• Title/Summary/Keyword: Leaf starch

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Isolation and Identification of Burkholderia pyrrocinia CH-67 to Control Tomato Leaf Mold and Damping-off on Crisphead Lettuce and Tomato

  • Lee, Kwang-Youll;Kong, Hyun-Gi;Choi, Ki-Hyuck;Lee, Seon-Woo;Moon, Byung-Ju
    • The Plant Pathology Journal
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    • v.27 no.1
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    • pp.59-67
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    • 2011
  • A bacterial strain CH-67 which exhibits antagonism towards several plant pathogenic fungi such as Botrytis cinerea, Fulvia fulva, Rhizoctonia solani, Sclerotinia sclerotiorum, Colletotrichum sp. and Phytophthora sp. was isolated from forest soil by a chitin-baiting method. This strain was identified as Burkholderia cepacia complex (Bcc) and belonging to genomovar IX (Burkholderia pyrrocinia) by colony morphology, biochemical traits and molecular method like 16S rRNA and recA gene analysis. This strain was used to develop a bio-fungicide for the control of tomato leaf mold caused by Fulvia fulva. Various formulations of B. pyrrocinia CH-67 were prepared using fermentation cultures of the bacterium in rice oil medium. The result of pot experiments led to selection of the wettable powder formulation CH67-C containing modified starch as the best formulation for the control of tomato leaf mold. CH67-C, at 100-fold dilution, showed a control value of 85% against tomato leaf mold. Its disease control efficacy was not significantly different from that of the chemical fungicide triflumidazole. B. pyrrocinia CH-67 was also effective in controlling damping-off caused by Rhizoctonia solani PY-1 in crisphead lettuce and tomato plants. CH67-C formulation was recognized as a cell-free formulation since B. pyrrocinia CH-67 was all lethal during formulation process. This study provides an effective biocontrol formulation of biofungicide using B. pyrrocinia CH-67 to control tomato leaf mold and damping-off crisphead lettuce and tomato.

Effect of Canopy Structure on Paddy Yield in Relation to Nutrient Uptake (수도(水稻)의 군락(群落) 및 양분(養分) 흡수(吸收)가 수량(收量)에 미치는 영향)

  • Kwon, Hang Gwang;Kim, Yung Sup;Park, Jung Kew
    • Korean Journal of Soil Science and Fertilizer
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    • v.5 no.1
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    • pp.33-39
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    • 1972
  • For the increase of productivity, canopy structure and nutritional status in various productivity grades (high>700, medium>600 and low>500kg/l0a as head rice) were investigated in a study using Jinheung(Oryza sativa L.) for the increase of producticity with following results: 1. High yield plot showed greater leaf area and slow decrease of it after heading stage. While low yield plot had rapidly decrease of leaf area. 2. High yield plot showed higher light transmission ratio (LTR) durnig early grow stage, low LTR. during late grow stage, and higher leaf area per LTR. 3. Net assimilation ratio was decrease with the increase of leaf area index (LAI) and it was always higher in high yield plot. 4. High yield plant had greater amount of total nitrogen uptake, slow uptake rate, and considerable uptake after heading while low yield plant showed higher uptake of nitrogen in early stage of growth and no uptake after heading. 5. The percent ratio starch at heading to total at harvesting was 40% for low yield plant and 10% for highyield plant inderting that high yield depends much on past-heading starch synthesis. 6. Main factor determining secondary yield was ripened grain ratio.

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Mycorrhizal colonization effects on C metabolism in relation to drought-tolerance of perennial ryegrass (페레니얼 라이그라스에서 Mycorrhiza 접종이 탄수화물대사와 가뭄스트레스 저항성에 미치는 영향)

