In this study, hydroxyapatite (HAp) was incorporated into toothpaste and its effect on the remineralization and restoration of dental enamel was evaluated. Different sets of toothpaste were incorporated with HAp levels of 0%, 5%, 10 %, and 15 %. The filler particles of the resulting toothpaste samples were observed via SEM and XRD and compared with compositions of several commercially available toothpastes, showing that the HAp was successfully incorporated into the toothpaste samples. Different sets of human enamel were inflicted with lesions and then treated with the different fabricated toothpaste samples for five minutes three times a day for seven days. During the treatment, the teeth were subjected to demineralization and remineralization cycles to simulate the effect of natural saliva. The surface of the enamel samples were observed using SEM before and after one week of treatment, showing the formation of HAp layers on the surfaces of the enamel samples. The effect of the toothpaste on the lesions was observed using an inverted light microscope and the lesion depth was found to decrease as the concentration of HAp in the toothpaste used increased. HAp was successfully incorporated in the toothpaste and its presence was found to lessen lesion depths and improve tooth remineralization.
A non-carious cervical lesion(NCCL) is the loss of tooth structure at the cementoenamel junction level that is unrelated to dental caries. This study was to evaluate the occlusal and periodontal status of teeth with non-carious cervical lesions. We evaluated 105 teeth with non-carious cervical lesions in 35 subjects aged 38-75 years and characterized them based on the shape and dimension, plaque retention, bleeding on probing(BOP), probing pocket depth(PPD), occlusal status, brushing type, hypersensitivity and wear facet. The results of this study were as follows 1. No significant association was observed between cervical lesions and occlusal contact in lateral excursions. 2. No significant difference occurred in plaque retention, PPD, BOP between teeth with and without cervical lesions. 3. Test teeth had a significantly higher percentage of hypersensitivity and occlusal wear facet than teeth without cervical lesions. 4. Wedge shaped lesions had a significantly higher percentage of plaque than saucer shaped lesions. 5. Teeth with plaque were found to have significantly deeper PPD than teeth without plaque retention in cervical regions. 6. Teeth with occlusal contacts were found to have significantly deeper PPD than teeth without occlusal contacts. 7. No significant association was observed between cervical lesions and PPD independent of plaque retention and occlusal contacts Although more knowledge is necessary, our results suggest that occlusal contact and bacterial plaque may influence on periodontal tissue, but NCCL is not directly associated with periodontal health
Stomach cancer has a high annual mortality rate worldwide necessitating early detection and accurate treatment. Even experienced specialists can make erroneous judgments based on several factors. Artificial intelligence (AI) technologies are being developed rapidly to assist in this field. Here, we aimed to determine how AI technology is used in gastric cancer diagnosis and analyze how it helps patients and surgeons. Early detection and correct treatment of early gastric cancer (EGC) can greatly increase survival rates. To determine this, it is important to accurately determine the diagnosis and depth of the lesion and the presence or absence of metastasis to the lymph nodes, and suggest an appropriate treatment method. The deep learning algorithm, which has learned gastric lesion endoscopyimages, morphological characteristics, and patient clinical information, detects gastric lesions with high accuracy, sensitivity, and specificity, and predicts morphological characteristics. Through this, AI assists the judgment of specialists to help select the correct treatment method among endoscopic procedures and radical resections and helps to predict the resection margins of lesions. Additionally, AI technology has increased the diagnostic rate of both relatively inexperienced and skilled endoscopic diagnosticians. However, there were limitations in the data used for learning, such as the amount of quantitatively insufficient data, retrospective study design, single-center design, and cases of non-various lesions. Nevertheless, this assisted endoscopic diagnosis technology that incorporates deep learning technology is sufficiently practical and future-oriented and can play an important role in suggesting accurate treatment plans to surgeons for resection of lesions in the treatment of EGC.
