• 한국동물번식학회:학술대회논문집
• /
• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
• /
• pp.96-96
• /
• 2003

Nek2 (NIMA-related protein) is a mammalian cell cycle-regulated kinase that involves in chromosome condensation and centrosome regulation and NuMA (nuclear mitotic apparatus protein) is involved in spindle assembly during a cell cycle. The cellular distribution and organization of the centrosomal components is completely unknown during fertilization and embryonic development. We examined distribution of two well-known centrosomal proteins, Nek2 and NuMA in mouse gametes and embryos to get an insight in the reorganization of centrosomal proteins during germ cell development and early fertilization. Spermatogenic cells, gametes, and embryos were analyzed with anti-Nek2 or -NuMA antibodies by immunological assay, RT-PCR, and overexpression through gene transfection. Mitotically or meiotically active spermatogenic cells were intensively stained with these antibodies in both centrosomes and cytoplasm, whereas the oocytes showed different staining patterns depending on the meiotic stages. During maturation, GV, GVBD, and MI stage were clearly stained with NuMA antibody in the nucleus or cytoplasm at MII. Also, Nek2 was detectable in cytoplasm as scattered spots or chromosome associated at MII. In early developmental embryo, NuMA was detected in nucleus of each blastomere, while Nek2 was detected in cytoplasm. In contrast to previously reported results, Nek2 and NuMA were detected in both decondensing head, and the centriole of demembranated and decondensed sperm or whole body of trypsin-treated sperm for Nek2. During meiotic progress in oocytes, transcripts levels were the highest in MI stage and then downregulated in MII. Also, it shows dramatically change in early developmental embryos, firstly, it was increased until 4 cell stage and reduced in 8 cell stage, and finally, transcript levels were upregulated until blastoscyst. This finding suggests that cnetrosomal component may play an important role in reorganizing of functional centrosome during fertilization process and subsequent development.

• 디지털융복합연구
• /
• 제15권1호
• /
• pp.85-96
• /
• 2017

본 연구의 목적은 기업수명주기와 경영자 보상의 관계를 분석하는데 있다. 이를 위해 기업수명주기에 따른 경영자 보상 수준을 살펴보고, 수명주기별 경영자보상의 성과보상민감도를 살펴보고자 한다. 이와 같은 연구목적을 수행하기 위해, 2003년부터 2014년까지 유가증권시장 및 코스닥시장의 상장기업을 이용하여 회귀분석을 수행하였다. 분석 결과, 경영자의 보상수준은 도입기에서 가장 낮으며, 성숙기까지 증가하여 성숙기에서 가장 높으며, 쇠퇴기에서 급격하게 감소하는 것으로 나타났다. 성숙기를 제외한 도입기, 성장기, 쇠퇴기에서 성과보상민감도는 회계성과보다 주가성과에 더 연동되는 것으로 나타났으며, 성숙기에서는 회계성과에 강력하게 연동되는 것으로 나타났다. 추가분석을 통해 성장률이 높은 집단에서 회계성과와 주가성과에 연동되는 것으로 나타나 수명주기 결과가 성장률에 의해 나타난 결과가 아님을 확인하였다. 대규모기업집단을 별도로 분석하여 수명주기별 성과보상민감도가 일반적인 결과와 확연하게 다르게 나타남을 확인하였다. 이러한 결과는 기업의 수명주기에 따라 경영자 보상이 영향을 받는 다는 결과를 나타낸다는 점에서 기존연구와 차별성을 갖으며 관련 연구에 보탬이 된다.