  • Lee, Bok-Rye;Jung, Woo-Jin;Kim, Dae-Hyun;Kim, Kil-Yong;Shon, Bo-Kyoon;Kim, Tae-Hwan
    • Korean Journal of Soil Science and Fertilizer
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    • v.35 no.4
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    • pp.232-242
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    • 2002
  • To investigate the effects of arbuscular mycorrhizal (AM) fungus (Glomus intraradices) colonization on drought-stress tolerance, leaf water potential, chlorophyll concentration, P content and carbohydrate composition were examined in perennial ryegrass (Lolium perenne L.) plants exposed to drought-stressed or well-watered conditions. Drought stress significantly decreased leaf water potential, P content and leaf growth. These drought-induced damages were moderated by mycorrhizal colonization. Drought stress decreased the concentration of soluble sugars in shoots. AM plants had a higher foliar soluble sugar than non-AM plants under drought stress condition. Drought stress depressed the accumulation of starch and fructan in shoots, but stimulated in roots. Under drought-stressed condition, starch concentration in roots was higher in non-AM plants than in AM plants. Fructan was the largest pool of carbohydrates, showing the highest initial concentration and the highest net increase for 28 days of treatment. Drought stress slightly decreased fructan concentration in shoots, but remarkably increased in roots. Under drought-stressed condition, fructan concentrations in non-AM and AM shoots at day 28 were 18.7% and 13.3% lower than the corresponding values measured at well-watered plants. However, in the roots, fructan accumulation caused by drought was lessen 13.6% by mycorrhizal colonization. The results obtained suggest that mycorrhizal colonization improves drought tolerance of the host plants by maintaining higher leaf water status and P status, and by retaining more foliar soluble sugars.

E3 ligase BRUTUS Is a Negative Regulator for the Cellular Energy Level and the Expression of Energy Metabolism-Related Genes Encoded by Two Organellar Genomes in Leaf Tissues

  • Choi, Bongsoo;Hyeon, Do Young;Lee, Juhun;Long, Terri A.;Hwang, Daehee;Hwang, Inhwan
    • Molecules and Cells
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    • v.45 no.5
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    • pp.294-305
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    • 2022
  • E3 ligase BRUTUS (BTS), a putative iron sensor, is expressed in both root and shoot tissues in seedlings of Arabidopsis thaliana. The role of BTS in root tissues has been well established. However, its role in shoot tissues has been scarcely studied. Comparative transcriptome analysis with shoot and root tissues revealed that BTS is involved in regulating energy metabolism by modulating expression of mitochondrial and chloroplast genes in shoot tissues. Moreover, in shoot tissues of bts-1 plants, levels of ADP and ATP and the ratio of ADP/ATP were greatly increased with a concomitant decrease in levels of soluble sugar and starch. The decreased starch level in bts-1 shoot tissues was restored to the level of shoot tissues of wild-type plants upon vanadate treatment. Through this study, we expand the role of BTS to regulation of energy metabolism in the shoot in addition to its role of iron deficiency response in roots.

Haplotyping and Evolutionary Studies on GBSSII Gene in Korean Rice Collection

  • May Htet Аung;Yong-Jin Park
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.218-218
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    • 2022
  • Granules-bound starch synthase II (GBSSII), one of the isoforms of granule-bound starch synthase (GBSS), is responsible for amylose synthesis by expressing in non-storage tissues such as leaf, stem, root, and pericarp. Up to date, little is known about this gene functions and basic knowledge of heritable characteristics of this gene, GBSSII. We identified functional haplotypes and performed evolutionary analyses on the GBSSII using 374 rice accessions (320 Korean bred and 54 wild) based on the classified groups. A total of 14 haplotypes were found, and almost all haplotypes (13) were functional, carrying 19 non-synonymous SNPs in two exons (exons 1 and 2). The lowest nucleotide diversity was detected in Tropical japonica (0.00145), while the highest pi-value was in Aus (0.01081), illustrating the signal of this gene evolution. The highest Tajima's D value in Aus (1.6380) indicates GBSSII gene domestication signature under balancing selection, while the lowest Tajima's D value in Temperate japonica (-0.8243) highlights that they were under positive selection, which may be purified due to the excess of rare alleles. The highest genetic differentiation was observed between Tropical japonica and aroma (FST = 0.921928). In contrast, the highest interbreed level was detected in Aus-admixture (FST = -0.20157). The genetic relatedness between and or among the wild and cultivated subpopulations was revealed through PCA, population structure, and phylogenetic analyses.

