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Inhibitory effects of herbal extracts (Meliae ezadarach, Dryopteris crassirhizoma, Quisqualis indica var villosa) on larval migration of Anisakis spp. in vitro  

Kwon, Hee-Nyung (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
Jee, Cha-Ho (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
Publication Information
Korean Journal of Veterinary Research / v.48, no.4, 2008 , pp. 473-480 More about this Journal
Abstract
A high incidence of Anisakiasis has been reported in many countries where people eat frequently raw or undercooked seafood. Anisakis spp. larvae were obtained from the mackerel acquired from a fish market of Cheongju city. They were divided into several groups and placed in culture dishes containing RPMI-1640 (culture media), in the presence or absence of different concentrations of herbal extracts (Meliae ezadarach, Dryopteris crassirhizoma, Quisqualis indica var villosa). The objective of the present study was to investigate the activity of larval migration inhibition in vitro. Meliae ezadarach at the concentrations of 7.5, 15, and 30 mg/ml effectively inhibited the larvae migration in time-dependent manner during experimental period of 0-24 h. Treatment of Meliae ezadarach at the three concentrations completely inhibited the larvae migration in vitro. Dryopteris crassirhizoma at the concentrations of 5, 10, and 20 mg/ml also effectively inhibited the larvae migration in a time-dependent manner. The treatment of Dryopteris crassirhizoma for 12 h completely inhibited the larvae migration. The inhibitory effect of Dryopteris crassirhizoma was stronger than that of Meliae ezadarach. Although Quisqualis indica var villosa also showed the inhibitory effect on larvae migration, its inhibitory efficacy was the weakest among tested herbal extracts. These results indicated that some herbal extracts may be useful in controlling human anisakiasis.
Keywords
Anisakiasis; Dryopteris crassirhizoma; larval migration inhibition; Meliae ezadarach; Quisqualis indica var villosa;
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