• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.1
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• pp.7-12
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• 1994

This study was undertaken to evaluate the effect of legume reseeding on legume establishment, dry matter yield, quality and botanical composition of grasses in timothy(Ph1eum prarense L.) dominated pasture at alpine area. Establishment number and percentage of legume in timothy dominated pasture were increased with spring, red clover(Tr$olium pratense L.) and gramoxone $treatment Dry matter yield of pasture was increased with fall and grazing after overseeding, but, there waq no difference in legume species. Crude protein content(%) of grasses at the first cutting was increased with red clover and paraquat (gramoxone) treatment. Based on the results, it is suggested that spring in seeding time, red clover in legume species and oveneeding after gramoxone treatment are good for effective renovation of timothy dominated pasture at alpine area.

• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.3
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• pp.293-304
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• 1997

A field experiment was conducted at the forage experimental plots, Seoul National University, Suweon h m 1995 to 1996 to determine the effect of legume residues as a N source and N fertilizer on corn (Zea mays L.) silage yield, N uptake, and availability of inorganic N in the soil. Corn was grown following (i) red clover (Trifolium pratense L.), (ii) crimson clover (Trifolium incarnatum L.), (iii) alfalfa (Medicago sativa L.) and (iv) winter fallow. The plots were split into two rates of fertilizer N (0 and 90kg Nlha) in a split-plot experimental plan. Compared with fallow treatment, legumes depleted soil water in the surface 15cm at corn planting by 17 to 26%. As a result, corn emergence was markedly delayed with legume residues by 8 to 11 days. Corn silage DM yield was significantly reduced in the presence of legume residues by 2.0 to 3.4 and 1.5 to 2.5 tonlha compared with winter fallow treatment at 0 and 90kg fertilizer Nlha, respectively, but no significant difference in the corn DM yield was found between legume residue treatments. There was an overall tendency for increased corn yields with 90kg fertilizer Nlha compared to Okg fertilizer Nlha, although not all yield increases were significantly greater. The corn yield response to applied N suggests that a source of N from legume residues was not sufficient for a succeeding corn crop. There was significantly more N (P

• Journal of The Korean Society of Grassland and Forage Science
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• v.10 no.2
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• pp.110-114
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• 1990

Although corn is regarded as the most feasible forage corp, its relatively low content of protein is the critical we a kness for animal feeding. Many researches have been carried out to improve protein level in corn forage, however, there are no indicatable results but corn-legume intercropping. Plot test and proximate analysis were fullfill to compare dry matter yield and available nutrients of silage corn mono-culture system with those of corn-legume intercropping system of forage plant and silage. The MO culture system were observed by two stage of maturity, milk stage (Aug. 3), yellow stage (Aug. 24), and obtained following results. 1. Dry matter yields per 10 a at milk stage in corn mono-culture system was 596.2kg and corn-legume intercropping systems were 609.0 kg (corn-cowpea), 591.0 kg (corn-soybean) and 563.1 kg (corn-frenchbean), respectively. And comparable to them, 1508.9 kg (corn mono.), 1482.8 kg (corn-cowpea), 1482.6 kg (cornsoybean), 1379.1 kg (corn-frenchbean) were harvested at yellow stage. 2. The general trends of chemical composition by stages of maturity in corn mono-culture systems were higher than that of corn-legume intercropping system. 3. Crude protein content in corn-legume silages were significantly higher than corn mono-culture at yellow stage, except corn-frenchbean intercropping system. 4. Crude fiber content in corn-legume silage was higher than corn mono-culture silage harvested at yellow stage. 5. Consequently, corn-legume intercropping improved nutritional quality of silage than that of corn-monocultivated silage.

• Korean Journal of Food Science and Technology
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• v.20 no.1
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• pp.72-78
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• 1988

Microstructures of 18 kinds of legume seeds (15 varieties including 3 strains) which were cultivated in tropical areas, were observed under a light microscope. Majority of legume seeds were composed of starchy cotyledonary cells in which large amounts of single starch granules were contained, while a few had cotyledonary cells filled with a number of protain bodies. Starch granules were different in size and shape depending on varieties. Some contained lipid bodies distributed in cytoplasmic network, and were distinctive in thick cell walls. Microstructure of soybean was also observed for the comparison of the structures.

