• 제목/요약/키워드: Laser scanning

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Digital Documentation and Short-term Monitoring on Original Rampart Wall of the Gyejoksanseong Fortress in Daejeon, Korea (대전 계족산성 원형성벽의 디지털기록화 및 단기모니터링 연구)

  • Kim, Sung Han;Lee, Chan Hee;Jo, Young Hoon
    • Economic and Environmental Geology
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    • v.52 no.2
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    • pp.169-188
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    • 2019
  • This study was carried out unmanned aerial photography and terrestrial laser scanning to establish digital database on original wall of Gyejoksanseong fortress, and measured ground control points for continuity of the monitoring. It also performed precise examination with the naked eye, unmanned aerial photogrammetry, endoscopy, total station and handy measurement to examine the structural stability of the original walls. The ground control points were considered as a point where visual field can be secured, 3 points were selected around each of the south and north walls. For the right side of the south original wall, aerial photogrammetry was conducted using drones and a deviation analysis of 3-dimensional digital models was performed for short-term monitoring. As a result, the two original walls were almost matched in range within 5mm, and no difference indicating displacement of stones was found, except for partial deviation. Regular monitoring of the areas with structural deformation such as bulging, weak and fracture zone by precisely examining with the naked eye and using high-resolution photo data revealed no distinct change. The inner foundation observed through endoscopy found out that filling stones of the original walls were still remained, while most filling soil was lost. As a result of measuring the total station focusing around the points with structural deformation on the original walls, the maximum displacements of the north and south walls were somewhat high with 6.6mm and 3.8mm, respectively, while the final displacements were relatively stable at below 2.9mm and 1.4mm, respectively. Handy measurement also did not reveal clear structural deformation with displacements below 0.82mm at all points. Even though the results of displacement monitoring on the original walls are stable, it is hard to secure structural stability due to the characteristics of ramparts where sudden brittle fracture occurs. Therefore, it is necessary to conduct conservational scientific diagnosis, precise monitoring, and structural analysis based on the 3-dimensional figuration information obtained in this research.

Effect of Prostaglandin F2 Alpha on E-cadherin, N-cadherin and Cell Adhesion in Ovarian Luteal Theca Cells (난소의 황체협막세포에서 E-cadherin, N-cadherin과 세포부착에 미치는 Prostaglandin F2 Alpha의 영향)

  • Lee, Sang-Hee;Jung, Bae Dong;Lee, Seunghyung
    • Korean Journal of Clinical Laboratory Science
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    • v.51 no.3
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    • pp.360-369
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    • 2019
  • Cadherins are essential transmembrane proteins that promote cell-cell adhesion and maintain the corpus luteum structure in the ovary. This study examined the influence of prostaglandin F2 alpha ($PGF2{\alpha}$) on E-cadherin, N-cadherin, and adhesion in luteal theca cells (LTCs). The luteal cells were isolated from the mid-phase corpus luteum, and the LTCs were cultured separately from the luteal heterogeneous cells according to the morphology of the mesenchymal cells and to determine if steroidogenic and endothelial cells of LTCs, 3beta-hydroxysteroid dehydrogenase ($3{\beta}$-HSD), and vascular endothelial growth factor receptor 2 (VEGFR2) mRNA were used. The LTCs were then incubated in the culture medium supplemented with 0.01, 0.1, and 1.0 mM $PGF2{\alpha}$ for 24 h, and the E-cadherin and N-cadherin proteins in the LTCs were detected by confocal laser scanning microscopy. The results revealed $3{\beta}$-HSD mRNA expression in the LTC but no VEGF2R mRNA expression. The E-cadherin and N-cadherin proteins of the LTCs were damaged in the 0.01, 0.1, and 1.0 mM $PGF2{\alpha}$ treatment groups, and the expression of the N-cadherin protein was reduced significantly in 0.01 mM $PGF2{\alpha}$ compared to the 0 mM $PGF2{\alpha}$ treatment groups (P<0.05). In addition, the number of attached LTCs were significantly lower in the 0.01 mM $PGF2{\alpha}$ treatment group than in the 0 mM $PGF2{\alpha}$ treatment group (P<0.05). In conclusion, $PGF2{\alpha}$ affected the disruption of cadherin proteins and cell adhesion in LTCs. These results may help better understand the cadherin and adhesion mechanism during corpus luteum regression in the ovary.

