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The current state of phage therapy in livestock and companion animals

  • Youbin Choi;Woongji Lee;Joon-Gi Kwon;Anna Kang;Min-Jin Kwak;Ju-Young Eor;Younghoon Kim
    • Journal of Animal Science and Technology
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    • v.66 no.1
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    • pp.57-78
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    • 2024
  • In a global context, bacterial diseases caused by pathogenic bacteria have inflicted sustained damage on both humans and animals. Although antibiotics initially appeared to offer an easy treatment for most bacterial infections, the recent rise of multidrug-resistant bacteria, stemming from antibiotic misuse, has prompted regulatory measures to control antibiotic usage. Consequently, various alternatives to antibiotics are being explored, with a particular focus on bacteriophage (phage) therapy for treating bacterial diseases in animals. Animals are broadly categorized into livestock, closely associated with human dietary habits, and companion animals, which have attracted increasing attention. This study highlights phage therapy cases targeting prominent bacterial strains in various animals. In recent years, research on bacteriophages has gained considerable attention, suggesting a promising avenue for developing alternative substances to antibiotics, particularly crucial for addressing challenging bacterial diseases in the future.

Suppression of Rice Stripe Virus Replication in Laodelphax striatellus Using Vector Insect-Derived Double-Stranded RNAs

  • Fang, Ying;Choi, Jae Young;Park, Dong Hwan;Park, Min Gu;Kim, Jun Young;Wang, Minghui;Kim, Hyun Ji;Kim, Woo Jin;Je, Yeon Ho
    • The Plant Pathology Journal
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    • v.36 no.3
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    • pp.280-288
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    • 2020
  • RNA interference (RNAi) has attracted attention as a promising approach to control plant viruses in their insect vectors. In the present study, to suppress replication of the rice stripe virus (RSV) in its vector, Laodelphax striatellus, using RNAi, dsRNAs against L. striatellus genes that are strongly upregulated upon RSV infection were delivered through a rice leaf-mediated method. RNAi-based silencing of peroxiredoxin, cathepsin B, and cytochrome P450 resulted in significant down regulation of the NS3 gene of RSV, achieving a transcriptional reduction greater than 73.6% at a concentration of 100 ng/μl and, possibly compromising viral replication. L. striatellus genes might play crucial roles in the transmission of RSV; transcriptional silencing of these genes could suppress viral replication in L. striatellus. These results suggest effective RNAi-based approaches for controlling RSV and provide insight into RSV-L. striatellus interactions.

Effects of stress after road transportation and oral administration of chromium and meloxicam on plasma cortisol concentrations and behavior in dairy calves

  • Jung, Da Jin Sol;Lee, Jaesung;Kim, Do Hyun;Beak, Seok-Hyeon;Hong, Soo Jong;Jeong, In Hyuk;Yoo, Seon Pil;Lee, Jin Oh;Cho, In Gu;Fassah, Dilla Mareistia;Kim, Hyun Jin;Baik, Myunggi
    • Animal Bioscience
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    • v.35 no.3
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    • pp.503-510
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    • 2022
  • Objective: This study was performed to determine the effects of stress after road transportation and oral administration of chromium and meloxicam on growth performance, plasma cortisol, serum metabolites, and behavior in dairy calves. Methods: A total of 50 Holstein heifers (average body weight [BW]: 172±4.19 kg; average age: 5.53±0.12 months) were randomly assigned to five groups including NL (not transported + D-lactose; 1 mg/kg BW), TL (transported + D-lactose; 1 mg/kg BW), TC (transported + chromium; 0.5 mg/kg dry matter [DM] feed), TM (transported + meloxicam; 1 mg/kg BW), and TMC (transported + combination of meloxicam and chromium; 1 mg/kg BW and 0.5 mg/kg DM, respectively). Doses of D-lactose monohydrate, meloxicam, and chromium were prepared for oral administration by suspension in 15 mL of water in a 20-mL dosing syringe. Blood was collected before transportation, immediately after 120 km of transportation (IAT), and at 6, 24, and 48 h after transportation. Results: Neither transportation nor administration of meloxicam and/or chromium affected (p = 0.99) average daily gain and feed intake. Plasma cortisol concentrations in the NL group (average 0.13 and 0.18 nmol/L, respectively) were lower (p<0.001) compared to the TL group (average 0.39 and 0.61 nmol/L, respectively) at IAT and 48 h after transportation. At 48 h after transportation, cortisol concentrations were lower (p<0.05) in the TC group (average 0.22 nmol/L) than in the TL group (average 0.61 nmol/L), and TC calves had similar cortisol concentrations to NL calves. Lying duration (min/d) was shorter (p<0.05) in the TL group than in the NL group at 2 d after transportation. Lying duration was longer (p<0.05) for the TC and TMC groups than for the TL group at 2 d after transportation. Conclusion: Transportation increased cortisol concentrations and affected lying behavior, while chromium administration reduced cortisol concentrations and changed lying behavior. Thus, chromium administration before transportation may be a viable strategy to alleviate stress elicited by road transportation.