There are considerable in vitro and in vivo evidences for remineralization and demineralization occurring simultaneously in incipient enamel caries. In order to "heal" the incipient dental caries, many experiments have been carried out to determine the optimal conditions for remineralization. It was shown that remineralization is affected by different pH, lactic acid concentrations, chemical composition of the enamel, fluoride concentrations, etc. Eighty specimens from sound permanent teeth without demineralization or cracks, 0.15 mm in thickness, were immersed in lactic acid buffered demineralization solutions for 3 days. Dental caries with a surface zone and subsurface lesion were artificially produced. Groups of 10 specimens were immersed for 10 or 12 days in lactic acid buffered remineralization solutions consisting of pH 4.3 or pH 6.0, and 100, 50, 25, or 10 mM lactic acid. After demineralization and remineralization, images were taken by polarizing microscopy (${\times}100$) and micro-computed tomography. The results were obtained by observing images of the specimens and the density of the caries lesions was determined. 1.As the lactic acid concentration of the remineralization solutions with pH 4.3 was higher, the surface zone of the carious enamel increased and an isotropic zone of the subsurface lesion was found. However, the total decalcification depth increased at the same time. 2.In the remineralization solutions with pH 6.0, only the surface zone increased slightly but there was no significant change in the total decalcification depth and subsurface zone. In the lactic acid buffer solutions with the lower pH and higher lactic acid concentration, there were dynamic changes at the deep area of the dental carious lesion.
Journal of the korean academy of Pediatric Dentistry
/
v.37
no.1
/
pp.35-43
/
2010
We have developed a sodium fluoride containing gelatin and methyl cellulose gel. Cariostatic abilities of those gel were investigated and compared with APF gel and fluoride varnish($Cavityshield^{TM}$). We prepared the bovine tooth samples and divided into two surface, control side and experimental side in same specimen for exclusion of difference between specimens. The experiment was consisted of 4 groups : (I) APF gel : (II) $Cavityshield^{TM}$ : (III) Gelatin F gel : (IV) Methyl cellulose F gel Decalcification were produced by placing each specimen into artificial acidic solution(pH 4.0) for 72 hours. Surface microhardness were measured and depth of demineralization lesion were measured by polarizing light microscope. The results were as follows: 1. The difference of VHN between control and experimental side is smallest in group I (p<0.05). 2. The largest difference was shown in group II (p<0.05). 3. There were no significant difference between group III and IV in microhardness test (p>0.05). 4. The difference of lesion depth is smallest in group I (p<0.05). 5. There were no significant difference between group II, III and IV in lesion depth (p>0.05). The result of the present study indicate that the fluoride containing gelatin and methyl cellulose gel is more effective than APF gel and is similar to fluoride varnish application for prevention of demineralization.
Lesion enlargement of ginger rhizome rot was most rapid at 35~40 C, but delayed greatly as temperature decreased. Time needed for a killing a ginger plant, 22~25 cm long, was about 5 days at 35~40 C, but was 15 days at 15 C in a growth chamber test. Higher RH above 90%, higher soil moisture level above 80% of maximum soil moisture capacity, and deeper planting below 4cm enhanced the lesion development on ginger stems and rhizomes. Pythium myriotylum existed in field soil as forms of hyphal portion, hyphal swelling body, or oospore- or zoospore-like bodies, and served as the origin of its colonization. Inocula of P. myriotylum was randomly distributed in soil surface around ginger plants, but its density was decreased as increasing soil depth with the highest density at 0~10 cm soil depth. Population density of P. myriotylum did not vary significantly between the rhizoplane and the rhizosphere soil of a ginger plant, but differed greatly between the disessed and healthy plants with several to several hundreds times higher population in the diseased plants. A positive curvilinear relationship was found between P. myriotylum density and ginger rhizome rot severity.
Journal of the korean academy of Pediatric Dentistry
/
v.35
no.4
/
pp.662-670
/
2008
The purpose of this in vitro study was to evaluate the effect of fluoride containing adhesive film on prevention of enamel demineralization. Eighty bovine enamel blocks were divided randomly into 4 groups of 25: (1) APF gel applied ; (2) CavityShield$^{TM}$ applied; (3) 3% fluoride film applied; (4) 5% fluoride film applied; Early caries lesions were produced by placing each specimen into demineralization solution at pH 4.0 for 72 hours. Then lesion of the surface microhardness were measured by the Vicke's hardness test and the lesions depth were measured by polarizing light microscope. The results of the present study are as follows: 1. Difference of microhardness value ($M{\pm}SD$) between control and experimental side was the highest in group II, followed by group IV, III, I but, no significant difference was between group II, III and IV. 2. Difference of mean lesion depth ($M{\pm}SD$) between control and experimental side was the highest in group II, followed by group III, IV and I but no significant difference was between group III and IV. The results of the present study indicate that the fluoride film application is more effective than APF gel and is similar to fluoride varnish application for prevention of dental caries.
