• Journal of Forest and Environmental Science
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• v.27 no.1
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• pp.33-37
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• 2011

This study was carried out to establish a proper cultivation site and diagnose the drought-tolerance of Allium ochotense leaves and Allium microdictyon leaves by using pressure - volume curve method. The Allium microdictyon leaves had more lower osmotic water potential ${\psi}osat$, and osmotic water potential at turgor loss point ${\psi}otlp$ than Allium ochotense leaves. Then, the Allium microdictyon leaves was slightly higher drought-tolerance than Allium ochotense leaves. Therefore, We suggest that growth of the two Allium species was appropriate for relative moisture forest.

• Food Science and Preservation
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• v.11 no.2
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• pp.175-181
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• 2004

As a foundational study for notifing excellence of persimmon leaves tea, the chemical component and antioxidant activity were investigated in persimmon leaves from Dungsi, Gabjubaekmok, Weulhasi and Cheongdobansi and green tea leaves. Total sugar contents in all persimmon leaves more higher than that of green tea leaves, and the highest free sugar contained in persimmon and green tea leaves was sucrose. Free sugars present in persimmon and green tea leaves were composed of sucrose, glucose, fructose, maltose and xylose. Sucrose and fructose took more than 70% of total sugar contents. 31∼32 kinds of amino acid were detected in persimmon leaves and 35 kinds in green tea leaves. And total amino acids contained in persimmon leaves were Dungsi, Gabjubaekmok, Weulhasi and Cheongdobansi, respectively 60.40 nmol/${\mu}$L, 53.21 nmol/${\mu}$L, 52.29 nmol/${\mu}$L and 47.58 nmol/${\mu}$L. Total amino acid contents in green tea leaves was the most abundant of all as 114.72 nmol/${\mu}$L. The contents of vitamin C in persimmon and green tea leaves were in the range of 0.015∼0.089% and 0.01%, respectively. Vitamin C was significant higher content in the persimmon leaves than in green tea leaves. Caffeine was not detected in all persimmon leaves, but the caffeine content of green tea leaves was 6.63 mg/l00 g. The content of catechin was showed in the orders of Cheongdobansi, Gabjubaekmok, Weulhasi, Dungsi and green tea leaves; 0.35%, 0.34%, 0.24%, 0.18% and 0.07%, respectively. The contents of gallic acid in Dungsi and Gabjubaekmok were 0.32% and 0.20%. That of green tea was 1.41%, it was the highest content in all samples. The content of calcium in Chengdobansi was most abundant in all samples as 3516.14 ppm, it was 4∼5 times as that of green tea leaves. Flavor component pattern among persimmon leaves was similar, but that of green tea leaves was different. The IC50(${\mu}$g) value of Dungsi, Weulhasi, Gabjubaekmok, Cheongdobansi and green tea were 64.5, 42.0, 47.0, 64.0 and 19.0 respectively.

• Journal of the Korean Society of Tobacco Science
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• v.9 no.1
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• pp.19-26
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• 1987

KA 101 and Xanthi-Basma were cultivated in Korea and Greece to compare the respective responses to environments on their leaf composition during 1984 and 1985. Cured leaves cultivated in Greece had brighter and greenish color with 2-4 times higher total chlorophyll and carotenoids showing higher contents of total nitrogen, nicotine, petroleum ether extract, volatile acids and neutrals, but crude ash and pH of leaves at top stalk position were lower than in Korean cultured leaves. Xanthi-Basma showed higher contents of nicotine and total nitrogen, but lower contents of reducing sugar than KA 101. There were no difference in crude ash, petroleum ether extract contents and pH of leaves. Among stalk positions, the upper stalk leaves in Greece had higher contents of total nitrogen, protein nitrogen, nicotine, petroleum ether extract and volatile acids but lower contents of reducing sugar than lower ones., whereas the supper stalk leaves in Korea had much higher contents of total nitrogen, protein nitrogen, pH, crude ash, but not higher contents of petroleum ether extract and volatile acid contents than lower stalk position. It suggest that in chemical criteria the better quality leaves are on the upper stalk position in Greece but not the same in Korea probably due to the rainy weather conditions during the later growth stage in Korea.

