• 한국양식학회지
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• 제20권4호
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• pp.239-247
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• 2007

관상가치와 교재생물로서 교육적인 이용도가 높은 흰동가리속 red and black clownfish Amphiprion melanopus의 인공종묘생산의 기초자료를 확보하기 위하여 실험실적인 규모에서 친어를 사육하여 산란과 부화를 조사하였다. 한마리의 암컷이 2004년 4월 19일 부터 2007년 4월 16일 까지 총 48번의 산란을 하였으며, 산란 직후의 수정란의 장경은 $2.6{\sim}2.15\;mm$ (평균$2.32{\pm}0.11\;mm$, n=50), 단경은 $0.94{\sim}0.96\;mm$ ($0.94{\pm}0.001\;mm$, n=50)로 타원형이다. 수정란은 유백색으로 유구를 많이 가진 상태로 난막에 쌓여 배체의 동물극 쪽이 부착사에 의해 산란기질에 부착되어 있었다. 산란상에 대한 선택성은 red clay pot > ceramic pot > volcanic scoria > live rock으로 나타났으며, 부화율은 red clay pot에서 66%로 live rock 34%에 비하여 유의적으로 높았다(P<0.05). 먹이종류에 대한 산란량은 Tetra bits+바지락육질+진주담치육질+astaxanthin을 혼합하여 먹인 실험구에서 $638{\pm}16.04$개로 Tetra bits만 공급한 구간 $477{\pm}28.96$ 개와 비교하여 유의적으로 높게 나타났다(P<0.05). 부화를 위한 배발생의 최적 환경요인은 수온 $28^{\circ}C$, 염분 33 psu, 광주기 6L:18D로 나타났다. 갓 부화한 자어의 전장은 $3.7{\sim}4.8\;mm$ (평균 $4.6{\pm}0.14\;mm$, n=50)로 7일째 까지는 $7.5{\pm}0.60\;mm$, 14일까지는 $11.0{\pm}0.77\;mm$, 20일에는 $12.4{\pm}0.85\;mm$, 30일에는 $18.8{\pm}0.82\;mm$, 70일에는 $37.9{\pm}2.26\;mm$로 성장하였다.

• 한국해양학회지:바다
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• 제13권2호
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• pp.140-146
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• 2008

해양생태독성평가를 위한 표준시험방법 개발을 위하여 해양생태계의 대표 소비자인 어류를 이용한 시험방법을 정립하였다. 표준시험생물은 송사리(Oryzias latipes)와 넙치(Paralichthys olivaceus)를 선정하였으며, endpoint는 7일 자어 사망률(7 day-$LC_{50}$)로 설정하였다. 표준시험방법은 미환경보호국(USEPA, 1994)의 어류독성시험법을 참고하였으나, 시험생물은 생태적 대표성 및 종의 유용성 등을 고려하여 재설정하였다. 송사리는 실험실 사육이 가능하고 광염성이며, 넙치는 국내 연안 생태계의 대표 어류이자 상업용 종묘 생산 시설을 통하여 연중 시험생물 확보가 가능한 점을 고려하여 선정하였다. 염분 내성 및 표준물질독성실험결과, 송사리는 $0{\sim}35\;psu$ 구간에서 전개체가 생존하였으며, 넙치는 염분이 20 psu 이상에서만 실험이 가능하였다. 독성시험기간은 7일이며, 시험구내의 용존산소가 4mg/L 이상을 유지하는 한 시험용액의 교환없이 수행하는 비교환정수방식을 택하였다. 시험생물의 연령은 부화 후 초기 사망률과 시험시 취급이 용이한 크기로 선정하였으며, 송사리의 경우 부화 후 7일(전장 약 5 mm), 넙치의 경우 25일(전장 약 10 mm)로 선정하였다. 시험적합도 기준은 대조구에서의 생존율이 80% 이상으로 설정하였으며, 표준독성물질에 의한 민감도를 어류 독성 시험시 동일한 방법을 이용한 결과를 제시하도록 하였다.

• 한국발생생물학회지:발생과생식
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• 제17권4호
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• pp.399-407
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• 2013

Morphological changes in the reared rock bream, Oplegnathus fasciatus, from hatching to six days after hatching were examined during the early growth stage under starvation. All the larvae died within five days when feeding was delayed for three days after hatching. These results imply that initial larval food should be supplied within two days of hatching. Changes in the pectoral angle and the ratios of eye height to head height, gut height to standard length, and gut height to myotome height in the rock bream are alternative indicators for the identification of starving fish. These indicators might prove useful in evaluating the successful transition from endogenous to exogenous feeding in this species.

