Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

The identification of optimum condition for direct regeneration in black raspberry

  • Ran, Choi-Heh (Black Raspberry R&D Team, Gochang Agricultural Extension Center) ;
  • Park, Pill-Jae (Black Raspberry R&D Team, Gochang Agricultural Extension Center) ;
  • Lee, Hee-Kwon (Black Raspberry R&D Team, Gochang Agricultural Extension Center) ;
  • Joong, Yun-Song (College of Agricultural and Life Science, Chonbuk National University) ;
  • Lee, In-Sok (Black Raspberry R&D Team, Gochang Agricultural Extension Center)
  • Published : 2008.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Adventitious buds appeared within 2 weeks on the base of the petiole explants and increased for two months. A maximum of regeneration (15.6%) was obtained on the medium containing $1.5\;{\mu}M$ TDZ in combination with $1\;{\mu}M$ IBA. To know which explants are the best for the induction of regeneration, three explants such as leaf, petiole and leaf-petiole were used. Among the explant types, the leaf-petiole explant was significantly more effective than leaf and petiole for promoting adventitious shoots, with leaf-petiole inducing at the highest regeneration frequency (33.7%). The regeneration frequency of adventitious shoots in the leaf-petiole explants was significantly affected by leaf size and the position of explants. The leaf-petiole smaller than 5 mm leaf in width was induced at the highest regeneration frequency (68.9%). The smaller leaf sizes, the greater regeneration frequency. Also when the leaves are nearer to the shoot tip, the regeneration frequency is higher. When the rooted micro-shoots were transferred to the soil after growing for 6 weeks in the media, the survival rate was 90%.

Keywords

References

  1. Antonelli M, Druart P (1990) The use of a brief 2,4-D treatment to induce leaf regeneration on Prunus canescens Bois. Acta Hort 280: 45-50
  2. Cousineau JC, Donnelly DJ (1991) Adventitious shoot regeneration from leaf explants of tissue cultured and greenhouse-grown raspberry. Plant Cell Tiss Org Cult 27: 249-255 https://doi.org/10.1007/BF00157588
  3. Dubois LAM, de Vries DP (1995) Preliminary report on direct regeneration of adventitious buds on leaf explants of in vivo grown glass house rose cultivars. Gartenbauwissenschaft 60: 249-253
  4. Escalettes V, Dosba F (1993) In vitro adventitious shoot regeneration from leaves of Prunus spp. Plant Sci Limerick 90: 201-209 https://doi.org/10.1016/0168-9452(93)90241-Q
  5. Fasolo F, Zimmerman RH, Fordham I (1989) Adventitious shoot formation on excised leaves of in vitro grown shoots of apple cultivars. Plant Cell Tiss Org Cult 16: 75-87 https://doi.org/10.1007/BF00036516
  6. Feucht W, Schmid PPS, Schimmelpgeng H, Theiler-Hedtrich C, Schmitt A (1985) In vitro vermehrte himbeeren im groBversuch: Abweicherquote vom sortentypus von 0.68 prozent und enzyme-muster der atypischen fruchte. Erwerbsobstbau 27: 167-169
  7. Fiola JA, Swartz HJ (1986) Somatic embryogenesis, organogenesis and proliferation in vitro from Rubus embryos. Acta Hort 183: 91-98
  8. Fiola JA, Hassan MA, Swartz HJ, Bors RH, McNicols R (1990) Effect of thidiazuron, light fluence rates and kanamycin on in vitro shoot organogenesis from excised Rubus cotyledons and leaves. Plant Cell Tiss Org Cult 20: 223-228
  9. Gingas VM, Stokes BD (1993) Rubus plat regeneration via asexual embryogenesis. HortScience 28: 58
  10. Margara J (1982) Bases de la multiplication vegetative. Les meristemes et l'organogenese, INRA 149, Rue de grenelle, 75341 Paris Codex 07, France
  11. McNicol RJ, Graham J (1990) In vitro regeneration of Rubus from leaf and stem segments. Plant Cell Tiss Org Cult 21: 45-50 https://doi.org/10.1007/BF00034490
  12. Owensy de Novoa C, Conner AJ (1992) Comparison of in vitro shoot regeneration protocols from Rubus leaf explants. N. Z. J. Crop Hort Sci 20: 471-476 https://doi.org/10.1080/01140671.1992.10418068
  13. Pratap KP, Madhu S, Anil S, Ahuja PS (2004) Direct shoot regeneration from leaf explants of Rosa Damascena Mill. In Vitro Cell Dev Biol-Plant 40: 192-195 https://doi.org/10.1079/IVP2003503
  14. SAS Institute Inc (1987) SAS/STATTM Guide for Personal Computers, Version 6 Edition. Cary NC SAS Institute Inc: 1028
  15. Swatz HJ, Borsn R, Mohamed F, Naess SK (1990) The effect of in vitro pretreatments on subsequent shoot organogenesis from excised Rubus and Malus leaves. Plant cell Tiss Org Cult 21: 179-184 https://doi.org/10.1007/BF00033439
  16. Welander M, Maheswaran G (1992) Shoot regeneration from leaf explants of dwarfing apple rootstocks. J. Plant Physiol 140: 223-228 https://doi.org/10.1016/S0176-1617(11)80939-9