• 제목/요약/키워드: Leaf extension

검색결과 478건 처리시간 0.023초

Plant Regeneration through Leaf Explant Culture of Boxthorn (Lycium chinense Mill.)

  • Ham, In-Ki;Park, Sang-Kyu;Lee, Bong-Chun;Lee, Mi-Ae;Kwon, Kyeong-Hak;Lee, Eun-Mo;Jo, Man-Hyun
    • 한국자원식물학회지
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    • 제20권3호
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    • pp.251-254
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    • 2007
  • This study was conducted to investigate the optimal plant growth regulator level for the shoot formation of Lycium chinense Mill. In vitro plant propagation was developed for leaf explants of boxthorn. Leaf explants were cultured on MS medium supplemented with cytokinins (BA and 2-iP) alone. Plants were successfully regenerated through in vitro culture by using leaf explants of boxthorn grown in the field. After 4 weeks of culture, 58% of shoot formation had developed from the leaf explants. The shoot formation rate of 'Jangmyeong' was highest followed by 'Myeongan', ;Cheondae', and 'Bullo'. The use of 0.2mg/L BA was critical for enhanced production of shoot formation and resulted in 58% of the culture producing shoot formations. Regenerated plantlets transplanted to pots were developed and successfully acclimatized to greenhouse.

The identification of optimum condition for direct regeneration in black raspberry

  • Ran, Choi-Heh;Park, Pill-Jae;Lee, Hee-Kwon;Joong, Yun-Song;Lee, In-Sok
    • Journal of Plant Biotechnology
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    • 제35권2호
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    • pp.163-167
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    • 2008
  • Adventitious buds appeared within 2 weeks on the base of the petiole explants and increased for two months. A maximum of regeneration (15.6%) was obtained on the medium containing $1.5\;{\mu}M$ TDZ in combination with $1\;{\mu}M$ IBA. To know which explants are the best for the induction of regeneration, three explants such as leaf, petiole and leaf-petiole were used. Among the explant types, the leaf-petiole explant was significantly more effective than leaf and petiole for promoting adventitious shoots, with leaf-petiole inducing at the highest regeneration frequency (33.7%). The regeneration frequency of adventitious shoots in the leaf-petiole explants was significantly affected by leaf size and the position of explants. The leaf-petiole smaller than 5 mm leaf in width was induced at the highest regeneration frequency (68.9%). The smaller leaf sizes, the greater regeneration frequency. Also when the leaves are nearer to the shoot tip, the regeneration frequency is higher. When the rooted micro-shoots were transferred to the soil after growing for 6 weeks in the media, the survival rate was 90%.

Comparative Performance of Three Tropical Turfgrasses Digitaria longiflora, Axonopus compressus and St. Augustinegrass under Simulated Shade Conditions

  • Chin, Siew-Wai
    • Weed & Turfgrass Science
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    • 제6권1호
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    • pp.55-60
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    • 2017
  • Shade affects turf quality by reducing light for photosynthesis. The shade tolerance of the tropical grasses, Digitaria longiflora and Axonopus compressus were evaluated against Stenotaphrum secundatum (St. Augustinegrass). The grasses were established under shade structures that provide 0%, 50%, 75% or 90% shade level for 30 days. A suite of leaf traits, recorded from similar leaf developmental stage, displayed distinct responses to shade conditions. Leaf length, relative to control, increased in all three species as shade level increased. The mean leaf extension rate was lowest in St. Augustinegrass (80.42%) followed by A. compressus (84.62%) and D. longiflora (90.78%). The higher leaf extension rate in D. longiflora implied its poor shade tolerance. Specific leaf area (SLA) increased in all species with highest mean SLA increase in D. longiflora ($348.55cm^2mg^{-1}$)followed by A. compressus ($286.88cm^2mg^{-1}$) and St. Augustinegrass ($276.28cm^2mg^{-1}$). The highest SLA increase in D. longiflora suggested its lowest performance under shade. The percent green cover, as estimated by digital image analysis, was lowest in D. longiflora (53%) under 90% shade level compared to both species. The relative shade tolerance of the three turfgrasses could be ranked as St. Augustinegrass > A. compressus > D. longiflora.

