• Journal of Microbiology and Biotechnology
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• 제23권6호
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• pp.856-863
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• 2013

Application of rhizospheric fungi is an effective and environmentally friendly method of improving plant growth and controlling many plant diseases. The current study was aimed to identify phytohormone-producing fungi from soil, to understand their roles in sesame plant growth, and to control Fusarium disease. Three predominant fungi (PNF1, PNF2, and PNF3) isolated from the rhizospheric soil of peanut plants were screened for their growth-promoting efficiency on sesame seedlings. Among these isolates, PNF2 significantly increased the shoot length and fresh weight of seedlings compared with controls. Analysis of the fungal culture filtrate showed a higher concentration of indole acetic acid in PNF2 than in the other isolates. PNF2 was identified as Penicillium sp. on the basis of phylogenetic analysis of ITS sequence similarity. The in vitro biocontrol activity of Penicillium sp. against Fusarium sp. was exhibited by a 49% inhibition of mycelial growth in a dual culture bioassay and by hyphal injuries as observed by scanning electron microscopy. In addition, greenhouse experiments revealed that Fusarium inhibited growth in sesame plants by damaging lipid membranes and reducing protein content. Co-cultivation with Penicillium sp. mitigated Fusarium-induced oxidative stress in sesame plants by limiting membrane lipid peroxidation, and by increasing the protein concentration, levels of antioxidants such as total polyphenols, and peroxidase and polyphenoloxidase activities. Thus, our findings suggest that Penicillium sp. is a potent plant growth-promoting fungus that has the ability to ameliorate damage caused by Fusarium infection in sesame cultivation.

• Asian-Australasian Journal of Animal Sciences
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• 제16권10호
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• pp.1429-1437
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• 2003

Browse tree legume leaves from Acacia spp (A. nilotica, A. tortilis, A. polyacantha), Dichrostachys sp, Flagea villosa, Piliostigma thonningii, Harrisonia sp were evaluated for nutritive potential (chemical compositions and degradability characteristics) compared to Gliricidia sepium. Effect of tannins anti-nutritive activity on digestibility was also assessed by polyethylene glycol (PEG) tannin bioassay. Crude protein (CP), ash, neutral detergent fiber (NDF), acid detergent fiber (ADF) and acid detergent lignin (ADL) differed (p<0.05) between legume foliages. Mean CP, ash, NDF, ADF and ADL for fodder species tested were 158, 92, 385, 145, and 100 g/kg DM, respectively. CP ranged from 115 (P. thonningii) to 205 g/kg DM (G. sepium). Acacia spp had moderate CP values (g/kg DM) of 144 (A. nilotica), to high CP in A. tortilis (188) and A. polyacantha (194) comparable to G. sepium. The forages had relatively lower fiber compositions. A. nilotica had (p<0.05) lowest NDF, ADF and ADL (182, 68 and 44) compared to P. thonningii (619, 196 and 130) g/kg DM, respectively. Except G. sepium, all fodder species had detectable high phenolic and tannin contents greater than 5% DM, an upper beneficial level in animal feeding and nutrition. Mean total phenolics (TP), total tannins (TT) and condensed tannins (CT) (or proanthocyanidins) for fodder species tested were 139, 113 and 43 mg/g DM, respectively. F. villosa had (p<0.05) lowest TP and TT of 65 and 56 mg/g DM, respectively, compared to A. nilotica (237 and 236 mg/g DM, respectively). The CT varied (p<0.05) from 6 (F. villosa) to 74 mg/g DM (Dichrostachys sp). In vitro organic matter (OM) degradability (OMD) differed (p<0.05) between fodder species. G. sepium had (p<0.05) high degradability potential compared to A. polyacantha that had (p<0.05) the lowest OMD values. Forage degradability ranked: G. sepium>A. nilotica>P. thonningi>F. villosa>Dichrostachys sp>A. tortilis>A. polyacantha. Addition of PEG resulted to (p<0.05) improvement in in vitro OM digestibility (IVD). Increase in IVD was mainly due to binding action of PEG on tannins; and represents potential nutritive values previously depressed by tannins anti-nutritive activity. Browse fodder has potential as sources of ruminal nitrogen especially for ruminants consuming low quality roughages due to high protein, lower fiber compositions and high potential digestibility. However, utilization of browse supplements in ruminants is hampered by high phenolic and tannin contents. Deactivation of tannin anti-nutritive activity, possibly by feeding tanniniferous browse with other readily available nitrogen sources to dilute tannin anti-nutritive activity could improve utilization of browse fodder supplements. Further studies are needed to assess browse fodder palatability and intake, and their effect on growth performance in ruminants.

