• Title/Summary/Keyword: Lauric acid

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Lysis Action of Lauric Acid on Bacillus subtilis var. niger (Bacillus subtilis var. niger에 대한 Lauric Acid의 용균작용)

  • 조석금;조효현
    • The Korean Journal of Food And Nutrition
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    • v.3 no.1
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    • pp.23-28
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    • 1990
  • Effect of lauric acid and whir derivatives on the growth of Bacillus subtilis var niger was studied. Lauric acid showed the strongest inhibition among the fatty acids tested, Lysis rate of lauric acid proved to be greatly sensitibility against logarithmic phase cells but was not so influenced by cell concentration. On the other hand, lauric acid was inhibited lysis activity when the pH shift from 7.0 to 5.5

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A study on convective heat transfer with microcapsulated lauric acid slurry in circular pipe (미립피복 로릭산 슬러리의 관내 대류 열전달에 관한 연구)

  • Jeong, Dong-Ju;Choi, Eun-Soo
    • Proceedings of the KSME Conference
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    • 2003.04a
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    • pp.1554-1559
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    • 2003
  • The objective of the present study is to reveal thermal characteristic of microcapsulated lauric acid slurry, which have high latent heat during phase change from solid to liquid, in circular pipe. Test were performed with microcapsulated lauric acid slurry in a heating test section with a constant heat flux boundary condition. Local Nusselt number and the effective thermal capacity were measured. As the size of microcapsulated lauric acid were increased, Local Nusselt number of microcapsulated lauric acid slurry were increased. The effective thermal capacity of microcapsulated lauric acid slurry was 0.5 times than it of water

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A Study on Convective Heat Transfer of Microcapsulated Lauric Acid Slurry in Laminar Flows Through a Circular Pipe (미립피복 로릭산 슬러리의 층류 관내 대류 열전달에 관한 연구)

  • Choi Eunsoo;Jung Dongju
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.16 no.11
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    • pp.1006-1012
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    • 2004
  • The objective of the present study is to reveal thermal characteristic of micro-capsulated lauric acid slurry, which has high latent heat during phase change from solid to liquid, in circular pipe. Tests were performed with the microcapsulated lauric acid slurry in the heating test section with a constant heat flux boundary condition. Local Nusselt number and the effective thermal capacity were measured. As the sizes of microcapsulated lauric acids were increased, local Nusselt numbers of microcapsulated lauric acid slurries were increased. The effective thermal capacity of microcapsulated lauric acid slurry was 1.43 times larger than that of water.

Influence of Oleic and Lauric Acid on the Stability of Magnetorheological Fluids

  • Huang, Yuehua;Jiang, Yuhuan;Yang, Xiongbo;Xu, Ruizhen
    • Journal of Magnetics
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    • v.20 no.3
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    • pp.317-321
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    • 2015
  • In this paper, magnetorheological fluids (MRFs) based on micro-sized iron particles dispersed in silicone oil are presented. The iron particles are modified by adding different ratios of oleic acid and lauric acid as surfactants to the suspensions. Lauric acid was found to reduce the stability of the MRFs, and more lauric acid results in a higher rate of sedimentation. Further study showed that the formation and structure of lauric acid may result in the sedimentation of micrometer-sized particles. Meanwhile, the electro-resistance of MRF in this paper shows a decrease from beyond $6000M{\Omega}$ to $190{\Omega}$ with an increase in the external field from 0 mT to 400 mT.

Influences of Acetic Acid, Lauric Acid and Monolurine Treatments on Survival of Vibrio cholerae in Refrigerated Flatfish (냉장광어에서 Vibrio cholerae에 대한 아세트산, Lauric acid 및 Monolaurine의 영향)