Purpose: Point fixation at the margin of the glenoid is a limitation of conventional arthroscopic stabilization using suture anchors, and does not afford sufficient footprint healing, especially in glenoid bone deficiency. So, we introduce an arthroscopic suture bridge transosseous-equivalent technique for bony Bankart lesions to avoid the technical disadvantage of point contact with anchor fixation and to improve mechanical stability through cross compression of the labrum. Surgical approach: The technique was adapted from the transosseous-equivalent rotator cuff repair technique using suture bridges, which improved the pressurized contact area and mean pressure between the tendon and footprint. After preparation of the glenoid bed by removal, reshaping, or mobilization of the bony lesion, two anchors (3.0 mm Biofastak, $Arthrex^{(R)}$, Naples, FL) were inserted into the superior and inferior portion of the bony Bankart lesion. Using a suture hook, medial mattress sutures were applied around the capsulolabral portion of the IGHL complex to obtain sufficient depth of glenoid coverage. A 3.5 mm pushloc anchor ($Arthrex^{(R)}$, Naples, FL) hole was made in the articular edge of the anterior glenoid rim. distal, suture bridge was applied, and proximal was inserted to mobilize the labrum in the proximal direction. This avoided the technical disadvantage of point contact with anchor fixation and decreased the level of gap formation through cross-compression of the labrum.
The periodontal flap surgery is the most widely utilized surgical procedure to reduce the pocket depth and to access the subgingival root surfaces for scaling and root planing. The diagnosis of the periodontal lesion and the objective of the surgery will dictate the type of flap procedure which will be utilized to obtain the best result. The incisions, type of flap and the selection of suturing design must be planned and executed to fit the problem. Periodontal flaps are designed to preserve gingival integrity and to gain access to root surfaces for residual calculus removal and to thoroughly remove granulation tissue so bone defects can be visualized and treated. Gentle and efficient procedures result in optimum healing and minimal postoperative pain. When flaps need to be repositioned apically or less often, coronally, then the flaps must sit passively at the appropriate level before suturing. To ensure this, buccal and lingual flaps need to be elevated beyond the mucogingival junction so the elasticity of the mucosa allows for flap mobility. Sometimes it may be necessary to extend the flap elevation apically with a split incision approach to minimize the effect of the less elastic periosteum. Vertical incisions can aid in flap positioning by allow ing the clinician to suture the flap at a different level to the adjacent untreated gingiva. In osseous periodontal surgery, flaps are apically positioned to minimize postoperative pocket depth. In regenerative periodontal surgery including implant surgery, soft tissue cove rage of bony defects, graft materials, membranes, and bio logic agents is important so sulcular incisions and light suturing techniques are crucial.
Purpose: The aim of this study was to show the clinical results of combination of Nd-YAP (1340nm) laser therapy with conventional endodontic and periodontal treatment. Materials and Methods: Four patients with chronic advanced periodontitis and endodontic infection were treated with conventional treatment and Nd-YAP laser therapy. Occlusal adjustment and splinting were done for stabilization of the teeth with severe horizontal and vertical mobility. The protocol for periodontal treatment was followed as scaling and root planing, pocket irrigation with 3% $H_2O_2$ and exposure of Nd-YAP laser using 320${\mu}m$ optical fiber with 160mJ/pluse, 30Hz. The other protocol for endodontic treatment was followed as access opening, canal preparation by hand and rotary instrument, canal filling, and exposure of Nd-YAP laser using 200${\mu}m$ optical fiber with 200mJ/pluse, 10Hz and 180mJ/pluse, 5Hz which were used respectively for disinfection and canal filling. The assessments of probing depth, mobility, and radiography were made prior to and after treatment. Result: All of these four clinical cases showed good healing of periodontium, which presented decrease of mobility and pocket depth, and increase of bone regeneration and bone density on the radiography. Conclusion: The bactericidal effect of Nd-YAP laser would provide benefits for improving clinical results that are obtained from conventional therapy.
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