• 한국지역지리학회지
• /
• 제8권1호
• /
• pp.20-39
• /
• 2002

본 연구는 우리나라의 대표적인 테마파크인 에버랜드를 사례로 급격한 관광수요 및 환경 변화에 대응하는 과정에서의 관광목적지 수명주기별 특성을 분석하였다. 에버랜드의 수명주기 단계는 일반적 지표(이용객수와 매출액)와 보조 지표(이용객수 속성과 이용객 유형)를 기준으로, 농원에서 위락공원으로의 변화가 진행된 초기개발기(1976${\sim}$1980년대 중반까지), 위락공원적 성격의 강화와 함께 테마파크로의 변화가 시작된 성장기(1980년대 중반${\sim}$1990년대 초반), 테마파크의 기반을 다졌으나 자원의 진부화가 진행되어 복합 테마파크화로의 변화를 계획한 성숙기(1990년대 초반${\sim}$1990년대 중반), 복합테마파크화의 실천 및 테마리조트파트로의 도약을 지향하는 재활성기(1990년대 중반이후)로 구분된다. 에버랜드는 새로운 시설 및 이벤트의 도입 또는 개선을 통하여 끊임없이 혁신을 행함으로써 현재 시장권도 서울중심에서 경기, 인천, 충북, 대전 등 전국으로 확대되고, 외국방문객도 급증하고 있다.

• Animal Bioscience
• /
• 제34권4호
• /
• pp.575-583
• /
• 2021

Objective: The aim of the study was to determine the effect of parity and the stage of lactation on the metabolic profile of cows based on the basic chemical milk components and the blood parameters. Methods: The study material consisted of high-yielding Holstein-Friesian cows. In total, 473 cows were examined. According to the parity, cows were divided into four groups: primiparous (P), and multiparous in the second (M2), in the third (M3), and in subsequent lactations (M4). The feeding of cows was based on total mixed ration (TMR) ad libitum. Milk and blood samples were collected individually from each cow three times per standard lactation period. Results: Greater exacerbation of changes in the dynamics of the blood plasma parameters examined was proved for multiparous cows. The highest value of β-hydroxybutyrate acid (0.946 mmol/L) was found for multiparous cows from group M3 at the beginning of lactation. However, it was still in the normal range. The results showed aspartate aminotransferase, and gamma-glutamyl transferase (GGT) activities in dairy cows during lactation had significant variations taking in to account stage of lactation. The highest activity of GGT was found in the group of the oldest cows and measured from 26.36 U/L at the beginning of lactation to 48.75 U/L at the end of the lactation period. Conclusion: The time-related changes in the concentrations of the biochemical parameters described differ markedly among lactating cows, though the housing conditions on the research dairy farm are highly standardised. This indicates that the ability to cope with metabolic stress is mainly affected by the individual predispositions of cows and feed nutrient supply in different stage of lactation. Especially, the feed nutrient supply (in net energy for lactation), which was the best in TMR 1 in comparison TMR 3.

• Communications for Statistical Applications and Methods
• /
• 제26권2호
• /
• pp.163-173
• /
• 2019

Simon's two-stage designs are frequently used in phase II single-arm trials for efficacy studies. A concern of safety studies is too many patients who experience an adverse event. We show that Simon's two-stage designs for efficacy studies can be similarly used to design a two-stage safety study by modifying some of the design parameters. Given the type I and II error rates and the proportion of adverse events experienced in the first stage cohort, we prescribe a procedure whether to terminate the trial or proceed with a stage 2 trial by recruiting additional patients. We study the relationship between a two-stage design with a safety endpoint and an efficacy endpoint as well as use simulation studies to ascertain their properties. We provide a real-life application and a free R package gen2stage to facilitate direct use of two-stage designs in a safety study.

• 대한가정학회지
• /
• 제32권5호
• /
• pp.55-66
• /
• 1994

The aim of this study is to propose the reasonable method of evaluation on the value of the life-time household work. for homemaker claim her share in the matrimonial property in relation to her life-time household work and to provide the basic data to measuring proper compensation for homemaker and calculating of the donation tax and inheritance tax amount. Tom compare the difference of the method of evaluation on the value of the life-time household work is evaluted by three methods: the model of the life-time household work which is reflected major variables in the FLC frame work marital stage, total marital period. The major findings are as follows: 1) Among the three evaluation me？ The method of Total marital period is evaluatied the lowest value of the life-time household work. 2) The evaluation method of marital stage is more resonable method than the method of total marital period habe a convenience of evaluation but it dos'nt reflected major variables which influence the value of life-time household work. 3) the most resonable method of evaluation on the value of the life-time household work is revealed the model of the life-time household work which is reflected major variables in the FLC frame work the age of first and last offspring marital period.

