• Journal of Bio-Environment Control
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• v.26 no.4
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• pp.361-367
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• 2017

Production cost as well as environmental contamination can be reduced by reuse of drained nutrient solution in hydroponic. This research was conducted to obtain the information in changes in inorganic elements concentration of supplied and drained nutrient solution as well as of plant leaves. To achieve the objective, the samples of supplied and drained solution and cherry tomato leaf tissues were periodically collected and analyzed during the hydroponic cultivation. The electrical conductivity (EC) of supplied and drained nutrient solution in early growth stage of cherry tomato were measured as around $2.0dS{\cdot}m^{-1}$, but those values move up with the passage of time reaching to $2.0dS{\cdot}m^{-1}$ at flowering stage of 9th fruiting node. The pHs of drained solution in early growth stage were 6.4 to 6.7, however those showed a tendency to get lowered to 5.9 to 6.1 as time passed during the crop cultivation. The concentration differences of $NO_3-N$, P, K, Ca, and Mg between supplied and drained solution were not distinctive until flowering stages of 4th fruiting nodes, while those in drained solution moved up after the stage. The tissue N contents of leaves decrease gradually and those of K and Ca increased as crops grew. However, Tissue P and Mg contents were maintained similarly from transplant to end-crop. The above results would be used in correction of drained nutrient solution when element compositions are varied compared to supplied solution in hydroponic cultivation of tomatoes.

• Journal of Life Science
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• v.27 no.6
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• pp.640-645
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• 2017

Clerodendrum trichotomum (CT) leaves and stems have been used in folk medicine for their anti-hypertension, arthritis, rheumatism, and anti-inflammatory properties. This study was performed to evaluate the potential of CT as an anti-oxidant and anti-inflammatory agent. CT leaves were extracted using 70% ethanol (EtOH). Then, using this extract, a hexane, chloroform ($CHCl_3$), ethyl acetate (EtOAc), and n-butanol (BuOH) fraction was prepared. The polyphenol contents were higher in the EtOAc fraction ($78.08{\mu}g/mg$) and BuOH fraction ($77.54{\mu}g/mg$) compared to the other fractions. Also, these two fractions exhibited strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging activities. Xanthine oxidase inhibitory activities were higher in the $CHCl_3$ fraction ($IC_{50}=4.43{\mu}g/ml$) and EtOAc fraction ($IC_{50}=5.69{\mu}g/ml$). Moreover, the EtOAc fraction effectively inhibited nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells ($IC_{50}=18.87{\mu}g/ml$). Thus, we investigated the effects of the EtOAc fraction on the expression of pro-inflammatory cytokines, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) in LPS-stimulated RAW 264.7 cells. The treatment of the EtOAc fraction ($100{\mu}g/ml$) effectively decreased the levels of the tumor necrosis factor ${\alpha}$ ($TNF-{\alpha}$) and interleukin-6 (IL-6), and the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). These results suggest the potential for CT extract and fractions as promising anti-oxidant and anti-inflammatory agents.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.191-191
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• 2017

Hydroxy fatty acid (HFA) is an important industrial resource that known to be extracted from seeds of Castor or Lesquerella. However, mass production of HFA from those crops are difficult because of their behavior or life cycle. In this study, we applied HFA synthesis related gene FAH12, RcPDAT1, RcLPCAT, RcDGAT2, and RcPDCT on bioenergy crop Camelina sativa. Furthermore, we determined NaCl or cold stress tolerance changes of transgenic Camelina. RcFAH12, RcPDAT1, RcLPCAT, RcDGAT2, and RcPDCT genes were cloned into multigene expression vector which is engineered with seed specific promoter of FAE1 or Napin. Combination of HFA genes multi-expression vector constructs were divided into Set3 (RcFAH12, RcPDAT1-2, RcLPCAT), Set4 (RcFAH12, RcDGAT2, RCPDAT1-2, RcLPCAT), and Set5 (RcFAH12, RcDGAT2, RCPDAT1-2, RcLPCAT, RcPDCT). Transgenic HFA synthesis Camelina plants were generated using agrobacterium-mediated vacuum infiltration system. Results of fatty acid composition of T1 transgenic Camelina seeds analyzed by GC-MS showed 9.5, 9.0, and 13.6 % of HFA contents in Set3#6, Set4#8, and Set5#10, respectively. Therefore, seeds of T2 generation were harvest from Set5#10 which is shown highest HFA contents, and, 17.7, 8.1 and 10.5 % of HFA contents were determined in Set5#10-5, Set5#10-8, and Set#10-10, respectively. However, 7.7% of C18:2 and 22.3 % of C18:3 among unsaturated fatty acids were decreased in Set5#10-5 than WT. Meanwhile, we confirmed abiotic stress responses in T2 transgenic Camelina Set5#10-5 and Set5#10-10 under 0, 100, 150, and 200 mM NaCl or 25, 15, and $10^{\circ}C$ temperature for 5 weeks. Both Set5#10-5 and Set5#10-10 showed lower growth in height than WT in control and NaCl condition. Growth of leaf length and width were similar in WT and Set5#10-10 but lower in Set5#10-5 under NaCl stress. Number of opened flowers showed that both transgenic Camelina were lower than WT under normal condition. But, WT and Set5#10-10 showed similar opened flower number in 100 and 200 mM NaCl. In cold stress, 15 and $10^{\circ}C$ treatment for 5 weeks did not showed significant changes in between WT and both transgenic lines even they showed different growth rate in control condition. Taken together, growth and development are delayed by expression of exogenous HFA related genes in transgenic lines but relative abiotic stress sensitivity is similar with WT. In conclusion, reduced C18:2 or C18:3 fatty acid composition of seed by HFA synthesis is resulted from lack of resource supplement for development at seedling stage but it is not affect NaCl and cold stress tolerance.

