• 사회복지연구
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• 제42권2호
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• pp.275-302
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• 2011

본 연구의 목적은 이야기성에 주목한 생애사연구를 위해 남성노인의 생애사를 재구성하는 데에 있다. 이에 해당 생애사를 내러티브-생애사 인터뷰 분석방법에 따라 재구성한 결과, 이야기된 생애사는 '독자로서의 의무가 이끄는 삶으로 엮여 들어가 "독자 행세" 를 하지 않을 수 없었던 삶' 이라는 생애사적 관점에 따라 구성되고 있는 반면, 체험된 생애사는 '자기주도적인 삶의 의지' 를 관철시키는 삶을 살아온 것으로 구성되는 차이가 있음을 조절메커니즘에 의거해 도출하였다. 그리고 이러한 양 생애사 간 차이는 생애사적 자기평가와 생애사적 실재성 간에 괴리가 있음을 의미하는 것으로 해석하였다. 이러한 결과에 의거해, 이야기성에 주목한 생애사연구가 노인복지실천과 노인복지학 분야 생애사연구 방법에 함의하는 점을 논의하였다.

• 보험의학회지
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• 제17권
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• pp.103-107
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• 1998

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2000년도 제29회 국제학술심포지움 및 추계학술대회
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• pp.35-35
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• 2000

• Journal of Microbiology and Biotechnology
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• 제17권7호
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• pp.1177-1182
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• 2007

Bacillus cereus group bacteria share a significant degree of genetic similarity. Thus, to differentiate and identify the Bacillus cereus group efficiently, a multiplex PCR method using the gyrB and groEL genes as diagnostic markers is suggested for simultaneous detection. The assay yielded a 400 bp amplicon for the groEL gene from all the B. cereus group bacteria, and a 253 bp amplicon from B. anthracis, 475 bp amplicon from B. cereus, 299 bp amplicon from B. thuringiensis, and 604 bp amplicon from B. mycoides for the gyrB gene. No nonspecific amplicons were observed with the DNA from 29 other pathogenic bacteria. The specificity and sensitivity of the B. cereus group identification using this multiplex PCR assay were evaluated with different kinds of food samples. In conclusion, the proposed multiplex PCR is a reliable, simple, rapid, and efficient method for the simultaneous identification of B. cereus group bacteria from food samples in a single tube.

• Journal of Microbiology and Biotechnology
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• 제33권3호
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• pp.371-377
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• 2023

In this study, a pepA gene encoding glutamyl (aspartyl)-specific aminopeptidase (PepA; E.C. 3.4.11.7) was cloned from Tetragenococcus halophilus CY54. The translated PepA from T. halophilus CY54 showed very low similarities with PepAs from Lactobacillus and Lactococcus genera. The pepA from T. halophilus CY54 was overexpressed in E. coli BL21(DE3) using pET26b(+). The recombinant PepA was purified by using an Ni- NTA column. The size of the recombinant PepA was 39.13 kDa as determined by SDS-PAGE, while its optimum pH and temperature were pH 5.0 and 60℃, respectively. In addition, the PepA was completely inactivated by 1 mM EDTA, indicating its metallopeptidase nature. The Km and Vmax of the PepA were 0.98 ± 0.006 mM and 0.1 ± 0.002 mM/min, respectively, when Glu-pNA was used as the substrate. This is the first report on PepA from Tetragenococcus species.

• 대한간호학회지
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• 제21권3호
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• pp.418-435
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• 1991

The purposes of this study were to find out the effects of hospice care for the quality of life of the terminally - ill patients and to analyze the relationship between the effect of hospice care and the general characteristics of subjects. The subjects of the study were 32 terminally - ill patients hospitalized in the two general hospitals in Seoul, which have the hospice care unit. The data were collected using the questionnaire with interviews. They were done from Sept.28, 1989 to March 20, 1991. The tools used for the research were 17-item questionnaire regarding general characteristics, 42-item questionnaire about the quality of life (11- item for physical, 16 for psychosocial, and 15 for spiritual). The questionnaires were to measure the duality of life by means of the measure instruments of Betty R. Farewell(1989), Stein Kaasa(1988), Palm Pamela(1987), and Hwa-sook Choi(1987). 42 items were used after pre-test. In accordance with each purpose in this study, frequency and percentage were used on the general characteristics of subjects. ANOVA, t-test, and Pearson correlation were employed to evaluate the general characteristics of subjects and different level of quality of life before-and-after hospice care. The results of the study may be summarized as follws : 1. The effects of hospice care. Main Hypothesis : “The quality of life of the terminally - ill patients will be different from before-and-after hospice care” was supported(t＝6.82, df＝31, p＝ .000). Sub Hypothesis 1 : “The quality of life of the terminally - ill patients in the physical aspects will be different from before - and -after hospice care” was not supported(t＝0.07, df＝31, p＝ .946). Sub Hypothesis 2 : “The quality of life of the terminally - ill patients in the psychosocial aspects will be different from before-and-after hospice care” was supported (t＝4.69, df＝31, p＝ .000). Sub Hypothesis 3 : “The quality of life of the terminally - ill patients in the spiritual aspects will be different from before-and-after hospice care” was supported(t＝6.64, df＝31, p＝ .000). 2. Relationship between the general characteristics of subjects and the effects of hospice care. (1) The more the number of family, the higher the quality of life in the psychosocial aspects. (2) The higher the age of the patient, the lower the quality of life in the psychosocial aspects. (3) The high educational level of patients enjoy the high quality of life in general. (4) The high religious level of petients enjoy the higy Quality of life in the spiritual aspects. As a results of analysis above : 1. The most effective aspects of the hospice care to the terminally- ill patients was spiritual aspects. The next effective aspects was psychosocial area. 2. The least effective aspcets of the hospice care to the terminally- ill patients was physical aspects. Further study is needed to improve the quality of life in the physical aspects.

