• Plant Resources
• /
• 제2권2호
• /
• pp.133-138
• /
• 1999

Many higher fungi were collected at Pyonsan penisula Mt.Odae, Korean Highway Cororation Arboretum, Mt.Moak, Yaksan island from 1996 to 1998. They were identified and according to the results, Hygrocybe coccineocrenata, Collybia neofusipes, Marasmius wettsteinii, Amanita esculenta, Lepiota fuscipes, Leucocoprinus subglobisporus, Cystoderma japonicum and Coprinus narcoticus are newly to Korea. They were designed Korean common name by author.

• BMB Reports
• /
• 제31권4호
• /
• pp.355-363
• /
• 1998

MHC unrestricted, antigen nonspecific killing by $CD4^+$ T-cells against virally-infected target cells was induced following cross-linking of CD4 molecules. The cytotoxicity of antibody-activated $CD4^+$ T-cells was abolished by genistein (4',5,7-trihydroxyisoflavone), a protein tyrosine kinase (PTK) inhibitor, but not by H-7, a protein kinase C (PKC) inhibitor. Genisteintreated human or bovine peripheral blood $CD4^+$ T-cells lacked PTK activity and failed to kill virally-infected target cells even after cross-linking of CD4 molecules. The cross-linking of CD4 molecules did not induce effector cell proliferation or the transcription of TNF ${\beta}$. TNF ${\beta}$ synthesis was up-regulated by incubating antibody activated effector cells with bovine herpesvirus type 1 (BHV-1) infected D17 target cells. Anti-TNF ${\beta}$ antibody partially abrogated direct effector cell-mediated antiviral cytotoxicity. On the other hand, this antibody effectively neutralized antiviral activity of effector and target cell culture supernatants against BHV-1 infected D17 cells. The inhibition level of the antiviral activity by the antibody was dependent on effector and target cell ratio. These findings have importance to define the mechanisms of how CD4 cytotoxic cells control viral infection.

• BMB Reports
• /
• 제34권6호
• /
• pp.537-543
• /
• 2001

In the present study, an in vitro ELISA system to assess the interaction between Src homology (SH)2 domains and phosphotyrosine that contain peptides was established using purified GST-conjugated SH2 proteins and synthetic biotinylated phosphotyrosine that contain oligopeptides. The SH2 domains bound the relevant phosphopeptides that were immobilized in the streptavidin-coated microtiter plate in a highly specific and dose-dependent manner. The epidermal growth factor receptor (EGFR)-, T antigen (T Ag)-, and platelet-derived growth factor receptor (PDGFR)-derived phosphopeptides interacted with the growth factor receptor binding protein (Grb)2/SH2, Lck/SH2, and phosphatidyl inositol 3-kinase (PI3K) p85/SH2, respectively. No cross-reactions were observed. Competitive inhibition experiments showed that a short phosphopeptide of only four amino acids was long enough to determine the binding specificity. Optimal concentrations of the GST-SH2 fusion protein and phosphopeptide in this new ELISA system for screening the binding blockers were chosen at 2nM and 500nM, respectively. When two candidate compounds were tested in our ELISA system, they specifically inhibited the Lck/SH2 and/or p85/SH2 binding to the relevant phosphopeptides. Our results indicate that this ELISA system could be used as an easy screening method for the discovery of specific binding blockers of protein-protein interactions via SH2 domains.

• IMMUNE NETWORK
• /
• 제9권2호
• /
• pp.53-57
• /
• 2009

Engagement of the immunoreceptors initiates signaling cascades resulting in lymphocyte activation and differentiation to effector cells, which are essential for the elimination of pathogens from the body. For the transduction of these immunoreceptor-mediated signals, several linker proteins termed transmembrane adaptor proteins (TRAPs) were shown to be required. TRAPs serve as platforms for the assembly and membrane targeting of the specific signaling proteins. Among seven TRAPs identified so far, LAT and LIME were shown to act as a positive regulator in TCR-mediated signaling pathways. In this review, we will discuss the functions of LAT and LIME in modulating T cell development, activation and differentiation.

• 제어로봇시스템학회논문지
• /
• 제8권1호
• /
• pp.60-66
• /
• 2002

In this paper, a new target pointing guidance algorithm is proposed by combining the optimal target pointing solution and a simple time-to-Go estimator. Also investigated are some properties of the guidance algorithm which include a relation to conventional PNG, convergence region and convergence trajectories of error states according to the time-to-go estimator gain. Some guidelines for designing the pointing guidance law are commented based on the convergence properties. A design example in the case of large initial heading errors is presented and its performance is investigated by simulation.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 2004년도 하계학술대회 논문집 A
• /
• pp.333-335
• /
• 2004

전기부하설비의 전압, 전류, 전력, 고조파등의 분석을 위해서는 수학적 모델링 보다는 실제 계측을 통한 전력분석이 필요하고 이론 통해 전기설비의 효율적인 관리, 에너지 절약 및 사고 예방에 필요한 대책을 수립할 수 있다. 특히 전력 및 고조파 유입 유출 등을 분석하기 위해서는 여러 지점의 전력분석을 위한 다채널 데이터 획득시스템은 매우 필수적이다. 따라서 본 논문에서는 전압 및 전류를 다채널로 동시에 측정할 수 있는 데이터 획득 시스템을 DSP 기반으로 개발하였다.

