• Journal of Korea Water Resources Association
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• v.35 no.2
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• pp.195-201
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• 2002

In this study, an equation is proposed to estimate the limit velocity for lateral stable bed in a curved channel stream. The stable bed on lateral direction is satisfied when there is no more deformation occurs on the transverse bed slope and non-scouring condition in a bend. A theoretical equation for limit velocity is derived using a transverse bed slope model. So, the limit velocity has its theoretical background in the equilibrium of two forces, lateral shear force at the bed due to longitudinal flow and the corresponding lateral bed shear force. To verify the equation, data from four natural river channels were used. There is good agreement between the calculated values using this equation and the measured values. The corrections in equation was found to be correlated with the averaged particle Froude number.

• Journal of Korea Water Resources Association
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• v.49 no.12
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• pp.1015-1025
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• 2016

In general, manholes installed as urban drainage facilities are a variety forms such as straight path manholes, 90 degree bend manhole, three-way combining manhole, and four-way combining manhole. In particular, the surcharged flow at a four-way manholes installed in the downstream of urban sewer system is the main cause of the urban inundation caused by the energy loss. Therefore, it is necessary to analyze the flow characteristics and estimate the head loss coefficients at surcharged four-way combining manholes. The hydraulic experimental apparatus which can change the manhole shapes (square, circle) and flow ratios were installed to estimate the head loss coefficients. In the experiments, two inflows ($Q_m$, $Q_{lat}$) were varied from 0 to $4.8{\ell}/sec$ and 24 combinations were tested in total. The flow ratios $Q_{lat}/Q_{out}$ were varied from 0 to 1 for a total flow $Q_{out}$ ($Q_{out}=Q_m+2Q_{lat}$) of 2, 3, 4, and $4.8{\ell}/sec$, respectively. The variation of head losses were strongly influenced by the lateral inflow because the head loss coefficient increases as the flow ratios $Q_{lat}/Q_{out}$ increases. It was estimated head loss coefficients of the circular manhole is slightly lower than those of the square manhole. However, there was no significant difference of head loss as discharges change. The range of head loss coefficients at four-way combining manhole according to the change of the lateral inflow ratio was estimated to be 0.4 to 0.8. Also, the relation equations between the head loss coefficients (K) and the lateral inflow ratios ($Q_{lat}/Q_{out}$) were suggested in this paper.

• Journal of Korea Water Resources Association
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• v.32 no.1
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• pp.83-94
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• 1999

In continental margins, turbid underflows which are not confined to a given channel, are free to spread laterally as well as longitudinally. Lateral spreading can reduce substantially the run out distance of flows along continental shelves and slopes. Laboratory experiments with a large tank, employing saline density currents as surrogates for fine-grained turbidity flow, coupled with dimensional analysis, have been used to develop a simple expression for lateral spreading rates of two-dimensional flows on sloping beds. characteristic length and time are determined by the flow discharge and buoyancy flux at the inlet. By knowing the initial width of the flow, the spreading law can be used to estimate the maximum width of the current at different times as well as the longitudinal spreading rate. Predictions for flows compare favorably against observations.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.32 no.4
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• pp.73-79
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• 2004

An experimental and computational study has been performed for investigation of the jet interaction in supersonic flow with a ramp located behind a sonic, lateral jet. The experimental techniques include schlieren, pressure taps, and Pressure Sensitive Paint. The numerical solver used in this study is AeroSoft's structured flow solver GASP Version 4.0. A Mach 4 crossflow with a pressure ratio of 532, and the 3D ramp was designed by parametric study using GASP. The results showed that the ramp located downstream of the jet decrese the nose-down pitching moment by 70% without a force loss.

• The Transactions of the Korean Institute of Electrical Engineers A
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• v.49 no.7
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• pp.365-368
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• 2000

Traditionally, load flows have been calculated using the Gauss-Seidel and Newton-Raphson Method. DistFlow Method which is proposed by Wu and Baran is superior to the other two methods because it does not require the admittance matrix calculation to optimize the distribution system. This paper introduces a new alternative algorithm to the DistFlow Method which is slow and complex to find solutions as the number of lateral and sublateral increases. The proposed load flow method can construct System Jacobian easily. We can minimize the off-diagonal elements of the branch Jacobian and submatrices in the System Jacobian. Simulation results show that progressive performances of the proposed algorithm have a better convergence time.

• Journal of Food Hygiene and Safety
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• v.28 no.3
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• pp.213-216
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• 2013

Antibiotic Detection Kit (Combination I), a lateral flow immunoassay (LFIA) developed for the detection of antibiotic residues in milk, was utilized for the analysis of antibiotic residues in the muscle tissue of olive flounder. After 60-min treatment by dipping in water dosed with ampicillin (200-g/ton water), the residue depletion of ampicillin was investigated in 25 cultured olive flounder (Paralichthys olivaceus). Muscles of fish were sampled on the 1st, 2nd, 3rd, 4th and 5th day after drug treatment. The concentration of ampicillin in the muscle was determined by LFIA. The absorbance ratio of the sample to the control blank (Bs/Bo) was employed as an index to determine the muscle residues in olive flounder. To investigate the recovery rate, standard solutions were added to muscle samples to give final concentrations in the muscle of 4 and 8 ng/ml. The recovery rates of all spiked samples were > 96% of the spiked value. Ampicillin was detected in the muscle of fish treated with the drug until the 2nd day of the withdrawal period. The present study showed that the LFIA can be easily adopted to predict ampicillin residues in tissue of farmed fishes.

