• Journal of Microbiology and Biotechnology
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• v.24 no.7
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• pp.987-997
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• 2014

The mature landfill leachate, which is characterized by a high concentration of ammonia nitrogen ($NH_3$-N) and humic acid (HA), poses a challenge to biotreatment methods, due to the constituent toxicity and low biodegradable fraction of the organics. In this study, we applied bioaugmentation technology in landfill leachate degradation by introducing a domesticated $NH_3$-N and HA resistant bacteria strain, which was identified as Bacillus cereus (abbreviated as B. cereus Jlu) and Enterococcus casseliflavus (abbreviated as E. casseliflavus Jlu), respectively. The isolated strains exhibited excellent tolerant ability for $NH_3$-N and HA and they could also greatly improved the COD (chemical oxygen demand), $NH_3$-N and HA removal rate, and efficiency of bioaugmentation degradation of landfill leachate. Only 3 days was required for the domesticated bacteria to remove about 70.0% COD, compared with 9 days' degradation for the undomesticated (autochthonous) bacteria to obtain a similar removal rate. An orthogonal array was then used to further improve the COD and $NH_3$-N removal rate. Under the optimum condition, the COD removal rate in leachate by using E. casseliflavus Jlu and B. cereus Jlu increased to 86.0% and 90.0%, respectively after, 2 days of degradation. The simultaneous removal of $NH_3$-N and HA with more than 50% and 40% removal rate in leachate by employing the sole screened strain was first observed.

• Proceedings of the KSME Conference
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• 2001.11a
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• pp.129-136
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• 2001

The remaining life estimation for the aged components in power plants as well as chemical plants are very important beacuse mechanical properties of the components are degraded with time of service exposure in high temperature. Since it is difficult to take specimens from the operating components to evaluate mechanical properties of components, nondestructive techniques are needed to estimate the degradation. In this study, test materials with 4 different degradation levels were prepared by isothermal aging heat treatment at $630^{\circ}C$. And the DC potential drop method and destructive methods such as tensile, $K_{IC}$ and hardness tests were used in order to evaluate the degradation of 1Cr-1Mo-0.25V steels. The objective of this study is to investigate the possibility of the application of DCPD method to estimate the material degradation, and to analyse the relationship between the electrical resistivity and the degree of material degradation.

• Journal of the Korea Institute of Military Science and Technology
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• v.18 no.3
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• pp.267-274
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• 2015

It is necessary to both analyze root-cause of non-conformance of effective illumination time to the specification, and estimate the storage lifetime for 81 mm illuminating projectile stockpiled over 10 years. In this paper, aging mechanism of magnesium flare material due to long-term storage was supposed, and two-stage tests, pre-test and main test based on accelerated degradation tests were performed. Field storage environment of moistureproof was set up, and illumination times in the accelerated degradation tests for temperatures 60 and $70^{\circ}C$ were measured. Then, storage reliability of the projectile was estimated through analyzing the measured data and applying distribution-based degradation models to the data. The $B_{10}$ life by which 10 % of a population of the projectiles will have failed at storage temperature of $25^{\circ}C$ was estimated about 7 years.

• Korean Journal of Materials Research
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• v.33 no.11
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• pp.447-457
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• 2023

Single-atom Pd clusters anchored on t-BaTiO₃ material was synthesized using hydrothermal and ultrasonic methods for the effective piezoelectric catalytic degradation of pollutants using vibration energy. XRD patterns of BaTiO₃ loaded with monoatomic Pd were obtained before and after calcining, and showed typical cubic-phase BTO. TEM and HAADF-STEM images indicated single-atom Pd clusters were successfully introduced into the BaTiO₃. The piezoelectric current density of the prepared Pd-BaTiO₃ binary composite was significantly higher than that of the pristine BaTiO₃. Under mechanical vibration, the nanomaterial exhibited a tetracycline decomposition rate of ~95 % within 7 h, which is much higher than the degradation rate of 56.7 % observed with pure BaTiO₃. Many of the piezo-induced electrons escaped to the Pd-doped BaTiO₃ interface because of Pd's excellent conductivity. Single-atom Pd clusters help promote the separation of the piezo-induced electrons, thereby achieving synergistic catalysis. This work demonstrates the feasibility of combining ultrasonic technology with the piezoelectric effect and provides a promising strategy for the development of ultrasonic and piezoelectric materials.

• BMB Reports
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• v.45 no.3
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• pp.183-188
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• 2012

Participates in actin remodeling through Rac and receptor endocytosis via Rab5. Here, we used yeast two-hybrid system with Eps8 as bait to screen a human brain cDNA library. ITSN2 was identified as the novel binding factor of Eps8. The interaction between ITSN2 and Eps8 was demonstrated by the in vivo co-immunoprecipitation and colocalization assays and the in vitro GST pull-down assays. Furthermore, we mapped the interaction domains to the region between amino acids 260-306 of Eps8 and the coiled-coil domain of ITSN2. In addition, protein stability assays and immunofluorescence analysis showed ITSN2 overexpression induced the degradation of Eps8 proteins, which was markedly alleviated with the lysosome inhibitor NH4Cl treatment. Taken together, our results suggested ITSN2 interacts with Eps8 and stimulates the degradation of Eps8 proteins.