• 한국산학기술학회논문지
• /
• 제20권9호
• /
• pp.211-226
• /
• 2019

백혈구 공통 항원인 돼지 CD45는 PTPRC 유전자에 암호화 되어 있으며, CD45엑손의 선택적 스플라이싱에 따라 다른 T-세포에서 발현되는 티로신 인산분해효소이다. CD45는 기질인 TCR의 $CD3{\zeta}$ 사슬, Lck, Fyn, Zap-70 kinase의 인산화된 티로신에서 인산을 분해하여 T-세포 항원 수용체(TCR) 매개 신호전달을 조절한다. CD45의 조절이상은 많은 면역 질환과 관련이 있어서, CD45는 면역약물 개발에 표적이 되어왔다. TCR 신호전달의 조절효과를 가진 주요 구조적 특징을 특성화하기 위해, 사람의 알려진 CD45 구조를 템플릿으로 적용하여 돼지 CD45RO(가장 작은 CD45 isoform)의 단백질 구조와 예측된 돼지 CD45RO 모델구조에 $CD3{\zeta}$ 사슬의 ITAM(REEpYDV)를 도킹하여 CD45RO/ITAM 펩타이드 결합구조를 예측하였다. 돼지 CD45RO의 구조적 특징은 세포외영역의 구조견고성과 세포질 내 티로신 인산분해효소 도메인의 KNRY와 PTP signature 기능모티프(두 기능 모티프는 ITAM 펩타이드 결합부위의 좁은 입구로 역할)에 있었다. 주요 구조특성은 돼지 CD45RO-ITAM 펩타이드 결합구조 안정성과 결합친화력을 조절하면서 기질 선택성에 영향을 준다. 돼지 CD45RO의 구조적 특성은 T-세포에 특이적인 면역 조절제를 탐색하는 데에 적용될 것이다.

• 조명전기설비학회논문지
• /
• 제19권1호
• /
• pp.155-161
• /
• 2005

사무용 빌딩의 비선형 전자부하에 기인한 전압 및 전류파형의 왜곡은 중성선의 과열, 변압기 손실, 누전차단기 오동작 등을 야기 시킨다. 본 논문에서는 사무용 빌딩에서의 전압 및 전류 고조파의 크기, 전압 및 전류 파고율(Crest Factor : CF), 전압 및 전류 불평형 등을 비교함으로써 파형왜곡 특성을 분석하였다. 분석 결과, 고조파에 의한 상 및 중성선에서의 심각한 파형 왜곡 문제와 삼상에서의 단상 부하의 부적절한 분배로 인한 전류 불평형이 심한 빌딩이 조사되었다. 본 연구 결과는 사무용 빌딩에서 합리적이고 경제적인 부하운용을 위해 사용될 수 있을 것이다.

• Journal of Ginseng Research
• /
• 제19권2호
• /
• pp.117-121
• /
• 1995

We studied the effects of ginsenoside-$Rg_1$ (G-$Rg_1$) extracted from Panax ginseng C.A. Meyer on $p56^{kk}$ kinase and cell proliferation in Jurkat T cells. $p56^{kk}$ was maximally activated within 5 min after the treatment of 16.7 $\mu\textrm{g}$/ml of G-$Rg_1$ increasing the activity by 1.2-2 times relative to untreated control, thereafter its activity was gradually decreased to the level of untreated control. The action of EGTA on the kinase was altered by the addition of G-$Rg_1$, accompanying the band shift of $p56^{kk}$ to $p60^{kk}$. In addition, G-$Rg_1$promoted cell proliferation in a concentration-dependent manner. These results suggest that G-$Rg_1$ may be involved in T cell receptor-CD3 (TCR) signaling via the activation of $p56^{kk}$ and the chance of cellular calcium concentration.

• Animal cells and systems
• /
• 제3권3호
• /
• pp.331-336
• /
• 1999

p56$^{Ick}$, a cytoplasmic protein tyrosine kinase of the src family, is non-covalently associated with the cell surface coreceptors CD4 and CD8, which are expressed on thymocytes and mature T cells. The coreceptor protein plays an important role during the differentiation of thymocytes and the activation of T cells. DNA constructs were designed to study the roles of CD4 and CD8 during the differentiation of thymocytes. One is a chimeric cDNA which consists of coding regions for the extracellular domain of CD8a and the transmembrane and cytoplasmic domain of CD4. The other is the same chimeric cDNA but with a point mutation converting Cys to Ala in the Ick-binding site to disrupt the association. We confirmed that the CD8a/CD4 chimeric molecule bound to Ick more efficiently than the wild type CD8a protein. However, the chimeric protein with the Cys$leftrightarro$Ala mutation did not associate with Ick. The results suggest a possibility that the CD8a/CD4 chimeric protein may behave like a CD4 protein in associating with Ick and that it may deliver a signal inside the cell in a similar manner, Analysing effects of the mutant CD8a/CD4 chimeric protein expression in developing thymocytes will elucidate the role of Ick during the determination of CD4/CD8 cell lineages.