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Growth and Histological Characteristics of Barley (Hordium vulgare L.) Seedling to NaCl Stress (NaCl Stress에 따른 보리 유묘의 생육특성 및 세포학적 반응)

  • Cho, Jin-Woong;Kim, Choong-Soo;Lee, Sok-Young;Park, Ki-Sun
    • Korean Journal of Environmental Agriculture
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    • v.17 no.4
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    • pp.335-340
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    • 1998
  • This study was conducted to determine the morphological responses of barley seedlings to NaCl stress and to investigate histological changes of cells with transmission electron microscope(TEM) after NaCl stress. Plant height and root length of 10-day old barley seedlings with NaCl stress were reduced and inhibition level was found to be more severe in the plant height than in the root length. The leaf length, leaf width and leaf area were shorter as well with NaCl stress than without NaCl stress. However, there was no difference in the number of roots between NaCl treatments. The weight of dry matter decreased at higher NaCl concentrations, especially at 100mM NaCl. The water content of shoots tend to decrease at higher NaCl concentrations, but there was no difference in the water content of roots, The reduced sugar content was greatly increase than starch. Cellulose content was higher in NaCl stressed-plant than control, and tended to decreased at higher NaCl concentrations. Lignin content also decreased NaCl stressed-plant but there was no tendency at NaCl stress concentrations. Electric conductivity of cell sap with seedlings was high with NaCl stressed-plant. Amount of cell sap gradually increased with time in the roots than in the shoots, The grana of chloroplasts was changed by 150mM NaCl concentration. The christe of mitochondria in root meristematic sells ruined in structure and cell wall of leaf and root was also ruined by NaCl stress.

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The Development of the Dietary Fiber Food Frequency Questionnaire and the Dietary Fiber Intake of Middle School Students in the Chungbuk Province (식이섬유용 식품섭취빈도조사지 개발 및 이를 이용한 충북지역 중학생의 식이섬유 섭취 실태)

  • Shin, Na-Shil;Lee, In-Seon;Kim, Hyang-Sook
    • Korean journal of food and cookery science
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    • v.28 no.5
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    • pp.549-557
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    • 2012
  • In this study, the FFQ-50 questionnaire which composed of 50 food items frequently eaten as sources of dietary fiber, was developed and used to estimate the dietary fiber intake of middle school students in the Chungbuk province. According to the survey, the average daily intakes of dietary fiber for the boys and girls were $23.3{\pm}12.3$ g (93.2%) and $20.8{\pm}11.5$ g (104.0%), respectively, showing a significant difference between the gender. The proportions of boys and girls who did not reach to the sufficient dietary fiber intake were 66.2% and 53.9% respectively. The major sources of dietary fiber were grain foods and vegetables, which measured at 77.24%, followed by fruits, seaweeds, tofu and tofu products, potato and starch, and mushrooms. The cooked white rice contributed the highest (17.81%), followed by baechu-kimchi, cooked brown rice, ramyeon (instant noodle), tangerine, sesame leaf jangajji (pickled sesame leaf), topokki (spicy rice cake stir-fry), blanched and seasoned bean sprouts, bread, and radish kimchi. The study showed that the cooked white rice was the major source of dietary fiber in the boys' diet followed by baechu-kimchi, ramyeon, cooked brown rice, tangerine, bread, strawberry, blanched and seasoned bean sprouts, sesame leaf jangajji, radish kimchi. Girls were taking in dietary fiber mostly from boiled white rice followed by baechu-kimchi, boiled brown rice, tangerine, ramyeon, sesame leaf jangajji, topokki, blanched and seasoned bean sprouts, radish kimchi, and boiled barley.