  • 이재일;정병철;김창렬
    • The Korean Journal of Food And Nutrition
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    • v.11 no.6
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    • pp.662-666
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    • 1998
  • The effects of 0.5∼1.0% acetic acid, 0.5% laurice acid, or 0.5% monolaurine against Vibrio cholerae non 01 in flatfish strips stored at 15$^{\circ}C$ were assessed. Control strips were dipped in diatilled water only for 3 min. All treatments significantly (P<0.05) reduced the levels of V. cholerae at initial day. The counts of V. cholerae in flatifish treated with either lauric acid or monolurine were a significantly different (P<0.05) from those of acetic acid treatment after 2 days of storage. The counts of V. cholerae in treatments of 0.5% laurice acid after dipping in 1.0% acetic acid for 3 min were lower than those of treatments with 0.5% luarice acid for 3 min after dipping in 0.5% acetic acid for 3 min. Treatments with 0.5% monolurine for 3 min were not effective in lowering (P<0.05) the counts of V. cholerae after 3 days compared to the control.

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Functional Characterization of Drosophila melanogaster CYP6A8 Fatty Acid Hydroxylase

  • Sang-A Lee;Vitchan Kim;Byoungyun Choi;Hyein Lee;Young-Jin Chun;Kyoung Sang Cho;Donghak Kim
    • Biomolecules & Therapeutics
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    • v.31 no.1
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    • pp.82-88
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    • 2023
  • Genomic analysis indicated that the genome of Drosophila melanogaster contains more than 80 cytochrome P450 genes. To date, the enzymatic activity of these P450s has not been extensively studied. Here, the biochemical properties of CYP6A8 were characterized. CYP6A8 was cloned into the pCW vector, and its recombinant enzyme was expressed in Escherichia coli and purified using Ni2+-nitrilotriacetate affinity chromatography. Its expression level was approximately 130 nmol per liter of culture. Purified CYP6A8 exhibited a low-spin state in the absolute spectra of the ferric forms. Binding titration analysis indicated that lauric acid and capric acid produced type I spectral changes, with Kd values 28 ± 4 and 144 ± 20 µM, respectively. Ultra-performance liquid chromatography-mass spectrometry analysis showed that the oxidation reaction of lauric acid produced (ω-1)-hydroxylated lauric acid as a major product and ω-hydroxy-lauric acid as a minor product. Steady-state kinetic analysis of lauric acid hydroxylation yielded a kcat value of 0.038 ± 0.002 min-1 and a Km value of 10 ± 2 µM. In addition, capric acid hydroxylation of CYP6A8 yielded kinetic parameters with a kcat value of 0.135 ± 0.007 min-1 and a Km value of 21 ± 4 µM. Because of the importance of various lipids as carbon sources, the metabolic analysis of fatty acids using CYP6A8 in this study can provide an understanding of the biochemical roles of P450 enzymes in many insects, including Drosophila melanogaster.

Phase-change Temperature of Micro-encapsulated Phase-change Material (미립 피복 상변화 물질의 상변화 온도에 대한 연구)

  • 최은수
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.14 no.2
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    • pp.168-174
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    • 2002
  • In order to obtain a new heat transfer fluid having a high thermal capacity, micro-capsules of a phase-change material can be a successful candidate to be added into water. In this study, 25, 50, 100, and $200\mu$m diameter micro-encapsulated Lauric acids were tested by a differential scanning calorimeter. The Lauric acid itself had a single freezing curve, but the micro-encapsulated Lauric acid had double freezing curves. The second freezing dominated for $25\mu$m diameter Lauric acids. But the first freeing energy became big as the size of the capsule increased.

Dispersion of Li[Ni0.2Li0.2Mn0.6]O2 Powder by Surfactant for High-power Li-ion Cell