• Journal of the Korean Society of Tobacco Science
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• v.13 no.2
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• pp.66-70
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• 1991

A field experiment was conducted to study the effects of fertilizer application rate and number of harvested leaves per plant on selected agronomic, chemical and physical characteristics of burley tobacco(Nicotiana tabacum L. cv Burley 21) in 1988, 1989 and 1990 at Chonju Experiment Station, Korea Ginseng SE Tobacco Research Institute. 175.0 or 227.5 kilogram per 10a of compound fertilizer(N-P2O5-K2O= 10-10-20) were applied as main plot, and 2 or 4 of upper leaves was topped off with 0, 2 or 4 of the lower leaves removed as sub plot. Yield, total alkaloid and total nitrogen content of cured leaves were high in the higher level of compound fertilizer plot, and increasing the level of compound fertilizer had an adverse effect on physical characteristics. Reducing the number of harvested leaves had a negative effect on yield regardless of whether the leaf number was reduced by lower topping or removing the bottom leaves, but increased the rate of higher grades leaves. Differences in total alkaloid, total nitrogen and some physical properties among number of harvested leaf per plant were smaller than expected.

• Archives of Pharmacal Research
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• v.27 no.11
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• pp.1114-1119
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• 2004

This paper describes the quantitative determination of flavonoids, tannins and ellagic acid in the leaves from wild and cultivated variations of Rubus L. species (Rosaceae): raspberry (2 wild and 13 cultivars) and blackberry (3 wild and 3 cultivars). The content of flavonoids was analyzed using spectrophotometric (the Christ-M llers method) and HPLC analysis after acid hydrolysis. The content of tannins was determined by the weight method, with hide powder, described by German Pharmacopoeia 10 (DAB 10). Ellagic acid content was examined using the HPLC method after acid hydrolysis. Flavonoid content, determined using the Christ-Muller's method was higher for the blackberry leaves than for the raspberry leaves and varied between 0.46% and 1.05%. Quercetin and kaempferol were predominant in all samples analyzed using the HPLC method. The highest flavonoid content was found in the leaves of R. nessensis (1.06%); with results in all of the examined samples varying between 0.27% and 1.06%. The concentration of ellagic acid in all species was determined after acid hydrolysis and ranged from 2.06% to 6.89%. The leaves of raspberries are characterized by greater amounts of tannins (varying between 2.62% and 6.87%) than the leaves of other species. The results from this study indicate that the analyzed species are a rich source of flavonoids, ellagic acid and tannins, which may be used for the quality assessment of Rubus L. species leaves.

• Journal of the Korean Society of Tobacco Science
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• v.22 no.2
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• pp.107-113
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• 2000

This study was carried out to investigate the changes in composition of the non-volatile organic acid, fatty acid and polyphenolic compounds during air-curing in burley tobacco leaves, and the effect of curing methods on the contents in air-cured leaves. The air-cured variety, (Nicotiana tabacum cv KB108) was normally grown at the Chonju tobacco experiment station in 1998. Plants designated for the each curing methods were harvested on the same date, and the ripe leaves for primed curing were harvested at the tenth leaf position from the top on the stalk. The major compounds of non-volatile organic acid and fatty acid were malic, citric, oxalic, palmitic, and linolenic acid. The concentrations of malic acid, unsaturated fatty acids, chlorogenic acid and rutin in cured leaves were remarkably decreased during curing, while citric acid was increased. The changes of these compounds showed the similar pattern during both primed and stalk curing. In connection with curing methods, the contents of malic, linoleic and linolenic acid were higher in excessive cured leaves than those in primed cured or stalk cured leaves, while the content of citric acid was lower in excessive cured leaves than that in primed cured or stalk cured leaves.

• Journal of the Korean Society of Tobacco Science
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• v.22 no.2
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• pp.143-150
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• 2000

This study was carried out to investigate the effect of storage period on the physico-chemical properties of tobacco leaves expanded by $CO_2$. The flue-cured and burley tobacco leaves produced from 1996 to 1999 were processed, and stored for that year or 3 years in processing plant. As compared with 1996 crop, the expanded rate of flue-cured tobacco leaves in 1999 crop was higher approximately 18%, while that of burley was higher about 10%. The filling capacity of cigarettes was decreased as storage time became shorter, but the combustibility was improved. The rate of large particles (over 3.36mm) of expanded tobacco showed decreasing tendency as the storage period became longer. The change rate of chemical contents in tobacco leaves between before and after expansion had no significant difference among corp years. The tar and CO contents of cigarettes smoke were reduced in the case of flue-cured tobacco leaves as storage time became shorter, while they were slightly increased in the case of burley tobacco leaves. The ammonia content of burley leaves was decreased remarkably in 1996 and 1999 crop.

