• KSBB Journal
• /
• v.22 no.1
• /
• pp.1-6
• /
• 2007

The optimum liquid culture conditions were investigated for cell growth and polysaccharide production from liquid culture of Lentinus edodes. In flask culture, the optimal medium compositions for the polysaccharide production contained glucose 60 g/L, yeast extract 10 g/L, $KH_2PO_4$ 2.0 g/L, and $MgSO_4{\cdot}7H_2O$ 1.0 g/L. The maximum mycelial growth and polysaccharide production were 11.01 g/L and 1.64 g/L, respectively. In bioreactor, through the variation of aeration in order to increase mycelial growth and polysaccharide production, the maximum mycelial growth and polysaccharide production were 55.9 g/L at 8th day and 7.34 g/L at 7th day of cultivation with 1.5 vvm, respectively.

• Food Science and Preservation
• /
• v.10 no.1
• /
• pp.89-93
• /
• 2003

To develope natural food preservatives, ethanol and water extracts were prepared from the Lentinus edodes and antimicrobial activities were examined against 10 microorganisms which were food borne pathogens and / or food poisoning microorganisms and food-related bacteria and yeasts. Ethanol extract exhibited antimicrobial activities for the microorganisms tested, but not on lactic acid bacteria and yeast Especially, minimum inhibitory concentration(MIC) for Escherichia coli were as low as 0.5 mg/mL. Antimicrobial activity of the ethanol extract was stable by the heating at 121$^{\circ}C$ for 15 min and not affected by pH. The ethanol extract of Lentinus edodes exhibiting high antimicrobial activity. The highest antimicrobial activity adjust bacteria tested was found in the ethylacetate fraction.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2002.11b
• /
• pp.59-59
• /
• 2002

This study was excuted to decide the physiological characteristics of Lentinus lepides. L. lepides was tested to select pertinent substract, temperature and pH range for the growth. Mycelial growth of Lentinus lepides was mostly supported on MYPA among other tested synthetic or semi-synthetic media. The temperature range for pertinent mycelial growth was about 22~32$^{\circ}C$ and mostly stimulated at $25^{\circ}C$. And the pertinent pH range of MYPA was 3.0~4.0. The required carbon and nitrogen source for mycelial growth of Lentinus lepides was tested. The mycelial growth was mostly stimulated by soluble starch at content. The carbon sources for pertinent mycelial growth was maltose. And the nitrogen source for pertinent mycelial growth was peptone.

• Journal of environmental and Sanitary engineering
• /
• v.7 no.1
• /
• pp.57-66
• /
• 1992

To investigate on the element content of twelve edible mushrooms and Aloe arborescent, i.e., Lentinus edodes, Ganoderma lucidum (culturing in wood and soil), Tricholoma matsutake, Agaricus auricula-Jude (produced in Korea and China), sarcodon asparatus, Pleurotus ostreatus, Coriolus bisporus, Cyrophora esculenta, Auricularia auricula-Jude (produced in Korea and China), Sarcodon asparatus, Pleurotus ostreatus, Coriolus rersicolor, Smilax rotundifolia and Aloe arborescenstyl were analyzed by Atomic absorption spectrometer. The obtained results were summarized as follows : 1. Potassium, sodium, magnesium and iron content for the most part samples were in large quantities, especi phosphorus content of those was highest ammount for the all samples. 2. Sodium content f·as much ammount in the Lentinus edodes(39 mg, and Ganoderma lucidum(20 mg) , Culturing in wood and soil, while potassium was very high ammount in the Aloe arborescens and other samples. Mush ammount of magnesium as compared with others was Lentinus edodes(144 mg) , Ganoderma lucidum(128 mg), Aloe arborescens(50 mg) and Pleurotus ostreatus (60 mg) . 3, Phosphorus content of Lentinus edodes, Gyrophora esculenta, Auricularia polytricha and Agaricus bisporus was much ammount while iron content of all samples equality higher ammount. Sodium content of Aloe arborescens was not analyzed out for almost all, its potassium(82 mg), magnesium(50 mg) and iron(18 mg) content comparatively higher quentity than others nllnerals and phosphorus volume (4.9 mg) as compared with others, was conspicuously lower detect. 4. Cadimium and lead content of harmful metal element were detected on trace quantity for the most part samples. 5. Organic acids of samples i.e., Lentinus edodes, Agaricus bisporus, Pleuritus ostreatus and Ganoderma lucidum were Citrate, Malate, Fumalate, Succinate, Oxalate, Acetate, Lactate, and Tartarate and Citrate, Malate and Fumarate contents were higher amount remarkbly than other organic acids. Tartarate content was trace amount.