• Mycobiology
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• 제45권4호
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• pp.379-384
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• 2017

In mating of Lentinula edodes, dikaryotic strains generated from certain monokaryotic strains such as the B2 used in this study tend to show better quality of fruiting bodies regardless of the mated monokaryotic strains. Unlike B2, dikaryotic strains generated from B16 generally show low yields, with deformed or underdeveloped fruiting bodies. This indicates that the two nuclei in the cytoplasm do not contribute equally to the physiology of dikaryotic L. edodes, suggesting an expression bias in the allelic genes of the two nuclei. To understand the role of each nucleus in dikaryotic strains, we investigated single nucleotide polymorphisms (SNPs) in laccase genes of monokaryotic strains to reveal nuclear origin of the expressed mRNAs in dikaryotic strain. We performed reverse transcription PCR (RT-PCR) analysis using total RNAs extracted from dikaryotic strains (A5B2, A18B2, and A2B16) as well as from compatible monokaryotic strains (A5, A18, and B2 for A5B2 and A18B2; A2 and B16 for A2B16). RT-PCR results revealed that Lcc1, Lcc2, Lcc4, Lcc7, and Lcc10 were the mainly expressed laccase genes in the L. edodes genome. To determine the nuclear origin of these laccase genes, the genomic DNA sequences in monokaryotic strains were analyzed, thereby revealing five SNPs in Lcc4 and two in Lcc7. Subsequent sequence analysis of laccase mRNAs expressed in dikaryotic strains revealed that these were almost exclusively expressed from B2-originated nuclei in A5B2 and A18B2 whereas B16 nucleus did not contribute to laccase expression in A2B16 strain. This suggests that B2 nucleus dominates the expression of allelic genes, thereby governing the physiology of dikaryons.

• 가정과삶의질연구
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• 제3권1호
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• pp.107-125
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• 1985

This paper investigated adult's consciousness with a view to establishing basic data about set-up any concrete plan and provision for elderly life in hoe and society. In this paper, the study of concerned reference and social survey by questionnaire are used. The data is obtained from 326 espondents who are 30 to 4 years old in Seoul and Andong. As to consciousness to the provision for elderly life and situation of set-up any concrete plan, the x2(chi-square) is applied to the analysis of regional, socio-economical, demographic difference. The conclusion of this study are as follows; 1) Although the respondents show positive attitude toward consciousness to the provision for elderly. life, their behavior does not show any sing of translating it into reality. 2) The consciousness to the provision for elderly life varies according to regional, socio-economical, demographic variance. Those who are in their 30's more-educated, and high-income want to be more independent from their children and express strong desire to set-up provision for elderly life in advance. This is also true of those living in city. As a results, as adults(30-54) recognize the importance of provision for elderly life, so the support by the society or governmental policy for the solution of better-life for old people is important. however, mental , physical, economical preparation in family life according to the family life cycle can basic solution.

• Preventive Nutrition and Food Science
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• 제14권4호
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• pp.283-289
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• 2009

We evaluated total phenolics and antioxidative activities of water extracts from different parts of Cudrania tricuspidata (specifically, the leaves, stems, roots, and fruits). The antioxidative activities of these samples were determined using five methods, including 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical scavenging, reducing power, ferric reducing ability of plasma (FRAP), and $\beta$-carotene/linoleic acid system. The water extract of leaves exhibited the higher DPPH, ABTS radical scavenging activities, reducing power, and FRAP than water extract of stem, roots, and fruits. Inhibition values on linoleic oxidation of water extracts from leaves, stems, roots, and fruits were calculated as 45.98%, 33.03%, 39.73%, and 25.48% at 10 mg/mL, respectively. The water extract of C. tricuspidata leaves had the highest amount of toal phenolics (73.60$\pm$0.28 mg/g). High-performance liquid chromatography (HPLC) analysis showed that quercetin is the predominant phenolic compound in water extract of leaves. Thus, our study verified that the water extract of leaves has strong antioxidant activities which are correlated with its high level of phenolic compounds, particularly quercetin. This water extract of C. tricuspidata leaves can be used as an effective and safe source of antioxidants.

• 가족자원경영과 정책
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• 제19권2호
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• pp.51-74
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• 2015

This study aimed to identify differences in the level of work-life balance as well as the effect of independent variables, including family (sharing family work, support for working), organization (culture, support services and systems), and community variables (accessibility to and amount of work-life balance programs), on work-life balance in South Korea, Japan, and the U.K. For these purposes, data were collected from 311, 324, and 322 married, working women (from 30 to 50 years of age) from Korea, Japan, and the U.K., respectively. It was consistently shown that U.K. employees scored higher in work-life balance than Korean and Japanese employees. Compared with Japan and the U.K., Korean participants were significantly lower in terms of work-leisure balance and work-self-development balance. The regression analysis revealed that 'sharing family work with partner' was commonly important and a major factor in all three countries. A 'supportive organizational culture' predicted work-life balance for Korean and Japanese participants, while work-life balance programs had a powerful effect on work-life balance only for U.K. participants. In the case of community variables, there were no significant effects for U.K. participants with regard to work-life balance. In contrast, 'the amount of work-life balance programs offered' was shown to affect the work-life balance of Korean working women, while 'accessibility to the programs' was significantly influential in Japan. We interpret these results according to social, economic, political, and psychological factors.