• Natural Product Sciences
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• v.15 no.2
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• pp.71-75
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• 2009

The great economic and social damage caused by unusual explosion of jellyfish population has attracted the attention of researchers. A chemical study on the bioactive components of the giant jellyfish Nemopilema nomurai led to the isolation of two new (1 and 2) and three known alkoxyglycerols (3 - 5), along with known monoglycerides (6 - 7) and fatty acids. Based on NMR and MS data, the structures of compounds 1 and 2 were elucidated as 1-O-[(Z)-tetradec-3-enyl]-sn-glycerol and 1-O-[(Z)-octadec-10-enyl]-sn-glycerol, respectively. The absolute configurations of compounds 1 - 7 were determined by comparison of specific optical rotation values with those reported. The isolated compounds were evaluated for suppressive effect on the proinflammatory mediators (NO, IL-6, and TNF-${\alpha}$) in murine macrophage cells. However, they were found inactive upto the concentration of 100 ${\mu}M$.

• Structural Engineering and Mechanics
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• v.43 no.2
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• pp.199-209
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• 2012

Two carbon fiber composite laminates, $[0/90]_{2S}$ and $[0/+45/90/-45]_S$, were considered in this work to find out the perforation threshold energy to complete the perforation process and the corresponding maximum contact force. Explicit finite element commercial software, LS-DYNA, was used to predict these values. According to the simulation results, these two types of composite laminates were tested by using a vertical drop-weight testing machine. After testing, the damage condition of these specimens were observed and compared with the results from finite element analysis. The testing results indicate that the perforation threshold energy is 6 Joules for $[0/90]_{2S}$ and 7 Joules for $[0/+45/90/-45]_S$, which is in good agreement with the simulation results. Also, the maximum contact force at the case of perforation threshold energy is the lowest as compared to the maximum contact forces occurring at the impact energy that is larger or less than the perforation threshold energy.

• Steel and Composite Structures
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• v.20 no.6
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• pp.1369-1389
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• 2016

This paper investigates the flexural stiffness of simply supported steel-concrete composite I-beams under positive bending moment through combined experimental, numerical, and different standard methods. 14 composite beams are tested for experimental study and parameters including shear connection degree, transverse and longitudinal reinforcement ratios, loading way are also investigated. ABAQUS is employed to establish finite element (FE) models to simulate the flexural behavior of composite beams. The influences of a few key parameters, such as the shear connection degree, stud arrangement, stud diameter, beam length, loading way, on the flexural stiffness is also studied by parametric study. In addition, three widely used standard methods including GB, AISC, and British standards are used to estimate the flexural stiffness of the composite beams. The results are compared with the experimental and numerical results. The findings have provided comprehensive understanding of the flexural stiffness and the modelling of the composite beams. The results also indicate that GB 50017-2003 could provide better results in comparison to the other standards.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• v.2
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• pp.251-254
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• 2006

A precise real-time method of using the IGS ultra rapid products (IGU) and the GPS broadcast ephemeris to calculate the VRS orbit corrections was presented here which was suited for GPS/VRS reference station network based positioning. Test data acquired from both the SGRSN (Sichuan GPS Reference Station Network) and SCIGN (Southern California integrated GPS network) were used to evaluate the performance of the modeling techniques. The new method was proven to be more precise and reliable compared with the existing conventional network-based orbit error interpolation method. It was shown that 0.004ppm relative accuracy was reached, namely the influence from the orbit bias for the RTK positioning within 100km area can be of sub-millimeter level.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.8 no.1
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• pp.305-325
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• 2014