Monitoring of Moisture and Dimensional Behaviors of Nail-Laminated Timber (NLT)-Concrete Slab Exposed to Outdoor Air

  • HWANG, Sung-Wook;CHUNG, Hyunwoo;LEE, Taekyeong;AHN, Kyung-Sun;PANG, Sung-Jun;BANG, Junsik;Won, Hyo;OH, Jung-Kwon;YEO, Hwanmyeong
    • Journal of the Korean Wood Science and Technology
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    • v.50 no.5
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    • pp.301-314
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    • 2022
  • The moisture and dimensional behaviors of a nail-laminated timber (NLT)-concrete slab composed of an NLT-plywood composite and topping concrete are monitored for 385 days. The slab is developed for using as flexural elements such as floors. The humidity control of wood gently introduces significant fluctuations under the ambient relative humidity into the slab, and fluctuations in the relative humidity result in dimensional changes. The equilibrium moisture content of the slab increases from 6.7% to 15.3% during the monitoring period, resulting in a width (radial) strain of 0.58%. The length (longitudinal) strain is negligible, and the height (tangential) strain is excluded from the analysis because of abstruse signal patterns generated. Concrete pouring causes a permanent increase in the width of the NLT-plywood composite. However, the width deforms because the weight of the concrete mixture loosens the nail-laminated structure, not because of the significant amount of moisture in the mixture. The dimensional stabilization effect of the nail-laminated system is demonstrated as the composite strain is lower than the total strain of lumber and plywood, which are elements constituting the nail-laminated structure.

Administration of chromium picolinate and meloxicam alleviates regrouping stress in dairy heifers

  • Da Jin Sol Jung;Jaesung Lee;Do Hyun Kim;Seok-Hyeon Beak;Soo Jong Hong;In Hyuk Jeong;Seon Pil Yoo;Jin Oh Lee;In Gu Cho;Dilla Mareistia Fassah;Hyun Jin Kim;Mohammad Malekkhahi;Myunggi Baik
    • Animal Bioscience
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    • v.37 no.8
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    • pp.1495-1502
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    • 2024
  • Objective: This research investigated the effect of administering chromium (Cr) and meloxicam (MEL) on growth performance, cortisol and blood metabolite, and behaviors in young, regrouped heifers. Methods: Fifty Holstein dairy heifers (body weight [BW] 198±32.7 kg and 6.5±0.82 months of age) were randomly assigned to non-regrouped group or four regrouped groups. Non-regrouped animals were held in the same pen throughout the entire experimental period (NL: non-regrouping and administration of lactose monohydrate [LM; placebo]). For regrouping groups, two or three heifers maintained in four different pens for 2 weeks were regrouped into a new pen and assigned to one of four groups: regrouping and LM administration (RL); regrouping and Cr administration (RC); regrouping and MEL administration (RM), and regrouping and Cr and MEL administration (RCM). LM (1 mg/kg BW), Cr (0.5 mg Cr picolinate/kg dry matter intake), and MEL (1 mg/kg BW) were orally administered immediately before regrouping. Blood was collected before regrouping (0 h) and at 3, 9, and 24 h and 7 and 14 d thereafter. Behaviors were recorded for 7 consecutive days after regrouping. Results: Average daily gain was lower (p<0.05) in RL than NL heifers, but was higher (p<0.05) in RM, RC, and RCM than RL heifers. RL heifers had higher (p<0.05) cortisol than NL heifers on d 1 after regrouping. The cortisol concentrations in RC, RM, and RCM groups were lower (p<0.05) than in RL treatment 1 d after regrouping. Displacement behavior was greater (p<0.05) in RL group than all other groups at 2, 3, and 6 d after regrouping. Conclusion: Regrouping caused temporal stress, reduced growth performance, and increased displacement behavior in heifers. Administering Cr and MEL recovered the retarded growth rate and reduced displacement behavior, thereby alleviating regrouping stress.

Induction of Inflammation Inhibits Taurine Transporter Activity in Murine Macrophage Cell Line

  • Kim, Jung-Hyun;Kim, Soyoung;Kim, Ha-Won;Kim, Byong-Kak
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1998.11a
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    • pp.156-157
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    • 1998
  • Taurine is synthesized in the body or uptaken from dietary and is distributed in the various organs. It differs from other amino acids by virtue of the fact that a sulfonic acid group replaces the carboxyl group of what would be ${\beta}$-alanine. In order to function within the cell it must be transported into the cells by taurine transporter that is spanned 12 transmembrane domains. The human taurine transporter has long cytoplasmic carboxy and amino termini that may function as regulatory attachment sites for other proteins. Six potential protein kinase C(PKC) phosphorylation sites have been reported in human taurine transporter.

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