• Asian-Australasian Journal of Animal Sciences
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• 제30권11호
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• pp.1651-1659
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• 2017

Objective: Lao PDR's recent accession to the World Trade Organization necessitates a greater understanding of the patterns and risk of livestock production in order to better align national policy with the Agreement on the Application of Sanitary and Phytosanitary Measures. This eco-health study was conducted to improve understanding of the interrelations between market chains and zoonotic infection risks at two strategic cross border points between Lao PDR, Thailand and Viet Nam. Methods: Information gained from smallholder farmer/trader interviews was integrated with serological surveys for pig-associated zoonoses-including hepatitis E virus (HEV), Taenia solium (T. solium) and trichinella-to identify potential linkages between disease risk and pig production and slaughter in low input systems common across the country. Results: Trichinella and HEV exposure was high in both humans and pigs in both study areas, significantly associated with pig slaughter and the subsequent consumption and handling of raw pork products. T. solium demonstrated a strong geographical and ethnic association with the northern study area bordering Vietnam. With the right knowledge and accessible, affordable inputs, the majority of smallholder farmers indicated a willingness to invest more in pig production, which could simultaneously improve livelihoods and decrease exposure to HEV, Trichinella, and T. solium through increased access to formal markets and an improved slaughter processes. Conclusion: The linkages identified when assessing disease risk in the context of potential economic and cultural drivers of transmission highlight the importance of a systems-based approach for the detection and control of zoonotic disease, and contributes to an improved understanding of the Lao PDR livestock sector.

• Parasites, Hosts and Diseases
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• 제53권4호
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• pp.447-453
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• 2015

The infection status of fishborne zoonotic trematode (FZT) metacercariae was investigated in fishes from 2 localities of Lao PDR. Total 157 freshwater fishes (17 species) were collected in local markets of Vientiane Municipality and Champasak Province in December 2010 and July 2011, and each fish was examined by the artificial digestion method. Total 6 species of FZT metacercariae, i.e., Opisthorchis viverrini, Haplorchis taichui, Haplorchis yokogawai, Haplorchis pumilio, Centrocestus formosanus, and Procerovum varium, were detected in fishes from Vientiane Municipality. The metacercariae of O. viverrini were detected in 50 (49.5%) out of 101 fishes (6 species), and their average number was 154 per fish infected. The remaining 5 species of heterophyid metacercariae were detected in 36.8%, 65.8%, 9.4%, 23.9%, and 5.1% fishes examined, and their average densities were 12, 1,038, 4, 15, and 13 per infected fish, respectively. In fishes from Champasak Province, 3 species of FZT metacercariae, i.e., O. viverrini, H. taichui, and H. yokogawai, were detected. Only 2 O. viverrini metacercariae were found in only 1 Barbonymus schwanefeldi. The metacercariae of H. taichui and H. yokogawai were detected in 60.0% and 50.0% of fishes examined, and their average densities were 47 and 28 per fish infected. By the present study, it has been confirmed that several species of FZT metacercariae are prevalent in fishes from Vientiane Municipality, with P. varium being a new member of FZT in Lao PDR. In comparison, FZT metacercariae are less prevalent in fishes from Champasak Province.

• Parasites, Hosts and Diseases
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• 제53권4호
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• pp.439-445
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• 2015

A survey of intestinal helminths was undertaken in riparian people in Xieng Khouang Province, Lao PDR. Fecal specimens were collected from 643 people (289 males and 354 females) residing in 4 districts (Nonghet, Kham, Phoukout, and Pek) and were examined by the Kato-Katz technique. The overall helminth egg positive rate was 41.2%, and hookworms revealed the highest prevalence (32.7%) followed by Trichuris trichiura (7.3%) and Ascaris lumbricoides (5.6%). The positive rate for small trematode eggs (STE), which may include Opisthorchis viverrini, heterophyids, and lecithodendriids, was 4.4%. For recovery of adult helminths, 12 STE or nematode/cestode egg-positive people were treated with 40 mg/kg praziquantel and 15 mg/kg pyrantel pamoate, and then purged. Mixed infections with 2 Haplorchis species (H. pumilio and H. taichui), Centrocestus formosanus, Opisthorchis viverrini, a species of cestode (Taenia saginata), and several species of nematodes including hookworms and Enterobius vermicularis were detected. The worm load for trematodes was the highest for H. pumilio with an average of 283.5 specimens per infected person followed by C. formosanus, H. taichui, and O. viverrini. The worm load for nematodes was the highest for hookworms (21.5/infected case) followed by E. vermicularis (3.2/infected case). The results revealed that the surveyed areas of Xieng Khouang Province, Lao PDR are endemic areas of various species of intestinal helminths. The STE found in the surveyed population were verified to be those of heterophyids, particularly H. pumilio.

