• Title/Summary/Keyword: Light Sectioning Method

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Object Width Measurement System Using Light Sectioning Method (광절단법을 이용한 물체 크기 측정 시스템)

  • Lee, Byeong-Ju;Kang, Hyun-Soo
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.18 no.3
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    • pp.697-705
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    • 2014
  • This paper presents a vision based object width measurement method and its application where the light sectioning method is employed. The target object for measurement is a tread, which is the most outside component of an automobile tire. The entire system applying the measurement method consists of two processes, i.e. a calibration process and a detection process. The calibration process is to identify the relationships between a camera plane and a laser plane, and to estimate a camera lens distortion parameters. As the process requires a test pattern, namely a jig, which is elaborately manufactured. In the detection process, first of all, the region that a laser light illuminates is extracted by applying an adaptive thresholding technique where the distribution of the pixel brightness is considered to decide the optimal threshold. Then, a thinning algorithm is applied to the region so that the ends and the shoulders of a tread are detected. Finally, the tread width and the shoulder width are computed using the homography and the distortion coefficients obtained by the calibration process.

Current Status of Automatic Serial Sections for 3D Electron Microscopy

  • Choi, Hyosun;Jung, Min Kyo;Mun, Ji Young
    • Applied Microscopy
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    • v.47 no.1
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    • pp.3-7
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    • 2017
  • The automatic equipment for three-dimensional electron microscopy (3DEM) can acquire serial sections of a large sample in a relatively short time, and is especially suitable for the connectomics, which is a field related to understanding the brain structure as a whole. As many results obtained through 3DEM using automatic serial sections have been published in the field of brain research, many researchers continue to apply this technique to various samples. We reviewed the equipment for automatic serial sectioning, the block preparation method, the limitations of 3DEM, and future directions.

Proposal and design of reflecting optical system to improve detection intensity in fluorescence confocal scanning microscopy (형광 공초점 주사 현미경의 측정 강도 향상을 위한 반사 광학계의 제안 및 설계)

  • 강동균;서정우;권대갑
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2002.05a
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    • pp.187-190
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    • 2002
  • Confocal microscopy is very popular technology in bio-medical inspection due to its ability to reject background signals and to measure very thin slide of thick specimens, which is called optical sectioning. But intensity of detected signal in fluorescence type confocal microscopy is so small that only 0.2% of emitted fluorescence light can be detected in the best case. In this paper, we proposed the reflecting optical system to improve the detection intensity and designed the optical system by optimal design method. At the end of the paper, we analyzed the characteristics of the proposed reflecting optical system.

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Development of Rail Profile Measurement System using High Speed Laser Image Processing (고속영상처리에 의한 레일형상측정시스템 개발)

  • Moon, Chul-Yi;Kim, Man-Chul
    • Proceedings of the KSR Conference
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    • 2006.11b
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    • pp.1320-1325
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    • 2006
  • Railway track supports and guides train and it directly affects ride quality and safety. To maintain track quality, regular maintenance activity is essential but it degrades track usage since maintenance activity occupies the track during the work. To achieve maximum track efficiency, track inspection should be performed at commercial running speed. In this paper, we designed and developed high speed rail profile measurement system using laser light sectioning method with line laser generator, digital camera and DSP based image processing system. The measurement system can supports rail profile measurement at the speed of 300 Km/h that can be applied to KTX.

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Micro-CT evaluation of internal adaptation in resin fillings with different dentin adhesives

  • Han, Seung-Hoon;Park, Sung-Ho
    • Restorative Dentistry and Endodontics
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    • v.39 no.1
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    • pp.24-31
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    • 2014
  • Objectives: The purpose of present study was to evaluate the internal adaptation of composite restorations using different adhesive systems. Materials and Methods: Typical class I cavities were prepared in 32 human third molars. The teeth were divided into the following four groups: 3-step etch-and-rinse, 2-step etch-and-rinse, 2-step self-etch and 1-step self-etch system were used. After the dentin adhesives were applied, composite resins were filled and light-cured in two layers. Then, silver nitrate solution was infiltrated, and all of the samples were scanned by micro-CT before and after thermo-mechanical load cycling. For each image, the length to which silver nitrate infiltrated, as a percentage of the whole pulpal floor length, was calculated (%SP). To evaluate the internal adaptation using conventional method, the samples were cut into 3 pieces by two sectioning at an interval of 1 mm in the middle of the cavity and they were dyed with Rhodamine-B. The cross sections of the specimens were examined by stereomicroscope. The lengths of the parts where actual leakage was shown were measured and calculated as a percentage of real leakage (%RP). The values for %SP and %RP were compared. Results: After thermo-mechanical loading, all specimens showed significantly increased %SP compared to before thermo-mechanical loading and 1-step self-etch system had the highest %SP (p < 0.05). There was a tendency for %SP and %RP to show similar microleakage percentage depending on its sectioning. Conclusions: After thermo-mechanical load cycling, there were differences in internal adaptation among the groups using different adhesive systems.

HISTOLOGIC CHANGE OF THE POSTERIOR ATTACHMENT IN ANTERIOR DISC DISPLACEMENT OF THE TEMPOROMANDIBULAR JOINT-A NEW MODEL OF INTERNAL DERANGEMENT IN RABBITS (실험가토의 악관절원판 변위시 후방부착조직의 변화)

  • Kim, Tae-Woo;Ko, Jea-Seung;Chang, Young-il
    • The korean journal of orthodontics
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    • v.23 no.4 s.43
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    • pp.503-527
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    • 1993
  • This paper describes a new method to create an animal model for TMJ internal derangement in the New Zealand white rabbits and the light and electron microscopical changes of posterior attachment of them. Twenty six rabbits(2.5-3.0kg), four normal and twenty two experimental, were used. The right disc of experimental animal was displaced anteriorly without sectioning the posterior attachment and tied to the zygomatic arch with nylon not to be reduced to the original position. The left TMJ was sham-operated to be compared with its right experimental one. Normal animals were sacrificed one day and eight weeks after experiment. Experimental animals were sacrificed one day, ten days, three weeks, five weeks and eight weeks after surgery respectively. They were fixed intravenously with $2\%$ glutaldehyde under general anesthesia and the samples of them were processed for light and electron microscopic examination. The purpose of this experiment is to make a suitable animal model of disc displacement without reduction for studying and understanding the cellular and morphologic events in posterior attachment of TMJ including early changes which were difficult to be observed in human TMJs. The results of this investigation suggest the following conclusions : 1. Authors induced anterior disc displacement surgically in rabbits with new method to examine histologic changes of posterior attachment. Tissue reactions of this model seem to be similar to those observed in human disc displacement. We think this animal model for anterior disc displacement may be used to explore and evaluate objectively the effects of many treatment modalities in disc displacements. 2. The animal disease model showed inflammation at early stage(one and ten days). At this stage there were mild-to-severe mononuclear inflammatory cell infiltration, numerous newly formed vessels, vessel dilatation and engormement and many fibroblasts. 3. At middle stage(three weeks), fibrosis occurred, where fibroblasts decreased in number, but their cytoplasm was profuse indicating high activity. Collagen fibers increased in number and the tissue looked more dense. 4. At late stage(five weeks and eight weeks) showed degenerative changes including perforation of posterior attachment, disintegration of collagen fiber bundles, degeneration of fibroblasts, metastatic ossification, and dystrophic calcification.

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