• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.3
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• pp.351-358
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• 1995

Since summer growth depression of forage crops limits hay production in Korea, it is important to exploit natural resources which are suitable as forage. In the preliminary experiment it was found that Panicum dichotomiflorum Michx. might be utilized as soiling and hay crop. Effects of seed stratificaion, planting date and number of harvestings on fresh and dry yields of Panicum dichotomiflorum Michx. were investigated in 1993 and 1994. Stratification of seed promoted emergence, while seed age decreased emergence. Although fresh yield was closely related with number of plants estabilished per unit area, effect of number of plants per unit area on yield was less at the later stage of growth. Higher cutting height decreased fresh and dry yield at th early stage, but final yields were not influenced by cutting height. Panicum dichotomiflorum Michx. could be harvested 3~4 times beginning 40~50 days after planting and fresh and dry yields were 9~10 tons /10a and 1.4~1.8 tons /10a, respectively. Fresh and dry yields were significantly influenced by growth duration or accumulated temperature between cuttings. Leaf blade ratio was higher at earlier planting and / or harvesting. Contens of crude protein, crude fat and crude ash were 16.32, 24.01 and 11.59%, respectively.

• Journal of agriculture & life science
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• v.50 no.2
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• pp.73-82
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• 2016

Best drought tolerance index was determined through statistics analysis and growth appearance of drought tolerant plants was determined by cultivation in pot and sloping land. For determination of best drought tolerant indicators, RD(Resistant dry days), LD(Leaf area), UTR(Unit transpiration), RWC(Relative water content), RWL(Relative water loss), LA(Leaf area), SN(Stoma unmber) and SA(Stoma area) were carried out by correlation and PCA analysis. RWL and UTR were affected on plant drought tolerance according to comparison among six indices for resistant dry days. The PCs axes separated SA, LA, RD and RWC and SN. UTR was negatively correlated with SA, RWL were also negatively correlated with RWC and SN. RWL and UTR were proved best selection indicator for the selection of drought tolerant species. Ulmus parvifolia, Bidens bipinnata, Patrinia villosa, Kummerowia striata, Arundinella hirta, Artemisia gmelini etc. were selected drought tolerant plants. Shoot growth appearance of drought resistant plants was differed pot and sloping land. Shoot growth and leaf number was no significant differences between the pot and sloping land. However, root growth of drought tolerant plants was all the difference between two cultivation. T/R ratio of drought tolerant plants was also found a big difference. T/R ratio of drought tolerant plants in sloping land was lower than that of pot. These results will be served efficiently plant breeding.

• Korean Journal of Plant Resources
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• v.10 no.2
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• pp.140-144
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• 1997

This experiment was carried out to investigate the proper planting method of Rehmannia glutinasa Liboschitz. A Chungbuk local cultivar was sown on the seed bed of 200cm with the planting density of 30cm(6 rows) $\times$ 8.5cm(30 rhizome per $\textrm{m}^2$). The experimental design was randomized block design with 3 replications. And the result was summarized as follows. The emergence date of level Planting(control : 0$^{\circ}$) was May 23, but the emergence dates of Oblique planting(45$^{\circ}$) and Upright planting(90$^{\circ}$) treatments were later than the control by 2~4 days. And the leaf length and the ratio of leaf length/leaf width were not significantly different. As compared with the 29.2cm and 10.0mm of the rhizome length and the rhizome diameter of level planting treatment, the rhizome length of Oblique planting treatment was decreased by 3.4cm and 0 mm. The length and diameter of rhizome of the Upright planting treatment was decreased by 2.8cm and 1.0mm. And the order of sugar content was level Planting > Oblique planting > Upright planting. The total yields were decreased by 9% in the Oblique planting and by 15% in the Upright planting compared to 1,203kg/10a in the control, and the commercial yields showed the same tendency. The level planting treatment was found to be most proper for sowing of Rehmannia glutinasa Liboschitz in the middle inland region of Korea.

• Journal of Mushroom
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• v.18 no.3
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• pp.268-273
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• 2020

This study aimed to assess the feasibility of composting spent mushroom substrate (SMS) materials of Lentinula edodes (Le), Hericium erinaceus (He), and Pleurotus ostreatus (Po). The different SMSs were composted for 7 to 10 days at high temperatures over 50℃; the composting procedure was completed in 30 days. A maturity test was conducted using the radish seed germination index and CoMMe-100. The composted SMS (CSMS) from Le and He showed gemination indices of 130% and 81%, respectively, that satisfied the criteria of maturity standard (gemination index over 70%) and the CoMMe-100 analysis. The physicochemical changes of CSMSs included an increase in the pH range from 4-5 to 6-7, slight reduction in the EC to 1-1.4 dS/m, and an organic content of 36.9% in LeCSMS. In LeCSMS, the contents of N, P, and K were 1.2%, 2.3%, and 0.77%, respectively, and heavy metals were detected below the standard value in all CSMSs; the Ca and Mg contents in the CSMSs were increased from 30% to 60% when compared to those in the SMSs. The C/N ratio (from 26-33) in LeSMS and HeSMS decreased to 15.3-15.9 in CSMSs. The growth effect of LeCSMS treatment on pepper seedlings was 60% higher than that in the control groups, one of which was treated with commercial organic compost; the former showed a superior growth effect on the leaf width, leaf length, and leaf number compared to other control groups. In conclusion, LeCSMS and HeCSMS could be utilized as compost resources capable of efficient soil amendment and plant growth promotion.

