• Title/Summary/Keyword: Lee Ja Hyeon

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Development of Leaf Mutant Cultivars of Cymbidium goeringii by Ethyl-methane-sulfonate (EMS) Treatment. (Ethyl-methane-sulfonate(EMS) 처리에 의한 춘란 잎 돌연변이 품종의 개발)

  • Shin, Yun-Ho;Song, In-Ja;Kang, Eun-Jung;Bae, Tae-Woong;Sun, Hyeon-Jin;Kang, Si-Young;Lim, Pyung-Ok;Lee, Hyo-Yeon
    • Korean Journal of Plant Resources
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    • v.24 no.1
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    • pp.17-22
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    • 2011
  • This study was for developing leaf chlorophyll mutant cultivars of Cymbidium goeringii by ethyl-methanesulfonate(EMS) treatment. Chlorophyll mutant rhizomes were easily produced by 0.2% EMS treatment in this genus. Among the mutants, they became dark brown about 50% of the rhizomes. When the dark-brown rhizomes were cultured in a solidified MS medium, new rhizomes were formed from part of the old ones. Chlorophyll mutant rhizomes were obtained from subcultured meristem tissues of newly-formed rhizomes. The rhizomes were cut and subcultured for a year and then became mutant plants. As the results, they produced 4 kinds of leaf mutant cultivars; zigzag-striped, comb-striped, net-striped, and dwarf types, indicating that the EMS treatment in the rhizome could produce versatile leaf chlorophyll regulating mutants. These results suggest that our method is useful for developing leaf mutant cultivars of this planta which they are estimated as higher commercial values.

A Flora of Vascular Plants in Mt. Cheongnyangsan (Bonghwa-gun, Andong-si) (청량산(봉화군, 안동시)의 관속식물상)

  • Nam, Bo Mi;Kim, Jae Young;Jeong, Seon;Lee, Jae-Hyeon;Nam, Myoung Ja;Oh, Byoung-Un;Chung, Gyu Young
    • Korean Journal of Plant Resources
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    • v.28 no.5
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    • pp.616-634
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    • 2015
  • This study was carried out to elucidate the distribution of vascular plants and their usefulness of Mt. Cheongnyangsan (870 m) in Gyeongsangbuk-do. The vascular plants that were collected 16 times 2006 and 2014 consisted a total of 614 taxa; 97 families, 330 genera, 541 species, 3 subspecies, 61 varieties and 9 forms. For the Korean endemic plants, 21 taxa were recorded and 8 taxa of Vulnerable Species (VU) and 5 taxa of Least Concerned species (LC) categorized by the Korean Forest Service as rare plants were investigated in this region. Furthermore, Ⅴ, Ⅳ, Ⅲ degrees of floristic regional indicator plants designated by the Korean Ministry of Environment included 2 taxa, 10 taxa and 18 taxa, respectively. Moreover, four species of distributional interesting plants, Aconitum austro-koreense Koidz., Allium spirale Willd., Dipsacus japonicus Miq. and Pinellia tripartita (Blume) Schott, in Mt. Cheongnyangsan were discovered by this study. Among them, edible, pasturing, medicinal, ornamental, timber, dye, fiber and unknown usefulness plants included 234 taxa, 213 taxa, 174 taxa, 62 taxa, 16 taxa, 12 taxa, 11 taxa and 167 taxa, respectively. In addition, 32 taxa of naturalized plants were observed.

