• The Plant Pathology Journal
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• v.36 no.5
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• pp.509-514
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• 2020

Satsuma dwarf virus (SDV) seriously damages citrus production by reducing the quality and yield of fruit. To avoid contamination with SDV, mother trees are checked to be SDV-free in advance of nursery tree distribution. In this study, we compared an immunochromatographic assay (ICA) kit with double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for large-scale diagnosis of SDV in orchardgrown trees in Shizuoka Prefecture, Japan. The two methods gave conflicting results for 11 of 1,705 samples, all of which were negative by DAS-ELISA but positive by ICA. The samples scored as positive by either DAS-ELISA or ICA were analyzed by reverse transcription polymerase chain reaction and all were confirmed to be positive. These results validate the use of ICA as a screening method for large-scale diagnosis. Strain discrimination revealed that 16 of 22 isolates belonged to SDV, while citrus mosaic virus (CiMV) infection only and co-infection (SDV and CiMV) were in a minority.

• Molecules and Cells
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• v.44 no.7
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• pp.500-516
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• 2021

Cardiac hypertrophic signaling cascades resulting in heart failure diseases are mediated by protein phosphorylation. Recent developments in mass spectrometry-based phosphoproteomics have led to the identification of thousands of differentially phosphorylated proteins and their phosphorylation sites. However, functional studies of these differentially phosphorylated proteins have not been conducted in a large-scale or high-throughput manner due to a lack of methods capable of revealing the functional relevance of each phosphorylation site. In this study, an integrated approach combining quantitative phosphoproteomics and cell-based functional screening using phosphorylation competition peptides was developed. A pathological cardiac hypertrophy model, junctate-1 transgenic mice and control mice, were analyzed using label-free quantitative phosphoproteomics to identify differentially phosphorylated proteins and sites. A cell-based functional assay system measuring hypertrophic cell growth of neonatal rat ventricle cardiomyocytes (NRVMs) following phenylephrine treatment was applied, and changes in phosphorylation of individual differentially phosphorylated sites were induced by incorporation of phosphorylation competition peptides conjugated with cell-penetrating peptides. Cell-based functional screening against 18 selected phosphorylation sites identified three phosphorylation sites (Ser-98, Ser-179 of Ldb3, and Ser-1146 of palladin) displaying near-complete inhibition of cardiac hypertrophic growth of NRVMs. Changes in phosphorylation levels of Ser-98 and Ser-179 in Ldb3 were further confirmed in NRVMs and other pathological/physiological hypertrophy models, including transverse aortic constriction and swimming models, using site-specific phospho-antibodies. Our integrated approach can be used to identify functionally important phosphorylation sites among differentially phosphorylated sites, and unlike conventional approaches, it is easily applicable for large-scale and/or high-throughput analyses.

• Journal of the Korean Operations Research and Management Science Society
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• v.5 no.1
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• pp.89-90
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• 1980

원래 다량자료의 정리(Large Scale Data Screening)는 어떤 정립된 이론에 의해 수행되는 것보다는 그 자료자체가 가지는 성격과 자원의 Availability 등을 고려하여 수행되는 것이 상례이다. 여기서는 여러 방법 중 자료가 모두 수치로 나타나는 경우 자료정리의 한 유용한 방법으로 회귀분석을 사용하는 방법에 대해 설명코자 한다.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.45 no.3
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• pp.139-149
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• 2022

Korea's Corona 19(COVID-19) quarantine, referred to as 'K-Quarantine', is a globally recognized quarantine system that has achieved both conflicting goals: health and economy. The quarantine system represented by 3T(Test-Trace-Treat) is not a method of blocking an area, but a method of screening and treating infected and non-infected persons. The screening center, one of the key elements of this screening treatment system, has evolved to suit the timing and situation of COVID-19, and has succeeded in initial response by conducting large-scale tests quickly and safely. By analyzing the evolution of screening centers that produced such significant results from a problem-solving point of view, it proved its meaning as a practical success case of creative problem-solving. In addition, the usefulness of TRIZ (Russian abbreviation of Theory of Solving Inventive Problem), a creative problem-solving theory, was confirmed through an analysis of actual verified cases of COVID-19 response. TRIZ is a problem-solving theory created by analyzing the regularity of invention patents, and is widely used not only in the technical field but also in the non-technical fields such as design, management, and education. The results of this study are expected to provide useful meaning and practical examples to researchers interested in system analysis and TRIZ application from a problem-solving perspective.