Emulsifying Properties of Gelatinized Octenyl Succinic Anhydride Modified starch from Barley (호화 옥테닐 호박산 전분의 유화 특성)

  • Kim, San-Seong;Kim, Sun-Hyung;Lee, Eui-Seok;Lee, Ki-Teak;Hong, Soon-Taek
    • Journal of the Korean Applied Science and Technology
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    • v.36 no.1
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    • pp.174-188
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    • 2019
  • The present study was carried out to investigate the emulsifying properties of heat-treated octenyl succinic anhydride(OSA) starch and the interfacial structure at oil droplet surface in emulsions stabilized by heat-treated OSA starch. First, the aqueous suspensions of OSA starch were heated at $80^{\circ}C$ for 30 min. Oil-in-water emulsions were then prepared with the heat-treated OSA starch suspension as sole emulsifier and their physicochemical properties such as fat globule size, surface load, zeta-potential, dispersion stability, confocal laser scanning microscopic image(CLSM) were determined. It was found that fat globule size decreased as the concentration of OSA starch in emulsions increased, showing a lower limit value ($d_{32}:0.31{\mu}m$) at ${\geq}0.2wt%$. Surface load increased steadily with increasing OSA starch concentration in emulsions, possibly forming multiple layers. In addition, fat globule sizes were also influenced by pH: they were increased in acidic conditions and these results were interpreted in view of the change in zeta potentials. The dispersion stability by Turbiscan showed that it was more unstable in emulsions at acidic condition. Heat-treated OSA starch found to adsorb at the oil droplet surface as some forms of membrane (not starch granules), which might be indicative of stabilizing mechanism of OSA starch emulsions to be steric forces.

Methylene Blue Photodegradation Properties of Anatase/brookite Hybrid TiO2 Photocatalyst Prepared with Different Acid Catalysts (산 촉매에 따른 아나타제/브루카이트 혼성형 TiO2 광촉매의 메틸렌블루 광분해특성)

  • Yun, Seok-Min;Kim, Jinhoon;Jeong, Euigyung;Im, Ji Sun;Lee, Young-Seak
    • Applied Chemistry for Engineering
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    • v.22 no.1
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    • pp.21-25
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    • 2011
  • In this study, anatase/brookite hybrid $TiO_2$ was prepared using different acid catalysts and microwave to improve photodegradation of organic pollutants. The methylene blue photodegradation properties of the prepared photocatalysts with different particle/crystal size and brookite fractions were investigated. Surface characteristics and particle sizes of anatase/brookite hybrid $TiO_2$ were evaluated using scanning electron microscopy (SEM) and laser diffraction particle size analyzer, respectively and crystal structures were investigated with X-ray diffraction (XRD). Methylene blue photodegradation properties were evaluated with UV-vis spectrophotometer. Anatase and anatase/brookite hybrid $TiO_2$ had less than 500 nm size of clusters and the average particle size of $6.66{\sim}6.85{\mu}m$, suggesting that types of acid catalysts did not affect the size. XRD of the prepared $TiO_2$ showed that the photocatalysts had anatase/brookite hybrid crystal structure and applying microwave did not change their crystal structure. Photodegradation of methylene blue with the prepared photocatalyst did not increased proportionally to the fraction of brookite and the crystal size and decreased when brookite fraction and the crystal size increased further. Anatase/brookite hybrid $TiO_2$ with brookite fraction of 9.4% and crystal size of 4.53 nm shows the best photodegradation activity of methylene blue.