• International Journal of Industrial Entomology and Biomaterials
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• 제21권2호
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• pp.185-187
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• 2010

Most studies of the black soldier fly, Hermitia illucens, widely distributed throughout Southeast Asia and America focused on the use of larvae for recycling various organic materials and feed for fish and livestock. To recognize the instars of the soldier fly's larvae, we examined the number of exuviae originated from each larva from 1st instar to pupa. The weight and the head capsule width of the black soldier fly larvae also confirmed that the black soldier fly was passed through 6th instars. In addition, we found that the black soldier fly larvae had a well developed mandibular-maxillary complex, similar to those reported from the larvae of scavengers including Odontomyia cincta, Neopachygaster maculicornis, Ptecticus brunettii and Stratiomys ruficornis.

• 한국어병학회지
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• 제16권1호
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• pp.13-21
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• 2003

The infection characteristics with scuticociliates at on-land rearing farms and hatcheries of flounder, Paralithys olivaceus was investigated during the year of 2001 by juvenile infection routes. When culture tanks for living food organisms such as chlorella, rotifer, and Artemia were searched, scuticocilates were detected both in live and dead rotifer, and at the dregs of culture tank bottoms at almost hatcheries. When rotifer infected with scuticocilates fed on fish larvae, lots of scuticocilate were inhabited at the bottom of fry rearing tanks. After feeding on scuticocilates-infected rotifer on fish larvae, first infection was detected at 10 days after bottom dwelling or 40 days old after hatching. By histopathological examination we confirmed the infection route of eyeball or brain contamination was that the ciliate worms digged through mouth and front part of the dosal fin cuticle, transferred into eyeball along the epithelium and muscle tissue, and reached finally into brain by the muscle and nerve tissue. The infection of internal organs was clarified into two routes. The first route was started from the infection at ventral and anal fin rays by the worms, and reached at the anus and rectum through the epithelium and muscle tissue. The second route was initiated from the infection at urinary organ and reached into the rectum epithelium cells, inner wall of intestine, abdominal cavity, pancreas, kidney, and pancreas. At seed production farms where fish larvae fed on scuticocilate-free rotifer, the worms were not detected not only at the food organisms culture tanks and juvenile rearing tanks but also larval flounder less than 7cm in total length.

• 대한수의학회지
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• 제48권4호
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• pp.473-480
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• 2008

A high incidence of Anisakiasis has been reported in many countries where people eat frequently raw or undercooked seafood. Anisakis spp. larvae were obtained from the mackerel acquired from a fish market of Cheongju city. They were divided into several groups and placed in culture dishes containing RPMI-1640 (culture media), in the presence or absence of different concentrations of herbal extracts (Meliae ezadarach, Dryopteris crassirhizoma, Quisqualis indica var villosa). The objective of the present study was to investigate the activity of larval migration inhibition in vitro. Meliae ezadarach at the concentrations of 7.5, 15, and 30 mg/ml effectively inhibited the larvae migration in time-dependent manner during experimental period of 0-24 h. Treatment of Meliae ezadarach at the three concentrations completely inhibited the larvae migration in vitro. Dryopteris crassirhizoma at the concentrations of 5, 10, and 20 mg/ml also effectively inhibited the larvae migration in a time-dependent manner. The treatment of Dryopteris crassirhizoma for 12 h completely inhibited the larvae migration. The inhibitory effect of Dryopteris crassirhizoma was stronger than that of Meliae ezadarach. Although Quisqualis indica var villosa also showed the inhibitory effect on larvae migration, its inhibitory efficacy was the weakest among tested herbal extracts. These results indicated that some herbal extracts may be useful in controlling human anisakiasis.