• Journal of the Korean Society of Tobacco Science
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• v.22 no.2
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• pp.138-142
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• 2000

The effects of stalk cutting time and environmental factors such as air temperature, leaf temperature, solar radiation and leaf moisture content during harvesting and curing in burley tobacco(Nicotiana tabacum L.) on weight loss of fresh stalks and sunburning in leaves were investigated at Chonju Experiment Station, Korea Ginseng & Tobacco Research Institute in 1996 and 1997. Twelve to fifteen percent of the fresh weight was lost in 3 to 4 hours after stalk-cutting, and sunburned leaves could be observed in case of stalk cutting between 11:00 and 15:00 O'clock on a clear sunny day, when the air temperature was 34 to 35$^{\circ}C$, leaf temperature 52 to 54$^{\circ}C$, and solar radiation 700 to 940 w/$m^2$. The leaves exposed to this weather condition were sunburned within 1 hour after stalk cutting. But low temperature (below $25^{\circ}C$) with high solar radiation(above 700w/m2) or high temperature(above 3$0^{\circ}C$) with low solar radiation (below 600w$m^2$) did not induce the sunburn damage in leaves. As the leaf temperature and leaf moisture content were higher, the sunburned leaves increased. The leaves at the higher stalk position were more easily sunburned than those at the lower. This result indicates that the immature leaves with higher chlorophyll content might be more susceptible to sunburning.

• Journal of the Korean Society of Tobacco Science
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• v.21 no.1
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• pp.5-9
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• 1999

In recent years, the line of increase in policy on tobacco production triggered a rise in the loading volume per bulk curing barn, and manufacturers boosted the output of their blowers in order to prevent dirty leaves in the process of curing. for this reason, we studied the effect of the reducing air flow in bulk curing chamber from the color fixing stage to the stem drying stage of flue curing process on physical properties of cured leaves. The control of air flow was composed of the reducing air velocity of blower by means of a voltage regulator (slidac), and condition before color fixing stage was all the same with the conventional curing method. As a result, an effectiveness of improvement in the physical properties of cured leaves were observed. The leaves cured by this method were somewhat orange in color of upper stalk position, better bodied, and less brittle compared with the leaves produced by conventional curing. However, the leaves cured by this method had a little sharpness and harshness. As to the physical properties, there was decreased in occurrence of flat leaves than that of conventional ones. On the other hand, in case of reducing air flow during the curing process, increase of price per kg reached to about 5 % compared with those of conventional curing method.

• Journal of the Korean Society of Tobacco Science
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• v.4 no.1
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• pp.29-35
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• 1982

Potassium deficiency symptoms were studied with flue-cured tobacco which was applied with different levels of compound fertilizer (10-15-20) ; 75kg, 100hg, and 125kg/10a. Ratio of N/$K_2O$ in leaves was increased from bottom to top stalk position due to the increase of nitrogen content in leaves. Nitrogen content in leaves was increased from stalk to tip as wall as from midrib to laminae, but vice versa in potassium content. Consequently, resulting in potassium deficiency symptoms in tip of leaves. Rate of reabsorption by rainfall during the latter part of growth was highest at top stalk position in case of nitrogen, but lowest in potassium. This observation was more evident with higher application rate of fertilizer. Nitrogen content of about 4 % in leaves of top stalk position applied with 125kg/10a was maintained up to 85days after trans planting. No increase in potassium in upper leaves was observed over the level of 100kg/10a fertilizer application. As the result, N/$K_2O$ ratio in leaves of top stalk position applied with 125kg/10a was kept at more than 1.0 up to 85days after transplanting, but it was less than 0.9 at 65days after tracts planting with less than 100kg/10a fertilizer application.