• Horticultural Science & Technology
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• v.33 no.4
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• pp.511-517
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• 2015

This research was conducted to evaluate the influence of root restriction materials and media on the growth of runner plantlets of 'Seolhyang' strawberry in a nursery field. To achieve this, the influence of three kinds of root media on the growth of runner plantlets was monitored when polyethylene film was used as the root restriction material. In addition, the influence of various root restriction materials (RRS) such as transparent polyethylene film (PE), non-woven fabric (NF), perforated polyethylene film (PP), and root proofing sheet (RPS) on the changes in volumetric water content (VWC) and temperature of root media as well as growth of runner plantlet were investigated when expanded rice hull (ERH) was used as the root medium. In the comparison of root media, growth parameters such as leaf area and crown thickness at 20 d after fixation as well as crown thickness and fresh weights of root and above-ground tissue at 40 d after runner plantlet fixation were higher in the ERH treatment than in sandy loam and loamy sand. When the influence of RRS was compared, the VWC of ERH was 55% just after irrigation, but decreased to 26% at just before irrigation. Ranges of the VWC as influenced by irrigation cycle were 16 to 10% in the PP and less than 10% in the NF and RPS. The soil temperature in the PE treatment was around $1^{\circ}C$ lower than in NF, PP, and RPS. The differences between day and night temperatures were also smaller in the PE treatment rather than those in NF, PP, and RPS. The growths of runner plantlet 50 d after fixation showed that plant heights as well as fresh weights of root and above-ground tissue were higher in the PE treatment than in NF, PP, and RPS. NF and PP did not effectively restrict roots inside the medium and the roots of runner plantlets penetrated through the root restriction materials resulting in the formation of root system below the restriction materials. The above results indicate that ERH is more effective than sandy loam or loamy sand as root medium. PE rather than NF, PP, or RPS as root restriction material resulted in better growth of runner plantlets in propagation of 'Seolhyang' strawberry. The results of this research will be used for production of high quality runner plantlets in strawberry propagation.

• Horticultural Science & Technology
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• v.31 no.2
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• pp.179-185
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• 2013

This study was carried out to find a reasonable irrigation method of a nutrient solution for the phenolic foam slab (foam LC) used in a trial experiment to substitute the rockwool slab in the production of paprika (Capsicum annuum 'Veyron'). 100, 90, and 80 mL of a nutrient solution was supplied per plant each time when the accumulated radiation reached to 100, $90J{\cdot}cm^{-2}$, and they were named as the 100-100, 90-90, and 90-80 treatment, respectively. The drain percentage per plant of the 100-100 treatment was high by 33.8% in rockwool and 36.7% in foam LC (Lettuce Cube) and that of 90-80 treatment was low by 30.4% and 33.7%. The water content and EC of the rockwool slab were maintained in the range of 63.6-68.9% and $4.4-5.1mS{\cdot}cm^{-1}$, while those of the foam LC slab were in the range of 52.9-58.8% and $5.5-6.5mS{\cdot}cm^{-1}$. The plant height and leaf size of the 100-100 and 90-90 treatments increased in a similar manner, while those of the 90-80 treatment decreased and those of the rockwool were greater than those of the foam LC. The fruit size and weight of the 100-100 and 90-90 treatments were similarly bigger and heavier than those of the 90-80 treatment. The number of fruits harvested per plant was the greatest in the 90-80 treatment with 8 and 8.3 fruits in the rockwool and foam LC. The number of marketable fruits in the rockwool and foam LC was the greatest with 18.1 and 18.2, respectively, in the 90-90 treatment, while that in the 90-80 treatment was 17.2 and 16.8, respectively. The number of unmarketable fruits of the 90-80 treatment was the greatest (1.7-1.8 fruits per plant) in both the rockwool and foam LC, and most of them were small sized or blossom end rot fruits. The yield of the 90-90 treatment was the greatest among the irrigation.