• 한국수산과학회지
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• 제49권2호
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• pp.154-160
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• 2016

Several our studies have focused recently on the mass production of the freshwater Cladoceran Moina macrocopa which can substitute Artemia nauplii for the culture of larval marin fish. A 6 weeks experiment was conducted to investigate the effects of enrichment on the fatty acid composition of Moina macrocopa through feeding Schizochytrium sp. containing highly unsaturated fatty acids and to study the impacts of n-3-HUFA enriched Moina on improving survival rate and fatty acid compostion of larval rockfish Sebastes schlegeli. After feeding for 6 weeks, the Moina-fed fry resulted in a higher survival rate of 99.2% compared to the Artemia-fed fry 12.8%. In addition, the Moina-fed fry had the fast growth rate 45.6mm compare to the Artemia-fed fry 25.7 mm at the end of the experiment. The Moina-fed fry showed significantly higher level of 16.47% DHA than their Artemia-fed fry counterparts of the level of 3.97% with respect to DHA. PL, the cell membrane components in living food organisms, constituted 63.8% of the Moina, which was significantly higher than in the 40.1% of the Artemia. The present study indicate that Moina macrocopa can be used as Artemia substitute and improving the survival rate rockfish larvae through enchriment Schizochytrium sp.

• 생태와환경
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• 제47권2호
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• pp.82-90
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• 2014

본 연구에서는 부유토사로 인한 탁도 증가가 어류에게 미치는 영향에 대해 실험실 모의실험을 수행하였다. 국내 강 또는 호소에서 출현 비율이 높은 붕어, 잉어, 송사리를 대상으로 수정란 부화율 평가를 수행한 결과 붕어, 잉어는 4,040 NTU 수준까지 수정란 부화에 미치는 영향이 없었지만, 송사리는 254 NTU 이상의 구간에서 부화율이 저해되는 영향을 보였다. 붕어, 잉어, 송사리 치어의 4일간 노출 결과 생존에 미치는 영향은 없었다. 반면 성장률 결과에서 잉어와 붕어는 7일, 14일, 21일 모두 143 NTU 구간부터 성장저해 영향을 보였다. 송사리는 14일 노출 시 254 NTU, 21일간 노출 시 501 NTU 구간부터 성장저해 영향을 보였다. 따라서 강 또는 호소에서 143 NTU 이상의 탁도가 발생할 경우 붕어, 잉어의 성장을 저해시킬 수 있고, 250 NTU 이상의 탁도는 송사리와 같이 민감 어종의 부화율이 저해될 수 있을 것으로 사료된다.

• 한국어류학회지
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• 제28권3호
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• pp.192-199
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• 2016

조류에 의한 에너지가 상대적으로 매우 큰 한강 하구역의 대표적인 석모수로와 염하수로에서 2007년 8월과 2008년 8월에 연속 관측을 통해 조석주기에 따른 소하성 어종인 웅어속 자치어의 출현량을 조사하였다. 조석주기에 따른 환경측정 결과, $1^{\circ}C$ 미만의 수온차를 보였고, 염분은 창조 시 최고 15.1 psu, 낙조 시 최저 0.8 psu로 조석에 따른 염분차가 컸다. 웅어속 자치어는 창조 시보다 낙조 시 높은 출현량을 보였다 (p<0.05). 이는 소하성 어종인 웅어속 자치어가 한강 상류 또는 중류에서 부화한 후, 보육장인 하구역에 정착하기 위해 수평이동 선택적 조수흐름 (STST)으로 낙조류를 이용하는 것으로 사료되며, 특히 낙조 시 13.6 mm 이상의 유영력이 향상된 개체일수록 뚜렷하게 나타났다.

• 한국수산과학회지
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• 제49권1호
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• pp.38-44
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• 2016

The quality and quantity of live food sources strongly influence the success of fish production in farming operations. Thus, critical studies of live forage species are a crucial element for progress in fish aquaculture. The fat content of food is an especially important determinant of growth in marine fish. Omega-3 highly unsaturated fatty acids (HUFA) are essential components of diet that determine the nutritional value of larval fish. Euglena is a protist that has potential as a forage species. These single-celled organisms have plant and animal characteristics they are motile, elliptical in shape and 15–500 μm in diameter. Their nutritional content is excellent, but most studies have focused on cells raised in autotrophic culture. We therefore examined differences in the lipid and fatty acid contents, and the growth of Euglena cells grown under autotrophic, heterotrophic, and mixotrophic conditions. Biomass production reached 15.03 g/L, 12.28 g/L, and 3.66 g/L under mixotrophy, heterotrophy, and autotrophy, respectively. The proportional n-3 HUFA content differed among culture methods: 10.04%, 5.80% and 10.01% in mixotrophic, heterotrophic and autotrophic cultures, respectively. Mixotrophy was to be the best form of cultivation for improving the growth and nutritional content of Euglena.