 1cm, with the speed of 200R/min. Total doses applied were 3,000R or 6,000R depending on the experimental groups. Animals were sacrificed on the 6th hour, 2nd day and 6th day after the irradiation. Parotid glands were fixed in the 2.5% glutaraldehyde-1.5% paraformaldehyde solution, and followed by refixation in the 1% osmium tetroxide solution. Dehydrated blocks were embedded in araldite mixture, and ultrathin sections were cut. Sections were contrasted with the solution of uranyl acetate and lead citrate, and observed with JEM 100 CX-II electron microscope. The results were as follows: 1. Normal parotid acinar cells are two types; the light and the dark acinar cells. The light acinar cell contains dense secretory granules, whereas dark acinar cells contains granules of medium density with some darker spots within them, or other cells contain granules of medium density with darker rims. 2. Six hours after the irradiation, many acinar cells were degenerated showing variable stages of cytolytic bodies, light bodies, or dense degenerations. Within the acinar cell, Golgi apparatus and granular endoplasmic reticula were most severely altered elements. Granules showed more contrasting densities and irregularities. 3. Two days after the irradiation, some cytolytic bodies, and focal lucent degeneration of cytoplasm, and fine granular alteration of cytoplasmic matrix were pronounced. But other elements including secretory granules are rather looked unlatered. 4. Six days after the irradiation, most severe alterations were seen. Many intracellular canaliculi (or secretion figures), quanta of cytoplasm containing secretion antecedants, severely irregular luminal border, and again contrasting density of secretory granules showing tigroid spots or dense rims were noted. And myoepithelial degenerations were observed not uncommonly. 5. Irregular densities of secretory granules were interpreted as abnormal components of protein or carbohydrate portion are synthesized or abnormally metabolized under severe X-irradiation. 6. Myoepithelial degeneration and related alteration of nerve endings, etc., were suggested as the other causes of xerostomia following X-irradiation. 7. It is requested that radiation doses should be arranged, considering in mind not only the sensitivity of acinar cells but also the myoepithelial and neural functions.

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Design of DOI Detector Module for PET through the Light Spread Distribution (빛 분포를 통한 양전자방출단층촬영기기의 반응 깊이 측정 검출기 모듈 개발)

  • Lee, Seung-Jae;Baek, Cheol-Ha
    • Journal of the Korean Society of Radiology
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    • v.12 no.5
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    • pp.637-643
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    • 2018
  • A depth of interaction(DOI) detector module using a block scintillator and a pixellated scintillator was designed, and layer discrimination ability was calculated using DETECT2000. The block scintillator was used to improve the sensitivity and the spatial resolution was improved by measuring the DOI. The DOI was measured by analyzing the signal characteristics of each channel of the changed distribution of light. The detector module was composed to the block scintillator in the top layer and the pixellated scintillator in the bottom layer, which changes the distribution of light generated from a scintillator interacting with a gamma ray. In the flood image, the top layer was able to acquire the image at the position similar to the position of the bottom layer because the bottom layer consist of the pixellated scintillator. By using the Anger algorithm, the 16 channel signal was reduced to 4 channels to facilitate the analysis of the signal characteristics. The layer discrimination was measured using a simple algorithm and the accuracy was about 84% for each layer. When this detector module is used in preclinical PET, the spatial resolution at the outside of the field of view can be improved by measuring the DOI.

Development of an Imaging-DOAS System for 2-D Remote Sensing of Atmospheric Gases (대기가스오염물질의 이차원 원격 모니터링을 위한 Imaging-DOAS 개발)

  • Lee, Han-Lim;Lee, Chul-Kyu;Jung, Jin-Sang;Park, Jeong-Eun;Kim, Young-Joon
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.2
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    • pp.150-157
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    • 2006
  • Spatially resolved remote identification and quantification of trace gases in the atmosphere is desirable in various fields of scientific research as well as in public security and industrial contexts. Environmental observations investigating causes, extent md consequences of air pollution are of fundamental interest. We present an Imaging-DOAS system, a ground based remote sensing instrument that allows spatially resolved mapping of atmospheric trace gases by a differential optical absorption spectroscopy(DOAS) with sun scattered light as the light source. A passive DOAS technique permits the identification and quantification of various gases, e.g., $NO_2,\;SO_2,\;and\;CH_2O$, from their differential absorption structures with high sensitivity. The Imaging-DOAS system consists of a scanning mirror, a focusing lens, a spectrometer, a 2-D CCD, ad the integral control software. An imaging spectrometer simultaneously acquires spectral information on the incident light in one spatial dimension(column) and sequentially scans the next spatial dimension with a motorized scanning mirror. The structure of the signal acquisition system is described in detail and the evaluation method is also briefly discussed. Applications of imaging of the $NO_2$ contents in the exhaust plumes from a power plant are presented.