• The Korean Journal of Ecology
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• 제15권3호
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• pp.209-220
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• 1992

We investigated major plant communities and seed dispersal patterns in areas a(alt. 2,263 ~2,350m),b(alt. 2,350~2,420m), c(alt. 2,420m) with levels of a crater on mt. paektu. Thirty two species distributed around chunji consisted of 24 perennial herbs, 1 biennial herb and 7 alpine dwarf shurbs. Their fruit types were quite diverse; capsule(11), achene(10), grain(4), legume(2), follicle(2), schizocarp(2) and drupe(1). in area a, adjancent to the chunji(lake) water surface, gramineae sp., cyperaceae sp., chrysanthemum zawadskii and bistorta ochotensis were occured as members of the major plant communities. Species of gramineae and cyperaceae seem to have become dominant because their seeds float well. Astragalus membranaceus is legume and endures the winter cold and summer drought. in area c, alpine dwarf shrubs (e. g.rhododendron redowskianum, rhododendron aureum, empetrum nigrum) were dominant. In area c, compared to a and b, the weather is drier because of strong wind and lower water holding capacity of the soil. Therefore the pattern of plants distributing around chunji is different from that in western slope on mt.paektu. environmental factor such as wind, temperature and water may contribute to the pattern.

• Asian-Australasian Journal of Animal Sciences
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• 제7권3호
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• pp.325-333
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• 1994

This study was undertaken at Gowa, South Sulawesi, Indonesia as part of a larger forage genetic resource evaluation project. The experimental program consisted of a field experiment where grasses and legume species were grown in monocultures and the yield, N content and rumen bag digestibility using goats were measured. The field experiment was conducted from December 1985 to October 1986. Eight species of legumes (Desmodium heterophyllum cv. John-stone, Desmodium triflorum from Gowa, South Sulawesi, Arachis sp. from Maiwa, South Sulawesi, Clitoria ternalea CPI 50973, Macroptilium atropurpureum ev. Siratro, Neonotonia wightii cv. Tinaroo, Centrosema pubescens CPI 58575, Centrosema plumeri CPI 58568) were grown as monocultures. After establishment all plants were cut to a uniform height of 5 cm, and subsequent cuts were made on regrowth after 14, 28, 42, and 56 days (cycle 1). Cycle 2 commenced towards the end of the wet season and continued for 157 days into the dry season. The highest yielding legumes were C. ternatea in the wet season and Arachis sp. in the dry season. The mean rumen bag dry matter digestibility (RBDMD) of legumes of 67.6% for leaf material (averaged over all cycles and ages) was 7.6% higher than for stem material. The RBDMD of Arachis was significantly higher than all other species. The RBDMD of all legumes declined with age. Calculation of yield of digestible DM (yield $\times$ RBDMD) showed that Arachis sp. was the best legume. The combination of plant "quality" with yield measures is a valuable adjunct to routine agronomic survey procedures in plant evaluation programs. Arachis sp. appears to offer considerable promise and should be more widely evaluated.

• 한국토양비료학회지
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• 제25권4호
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• pp.378-386
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• 1992

Cowpea군(群) 두과작물(豆科作物)에 대한 땅콩근류균(根瘤菌) HCR-46의 근류형성능(根瘤形成能) 및 질소고정능(窒素固定能)을 검토(檢討)하고자 동부, 녹두, 팥, 땅콩에 무접종(無接種)+N : 0kg/10a, 무접종(無接種)+N : 6kg/10a, 접종(接種)+N : 0kg/10a, 접종(接種)+N : 6kg/10a, 접종(接種)+N : 12kg/10a로 처리(處理)하여 시험(試驗)한 결과(結果) 초기근류형성시기(初期根瘤形成時期)는 팥이 가장 빠르고 동부, 녹두, 땅콩순(順)이었으며 질소(窒素)를 증시(增施)할 수록 지연(遲延)되는 경향(傾向)이었다. 근류수(根瘤數)와 근류중(根瘤重)은 생육(生育)이 진행(進行)될 수록 증가(增加)하였고, 질소(窒素)를 증시(增施)할 경우 땅콩을 제외한 동부, 녹두, 팥에서 감소(減少)하였다. 건물생산량(乾物生産量)과 질소흡수량(窒素吸收量)은 균접종구(菌接種區)가 무접종구(無接種區)에 비(比)해 높았고 균접종시(菌接種時)는 질소무시용구(窒素無施用區)에 비(比)해 질소(窒素) 6kg/10a 시용구(施用區)가 높았다. 접종균(接種菌)에 의해 고정(固定)된 질소량(窒素量)은 파종후(播種後) 60일(日) 질소무시용구(窒素無施用區)의 동부, 녹두, 팥, 땅콩이 각각(各各) 39.32, 42.39, 28.07, 78.56mg/주(株) 이었고 질소(窒素)6kg/10a 시용구(施用區)에서는 각각(各各) 25.46, 24.97, 16.80, 47.65mg/주(株)로서 질소무시용구(窒素無施用區)가 질소(窒素)6kg/10a시용구(施用區)에 비(比)해 많아 균접종효과는 질소시용구(窒素施用區)보다는 무시용구(無施用區)에서 뚜렷했다.