• Journal of Plant Biotechnology
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• v.34 no.1
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• pp.61-68
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• 2007

To develop rice (Oryza sativa L.) cultivars to be planted on salt-affected sites, cell lines with enhanced proline content and resistance to growth inhibition by Azetidine-2-carboxylic acid (AZCA), a proline analogue, were screened out among calli irradiated with gamma ray of 50, 70, 90, and 120 Gy. The calli had been derived from embryo culture of the cultivar Donganbyeo. Selected AZCA resistant lines that had high proline accumulation were used as sources for selection of NaCl resistant lines. To determine an optimum concentration for selection of NaCl resistant lines, Donganbyeo seeds were initially cultured on the media containing various NaCl concentrations (0 to 2.5%) for 40 days, and 1.5% NaCl concentration was determined as the optimum concentration. One hundred sixteen salt-tolerant (ST) lines were selected from bulked 20,000 seeds of the AZCA resistant $M_{3}$ seeds in the medium containing 1.5% NaCl. The putative 33 lines ($M_{4}$ generation) considered with salt-tolerance were further analyzed for salt tolerance, amino acid and ion contents, and expression patterns of the salt tolerance-related genes. Out of the 33 lines, 7 lines were confirmed to have superior salt tolerance. Based on growth comparison of the entries, the selected mutant lines exhibited greater shoot length with average 1.5 times, root length with 1.3 times, root numbers with 1.1 times, and fresh weight with 1.5 times than control. Proline contents were increased maximum 20%, 100% and 20% in the leaf, seed and callus, respectively, of the selected lines. Compared to control, amino acid contents of the mutants were 24 to 29%, 49 to 143%, 32 to 60% higher in the leaf, seed and callus, respectively. The ratio of $Na^{+}/K^{+}$ for most of the ST-lines were lower than that of control, ranging from 1.0 to 3.8 for the leaf and 11.5 to 28.5 for the root, while the control had 3.5 and 32.9 in the leaf and root, respectively. The transcription patterns for the P5CS and NHXI genes observed by RT-PCR analysis indicated that these genes were actively expressed under salt stress. The selected mutants will be useful for the development of rice cultivar resistant to salt stress.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.6
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• pp.534-540
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• 1992

Climatic condition and seeding depth affect the seedling stand and early growth in the direct-seeded rice cultivation on dry soil. This experiment was conducted to elucidate the effects of the day /night temperatures and the seeding depths on the seedling emergence and mesocotyl elongation of rice seed. Three combinations of the day/night temperatures(25/2$0^{\circ}C$ 20/15$^{\circ}C$ and 20/1$0^{\circ}C$) were employed with seeding depths 1, 3, 5 and 7cm at the Phytotron of the Crop Experiment Station in 1991. It appeared that seedling emergence ratio increased and days to seedling emergence decreased in the high temperature (25/2$0^{\circ}C$) and the deep seeding depth (5 and 7cm) condition. The seedling emergence ratio did not. show the, difference up . to the seeding depths of 3cm and below, but the ratio decreased from the seeding depths of 5cm and above. Plant height and leaf number were almost the same up to the depths of 3cm at 30 days after seeding, but those of the seeding depths of 5cm and 7cm were remarkably reduced in all temperature combinations. Mesocotyl and lower internode elongation were seen in the high temperature(25/2$0^{\circ}C$) with the seeding depths of 5cm and 7cm. In the seeding depth of 6cm, of the tested varieties, Tamjinbyeo and Odaebyeo showed the highest emergence ratio. Generally, leaves of all tested varieties appeared approximately in the soil depth of 3cm, so the reasonable seeding depth will be around 3cm in the direct-seeded cultivation on dry soil.