• Parasites, Hosts and Diseases
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• 제51권3호
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• pp.305-311
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• 2013

In this study, we found that Haplorchis taichui, a heterophyid intestinal fluke, is highly prevalent, with heavy worm loads, among riparian people in Saravane and Champasak province, Lao PDR. Fecal specimens were collected from 1,460 people (717 men and 743 women) in 12 riparian (Mekong river) districts and were examined by the Kato-Katz fecal smear technique. The overall helminth egg positive rate was 78.8% and 66.4% in Saravane and Champasak province, respectively. The positive rate for small trematode eggs (STE), which included H. taichui and other heterophyids, Opisthorchis viverrini, and lecithodendriids, was 69.9% and 46.3% in Saravane and Champasak province, respectively. To obtain adult flukes, 30 STE-positive people were treated with 40 mg/kg praziquantel and then purged. Whole diarrheic stools were collected 4-5 times for each person and searched for fluke specimens using a stereomicroscope. Mixed infections with various species of trematodes (H. taichui, Haplorchis pumilio, O. viverrini, Prosthodendrium molenkampi, Centrocestus formosanus, and Echinochasmus japonicus) and a species of cestode (Taenia saginata) were found. However, the worm load was exceptionally high for H. taichui compared with other trematode species, with an average of 21,565 and 12,079 specimens per infected person in Saravane and Champasak province, respectively, followed by H. pumilio (41.9 and 22.5, respectively) and O. viverrini (9.4 and 1.5, respectively). These results show that diverse species of intestinal and liver flukes are prevalent among riparian people in Saravane and Champasak province, Lao PDR, with H. taichui being the exceptionally dominant species.

• Parasites, Hosts and Diseases
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• 제58권1호
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• pp.67-72
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• 2020

Human infection with Echinostoma aegyptica Khalil and Abaza, 1924 (Trematoda: Echinostomatidae) is extremely rare. In this study, we confirmed E. aegyptica infection in 5 riparian residents living along the Mekong River in Savannakhet Province, Lao PDR. The patients revealed eggs of Opisthorchis viverrini/minute intestinal flukes, echinostomes, and other parasites in fecal examinations using the Kato-Katz technique. Following treatment with praziquantel 30-40 mg/kg and pyrantel pamoate 10-15 mg/kg in a single dose and purging with magnesium salts, adult specimens of various helminth species were collected. Among the trematodes, echinostome flukes of 4.5-7.6 mm in length (n = 134; av. 22.3 specimens per case) were of taxonomic interest and subjected in this study. The flukes were morphologically characterized by having total 43-45 collar spines arranged in 2 alternating rows (corner spines usually 5 on each side) and compatible with previous descriptions of E. aegyptica. The patients were mixed-infected with other helminths, so specific clinical manifestations due to this echinostome fluke were difficult to determine. The present paper describes for the first time human E. aegyptica infections in Lao PDR. This is the second report of human infection (2nd-6th cases) with E. aegyptica in the world following the first one from China.

• 한국농림기상학회지
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• 제14권2호
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• pp.45-52
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• 2012

Enrichment planting은 라오스에서 산림복원 프로그램의 일환으로, 이차림에서 수종의 밀도를 증가시키는데 흔히 쓰인다. 본 연구는 다섯 종의 자생수종을 대상으로 임관층을 다양한 방법으로 소개하고 Enrichment planting을 실시하여 라오스에서 개벌 이후 적용할 수 있는 조림방법과 적합한 수종을 조사하였다. 본 연구결과, 수관층의 높이만 조사수종간 그리고 식재처리간 모두에서 통계적으로 유의한 차이가 나타났다. 묘목의 생존율과 수관층의 직경은 조사수종간에서는 유의한 차이가 나타났으나, 식재처리간에는 차이가 없었다. 반면 묘목의 근원경과 묘고는 식재처리간에서만 유의한 차이가 나타났다. 조사수종 중에는 Hopea odorata 와 Dalbergia cochinchinensis 가 다른 수종들에 비해 생존율과 수고생장 모두 양호한 것으로 나타났는데, 이는 이들 수종들이 향후 라오스 산림복원을 위한 Enrichment planting에 적용하기 적합할 것으로 판단된다. 또한 2m간격의 line planting은 묘목의 근원경 생장을 촉진시킬 수 있을 것으로 제안되지만 gap planting과 같이 넓은 간격의 line planting은 묘고생장을 촉진시킨 것으로 나타났다.