Quality Characteristics of Lotus Leaf Dasik Prpared with Various Sweeteners (당의 종류를 달리한 연잎 다식의 품질 특성)

  • Yang, Mi-Ok
    • Journal of the East Asian Society of Dietary Life
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    • v.19 no.3
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    • pp.437-443
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    • 2009
  • The objective of this study was to evaluate the quality characteristics of lotus leaf Dasik prepared with various sweeteners, including honey (HON), starch syrup (SS), isomaltooligosaccharide(IMOS), galactooligosaccharide (GOS), and fructooligosaccharide (FOS). The moisture contents of samples were highest in GOS. L-values were significantly higher in the HON group. Whereas a- and b-values were not significantly different among the groups. According to the results for mechanical characteristics, hardness was significantly lower in HON while cohesiveness, gumminess, and chewiness were highest in SS; however, adhesiveness and springiness were not significantly different among the groups. The sensory evaluation results showed that the lotus leaf Dasik containing FOS received the highest preference scores.

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Changes of Chemical Components During Seed Development in Black Soybean (Glycine max L.)

  • Shim Sang In;Kang Byeung Hoa
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.4
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    • pp.331-336
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    • 2004
  • Changes in the level of metabolites in leaves and pods were examined with respect to the seed chemical composition in black soybean. There was no further increase in pod length after 42 days after flowering (DAF). Pod weight, however, persistently increase until 73 DAF, thereafter the weight was slightly lowered. The seed storage protein, however, increased drastically as the increasing rate of pod weight was lessened at 61 DAF. The accumulation of seed storage proteins was occurred conspicuously as the increasing rate of pod weight was slowed down. The chlorophyll content both in leaves and pods was drastically decreased after 50 DAF. The beginning of drastic reduction in chlorophyll content was occurred concomitantly with the reduction of soluble protein content in leaves. The sugar content in leaves showed similar tendency with chlorophyll and soluble protein content. The starch level in leaves, however, showed different changing pattern during seed development. The starch content in leaves was increased persistently until 66 DAF, thereafter the content was decreased drastically to about $55\%$ of maximal value at 66 DAF. Total phenolics content in leaves and the anthocyanins content in seeds were stable without noticeable increase until 66 DAF. The contents were increased dramatically after 66 DAF showing the synchronized pattern with the decrease in starch level in leaves. The levels of the selected metabolites in leaf and seed suggested that the accumulation of chemical components of black soybean seed is launched actively at 66 DAF. The profile of storage proteins was nearly completed at 61 DAF because there was no large difference in densitometric intensity among protein subunits after 61 DAF. In soybean, chemical maturation of seed begins around 61 to 66 DAF at which most metabolites in vegetative parts are decreased and remobilized into maturing seeds.

Physico-chemical properties and biological activity of controlled-release granular formulations for the herbicide dicamba (방출조절형 dicamba 입제의 물리화학성 및 생물효과)

  • Oh, Kyeong-Seok;Oh, Byung-Youl;Park, Seung-Soon;Lee, Jae-Koo
    • The Korean Journal of Pesticide Science
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    • v.3 no.1
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    • pp.37-45
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    • 1999
  • Dicamba (3,6-dichloro-o-anisic acid) granular formulations for controlled release (DGFCRs) were prepared with biodegradable polymers, corn starch and pregelatinized starch, to minimize harmful side effects, extend weed control performance, and control the releasing rate of the active ingredient. Physico-chemical properties and biological activity of DGFCRs were studied. Six different granules were formulated by applying two processes, granulation and extrusion. Formulation efficiencies of active ingredient (A.I.) in the granules prepared by granulating and extruding were $90.0{\sim}96.3%$. Incorporation ratios of A.I. in the granules prepared by granulating and extruding showed $89.5{\sim}94.5%$ and $46.7{\sim}82.0%$, respectively. The highest swellability was DG-2 formulation prepared with corn starch. Whereas, the lowest floatability in water was DG-2 formulation, while the highest one was DG-1 formulation prepared with pregelatinized starch, Miragel 463. The degradation rates of dicamba in the granules under the elevated temperature of $50^{\circ}C$ were less than 5% for DG-1 and DG-2 formulations even after 90 days, meanwhile, those of DE-1 formulations prepared with pregelatinized starch, Mirasperse, were more than 5%. The release rates of A.I. from the granules into water under a static condition were about 100% after 2 weeks. Weeding effects of the granules on broad leaf weeds tested in greenhouse were more than 90% after 30 days.

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