제초제저항성 GM 들잔디(JG21)의 포장시험을 통한 생물학적 안전성평가

  • Bae, Tae-Ung;Gang, Hong-Gyu;Jeong, Ok-Cheol;Song, In-Ja;Seon, Hyeon-Jin;Go, Seok-Min;Im, Pyeong-Ok;Song, Pil-Sun;Lee, Hyo-Yeon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2010.10a
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    • pp.3-3
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    • 2010
  • GM 들잔디의 환경위해성평가는 유전자변형생물체의 국가간이동등에 관한 법률 (2008년)에 안전관리 및 시험평가가 수행되었다. 일반적으로 GM 작물의 환경위해성평가는 도입 유전자의 안정적 발현여부, 일반적으로 사용되어온 식물과의 실질적 동등성, 화분비산에 의한 유전자이동성 검정, GM재배작물 주변 환경에 미치는 영향, GM작물 및 유전자의 인체 및 동물에 대한 안전성 평가, 위해성 관리 등이 있다. 본 발표에서는 제초제저항성 GM 들잔디를 실용화 하기위한 환경위해성평가의 내용을 소개하기 위하여 GM 들잔디의 안전성평가를 수행한 포장시험에 대해서 간략히 발표하고자 한다. GM 들잔디는 제초제저항성 유전자가 식물체내에 1개의 복제수를 갖으며, 후세대에서도 안정적으로 발현되었다. 또한 TAIL PCR을 통한 도입 유전자의 삽입 부위를 확인하였고, 주변염기서열을 이용한 JG21 들잔디 만의 특이적 염기서열을 얻었다. GM 들잔디의 실질적 동등성검정은 격리온실 수준과 격리포장 수준에 생물학적, 화학적 특성을 조사하였고, 일반 들잔디와 큰 차이가 없음을 확인하였다. GM 들잔디의 화분은 일광조건하에서 약 1시간 이내에 불활성 되었고, 실내의 실온조건에서는 3시간까지 활성이 유지되었다. GM 들잔디 재배포장 ($12m{\times}6m$ 크기)으로부터 화분비산 밀도는 3 m 이내에서 높게 나타났고, 9 m 이상의 거리에서는 낮은 빈도로 일정하게 나타났다. 화분비산에 의한 GM 들잔디 유전자이동성은 근접거리에서 약 15%가 관찰되었고, 반경 3 m 이내에서 거리에 따라서 2 ~ 0.12%의 유전자이동성이 관찰되었다. 각각의 시험모형에 따른 GM 들잔디와 일반들잔디 간의 교잡율을 조사한 결과 들잔디 교잡율은 GM 잔디의 면적과 거리에 의존적이었고, GM 들잔디 면적에 따라서 6-21%의 교잡율이 나타났다. GM들잔디의 인체위해성평가는 독성 및 알레르기 반응성을 검정하였고, 도입유전자 산물인 PAT 단백질은 독성 및 알레르기 반응성이 없음이 알려져 있다. 본 연구에서는 GM들잔디의 독성 및 알레르기 반응성을 검토한 결과 아플라톡신 등의 독성은 검출되지 않았으나, GM과 일반 들잔디 모두에서 화분 알레르기 반응성이 양성으로 나왔다. 그러나 GM들잔디에 도입된 유전자에 의한 알레르기 반응이 아닌 일반들잔디 화분에 가진 알레르겐에 의한 반응성이라 할 수 있다. GM들잔디의 비의도적 방출가능성을 조사하기위하여 시험포장외부의 자연환경에 대해서 환경모니터링을 수행한 결과 GM 들잔디의 유출 및 유전자이동 의한 제초제저항성 들잔디는 나타나지 않았다.

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Effects of Oral Health Education Program on the Oral Health Knowledge, Oral Health Behavior and Oral Hygiene Status of Elementary School Students (구강보건교육 프로그램이 초등학생의 구강보건지식, 구강보건행위 및 구강위생 상태에 미치는 효과)

  • Son, Mi-Hyang
    • Research in Community and Public Health Nursing
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    • v.14 no.1
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    • pp.24-35
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    • 2003
  • The purpose of this study was to evaluate the effects of oral health education program on the oral health knowledge, oral health behavior and oral hygiene status of elementary school students. The design of this study is nonequivalent control group pretest-posttest gesign. Data was collected between the 17th of June and 13th of July in 2002, and the Experimental group were 131 students of Y Elementary School. which was one of the two elementary schools in K City, Gyeongsangbuk-do, and the control group was 140 students of C Elementary School, which was similar to Y Elementary School in geographical and economical properties and size. As for research tools, the researcher developed tools of measuring oral health knowledge and oral health behavior by modifying the tools developed by Hye-Kyong Kim (2001), In-hyang Seo (1988), Ho-Youn Lee (2001), Hyeon-ja Jeon (1998). To measure oral hygiene status. the researcher applied a coloring agent to the surface of the teeth after brushing, rinsed the mouth, and calculated colored plaque on the surface of the teeth, which was recorded on a sheet and analyzed using the plaque index. The data analysis was done using frequency, percentage, mean, standard deviation, Chisquare test, t-test, ANCOVA, Two-way ANOVA, Simple main effects, One-way ANOVA, and Duncan. Using with SPSS WIN 10.0. The result of this study are summarized as follows: 1. The hypothesis was supported 'The elementary school students which was given oral health education program will shows higher oral health knowledge, oral health behavior, oral hygiene status then control group' 2. According to the result of verifying the hypothesis, 'The effect of oral health education program will be different between junior and senior' the hypothesis was partly supported To summarize result of this research, the experimental group, to which oral health education was given, was improved in oral health knowledge, oral health behavior and oral hygiene status compared to the control group, so the oral health education program was effective for the oral health of elementary school students.