• Korean Journal of Soil Science and Fertilizer
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• v.3 no.1
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• pp.49-54
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• 1970

In an effort of determine the metabolism and bio-synthesis of nitrogen, was studied at variance of souble nitrogens, free amino acids and total alcohol soluble amino acids during the growth of younger soybean plants, and saybean divides into cotyledon and embryonic organ (shoot and root system) in this experiment. 1. In the soluble nitrogen of soybean, ratio of increase and decrease in the amino acids of them was displayed the near phenomena both cotyledon and embryonic organ of soybean. But, in the 17th days after seed germination, that is the developmeatal stage of adult leaf of soybeans, was appear the maximum value. It has been suggested that the stage of first half period of growth as boundary the stage of adult leaf development which indicated clear morphologically, at the younger soybean plants, is the step that nitrogen assimilation. 2. It was investigated the amino acids of seventeen kinds in the seed state, but at the third days after seed germination, was investigated the amino acids of nineteen kinds. Ultimately, it appears the translocation from cotyledon to embryonic organ in the distribution of amino acid, the nineth day which differentiation begining day of embryonic organ, then after, it happen the variation of number of inspected amino acid in the cotyledon and embryonic organ but only the variance changes in the distribution and quantitative aspects. Especially, the most conspicuous fact was indicated the accumulation of Asparagine, that is the phenomena of Asparagine-accumulation was constitute, not with standing no fertilization from outside. It may be concluded from the results of this investigation that the difference of special phenomena of soybean from the embryo of other plans. 3. In the initial stage of differentiation at embryonic organ number of inspected amino acid was very few, and then, it was slightly appeared the increase-phenomena in the number of them. It was that the amino acid inspecting the initial stage was translocated from the cotyledon. It is suggested that the intermediate-metabolism of amino acid was constituted on the basis of above the result. 4. The phenomena of increase and decrease of total alcohol soluble amino acid were essentially identical to the water soluble amino acid of soybean, but it was appeared the severe difference of amounts in both of them.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.1
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• pp.37-44
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• 1992

Germinability, electrophoretic variation of protein of shattered seeds during overwintering and characteristic of main agronomic traits of off-type rice plant occurring in paddy field harvested by combine were investigated. Germinability of shattered seed decreased as time goes in paddy field in both Japonica and Tongil type varieties. Electrophoretic protein bands become more and more light as time goes. Occurrence of off-type rice plant was higher in Japonica varieties than in Tongil type varieties. Off-type rice plant was shorter in plant height, leaf length, tiller number per hill, internode length, panicle length and spike let number per panicle. Germinability of seeds of off-type rice plant as not significantly different compared to the control variety. Yield of off-type plants of Japonica varieties was on average 5.1kg / 10a and that of Tongil type varieties was on average 0.9kg /10a. Mixing ratio to the control variety was about 0.7% in Japonica varieties and that of Tongil type varieties was about 0.1%.

• Molecules and Cells
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• v.40 no.10
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• pp.714-730
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• 2017

Pre-mRNA splicing further increases protein diversity acquired through evolution. The underlying driving forces for this phenomenon are unknown, especially in terms of gene expression. A rice alternatively spliced transcript detection microarray (ASDM) and RNA sequencing (RNA-Seq) were applied to differentiate the transcriptome of 4 representative organs of Oryza sativa L. cv. Ilmi: leaves, roots, 1-cm-stage panicles and young seeds at 21 days after pollination. Comparison of data obtained by microarray and RNA-Seq showed a bell-shaped distribution and a co-lineation for highly expressed genes. Transcripts were classified according to the degree of organ enrichment using a coefficient value (CV, the ratio of the standard deviation to the mean values): highly variable (CVI), variable (CVII), and constitutive (CVIII) groups. A higher index of the portion of loci with alternatively spliced transcripts in a group (IAST) value was observed for the constitutive group. Genes of the highly variable group showed the characteristics of the examined organs, and alternatively spliced transcripts tended to exhibit the same organ specificity or less organ preferences, with avoidance of 'organ distinctness'. In addition, within a locus, a tendency of higher expression was found for transcripts with a longer coding sequence (CDS), and a spliced intron was the most commonly found type of alternative splicing for an extended CDS. Thus, pre-mRNA splicing might have evolved to retain maximum functionality in terms of organ preference and multiplicity.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.552-560
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• 2010

$GA_{4+7}$+BA, a plant growth regulator for induction of feathering in young apple tree and increasing fruit size, was applied by various methods on 'Gala'/M.9 apple trees in high density orchard for 4 years to investigate its effect on fruit and shoot growth. $GA_{4+7}$+BA($80-300mg{\cdot}L^{-1}$) increased fruit length, fruit weight, and L/D ratio regardless of application methods, but it did not affect soluble solid content, acidity, leaf area, and chlorophyll. Seed number was not affected by $GA_{4+7}$+BA application, however, more immature seeds was observed in treated 'Gala' fruit. Shoot growth was increased when spraying $GA_{4+7}$+BA at tree crown but not affected when spraying at fruit directly. More significant fruit growth was observed when $GA_{4+7}$+BA was applied on the fruits between late of May and early of June when fruit cell division ended; however, high concentration of $GA_{4+7}$+BA resulted in lower fruit storability because of lower firmness. Hence, more attention should be paid when applying high concentration of $GA_{4+7}$+BA to small sized fruit cultivars like 'Gala'.