  • Yun, Su-Hyun;Park, Yong-Joon
    • Bulletin of the Korean Chemical Society
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    • v.30 no.7
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    • pp.1598-1602
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    • 2009
  • The particle size of Li[$Ni_{0.2}Li_{0.2}Mn_{0.6}]O_2$ cathode powder was controlled effectively by dispersion using lauric acid as a surfactant. The samples treated by lauric acid showed smaller particles of approximately half the original size compared to the particles of a pristine sample. A structural change due to the dispersion of Li[$Ni_{0.2}Li_{0.2}Mn_{0.6}]O_2$ powder was not detected. The rate performance of the Li[$Ni_{0.2}Li_{0.2}Mn_{0.6}]O_2$ cathode was improved by dispersion using lauric acid, which was likely due to the decrease of the particle size. In particular, a sample dispersed pristine powder using lauric acid (L2) presented a greatly enhanced discharge capacity and capacity retention at a high C rate. The discharge capacity of a pristine sample was only 133 m$Ahg^{-1}$ (3C rate) and 96 m$Ahg^{-1}$ (12C rate) at the tenth cycle. In contrast, the L2 electrode delivered higher discharge capacities of 160 m$Ahg^{-1}$ (3C rate) and 129 m$Ahg^{-1}$ (12C rate) at the tenth cycle. The capacity retention at a rate of 12C/2C was also enhanced from ~ 45% (pristine sample) to 57% (L2) by treatment with lauric acid.

Regioselective Oxidation of Lauric Acid by CYP119, an Orphan Cytochrome P450 from Sulfolobus acidocaldarius

  • Lim, Young-Ran;Eun, Chang-Yong;Park, Hyoung-Goo;Han, Song-Hee;Han, Jung-Soo;Cho, Kyoung-Sang;Chun, Young-Jin;Kim, Dong-Hak
    • Journal of Microbiology and Biotechnology
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    • v.20 no.3
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    • pp.574-578
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    • 2010
  • Archaebacteria Sulfolobus acidocaldarius contains the highly thermophilic cytochrome P450 enzyme (CYP119). CYP119 possesses stable enzymatic activity at up to $85^{\circ}C$. However, this enzyme is still considered as an orphan P450 without known physiological function with endogenous or xenobiotic substrates. We characterized the regioselectivity of lauric acid by CYP119 using the auxiliary redox partner proteins putidaredoxin (Pd) and putidaredoxin reductase (PdR). Purified CYP119 protein showed a tight binding affinity to lauric acid ($K_d=1.1{\pm}0.1{\mu}M$) and dominantly hydroxylated (${\omega}-1$) position of lauric acid. We determined the steady-state kinetic parameters; $k_{cat}$ was 10.8 $min^{-1}$ and $K_m$, was 12 ${\mu}M$. The increased ratio to $\omega$-hydroxylated production of lauric acid catalyzed by CYP119 was observed with increase in the reaction temperature. These studies suggested that the regioselectivity of CYP119 provide the critical clue for the physiological enzyme function in this thermophilic archaebacteria. In addition, regioselectivity control of CYP119 without altering its thermostability can lead to the development of novel CYP119-based catalysts through protein engineering.

The Composition of Useful Medium Chain Fatty Acids in Eight Plant Species (특이지방산의 탐색과 붓꽃류에서 중쇄지방산(myristic acid)의 확인)

  • Kim, Jong-Bum;Kim, Kyung-Hwan;Hwang, Sun-Kap;Kim, Yong-Hwan;Cho, Kang-Jin;Hwang, Young-Soo;Park, Ro-Dong;Kim, Jung-Bong
    • Applied Biological Chemistry
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    • v.44 no.1
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    • pp.20-23
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    • 2001
  • Several unusual fatty acids including myristic acid (14 : 0) and lauric acid (12 : 0) were investigated in the Iris family and other related plants. Especially the roots of Iris tecorum contained 75.9% myristic acid in total fatty acid contents and that of Iris germanica contained 57% myristic and 15.5% lauric acid (12 : 0) whereas 10.7% lauric acid and 9.5% capric acid (10 : 0) were detected in the roots of iris ensata as compared to the total fatty acid contents. The total fatty acid contents in the seeds of Foeniculi fructus and Torilis japonica was relatively higher 193.3 mg/g dry wt and 128.2 mg/g dry wt, respectively. 64.5% linoleic acid (18 : 2) and 48.9% ${\alpha}-linolenic$ acid (18 : 3) were observed in the seeds and leaves of iris tectorum whereas its lateral roots contained 9.5% caprylic acid (8 : 0) and 8.6% capric acid. The percentage of myristic acid of the total fatty acid in the immature seeds of iris tectorum and Iris germanica was 10.8% and 15.6%, respectively.

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