• Journal of Microbiology and Biotechnology
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• v.16 no.8
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• pp.1306-1310
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• 2006

Sphingomonas sp. strain SB5 could degrade carbofuran and carbofuran-7-phenol to a hydrolytic product, 2-hydroxy-3-(3-methlypropan-2-o1)phenol, and several red metabolites. However, the chemical structures of the red metabolites have largely remained unidentified. In this study, we identified the structure of one of the red metabolites as 5-(2-hydroxy-2-methyl-propyl)-2,2-dimethyl- 2,3-dihydro-naphtho[2,3-6]furan-4,6,7,9-tetrone by using mass spectrometric and NMR ($^1$H, $^{13}$C) analyses. It is suggested that the red metabolite resulted from condensation of some metabolites in the degradation of 2-hydroxy-3-(3-methlypropan-2-o1)phenol, a hydrolytic product derived from carbofuran. To our knowledge, this is the first paper to report a red metabolite in bacterial degradation of the insecticide carbofuran.

• Proceedings of the KSME Conference
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• 2000.04a
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• pp.37-42
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• 2000

The boiler tubes and steam Pipes operating both at high temperature and pressure for a long period of time in a power plant are degraded by creep because of internal pressure. So, the remaining life of a component is evaluated by the creep rupture strength. Although the conventional method to evaluate the creep damage is widely used, it has some disadvantages such as requires large size specimen and long employed to evaluate the correlation between fracture toughness and evaluation time. Recently, new method so called "small lunch test' is used to evaluate degradation of creep. In this study, a conventional creep test and a small punch test are conducted using 2.25Cr-1Mo steel which is mainly used for the boiler tubes and steam pipes in power plant. The creep life, approximately 1,500 hrs, is determined by conventional method under a severe condition then specimens for a small Punch test are obtained after certain time intervals such as 1/4, 1/2 and 3/4 of final rupture time, respectively.

• Journal of Applied Reliability
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• v.15 no.3
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• pp.154-162
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• 2015

We have performed accelerated photo-degradation test using a 10-Sun level high irradiance $Weather-Ometer^{(R)}$ (10-Sun Ci5000) in an attempt to study acceleration and correlation between accelerated and service conditions for ink-jet ink. The accelerated test was used to predict lifetimes of ink-jet ink through the calculation of scaling factor for intensity of irradiance and duration of usage combined with estimation of lifetime distribution and inverse power model as a life-stress model. The lifetimes and acceleration factors for foreign and domestic inks were compared with each other. The results showed that the failure mechanisms and life-stress models for ink-jet ink were different among the color of ink which means that we might be in need of further study by color of inks.

• Elastomers and Composites
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• v.56 no.3
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• pp.124-135
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• 2021

Evaluating service lives of rubber materials at certain temperatures requires a destructive method (typically using elongation at break). In this study, a non-destructive method based on hardness change rate was proposed for evaluating the service life of chloroprene rubber (CR). Compared to the destructive method, this non-destructive method ensures homogeneity of CR specimens and requires a small number of samples. Thermal accelerated degradation test was conducted on the CR specimens at 55, 70, 85, 100, and 125℃, and the tensile strength, elongation at break, and hardness were measured. The results of the experiment were compared to those of the accelerated life evaluation method proposed in this study. Comparing the analyzed lives in the high temperature region (70, 85, 100, and 125℃), the difference between the service lives for the destructive method (using the elongation at break) and non-destructive method (using the hardness) was approximately 0.1 year. Therefore, it was confirmed that the proposed non-destructive evaluation method based on hardness changes can evaluate the actual life of CR under thermally accelerated degradation conditions.

• Korean Journal of Agricultural Science
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• v.49 no.4
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• pp.995-1001
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• 2022

The purpose of this study was to compare the effect of solid-state fermentation on soybean using three microbial strains under four different fermentation times. Soybean was fermented for 12, 24, 36 or 48 hours with highly proteolytic microbes, either Bacillus amyloliquefaciens (BA), B. subtilis (BS), or B. subtilis var. natto (BN), and levels of total protein concentration, protein distribution, and antioxidant activity were analyzed. Total protein was highest in the BS 12 h group (9.21 ㎍·µL^-1) and lowest in BN 48 h (6.80 ㎍·µL^-1), respectively (p < 0.001). Furthermore, three microbes decomposed large molecular weight proteins as well as major allergens of soybean such as β-conglycinin, Gly m Bd 30K, and glycinin. Each treatment group showed the highest degradation rate at 48 h fermentation and among the three microbes, BS showed a relatively higher degradation rate. The radical scavenging ability, known as an indicator of antioxidant activity, showed a significant increase in all treatment groups except BA 24 h. The results from this study suggest that protein concentration, and degradation and antioxidant activity were affected by different types of microbial trains and fermentation period and that B. subtilis fermentation might be the most effective way to increase nutritional and functional properties of soybean.