• 한국주거학회논문집
• /
• 제27권1호
• /
• pp.1-11
• /
• 2016

Based on residents' survey data in Seoul, this study attempts to empirically answer to four research questions regarding neighborhood relations (NR) which are necessary for community building: How important NR is compared to other social relations, what factors establish NR, how large NR's geographical extent is, and what are major meeting places among neighbors. A series of statistical analyses demonstrates that characteristics of NR vary greatly by family life cycle. In the stage of couple without child, NR has no significant meaning. NR becomes important from the stage of child care, and it is formed through the medium of children, particularly children's schools in the stage of child of elementary school as well as secondary schools. As the result, NR's geographical boundary extends beyond residential block or multi-family housing (apartment) estate while cafe and restaurant serve as dominant meeting places in these life cycle stages. On the contrary, after children are married and leave home, casual encounters resulted from spatial proximity become an important cause of NR. Likewise neighbors' geographical distribution is relatively concentrated within residential block or apartment estate, while community facilities play a role of meeting places as originally planned and designed.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제10권2호
• /
• pp.101-106
• /
• 2005

In this study, we describe the Bombyx mandarina hemolin cDNA. A sequence analysis of cDNA revealed a single open reading frame (ORF) of 1233 nucleotides. The deduced 410 amino acid sequence of B. mandarina hemolin contains 4 imunoglobulin (Ig) C-2 type domains. B. mandarina hemolin cDNA showed the highest sequence homology to known those of B. mori. The developmental profile in terms of expression level of hemolin mRNA was determined in the absence of a bacterial challenge. Hemolin mRNA was detected only in mid-gut, but not in hemocytes, fat body, testis, and silkglands. Hemolin mRNA in mid-gut was not detected until the spinning stage of the last instar larva, however, lit dramatically increased at the beginning of spinning and gradually decreased until pupal stage.

• 한국환경교육학회지:환경교육
• /
• 제7권1호
• /
• pp.88-105
• /
• 1994

Since the Rio Convention of 1992, the international community has displayed a heightened consciousness concerning the Earth environment and domestic attention on the said subject has also rapidly increased. An Ecological life style signifies the conscious efforts to preserve the environment interpreted into quotidien action. For the realization of conservation in action, an understanding of the specific details of environmental preservation must precede. The environmental conservation action in the homes was systematically approached in this study as an input-process-output and feedback cycle. The input phage is the consumer oriented buying stage, the while the process is the actual use of the products and the output stage consists of categorizing and producing wales, the feodbach being recycling of resources. At each stage the attitude which is devoted to environmental consciousness plays a big ride in the actual preservation of the Earth environment. in order to effectively conserve the environment, efforts displayed by the business sector is as important as implementation of environmentally conscious action at home. During the process of producing and distributing products, and attitude and action in preservation efforts are crncial and the responsibility ageat. For the business sector to effectively initiate environmental preservation into action, institutional support from the government and cooperation from the homes is of essence. Consolidated efforts to atter the superfrony life style into ecological life styles and ralues is essential to solve the most important socio-economic issue of the 90's of preserving the earth.

• 한국간호교육학회지
• /
• 제21권1호
• /
• pp.65-74
• /
• 2015

Purpose: This study was carried outto compare dietary self-efficacy, obesity stress, and obesity-related quality of life (OQOL) according to BMI and stages of change in vegetable consumption. Methods: A convenience sample of 326 nursing students agreed to complete a questionnaire. Data were collected from October 7 to October 18, 2013. Results: Obesity stress and obesity-related quality of life were significantly different according to BMI. Dietary self-efficacy showed a significant difference between the pre-contemplation/contemplation stage, preparation stage, and action/maintenance stage (F=50.18, p<.001). With obesity stress, there was a significant difference between the PC/C, P, and A/M stages (F=17.63, p<.001). Dietary self-efficacy had a positive correlation with OQOL (r=.11, p<.001) and a negative correlation with obesity stress (r=-.14, p=.012). And obesity stress had a negative correlation with OQOL (r=-.45, p<.001). Conclusion: These findings emphasize that nutritional-intervention programs for changes in behavior during the PC/C and P stages of change in vegetable consumption need to develop strategies to enhance dietary self-efficacy for nursing students.