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Establishment of Marine Ecotoxicological Standard Method for Larval Fish Survival Test  

Park, Gyung-Soo (Department of Marine Biotechnology, Anyang University)
Kang, Ju-Chan (Department of Aquatic Life Medicine, Pukyong National University)
Yoon, Sung-Jin (Korea Institute of Toxicology)
Lee, Seung-Min (Marine Environment Division of West Sea Fisheries Research Institute)
Hwang, Un-Ki (Marine Environment Division of West Sea Fisheries Research Institute)
Publication Information
The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY / v.13, no.2, 2008 , pp. 140-146 More about this Journal
Abstract
Marine ecotoxicological standard method using fish larvae was established with the standard test species of Oryzias latipes(Japanese Medaka) and Paralichthys olivaceus(flounder) and with the 7 day $LC_{50}$ as endpoint. Test method referred to the USEPA(1994) with the replacement of test species found in the Korean water. Standard test species were selected in terms of the species supply and ecological importance in Korean waters. Japanese medaka can be reared with small tanks in the lab and has wide tolerance on salinity, and flounder eggs can be easily obtained from commercial fish hatcheries. General conditions for larval fish toxicity test are as follows. The possible salinity ranges for toxicity test were $0{\sim}35\;psu$ for medaka and >20 psu for flounder. Test type was designated as static non-renewal test if the dissolved oxygen in the test chamber does not fall below 4.0 mg/L. Ages of test species were selected as 7 days after hatched for medaka(about 5 mm TL) and 25 days for flounder(about 10 mm TL) because of the low natural mortality after these periods. Test can be accepted when the survival rates are over 80% in control. Also, species sensitivity on standard reference materials(copper, cadmium or zinc) must be provided with the toxicity test results.
Keywords
Standard Method; Toxicity; Oryzias latipes; Paralichthys olivaceus$LC_{50}$;
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Times Cited By KSCI : 1  (Citation Analysis)
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