• Korean Journal of Soil Science and Fertilizer
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• v.37 no.4
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• pp.228-234
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• 2004

In organic farming, nutrients for the crop production are mostly supplied by compost containing various organic materials. The long-term organic cultivation would result in continuous changes of soil chemical properties and fertility. The aim of this study was to investigate the contribution of long-term organic cultivation to the soil fertility in Korea focusing on the chemical properties of soil. Soil samples were collected from organic farms that had been cultivated for 8-10 years after certification of organic product through the conversion periods of 2-3 years. Thereby each organic farm had acquired optimal cultivating techniques and soil condition. We separated organic farms into three groups by cultivating crops, i.e. leaf vegetables, fruit vegetables and fruit trees. In each group, five representative farms were chosen in order to investigate the relationships between application rate of compost and nutrient contents in soil. The application rate of compost was approximately $10-15Mg\;10a^{-1}$ for the first 2-3 years at the beginning of organic farming and then reduced to a rate of $3-4Mg\;10a^{-1}$ after stabilization of organic matter content in soil with $30-50g\;10a^{-1}$. However, the continuous organic farming for 8-10 years resulted in accumulation of nutrients, especially of P, in soil probably due to the excessive amounts of compost applied. In conclusion, we suggest that the application rate and organic sources of compost should be decided on the basis of P content in soil by soil testing and thereafter the lack of soil N content for crop cultivation should be compensated by crop rotation with such as legumes. This might be an approach to the original meaning of organic farming as an environmental friendly agriculture.

• Journal of Sericultural and Entomological Science
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• v.2
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• pp.49-61
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• 1962

The insect Diplosis mori Yokoyama is causing extensive destruction of mulberry trees in Korea with a resultant loss in silk production. This study was made to determine an effective method of control. Methods and Materials Used Preliminary studies were made to determine more exactly the life cycle of the insect. Based on this information, various control measures were tested, including the use of spray methods with BHC and control of larvae by tilling. Results Obtained 1. Life cycle studies (a) In the Suwon area, this-insect has 5 generations per year. The first starts in the later part of June and the final cycle ends in the later part of September. (b) The adult insects appear about 7: 00-8: 00 P.M. and live for 2-5 days. Females live in longer periods than the male. (c) Larvae lives inside the second and third stipules (A. B.) before mulberry leaf development. They cause extensive damage to the leaves at the point where they are attached to the stem. (d) Weather conditions considerably affect the life cycle. The pupa particularly are affected and not be able to change into the moth stage when there is a long period of no rain. (e) Larvae are large......0.3 to 2.0mm......and are milky-white immediately after hatching but turn to pinkish as the worm matures. The matured worm has a jumping ability up to 15-20cm. The worm burrows into the ground 1.5 to 3.0 cm before changing into the pupal stage. (f) The pupal stage usually lasts 7-8 days, in summer weather conditions and the pupa is surrounded with a coarse cocoon. (g) These insects, as a general rule, overwinter as pupae but sometimes as larvae. 2. Control measures (a) BHC dust applied on the ground seem most effective. It should be done 4-5 days after the worm has burrowed into the ground. For this control, it is recommended that 6kg of a 2% formation Tanbo(l0ares) be used. (b) For the effective spraying against the fly, it is recommended that a formulation of liquid BHC spray terials be used at the rate of 400-600 liters per Tanbo. (c) Tillage methods which provide a cover of soil 5cm or more in depth above infested areas will effect-maively prevent the emergence of the fly from the pupal stage. 3. Conclusions Methods of control against Diplosis mori Yokoyama can be tied more closely to the life cycle of the insect with more effective results. Further studies are needed to complete information on possible controls during or after hibernation. Economic studies on the cost of these control measures are also needed.