• Korean Journal of Microbiology
• /
• v.30 no.4
• /
• pp.252-259
• /
• 1992

Extracellular laccase excreted from Lentinus edodes ATCC 48085 was purified through a series of DEAF, Sephadex A-50. Con A-Sepharosc and Sephadex G-150 chromatography. Extracellular enzyme. which consists of a single polypeptide, has a n~olecular mass of 87.000 daltons and contains 12.0'%, carbohydrate. The N-terminal amino acid sequence (I5 residues) of the puritied enzyme was similar to that of laccases of PIeurotus ostreatus and Coriolus hirsutus. The enzyme showed optimal activity at near pH 4.8 and $40^{\circ}C$. The enzyme was stable at pH 7-9 and below $30^{\circ}C$. $K_{M}$ and $k_{cat}$ values for syringaldazine were estimated to be $0.4\mu\textrm{M}$ and 77 sec, respectively. The developed patterns of reaction products of thevenzyme on thin layer chromatography were similar to those of laccase of Pleurotus ostreatus.

• The Korean Journal of Mycology
• /
• v.12 no.4
• /
• pp.129-132
• /
• 1984

To find antitumor constituents in Korean basidiomycetes, the mycelia of Lentinus edodes DMC7, which had shown a good mycelial growth in shakeflasks, were cultured at $27^{\circ}C$ on an orbital shaking incubator at 180 rev/min for 12 days. The medium was composed of glucose (50g/l), yeast extract (9g/l), peptone (9g/l), and seven inorganic salts. A water soluble macromolecular fraction, LF-3, was obtained from the culure filtrate by fractionation with ethanol and dialysis using a Visking tube. When LF-3 was administered i.p. at 50mg/kg/day once daily for 10 consecutive days to female ICR mice which were implanted s.c. with sarcoma 180 $(10^6\;cells/mouse)$, it exerted a highly significant antitumor activity, with the tumor inhibition ratio of 53. 1%.

• Archives of Pharmacal Research
• /
• v.20 no.5
• /
• pp.443-447
• /
• 1997

As the growth factor of lactic acid bacteria, LD (trehalose) was isolated from Lentinus edode5 by using silica gel column chromatography. LD induced the growth of Bifidobacteria breve and Lactobacillus brevis, which were isolated from human feces. LD selectively induced the growth of lactic acid bacteria among total microflora. When total intestinal microflora were cultured in the medium containing LD, it stimulated the growth of lactic acid bacteria and inhibited harmful enzymes, ${\beta}$-glucosidase, ${\beta}$-glucuronidase, and tryptophanase, of intestinal bacteria. LM, which was a monosaccharide from L. edooles, induced the growth of lactic acid bacteria but it seems to be invaluable in vivo. LH isolated from L. edodes by Sephadex G-100 column chromatography was not effective for the growth of lactic acid bacteria.

• 한국생물공학회:학술대회논문집
• /
• 2003.10a
• /
• pp.667-671
• /
• 2003

The total phenolic and flavonoid contents of Lentinus edodes extracts were determinated by spectrophotometrical .method, and antihepatotoxic activity was detected on glutathione S-Transferases(GST). The total phenolic contents was highest water extract than solvent(ethanol, methanol) extracts, but flavonoid content was appear on opposite. GST activity was the highest appears in water extract. This fact verified of anticancer effect indirectly of Lentinus edodes.

• 한국생물공학회:학술대회논문집
• /
• 2003.04a
• /
• pp.209-212
• /
• 2003

This study was to investigate the effects of media components on carbohydrolase complex production in liquid culture of Lentinus edodes. Glucose and yeast extract, and $MgSO_4\;.\;7H_2O$ were selected as carbon, nitrogen, and mineral sources, respectively. The production level of ${\beta}-glucosidase$, one of carbohydrolase complex, was high. On the other hand, ${\beta}-glucuronidase$ and ${\beta}-galactosidase$ production levels were very low, which remain to be improved.

• Mycobiology
• /
• v.48 no.1
• /
• pp.51-57
• /
• 2020

A polysaccharide (LVP) was purified from fruiting body of Lentinus velutinus by ethanol precipitation fractionation and DEAE and Sephadex G-100 column chromatography. The yield of purified polysaccharide was 0.025%. Molecular characteristics of LVP were determined by gel permeation chromatography, FT-IR spectroscopy, and thin-layer chromatography. Our results revealed that LVP is a polysaccharide composed of only glucose units, and has a molecular weight of 336 kDa. Biological activity assays indicated that LVP exhibits both cytotoxic and antioxidant activity. LVP showed specific cytotoxicity against cancer cells (HeLa and HepG2 cells), and alterations in cancer cell morphology were found after LVP treatment.