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Effects of Oenanthe javanica, Coicis lachryma-jobi L. var., and Plantaginis asiatica L. Water Extracts on Activities of Key Enzymes on Lipid Metabolism (미나리 줄기(Oenanthe javanica), 율무(Coicis lachryma-jobi L. var.), 차전자(Plantaginis asiatica L.) 물 추출물이 지질대사)

  • Lee, Hyeon-Ju;Chung, Mi-Ja;Kim, Dae-Jung;Choe, Myeon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.11
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    • pp.1516-1521
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    • 2009
  • This study was carried out to estimate beneficial effects of medicinal plant [Oenanthe javanica (MNR), Coicis lachryma-jobi L. var. (YM), Plantaginis asiatica L. (CJJ)] water extracts on activities of key enzymes such as lipoprotein lipase (LPL), acyl-CoA synthetase (ACS) and carnitine acetyltransferase (CAT) on lipid metabolism. LPL and ACS were extracted from the epididymal adipose tissue and liver of Zucker lean rats (lean) and Zucker fatty rats (fa/fa). MNR or YM water extract treatment significantly reduced the activity of lean and fa/fa LPL. When 10000 ppm of MNR, YM, and CJJ water extracts were tested, they decreased fa/fa LPL activity by 32.5%, 30.1% and 22.8%, respectively. The lean ACS activity was significantly higher in YM water extract treatment compared to the control and the MNR water extract treatment significantly increased the activity of fa/fa ACS, compared to the activity in the control (p<0.05). MNR water extract activated fa/fa ACS activity by 12-fold compared with control at 10000 ppm concentration. CAT activity was significantly higher in 10000 ppm and 20000 ppm CJJ water extract treatment than in the control. Thus, the MNR, YM and CJJ water extracts may have beneficial effects due to activities of enzymes related with fat metabolism in obese humans.

Protective Effect of Dopaol β-D-glucoside Isolated from East Asian Monk'shood on Cisplatin-Induced Nephrotoxicity (한라돌쩌귀로부터 분리된 Dopaol β-D-glucoside의 신장독성 보호효과)

  • Nho, Jong Hyun;Jung, Ja Kyun;Jung, Ho Kyung;Jang, Ji Hun;Jung, Da Eun;Lee, Ki Ho;Kim, A Hyeon;Sung, Tae Kyoung;Park, Ho;Cho, Hyun Woo
    • Korean Journal of Medicinal Crop Science
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    • v.25 no.4
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    • pp.231-237
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    • 2017
  • Background: Cisplatin is one of the most extensively used chemotherapeutic agents for the treatment of cancer, including bladder, and ovarian cancers. However, it has been shown to induce nephrotoxicity, despite being an outstanding anti-cancer drug. In this study, we investigated the protective effect of dopaol ${\beta}$-D-glucoside (dopaol) on cisplatin-induced nephrotoxicity. Methods and Results: To confirm the protective effect of dopaol on cisplatin-induced nephrotoxicity, HK-2 cells were treated with $20{\mu}M$ cisplatin and $80{\mu}M$ dopaol. Cisplatin increased apoptosis, caspase-3 activity and mitochondrial dysfunction; however pretreatment with $80{\mu}M$ dopaol successfully attenuated apoptosis, caspase-3 activity and mitochondrial dysfunction. To evaluate the protective effect dopaol on cisplatin-induced nephrotoxicity in vivo, we used an animal model (balb/c mice, 20 mg/kg, i.p. once/day for 3 day). The results were similar to those obtained using HK-2 cells; renal tubular damage and neutrophilia induced by cisplatin reduced following dopaol injection (10 mg/kg, i.p. once/day for 3 day). Conclusions: These results indicate that dopaol treatment reduced cisplatin-induced nephrotoxicity in vitro and in vivo, and can be used to treat cisplatin-induced nephrotoxicity. However, further studies are required to determine the toxicity high dose dopaol and the signal pathways involved in its mechanism of action in animal models.

Fabrication of Inductors, Capacitors and LC Hybrid Devices using Oxides Thin Films (산화물 박막을 이용한 인덕터, 캐패시터 및 LC 복합 소자 제조)

  • Kim, Min-Hong;Yeo, Hwan-Guk;Hwang, Gi-Hyeon;Lee, Dae-Hyeong;Kim, In-Tae;Yun, Ui-Jun;Kim, Hyeong-Jun;Park, Sun-Ja
    • Korean Journal of Materials Research
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    • v.7 no.3
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    • pp.175-179
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    • 1997
  • bliniaturization oi microwave circuit components is an important issue with the development in the mobile communication. Capacitors, inductors anti hybrid devices of these are building blocks of electric circuits, and the fabrication of these devices using thin film technology will influence on the miniaturization of electronic devices In this paper, we report the successful fabrication of the inductors, capacitors and LC hybrid devices using a ferroelectric and a ferromagnetic oxide thin iilm. Au, stable at high temperatures in oxidizing ambient, is patterned by lift-off process, and oxide thin films are deposited by ion beam sputtering and chemical vapor deposition. These devices are characterized by a network analyzer in 0.5-15GtIz range We got the inductance of 5nH, capacitance oi 10, 000 pF and resonant frequencies of $10^{6}-10^{9}Hz$.