Adaptable Center Detection of a Laser Line with a Normalization Approach using Hessian-matrix Eigenvalues

  • Xu, Guan;Sun, Lina;Li, Xiaotao;Su, Jian;Hao, Zhaobing;Lu, Xue
    • Journal of the Optical Society of Korea
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    • v.18 no.4
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    • pp.317-329
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    • 2014
  • In vision measurement systems based on structured light, the key point of detection precision is to determine accurately the central position of the projected laser line in the image. The purpose of this research is to extract laser line centers based on a decision function generated to distinguish the real centers from candidate points with a high recognition rate. First, preprocessing of an image adopting a difference image method is conducted to realize image segmentation of the laser line. Second, the feature points in an integral pixel level are selected as the initiating light line centers by the eigenvalues of the Hessian matrix. Third, according to the light intensity distribution of a laser line obeying a Gaussian distribution in transverse section and a constant distribution in longitudinal section, a normalized model of Hessian matrix eigenvalues for the candidate centers of the laser line is presented to balance reasonably the two eigenvalues that indicate the variation tendencies of the second-order partial derivatives of the Gaussian function and constant function, respectively. The proposed model integrates a Gaussian recognition function and a sinusoidal recognition function. The Gaussian recognition function estimates the characteristic that one eigenvalue approaches zero, and enhances the sensitivity of the decision function to that characteristic, which corresponds to the longitudinal direction of the laser line. The sinusoidal recognition function evaluates the feature that the other eigenvalue is negative with a large absolute value, making the decision function more sensitive to that feature, which is related to the transverse direction of the laser line. In the proposed model the decision function is weighted for higher values to the real centers synthetically, considering the properties in the longitudinal and transverse directions of the laser line. Moreover, this method provides a decision value from 0 to 1 for arbitrary candidate centers, which yields a normalized measure for different laser lines in different images. The normalized results of pixels close to 1 are determined to be the real centers by progressive scanning of the image columns. Finally, the zero point of a second-order Taylor expansion in the eigenvector's direction is employed to refine further the extraction results of the central points at the subpixel level. The experimental results show that the method based on this normalization model accurately extracts the coordinates of laser line centers and obtains a higher recognition rate in two group experiments.

Conditions for Stable light Production of Recombinant Escherichia coli Containing Lux Operon and Sensitivity to Toxic Chemicals (Lux operon을 함유한 유전자 재조합 Escherichia coli의 발광 안정화 조건 및 독성물질에 대한 민감성)

  • 배희경;이상민;정윤철;송방호;신평균
    • KSBB Journal
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    • v.17 no.6
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    • pp.571-576
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    • 2002
  • Recombinant E. coli DH5 ${\alpha}$/pSB311 was made by cloning the genes encoding bacterial luciferase and aldehyde substrate proteins from Photohabdus luminescense, to complement defects of Lumistox, which is normally used in bioassays to monitor toxic substances in water environmental systems. The conditions for stable light production by the recombinant strains were investigated with respect to cell growth stage, cell number, and buffer conditions. The optimum growth stage was a middle-exponential stage with an OD$_{660nm}$ value of 0.6-0.7. ADout 10$^{6}$-10$^{7}$ cells per test tube was optimum for stable light emission. The effect of buffer was not significant if an optimum viable cell number was maintained. The bioluminescence of the recombinant E. coli harboring the lux operon of Photohabdus luminescense was not affected by temperature, while the bioluminescence of Lumistox was temperature sensitive. The recombinant E. coli was more sensitive to heavy metals (Cd, Cu, Hg, Zn) than Lumistox, because it does not require high concentrations of NaCl in the buffer.