In cloud computing infrastructure, current virtual machine trust measurement methods have many shortcomings in dynamism, security and concurrency. In this paper, we present a new method to measure the trust of virtual machine. Firstly, we propose "behavior trace" to describe the state of virtual machine. Behavior trace is a sequence of behaviors. The measurement of behavior trace is conducted on the basis of anticipated trusted behavior, which not only ensures security of the virtual machine during runtime stage but also reduces complexity of the trust measurement. Based on the behavior trace, we present a Dynamic Tree Style Trust Measurement Model (DTSTM). In this model, the measurement of system domain and user domain is separated, which enhances the extensibility, security and concurrency of the measurement. Finally, based on System Call Interceptor (SCI) and Virtual Machine Introspection (VMI) technology, we implement a DTSTM prototype system for virtual machine trust measurement. Experimental results demonstrate that the system can effectively verify the trust of virtual machine and requires a relatively low performance overhead.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.4
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• pp.1693-1697
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• 2012

Objective: To explore the effect on radiosensitivity of arsenic trioxide ($As_20_3$) in conjunction with hyperthermia on the esophageal carcinoma EC-1 cell line. Method: Inhibition of EC-1 cell proliferation at different concentrations of $As_20_3$ was assessed using the methyl thiazolyl blue colorimetric method (MTT method), with calculation of $IC_{50}$ value and choice of 20% of the $IC_{50}$ as the experimental drug concentration. Blank control, $As_20_3$, hyperthermia, radiotherapy group, $As_20_3$ + hyperthermia, $As_20_3$ + radiotherapy, hyperthermia + radiotherapy and $As_20_3$ + hyperthermia + radiotherapy groups were established, and the cell survival fraction (SF) was calculated from flat panel colony forming analysis, and fitted by the 'multitarget click mathematical model'. Flow cytometry (FCM) was used to detect changes in cell apoptosis and the cell cycle. Results: $As_20_3$ exerted inhibitory effects on proliferation of esophageal carcinoma EC-1 cells, with an $IC_{50}$ of 18.7 ${\mu}mol/L$. After joint therapy of $As_20_3$ + hyperthermia + radiotherapy, the results of FCM showed that cells could be arrested in the $G_2$/M phase, and as the ratio of cells in $G_0/G_1$ and S phases decreased, cell death became more pronounced. Conclusion: $As_20_3$ and hyperthermia exert radiosensitivity effects on esophageal carcinoma EC-1 cells, with synergy in combination. Mechanistically, $As_20_3$ and hyperthermia mainly influence the cell cycle distribution of EC-1 esophageal carcinoma cells, decreasing the repair of sublethal damage and inducing apoptosis, thereby enhancing the killing effects of radioactive rays.

• Journal of Ginseng Research
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• v.42 no.3
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• pp.270-276
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• 2018

Background: Notoginsenoside Ft1 is a promising potential candidate for cardiovascular and cancer disease therapy owing to its positive pharmacological activities. However, the yield of Ft1 is ultralow utilizing reported methods. Herein, an acid hydrolyzing strategy was implemented in the acquirement of rare notoginsenoside Ft1. Methods: Chemical profiles were identified by ultraperformance liquid chromatography coupled with quadruple-time-of-flight and electrospray ionization mass spectrometry (UPLC-Q/TOF-ESI-MS). The acid hydrolyzing dynamic changes of chemical compositions and the possible transformation pathways of saponins were monitored by ultrahigh-performance LC coupled with tandem MS (UHPLC-MS/ MS). Results and conclusion: Notoginsenoside Ft1 was epimerized from notoginsenoside ST4, which was generated through cleaving the carbohydrate side chains at C-20 of notoginsenosides Fa and Fc, and vinaginsenoside R7, and further converted to other compounds via hydroxylation at C-25 or hydrolysis of the carbohydrate side chains at C-3 under the acid conditions. High temperature contributed to the hydroxylation reaction at C-25 and 25% acetic acid concentration was conducive to the preparation of notoginsenoside Ft1. C-20 epimers of notoginsenoside Ft1 and ST4 were successfully separated utilizing solvent method of acetic acid solution. The theoretical preparation yield rate of notoginsenoside Ft1 was about 1.8%, which would be beneficial to further study on its bioactivities and clinical application.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.27 no.11
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• pp.696-701
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• 2014