• 미생물학회지
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• 제31권3호
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• pp.189-196
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• 1993

대두(Glycine max) 뿌리혹의 질소고정 공생균주 Bradyhizobium sp. SNU001 의 nod D 와 nodA 의 염기서열을 결정하였다. 총 314개의 아미노산을 암호화하는 nod D 의 open reading frame (ORF) 은 942bp 로 B. japonicum USDA110 의 nodD1 과 99.4% 의 유사성을 보여주었으며, 총 210개의 아미노산을 암호화하고 콩과식물의 Bradyrhizobium 에서는 처음으로 염기서열이 결정된 nodA 의 ORF 는 630bp 로 B. sp. (Parasponia) 의 nodA 와 81.5% 의 유사성을 보여주었다. nodYAB 오페론과 nodD 상류에서는 9bp의 반복서열을 각각 4번, 2번 가지는 보존적인 nodbox 가 발견되었으며 nodD 의 상류에서는 A, T-rich 서열도 존재하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제27권6호
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• pp.825-831
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• 2014

The aim of this study was to determine the nutritive value of some legume species in salt-affected soils of South-East Anatolian region using chemical composition and in vitro gas production kinetics. In this study, Lotus corniculatus, Trifolium alexandrinum, Medicago sativa were sown and tested in four different locations. A 3 by 4 factorial design with 3 legume species and 4 salt levels (non salty electrical conductivity (EC)<4 dS/m; low salt: 4 dS/m>EC<8 dS/m, medium saline: 8 dS/m>EC<16 dS/m and high salt: 16 dS/m>EC) was used in the study. Results indicated that salinity and plants had no significant effect on ash and ether extract. Dry matter (DM), acid detergent fiber, digestible dry matter, dry matter intake (DMI) were affected by plant, salinity and plant${\times}$salinity interaction. On the other hand neutral detergent fiber, relative feed value (RFV), and DMI were affected by salinity and plant${\times}$salinity interaction. Mineral contents were affected by plant species, salinity and salinity${\times}$plants interactions. In vitro gas production, their kinetics and estimated parameters such as were not affected by salinity whereas the gas production up to 48 h, organic matter digestibility, metabolizable energy (ME), and net energy lactation ($NE_L$) were affected by plant and plant${\times}$salt interaction. Generally RFVs of all species ranged from 120 to 210 and were quite satisfactory in salty conditions. Current results show that the feed value of Medicago sativa is higher compared to Lotus corniculatus and Trifolium alexandrinum.

• 한국응용곤충학회:학술대회논문집
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• 한국응용곤충학회 2003년도 추계 학술발표회
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• pp.151-151
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• 2003

• Asian-Australasian Journal of Animal Sciences
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• 제26권4호
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• pp.552-557
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• 2013

Partially purified ${\alpha}$-galactosidase from Bacillus sp. LX-1 was non-covalently immobilized on a reversibly soluble-insoluble polymer, Eudragit L-100, and an immobilization efficiency of 0.93 was obtained. The optimum pH of the free and immobilized enzyme was 6.5 to 7.0 and 7.0, respectively, while there was no change in optimum temperature between the free and immobilized ${\alpha}$-galactosidase. The immobilized ${\alpha}$-galactosidase was reutilized six times without significant loss in activity. The immobilized enzyme showed good storage stability at $37^{\circ}C$, retaining about 50% of its initial activity even after 18 d at this temperature, while the free enzyme was completely inactivated. The immobilization of ${\alpha}$-galactosidase from Bacillus sp. LX-1 on Eudragit L-100 may be a promising strategy for removal of ${\alpha}$-galacto-oligosaccharides such as raffinose and stachyose from soybean meal and other legume in feed industry.

• 식물병연구
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• 제19권2호
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• pp.77-83
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• 2013

Bean yellow mosaic virus (BYMV; genus Potyvirus, family Potyviridae) causes severe losses to various legume species and a number of non-legume species, particularly freesia plants. In a survey of virus diseases in Gyeonggi province, Korea, BYMV isolates were identified from many cultivated freesia species. Here, we determined the complete nucleotide sequences of a BYMV freesia isolate (BYMV-Fr; accession number FJ492961). BYMV-Fr genome consists of 9,545 nucleotides (nt) excluding the poly (A) tail and encodes 3,057 amino acid (aa), with an AUG start and UAG stop codon, containing one open reading frame typical of a potyvirus polyprotein. The polyprotein of BYMV-Fr was divided to ten proteins and the cleavage sites of each protein were determined. The coat protein (CP) and polyprotein of BYMV-Fr were compared at the aa level with those of the previously reported 4 BYMV isolates. BYMV-Fr shared 90.1 to 97.1 and 91.0 to 92.5% at the CP and polyprotein homology. Interestingly, BYMV-Fr showed identities of a lower level at the nt level of 5' noncoding region (61.4 to 67.6%) and at the aa level of P1 (71.4 to 72.8%), comparing with four BYMV isolates. Based on the aa sequence diversity of CP and polyprotein, phylogenetic analysis with the four BYMV isolates showed two distinct groups and BYMV-Fr and most BYMV isolates were most closely related to the clover yellow vein virus among 52 potyviruses. To our knowledge, this is the first report of the complete genome sequence of BYMV freesia strain.