Antagonistic Relationships Between Development of Sporangiophores and Phototropism Depending on Monochromatic Light Wavelengths in Phycomyces blakesleeanus (털곰팡이(Phycomyces blakesleeanus)의 생장기간 중 파장별 단색광 조사에 따른 굴광성 반응과 포자낭병 발생간의 길항관계)

  • Lee, Bum-Kyu;Park, Ae-Ri;Kim, Hye-Jeong;Shin, Dong-Ho;Choi, Kwan-Sam
    • Korean Journal of Agricultural Science
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    • v.36 no.1
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    • pp.27-40
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    • 2009
  • Six strains of Phycomyces blakesleeanus (DEL, A56, C9, C47, C111, and G5) were cultured in continuous light condition for 6 days. The increase in fresh and dry weight after the culture period was similar in all strains. However, the increase in fresh weight was sustained throughout the culture period in all strains, whereas the increase in dry weight was prominent only during the first 3 days of culture. The development and phototropism of macrophores was observed after 3 days of culture, These results suggest that the development and phototropism is ascribed to the increase in turgor pressure in sporangiophores where water entered. A56 and G5 showed a higher sensitivity to light than C9 and C47, whereas C111 showed no photosensitivity. Antagonistic relations were observed between photosensitivity and development of microphores, suggesting that the development and phototropism of macrophores is regulated by a common recognition factor (photoreceptor).

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Monitoring of pH and dissolved oxygen in microorganism fermentation processes using a 24-well microplate (24-웰 마이크로 플레이트를 이용한 미생물 발효공정에서 pH 및 용존산소의 모니터링)

  • Kim, Sun-Yong;Kim, Chun-Kwang;Sohn, Ok-Jae;Rhee, Jong-Il
    • KSBB Journal
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    • v.24 no.2
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    • pp.207-211
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    • 2009
  • In this study, the sensing membranes for detection of pH and dissolved oxygen(DO) were prepared by immobilizing 6-aminofluorescein or ruthenium complex onto the sol-gel matrixes of GPTMS, MTMS, and TEOS and then recoated with the mixture of hydrophobic sol-gel and graphite for light insulation. The pH and DO sensing membranes recoated with the light insulation layer showed a higher sensitivity than those without light insulation layer. The sensing membranes were immobilized on the wells of 24-well microplate and used to monitor the fluorescence intensity for pH and DO in E.coli JM109 and P.pastoris X-33 fermentation processes. The change of the fluorescence intensity in the DO sensing membrane agreed with the growth patterns of microorganisms, that the membranes are valuable to monitor the DO in fermentation processes. In the case of pH monitoring, the fluorescence intensity has showed good correlation to the off-line pH data, that the pH membranes are valuable to monitor pH values in fermentations.

Optimal Ambient Illumination Study for Soft-Copy Ultrasound Images (소프트 카피 초음파 이미지를 보기 위한 최적의 주변광 조도 연구)

  • An, Hyun;Lee, Hyo-Yeong
    • Journal of the Korean Society of Radiology
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    • v.13 no.2
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    • pp.209-216
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    • 2019
  • The purpose of this study was to suggest the optimum ambient illumination level for proper visualization in image inspection and reading on CRT and LCD monitors used for ultrasound and reading. The evaluators were divided into 4 groups: 20 (Ultra-sonographer: 20 groups (4 groups: ultra-sonographer, 1-5 years, 5 ultra-sonographers, 6 to 10 years, 5 ultra-sonographers, 11 to 15 years, The subjects were 32 questions. The evaluation method was image evaluation of ultrasonic soft copy images for 30 seconds per 10, 25, 100Lux ambient illumination. The evaluation results were evaluated as 6 points (Normal = Definitely no lesion), 2 points = possibly not a lesion, 3 points = probably not a lesion, 4 points = possibly a lesion, 5 points = probably a lesion, 6 points = Definitely a lesion). In this study, the results of ROC analysis according to ambient light illumination reading softcopy images used for lesion detection of all ultrasound images showed the highest sensitivity, specificity, and AUC results at 10Lux. It was found that optimal use of 10Lux for ambient light illumination would provide optimal detection of lesions in ultrasound soft copy images. Based on the future research data, it will be presented as basic data for designing ambient light brightness of ultrasound imaging laboratory and reading room.