• Journal of the Korean Society of Clothing and Textiles
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• v.28 no.3_4
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• pp.396-402
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• 2004

The purpose of this study was to investigate the difference of cotton fabric texture perceived between on-line(screening fabric) and off-line(real fabric), and to analyze fabric characteristics having an effect on the difference. The similarity of 55 various cotton fabrics perceived between on-line and on-line were measured showing simultaneously real fabrics and screening fabrics by 7-scale questionnaire. And the characteristics of cotton fabrics such as weave structure, thickness, weight, fabric density, stiffness, Hunter's L, a, b, and hue were measured. Cotton fabrics were classified into 3 groups by extent of similarity. There were no significant differences in weft density, stiffness, Hunter's L, a, b, and hue among 3 groups. But there were significant differences in weave structure, thickness, weight, warp density, and difference of warp ＆ weft density. The fabrics having large similarity were thick and heavy, had small warp density and difference of warp ＆ weft density, and distinct surface texture. The group having medium similarity included fabrics of medium thickness and weight, having weak surface texture, large warp density and difference of warp ＆ weft density. The group having small similarity, which the differences between on-line and off-line were large, included thin and light fabrics having smooth surface and large warp density and difference of warp ＆ weft density.

• Progress in Superconductivity and Cryogenics
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• v.2 no.1
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• pp.31-34
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• 2000

this paper deals with the initial magnetic field decay for a large scale superconducting magnet e.g. NMR/MRI magnet. The high resolution image can not be obained during the periods of the initial field decay. It is known that all superconducting materials have the property of diamagnetism. This diamagnetism is usually explained with the concept of screening current. We assumed that the existence of the screening currebt. we assumed that the existence of the screening current makes the current distribution in the superconducting wire non-uniform. And the initial magnetic field decay is caused steady current state in the view of its pattern. The initial magnetic field decay is caused by the change of the current distribution between the energizing state and persistent current mode. in this paper the theoretical analysis for the current distributions has been introduced for each state. The experiments have been carried out to verify transport currents in order to veperiments, it small at the higher transport current.

• Proceedings of the Technology Innovation Conference
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• 2003.06a
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• pp.3-11
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• 2003

As the size and scope of R&D investment explodes, the strategic and managerial importance of R&D proposal screening becomes highlighted. This point is particularly true for a large-scale research center that deals with multi-product and multi-technology R&D projects. Despite the importance, however, previous research has focused on project evaluation and selection stage. In this research, we propose a R&D proposal screening system. The main objective of the system is to filter R&D proposals that are identified to be duplications of past or existing projects. To this end, the algorithm of the system employs text mining, multivariate statistical method, and case-based reasoning.

• Journal of KIISE:Computer Systems and Theory
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• v.35 no.6
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• pp.237-247
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• 2008

A virtual screening is the process of reducing an unmanageable number of compounds to a limited number of compounds for the target of interest by means of computational techniques such as molecular docking. And it is one of a large-scale scientific application that requires large computing power and data storage capability. Previous applications or softwares for molecular docking such as AutoDock, FlexX, Glide, DOCK, LigandFit, ViSION were developed to be run on a supercomputer, a workstation, or a cluster-computer. However the virtual screening using a supercomputer has a problem that a supercomputer is very expensive and the virtual screening using a workstation or a cluster-computer requires a long execution time. Thus we propose a service-based virtual screening system using Grid computing technology which supports a large data intensive operation. We constructed 3-dimensional chemical molecular database for virtual screening. And we designed a resource broker and a data broker for supporting efficient molecular docking service and proposed various services for virtual screening. We implemented service based virtual screening system with DOCK 5.0 and Globus 3.2 toolkit. Our system can reduce a timeline and cost of drug or new material design.

• The Plant Pathology Journal
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• v.21 no.3
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• pp.288-292
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• 2005

Pathogenesis-related protein-1a (PR-1a) is strongly induced in tobacco plants by pathogen attack, exogenous salicylic acid (SA) application and by other developmental processes. In order to develop a rapid screening system for the selection of plant defense-inducing compounds originated from various sources, we have transformed tobacco Samsun NN plants with a chimeric construct consisting of GUS $(\beta-glucuronidase)$. In the $T_1$ generation, three transgenic lines having stable GUS expression were selected for further promoter analysis. Using GUS histochemical assay, we observed strong GUS induction driven by PR-1a promoter in PR1a-GUS transgenic tobacco leaves in response to the exogenous application of SA or benzol (1,2,3) thiadiazole-7-carbothioic acid S-methyl ester (BTH), a SA­derivative compound. In addition, GUS expression was maintained locally or systemically in PR1a-GUS transgenic line $\#5\;T_2$ generation) until after 3 days when they were treated with same chemicals. Our results suggested that the PR1a-GUS reporter gene system in tobacco plants may be applicable for the large-scale screening of defense-inducing substances.

• 한국디지털정책학회:학술대회논문집
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• 2006.06a
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• pp.289-297
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• 2006

Business operates under the fast pace of large-scale changes. These changes occur in environments including competitors, markets and consumers. To survive under these changes, process innovation is becoming the eminent issue faced by executives. Process innovation is to generate, select, and implement new ideas adopting changes in organizational structure, management pattern, human resource management systems and so on. Examples include restructuring, outsourcing, benchmarking, total quality management, and business process reengineering. This study suggests how to develop process innovation ideas. Related screening and implementation techniques are also presented.