• Korean Journal of Food Science and Technology
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• v.49 no.6
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• pp.638-643
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• 2017

In this study, the approximate compositions, color properties, texture properties, physical characteristics, and sensory evaluation of muffins prepared from legumes (mung bean, cowpea, chickpea, or lentil) and wheat flour blends was evaluated. Pasting viscosity of legume flours was lower than that of wheat flour. The moisture, crude ash, and crude protein content of muffins made with legume-wheat flour blends were higher than those of control muffins made exclusively with wheat flour. The lightness value of muffins was decreased by adding legume flour. The hardness of muffins made from legume-wheat flour blends was higher than that of control, whereas springiness and cohesiveness was lower. The weight of muffins made with legume-wheat flour blends were higher than that of control, whereas the height and volume were lower than those of control. The scores of taste, aroma, and texture of muffins were not significantly changed by adding legume flours. The overall acceptability of muffins that were made with lentil or chickpea flour was similar to that of control muffins.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.4
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• pp.517-522
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• 2007

The aim of this study was to compare in vitro gas production kinetics, metabolizable energy (ME), organic matter digestibility (OMD) and microbial protein (MP) production of widely used legume hays in ruminant nutrition in Turkey. Gas production were determined at 0, 3, 6, 12, 24, 48, 72 and 96 h and their kinetics were described using the equation p = a+b ($1-e^{-ct}$). There were significant differences among legume hays in terms of chemical composition. The crude protein content of legume hays ranged from 11.7 to 18.6% of dry matter (DM); crude fat from 2.1 to 3.5% DM; neutral detergent fiber from 35.6 to 52.0% DM; acid detergent fiber from 32.0 to 35.5% DM and acid detergent lignin 1.7 to 11.0% DM. Total gas production after 96 h incubation ranged between 61.67 and 76.00 ml/0.200 g of substrate. At 24, 72 and 96 h incubation the total gas production for common vetch were significantly (p<0.01) higher than those of the other legume hays. The ME, OMD and MP of legume hays ranged from 9.09 to 11.12 MJ/kg DM, 61.30 to 75.54% and 90.35 to 138.05 g/kg DM, respectively. The ME, OMD and MP of common vetch was significantly (p<0.01) higher than those of the other hays due to low cell-wall contents and high crude protein. At the end of the experiment, differences in chemical composition of legume hays resulted in the differences in the in vitro gas production, gas production kinetics and the estimated parameters such as ME, OMD and MP. Common vetch can be recommended to hay producers and ruminant breeders, due to high ME, OMD and MP production.

• Asian-Australasian Journal of Animal Sciences
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• v.8 no.2
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• pp.107-111
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• 1995

An experiment was conducted at Pabna milkshed area under Bathan condition during November, 1992 to February 1993 with 16 milking cows fed legume forage with straw supplementation (treated group) and 8 milking cows fed legume forage only (control group). From the study, the results revealed that supplementation of straw with leguminous diet increases the total dry matter intake (DMI) of 11.83 kg/d/cow for the treated group and 11.53 kg/d/cow for the control group. The average daily legume forage intake was $37.39{\pm}8.67kg/d/cow$ and $49.62{\pm}10.57kg/d/cow$ for the treated and control group respectively and the difference was significant (p < 0.05). The results also exhibited that due to the supplementation of straw, the legume forage intake reduced by 12 kg/d/cow. The forage dry matter intake (DMI) kg/d/cow for the treated and control groups were $6.18{\pm}1.44kg$ and $8.38{\pm}1.95kg$ respectively. The milk production was $8.64{\pm}1.15litre/day$ for the treated group which was significantly (p < 0.05) higher than the control group ($7.74{\pm}1.24litre/day$). The average initial body weight of the supplemented and control groups were $338.06{\pm}39.32kg$ and $329.87{\pm}48.03kg$ respectively. Whereas, the final body weight of supplemented group was $344.33{\pm}35.90kg$ and control group was $330.35{\pm}37.28kg$. It may be concluded that straw supplementation with legumes diet could save legume forage for further use as well as increase milk production.

• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.3
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• pp.153-160
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• 1992

This experiment was conducted to investigate the adaptability of legume in different legume-grass mixtures according to altitude(600, 800, lOOOm a.s.1.) in the alpine area. The experimental plot was allocated as a split plot design with three replications. The main plots were sowing season(spring and fall) and subplots legume-grass mixture(alfa1fa-grass, red clover-grass and white clover-grass mixture). The results obtained are summarized as follows: 1. At 600m, in case of sowing alfalfa, red clover and white clover in fall. each legume of legume-grass mixtures was consistuted about 30% without between-year variation and then all legumes at fall sowing could grow. 2. At 800m, in case of sowing white clover in spring this species was consistuted about 14.7 % at the last cutting without severe between-year variation. 3. At 1000m, only white clover sown in spring like at 800m was somewhat maintained without severe between-year variation. 4. Percentage and DM yield of legume considerably decreased with the rise of altitude and alfalfa couldn't grow more than 800m.

• Journal of Microbiology and Biotechnology
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• v.23 no.6
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• pp.856-863
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• 2013

Application of rhizospheric fungi is an effective and environmentally friendly method of improving plant growth and controlling many plant diseases. The current study was aimed to identify phytohormone-producing fungi from soil, to understand their roles in sesame plant growth, and to control Fusarium disease. Three predominant fungi (PNF1, PNF2, and PNF3) isolated from the rhizospheric soil of peanut plants were screened for their growth-promoting efficiency on sesame seedlings. Among these isolates, PNF2 significantly increased the shoot length and fresh weight of seedlings compared with controls. Analysis of the fungal culture filtrate showed a higher concentration of indole acetic acid in PNF2 than in the other isolates. PNF2 was identified as Penicillium sp. on the basis of phylogenetic analysis of ITS sequence similarity. The in vitro biocontrol activity of Penicillium sp. against Fusarium sp. was exhibited by a 49% inhibition of mycelial growth in a dual culture bioassay and by hyphal injuries as observed by scanning electron microscopy. In addition, greenhouse experiments revealed that Fusarium inhibited growth in sesame plants by damaging lipid membranes and reducing protein content. Co-cultivation with Penicillium sp. mitigated Fusarium-induced oxidative stress in sesame plants by limiting membrane lipid peroxidation, and by increasing the protein concentration, levels of antioxidants such as total polyphenols, and peroxidase and polyphenoloxidase activities. Thus, our findings suggest that Penicillium sp. is a potent plant growth-promoting fungus that has the ability to ameliorate damage caused by Fusarium infection in sesame cultivation.

• The Plant Pathology Journal
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• v.17 no.2
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• pp.67-70
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• 2001

Medicago truncatula is a diploid legume plant related to the forage crop alfalfa. Recently, it has been chosen as a model species for genomic studies due to its small genome, self-fertility, short generation time, and high transformation efficiency. M. truncatula engages in symbiosis with nitrogen-fixing soil bacterium Rhizobium meliloti. M. truncatula mutants that are defective in nodulation and developmental processes have been generated. Some of these mutants exhibited altered phenotypes in symbiotic responses such as root hair deformation, expression of nodulin genes, and calcium spiking. Thus, the genes controlling these traits are likely to encode functions that are required for Nod-factor signal transduction pathways. To facilitate genome analysis and map-based cloning of symbiotic genes, a bacterial artificial chromosome library was constructed. An efficient polymerase chain reaction-based screening of the library was devised to fasten physical mapping of specific genomic regions. As a genomics approach, comparative mapping revealed high levels of macro- and microsynteny between M. truncatula and other legume genomes. Expressed sequence tags and microarray profiles reflecting the genetic and biochemical events associated with the development and environmental interactions of M. truncatula are assembled in the databases. Together, these genomics programs will help enrich our understanding of the legume biology.