• Parasites, Hosts and Diseases
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• v.54 no.3
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• pp.375-380
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• 2016

Angiostrongyliasis is difficult to be diagnosed for the reason that no ideal method can be used. Serologic tests require specific equipment and are not always available in poverty-stricken zone and are time-consuming. A lateral flow immunoassay (LFIA) may be useful for angiostrongyliasis control. We established a LFIA for the diagnosis of angiostrongyliasis based on 2 monoclonal antibodies (mAbs) against antigens of Angiostrongylus cantonensis adults. The sensitivity and specificity were 91.1% and 100% in LFIA, while those of commercial ELISA kit was 97.8% and 86.3%, respectively. Youden index was 0.91 in LFIA and 0.84 in commercial ELISA kit. LFIA showed detection limit of 1 ng/ml of A. cantonensis ES antigens. This LFIA was simple, rapid, highly sensitive and specific, which opened an alternative approach for the diagnosis of human angiostrongyliasis.

• Journal of Korea Water Resources Association
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• v.40 no.3
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• pp.277-286
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• 2007

In this study, we analyzed the flow characteristics in the wide-crested side weir of trapezoidal section by using a three dimensional numerical stimulation. From this study, as the Froude number increases in the main channel, the overflow discharge ratio and the discharge coefficient of lateral overflow tend to decrease. And it was also found that the increase of the lateral overflow reduces the channel discharge area in the downstream, and the size of recirculating zone is increasing in the opposite side of side weir. The stream-wise water surface on the side where the side weir is installed falls down rapidly in the weir starting point, gradually ascending, and rapidly rising at the end point. The reason why the water surface rapidly rises at the weir end point is because the weir end point hinders the flow.

• International Journal of Oral Biology
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• v.44 no.1
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• pp.8-13
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• 2019

Recently, the importance of on-site detection of pathogens has drawn attention in the field of molecular diagnostics. Unlike in a laboratory environment, on-site detection of pathogens is performed under limited resources. In this study, we tried to optimize the experimental conditions for on-site detection of pathogens using a combination of ultra-fast convection polymerase chain reaction (cPCR), which does not require regular electricity, and nucleic acid lateral flow (NALF) immunoassay. Salmonella species was used as the model pathogen. DNA was amplified within 21 minutes (equivalent to 30 cycles of polymerase chain reaction) using ultra-fast cPCR, and the amplified DNA was detected within approximately 5 minutes using NALF immunoassay with nucleic acid detection (NAD) cassettes. In order to avoid false-positive results with NAD cassettes, we reduced the primer concentration or ultra-fast cPCR run time. For singleplex ultra-fast cPCR, the primer concentration needed to be lowered to $3{\mu}M$ or the run time needed to be reduced to 14 minutes. For duplex ultra-fast cPCR, $2{\mu}M$ of each primer set needed to be used or the run time needed to be reduced to 14 minutes. Under the conditions optimized in this study, the combination of ultra-fast cPCR and NALF immunoassay can be applied to on-site detection of pathogens. The combination can be easily applied to the detection of oral pathogens.

• Journal of Microbiology and Biotechnology
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• v.29 no.3
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• pp.454-464
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• 2019

Salmonellosis is a highly contagious bacterial disease that threatens both human and poultry health. Tests that can detect Salmonella in the field are urgently required to facilitate disease control and for epidemiological investigations. Here, we combined loop-mediated isothermal amplification (LAMP) with a chromatographic lateral flow dipstick (LFD) to rapidly and accurately detect Salmonella. LAMP primers were designed to target the Salmonella invA gene. LAMP conditions were optimized by adjusting the ratio of inner to outer primers, $MgSO_4$ concentration, dNTP mix concentration, amplification temperature, and amplification time. We evaluated the specificity of our novel LAMP-LFD method using six Salmonella species and six related non-Salmonella strains. All six of the Salmonella strains, but none of the non-Salmonella strains, were amplified. LAMP-LFD was sensitive enough to detect concentrations of Salmonella enterica subsp. enterica serovar Pullorum genomic DNA as low as $89fg/{\mu}l$, which is 1,000 times more sensitive than conventional PCR. When artificially contaminated feed samples were analyzed, LAMP-LFD was also more sensitive than PCR. Finally, LAMP-LFD gave no false positives across 350 chicken anal swabs. Therefore, our novel LAMP-LFD assay was highly sensitive, specific, convenient, and fast, making it a valuable tool for the early diagnosis and monitoring of Salmonella infection in chickens.