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Quality Properties of Commercial Chungkukjang in Korea (국내 시판 청국장의 품질 및 특성)

  • Ko, Yu-Jin;Son, Yong-Hwi;Kim, Eun-Ja;Seol, Hui-Gyeong;Lee, Gyeong-Ran;Kim, Dong-Hyeon;Ryu, Chung-Ho
    • Journal of agriculture & life science
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    • v.46 no.1
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    • pp.177-187
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    • 2012
  • This study aimed to improve the quality with acomparison of quality characteristics by collecting Chungkukjang in the market, and to establish the foundation data for starter, which support to manufacture standardized Chungkukjang products. Firstly, Chungkukjang was collected from the industrial company and home-made production which located in the several regions of Korea. Moisture contents of collected Chungkukjang were ranged from 56.3 to 62.1% and salinity was 0.23 to 11.51%. Total nitrogen and amino nitrogen contents of Chungkukjang ranged from 5.82 to 8.76% and 173.71 to 2,700.66 mg%, respectively. Nitrogen degradation ratio, which means the proportion of amino nitrogen to total nitrogen, was 2.88~26.93%. Collected Chungkukjang AG and Z have predominant value in histamine and tyramine contents as 755.40 and 1913.51 mg/kg, respectively, and viable cells count in collected Chungkukjang, maximum was $9.6{\times}10^9CFU/g$. As a result, most products showed various properties in flavor and nutrition, and it is supposed to be caused on use of different soybean species and unstandardized fermentation process.

Effect of Ankle Stabilization Exercise with Kinesio Taping on Static·Dynamic Balance (키네시오 테이핑을 적용한 발목 안정화운동이 정적·동적 균형에 미치는 효과)

  • Su-Jin Hong;Na-Young Kim;Sun-Ha Kim;Sung-Yeon Park;Yeon-jung Lee;Ye-Won Jeon;Seung-Yeon Jung;Jin-Young Jeong;Hyeon-Jeong Jo;Jeong-Ja Kim
    • Journal of Korean Clinical Health Science
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    • v.11 no.1
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    • pp.1644-1653
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    • 2023
  • Purpose: The purpose of this study was to investigate the effect of kinesio taping application on static and dynamic balance during ankle stabilization exercise. Method: H University in Gunsan is recruiting subjects with unstable ankles (N=12). The 12 subjects were randomly divided into groups (n=6) that performed ankle stabilization exercises by applying kinesio taping and groups that performed ankle stabilization exercises only (N=6). Exercise was done twice a week for 4 weeks. All groups conducted the same exercise program, including stretching, for 40 minutes. The exercise program was conducted in the following order. It was conducted in the order of 5 minutes of stretching, 30 minutes of exercise program, and 5 minutes of finishing stretching. To measure the change in static and dynamic balance, the experimenter and control group measured the change by conducting the Cumberland ankle instability tool, the Y-balance test, and the Stork balance standing test (SBST). Results: There was a statistically significant difference in static and dynamic balance between the group with kinesio taping (experimental group) and the group without kinesio taping (control group) in patients with chronic ankle instability. However, there was no statistically significant difference in static and dynamic balance before and after intervention between groups. Conclusion: These results were expected to help improve dynamic and static balance in ankle instability when applying kinesio taping and balance exercises, but there was no significant difference between the experimental group and the control group because the experiment period was short.

Process Optimization for the Industrialization of Transparent Conducting Film (투명 전도막의 산업화를 위한 공정 최적화)

  • Nam, Hyeon-bin;Choi, Yo-seok;Kim, In-su;Kim, Gyung-jun;Park, Seong-su;Lee, Ja Hyun
    • Industry Promotion Research
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    • v.9 no.1
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    • pp.21-29
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    • 2024
  • In the rapidly advancing information society, electronic devices, including smartphones and tablets, are increasingly digitized and equipped with high-performance features such as flexible displays. This study focused on optimizing the manufacturing process for Transparent Conductive Films (TCF) by using the cost-effective conductive polymer PEDOT and transparent substrate PET as alternatives to expensive materials in flexible display technology. The variables considered are production speed (m/min), coating maximum temperature (℃), and PEDOT supply speed (rpm), with surface resistivity (Ω/□) as the response parameter, using Response Surface Methodology (RSM). Optimization results indicate the ideal conditions for production: a speed of 22.16 m/min, coating temperature of 125.28℃, and PEDOT supply at 522.79 rpm. Statistical analysis validates the reliability of the results (F value: 18.37, P-value: < 0.0001, R2: 0.9430). Under optimal conditions, the predicted surface resistivity is 145.75 Ω/□, closely aligned with the experimental value of 142.97 Ω/□. Applying these findings to mass production processes is expected to enhance production yields and decrease defect rates compared to current practices. This research provides valuable insights for the advancement of flexible display manufacturing.