Clonal Propagation through Leaf Sheath Culture of Phalaenopsis

  • Jo, Man-Hyun;Ham, In-Ki;Lee, Mi-Ae;Han, Gyu-Heung;Woo, In-Shik
    • Plant Resources
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    • 제5권3호
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    • pp.176-180
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    • 2002
  • This study was conducted to develop the clonal propagation technique through in vitro culture using by leaf sheath explants of Phalaenopsis grown in vitro. The highest frequency of protocorm-like body (PLB) formation was obtained when explants of leaf sheath were cultured on VW medium containing 30g/L sucrose, 500 mg/L activated charcoal, 150 mVL coconut water, 1 mg/L NAA, 1 mg/L 2ip and 2.5 g/L gelrite. The PLB formation rate of VW medium was highest followed by modified Hyponex medium, and lowest in MS medium. Plantlets induced from PLBs transferred to plastic pots including spagnum moss were well developed.

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콩 잎 엽모에 의한 불마름병균 부착 저해 (Hairs as Physical Barrier against Adhesion of Xanthomonas axonopodis pv. glycines on Soybean Leaf)

  • 김승한;박석희;우진하;최성용
    • 식물병연구
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    • 제21권1호
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    • pp.40-43
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    • 2015
  • 콩에 발생하는 불마름병은 주로 엽맥을 따라 병징이 형성되며, 이러한 병징의 형성에 대한 원인을 밝히고자 광학현미경 및 주사전자현미경으로 표면을 관찰하였다. 광학현미경 관찰에 의한 조직의 차이는 볼 수 없었으나 주사전자현미경으로 표면 관찰시, 잎 뒷면 미세한 엽모가 잎표면에 존재하였으며 엽맥 주위로는 엽모가 관찰되지 않았다. 세균은 엽맥 주위 엽모가 없는 부분에 주로 부착되어 있었고, 엽모가 있는 부분에서 세균은 관찰되지 않았다. 또한 잎에 불마름병균을 접종 후 표면 미생물을 배양시 엽맥주위에서 주로 세균이 검출되어 엽모의 존재가 불마름병균의 부착을 방해하여 엽맥주변으로 병징이 발현된 것으로 판단된다.

송풍식 줄기절단기에 의한 적정 양파 잎 절단 시기 및 잔여 엽장에 따른 양파 저장 특성 (Appropriate Working Period and Storage Characteristics Based on Residual Leaf Length of Onion (Allium cepa L.) Harvested with a Blower-type Stem Cutter)

  • 민병규;손지영;이미진;문진성;백주희;서재철;신정호;권승귀;홍순중;이상희
    • 생물환경조절학회지
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    • 제33권1호
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    • pp.30-36
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    • 2024
  • 최근 국내에서 개발된 송풍식 양파 줄기절단기를 수확기 양파 엽 절단에 활용할 경우 적정 작업 조건을 구명하기 위해서 이 실험을 수행하였다. 처리구 중 식물체 엽 건조가 가장 많이 진행된(엽 건조 정도 : 66.3%, 엽 수분함량 : 50.5%) 6월 20일 엽 절단 처리구에서 평균 잔여 엽장은 6.7±3.5cm로서 작업 후 적정 잔여 엽장에 해당되는 범위인 4-10cm에 포함되므로 기계 엽 절단 성능이 처리구 중 가장 우수한 것으로 판단된다. 줄기절단기 이용 양파 엽 기계 절단 시 평균 작업 속도는 0.17m·s-1였는데, 이는 인력 엽 절단 처리구의 평균 작업 속도인 0.05m·s-1보다 3.4배 정도 빨랐으며, 이를 통해 해당 기종을 이용하여 10a 면적을 작업할 경우에는 인력 작업(1인 기준)에 비해 2.6시간 정도를 절감할 수 있을 것으로 기대된다. 또한 기계 엽 절단 처리구에서의 손상구 발생률은 1.3%로서 인력 엽 절단 처리구의 0.0%에 비해 높았는데, 이로 인해 기계 엽 절단 처리구가 인력 엽 절단 처리구보다 저장 중 양파 구 부패율이 평균적으로 높았던 것으로 판단된다. 처리별 저장 특성을 살펴보면, 저장 8개월 후 구 부위(기부, 정부)별 부패율은 기계 엽 절단 후 잔여 엽장이 5.0cm 미만인 처리구에서 잔여 엽장이 5.0cm 이상인 처리구보다 높았다. 이는 잔여 엽 길이가 5.0cm 미만인 처리구에서는 5.0cm 이상인 처리구보다 저장 중 구 부패를 유발하는 병원균의 감염이 쉬우므로 장기 저장 시 부패율이 높은 것으로 생각된다. 본 실험 결과와 실험 기종의 성능 목표(작업 후 잔여 엽장 : 5cm) 및 양파 수확 시 적정 잔여 엽장에 관한 기존의 연구결과 등을 종합적으로 고려할 경우, 본 실험에 사용된 줄기절단기 이용 양파 엽 절단 시 양파의 적정 잔여 엽장은 5-10cm 정도일 것으로 판단된다.