In this study, in order to develop coupled vibration mode piezoelectric devices for Acoustic Emission (abbreviated as AE) sensor application with outstanding displacement and piezoelectric properties have been simulated by ATILA FEM program. And, From the results of ATILA simulation, the AE sensor specimen, obtained superior electromechanical coupling factor and displacement, when the size of specimen is $3.45mm{\Phi}{\times}3.45mm$ with ratio of diameter/thickness(${\Phi}/T$)= 1.0. Therefore, AE sensor was fabricated by (Na,K,Li)(Nb,Ta) $O_3$(abbreviated as NKL-NT) system piezoelectric ceramics using coupled vibration mode. The piezoelectric properties of NKL-NT ceramics was exhibited that piezoelectric constant($d_{33}$), piezoelectric voltage constant($g_{33}$) and electro mechanical coupling factor($k_p$) have the excellent values of 261[pC/N], 40.10[$10^{-3}Vm/N$], and 0.44, respectively. The manufactured piezoelectric device with ratio of ${\Phi}/T$= 1.0 indicated the optimum values of resonant frequency(fr)= 556.5[kHz], antiresonant frequency(fa)=631.1[kHz], and effective electromechanical coupling factor(keff)= 0.473. The maximum sensitivity of the coupled vibration mode AE sensor was 55[dB] at the resonant frequency of 75[kHz]. The results show that the coupled vibration mode piezoelectric device is a promising candidate for the application AE sensor piezoelectric device.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.2
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• pp.571-578
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• 2015

Prostate cancer is the most common cancer in men. In this study, we investigated immune responses of cytotoxic T lymphocytes (CTLs) against TRAMP-C2 prostate cancer cells after activation by dendritic cells (DCs) loaded with TRAMP-C2 freeze-thaw antigen and/or PEP-3 peptide in vitro. Bone marrow-derived DC from the bone marrow of the C57BL/6 were induced to mature by using the cytokine of rhGM-CSF and rhIL-4, and loaded with either the freeze-thaw antigen or PEP-3 peptide or both of them. Maturation of DCs was detected by flow cytometry. The killing efficiency of the CTLs on TRAMP-C2 cells were detected by flow cytometry, CCK8, colony formation, transwell migration, and wound-healing assay. The levels of the IFN-${\gamma}$, TNF-${\beta}$ and IL-12 were measured by enzyme-linked immunosorbent assay (ELISA). Compared with the unloaded DCs, the loaded DCs had significantly increased expression of several phenotypes related to DC maturation. CTLs activated by DCs loaded with freeze-thaw antigen and PEP-3 peptide had more evident cytotoxicity against TRAMP-C2 cells in vitro. The secretion levels of IFN-${\gamma}$, TNF-${\beta}$ and IL-12, secreted by DCs loaded with antigen and PEP-3 and interaction with T cells, were higher than in the other groups. Our results suggest that the CTLs activated by DCs loaded with TRAMP-C2 freeze-thaw antigen and PEP-3 peptide exert a remarkable killing efficiency against TRAMP-C2 cells in vitro.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.1
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• pp.523-528
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• 2013

Background: The possibility that electromagnetic fields (EMF) exposure may increase male breast cancer risk has been discussed for a long time. However, arguments have been presented that studies limited by poor quality could have led to statistically significant results by chance or bias. Moreover, data fo the last 10 years have not been systematically summarized. Methods and Results: To confirm any possible association, a meta-analysis was performed by a systematic search strategy. Totals of 7 case-control and 11 cohort studies was identified and pooled ORs with 95% CIs were used as the principal outcome measures. Data from these studies were extracted with a standard meta-analysis procedure and grouped in relation to study design, cut-off point, exposure assessment method, adjustment and exposure model. A statistical significant increased risk of male breast cancer with EMF exposure was defined (pooled ORs = 1.32, 95% CI = 1.14-1.52, P < 0.001), and subgroup analyses also showed similar results. Conclusions: This meta-analysis suggests that EMF exposure may be associated with the increase risk of male breast cancer despite the arguments raised.