• Korean Journal of Soil Science and Fertilizer
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• v.25 no.3
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• pp.231-241
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• 1992

To study the relationships between major micrometeorological elements and their influences on evapotranspiration(ET) in the canopy of two rice cultivars, Daecheongbyo and Samgangbyo, synoptic meteorological factors, micrometeorological elements and ET from the canopy and biomass production were observed at various growth stages in the paddy field of Suwon Weather Forcast Office in 1989. ET from the rice community was highly correlated with the following factors in order of pan evaporation>air temperature>leaf temperature>solar radiation>sunshine duration>difference in vapor pressure depicit(VPD)>water temperature. ET observed showed higher correlation with the evaporation from small pan than that from Class A pan. Varietal difference would be noted in the relationships between ET in Samgangbyo canopy and the evaporations observed from the pans, with which closer a correlation was found in Samgangbyo than in Daecheongbyo. The ratio of canopy ET to the evaporation from Class A pan was maintained over 1.0 through the growth stages with the maximum of 1.9 at the late August. The evaporation observed from Class A pan was amounted to 71.9% of that from small pan. ET was better correlated with solar radiation than with net radiation which reached about 66% of solar radiation. Maximum temperature showed higher correlation with ET than mean air temperature, and also wind speed of 1m above ground revealed positive correlation. The relative humidity, however, had no correlation with the exception of ET in rainy days. A regression model developed to estimate ET as a function of meteorological elements being described with $R^2$ of 0.607 as : $ET=-5.3594+0.7005Pan\;A+0.1926T_{mean}+0.0878_{sol}+0.025RH$.

• Journal of Bio-Environment Control
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• v.18 no.1
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• pp.1-8
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• 2009

The greatest and major cost for cut rose production during winter seasons in Korea is cost of heating the greenhouse. A study was conducted on a cost-efficient heating system to reduce expenses of cut rose growers in times of high energy prices. An infrared heating system utilizing radiant energy has an obvious advantage over other heating methods in that the energy is first used to raise temperatures of plants and other objects and subsequently that of the atmosphere, resulting in faster reaching to desired plant temperatures at a reduced heating cost. In this study the heating effect and heating cost saving of a nano-carbon fiber infrared heating system (NCFIHS) installed in cut rose greenhouses in Gimhae, Gyeongnam Province were analyzed comparatively. In addition growth, quality, and vase life of 'Orange Fresh' roses grown in greenhouses heated by NCFIHS against those grown in greenhouses heated by so called an electrical heating system. In greenhouses with a NCFlHS with a set point air temperature of $20^{\circ}C$, plant temperature was maintained at $1{\sim}2^{\circ}C$ higher than the air temperature, and temperatures of growing bed surface and root zone were maintained at $17{\sim}19^{\circ}C$ throughout cold winter nights. The cost for heating in NCFIHS was about 25 and 51% of that of an electrical heating system and a hot water heating system heated by petroleum, respectively. Growth of roses harvested in greenhouses with a NCFIHS was similar to those grown in greenhouses with an electrical heating system. However, cut roses with more intense petal and leaf colors and a longer vase life (fresh weight and amount of water uptake) were harvested in greenhouses with a NCFIHS as compared to those harvested in greenhouses with an electrical heating system.

• Journal of Bio-Environment Control
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• v.26 no.3
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• pp.175-180
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• 2017

This study was conducted for the domestic new cultivar to expand cultivation area and sustainable production. The new domestic cultivar, 'Jinok' and control cultivar, 'Campbell Early', had been investigated on vine growth and fruit quality by different cultivating conditions as open field, rain shelter and unheated plastic house. The growth period of 'Jinok' was faster than 'Campbell Early'. The harvesting date of 'Jinok' was on 13th of Aug. faster than 'Campbell Early' which was on 17th of Aug., and it was on 25th and 29th of Aug. for 'Jinok' and 'Campbell Early' on field cultivation, respectively. The growing length and width of new shoots were similar among the cropping systems. In the result of fruit characteristic evaluation, the soluble solids content of the 'Jinok' and 'Campbell Early' cultivating on the unheated plastic house were $15.4^{\circ}Brix$ and $15.9^{\circ}Brix$, respectively. In the open field condition, those were $15.9^{\circ}Brix$ and $15.8^{\circ}Brix$, respectively. The titratable acidity and chromaticity were similar among the treatments. In the appearance of past and disease, Phomopsis blight was occurred on mid of June and in the end of cultivation period on about early Aug., the damage by Phomopsis blight was the lowest about 14% in the unheated plastic house cultivation on the 'Jinok' cultivar and it was 39% in the open field condition. However, in the open filed cultivation for 'Campbell Early', Phomopsis blight was highly occurred about 49%. During this period, or the end of cultivation, it is necessary for the intensive control.