Effects of different covering material on stable winter survival management with edible leaf in ramie (Boehmeria nivea L.).

  • Kim, Myeong Seok;An, Ho Sub;Kim, Gil Ja;Kim, Yong Soon;Choi, Jin Gyung;Kim, Dong Kwan;Park, Heung Gyu;Kim, Hyun Woo;Kim, Seong Il
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.226-226
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    • 2017
  • This study was to evaluate methods to high quality food ramie rice cake, thereby increasing farm income. This study investigated the effects of different covering material on stable winter survival management with edible leaf in Ramie(Boehmeria nivea L.). The method of winter survival with covering material were conducted under three condition compose to Non covering, Rice straw cutting covered with 500kg.10a-1, Rice husks covered with 1,000kg.10a-1(covered 4~5 cm thickness in the soil surface). Method of application were standard application(N-P-K-Compost applied at 27-9-27-600kg.10a-1. Compost and fused phosphate applied at 100% of basal fertilizer in March 25. 20% of top dressing were four times application in March 25 - October 5. Planting year were March 15, 2011. Plants were spaced 60 cm apart in rows 25 cm apart with open cultivation. According to non covering < Rice husks covered with 1,000kg.10a-1 < Rice straw cutting covered with 500kg.10a-1 cultivation this order, aerial part as a result were plenty amount of growth. Sprout time and winter survival rates was uncovering control plot compared to 2 - 5 days quickly, 45-57% highly by rice husks and rice straw covering. Green leaf yields is untreated control plot (12,44 kg.10a-1) compared to rice husks covering 7% higher, and rice straw covering increased to 18% of the most.

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잎브로콜리에서 배추좀나방 요방제 수준 설정 (Economic Threshold for the Diamondback Moth (Plutella xylostella L., Lepidoptera: Plutellidae) on Leaf Broccoli (Brassica oleracea var. botrytis italiana))

  • 최선우;임주락;김주희;이공준;문영훈;정성수;이기권
    • 한국응용곤충학회지
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    • 제54권1호
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    • pp.1-6
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    • 2015
  • 잎브로콜리에 심각한 피해를 주는 배추좀나방의 적절한 방제시기 설정을 위한 기초자료로 이용하기 위하여 유충접종밀도와 잎브로콜리 피해량의 관계를 분석하여 경제적피해허용수준과 요방제 수준을 설정한 결과는 다음과 같다. 배추좀나방 3~4령 유충을 주당 0, 0.5, 1.0, 1.5, 2.0마리를 5월 24일에 접종하고, 6월 1일, 6월 9일, 6월 18일에 발생밀도와 엽수량을 조사한 결과, 초기 접종밀도가 증가할수록 배추좀나방의 발생 밀도는 증가하였고 시간이 경과할수록 엽수량은 감소하였다. 이를 토대로 배추좀나방의 접종밀도와 수량감소율을 회귀식을 이용하여 분석한 결과 y=1636-394x ($R^2=0.79^{***}$)로 추정되었다. 잎브로콜리에서 5% 피해율을 경제적 피해한도로 볼 때 배추좀나방의 경제적피해허용수준은 10주당 2~3마리이고, 요방제 수준은 10주당 1-2마리 수준으로 추정된다.

잎절편 배양에 의한 제주 자생나리의 재분화 (Plant Regeneration from Leaf Segments Culture of Several Jeju Native Lilies)

  • 김정선;송승운;김용철;김광호;박영철
    • 한국자원식물학회지
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    • 제18권3호
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    • pp.450-455
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    • 2005
  • 아그로박테리움을 이용한 형질전환연구에 기초자료로 활용코자 기내배양중인 땅나리, 하늘나리, 대만나리의 잎절편으로부터 식물체를 유도하였다. 땅나리 잎절편으로부터 다수의 배양체를 얻기 위해서는 MS배지에 $0.2\;mg{\cdot}L^{-1}\;BA$$0.5\;mg{\cdot}L^{-1}\;NAA$를 첨가하여 암조건에서 배양하는 것이 효과적이었고, 하늘나리 잎 재분화에는 $0.2\;mg{\cdot}L^{-1}\;BA$$2.0\;mg{\cdot}L^{-1}\;NAA$ 혼용처리하는 것이 효과적이었으며, 대만나리의 잎 재분화에는 $1.0\;mg{\cdot}L^{-1}\;BA$$1.0\;mg{\cdot}L^{-1}\;NAA$ 혼용처리가 우수하였다.