• Parasites, Hosts and Diseases
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• 제57권6호
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• pp.657-664
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• 2019

We identified the echinostome metacercariae in Chinese mystery snails, Cipangopaludina chinensis malleata, from Xiengkhuang Province, Lao PDR with morphologies of adult worms recovered. Total 20 snails were examined with artificial digestion method and then the collected metacercariae were orally infected to a mouse and a rat. Adult worms recovered from experimental animals were observed with a light microscope and a SEM. The metacercariae were round, 125×123 ㎛ in average size, with a moderately thick cyst wall, collar spines distributed in the head collar and excretory granules in 2 canals of excretory tube. Adult flukes (3-week-old in a rat) were elongated, ventrally curved and 5.310×1.023 mm in average size. Head collar distinct, bearing 43 collar spines with 5 end group ones on each side. Oral sucker subterminal, prepharynx very short, pharynx well developed, and esophagus relatively short. Cirrus sac well developed, with a saccular seminal vesicle, and ventral sucker very large. Ovary round and on the median line of the body. Testes tandom and elongated. Eggs operculated, elliptical and 90×57 ㎛ in average size. In the SEM observation, the head crown prominent, with 43 collar spines resembled with horns of younger stag. Scale-like tegumental spines were densely distributed on the surface between the head collar and ventral sucker, and their densities were decreased posteriorly. Conclusively, the metacercariae detected in C. chinensis malleata from Lao PDR were identified as those of Echinostoma macrorchis based on the morphological characteristics of adult worms.

• Parasites, Hosts and Diseases
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• 제51권2호
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• pp.259-263
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• 2013

Human taeniasis was investigated in Lao People's Democratic Republic (Lao PDR) between 2000 and 2011 as part of the nation's helminthiasis survey. A total of 55,038 inhabitants, including 29,846 school children, were examined using the Kato-Katz and scotch-tape anal swab method, and morphological observation of adult worms. Molecular identification of Taenia tapeworms was performed by multiplex PCR or DNA sequence analysis of the mitochondrial cox1 gene. Taenia eggs were present at a rate of 1.5% (845/55,038) in the subject population. Adult tapeworms were identified as T. solium or T. saginata by analyzing the collectable stool specimens (n=126). Three specimens identified as T. solium were found in Luang Prabang, while the remaining 123 specimens, which were T. saginata, were found in Bokeo, Bolikhamxay, Champasak, Houaphan, Khammouane, Luang Namta, Luang Prabang, Oudomxay, Phongsaly, Saysomboune, Saravane, Savannakhet, Xayaboury, Xekong, Xieng Khouang Province, and Vientiane Municipality.

• 적정기술학회지
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• 제7권2호
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• pp.225-234
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• 2021

이 논문의 목적은 국제개발협력의 유력한 전략으로 평가받고 있는 적정기술 사업화 모델을 라오스-한국 적정과학기술거점센터(LKSTC) 사례로 검토하는 것이다. LKSTC는 농식품 분야의 세척·수처리·살균기술, 재생에너지 분야의 피코 수력 발전기, 피코-태양광 하이브리드 시스템, 에너지 원격모니터링 기술을 개발했다. 적정기술 사업화는 카이펜 마을 기업, 학교기업, 사회적 기업을 창업했다. 정책적 시사점은 다음과 같다. 첫째, 개도국 적정기술 사업화는 수원국 정부의 지역개발 정책과 연계성을 높여야 한다. 둘째, 시장리스크를 최소화하기 위해 혁신적 기술개발 및 현지 창업네트워크를 적절히 구축해야 한다. 마지막으로 사업의 지속가능성을 높이기 위한 중장기적인 노력이 필요하다.

• Journal of Forest and Environmental Science
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• 제35권4호
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• pp.268-271
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• 2019

Mushroom survey and collection were conducted in the Phousabous National Protected Area (PNPA), which is located at the Northeastern area 230km from the capital city of Lao-PDR, Vientiane, from April 2017 to September 2018. During this periods, mushroom specimens were collected from 8 different locations, and then they were identified and classified into 284 species, 142 genera, 56 families, 20 orders and 7 classes by morphological and molecular analyses. The mushrooms belongs to Ascomycota were classified into 10 species, 5 genera, 5 families, 4 orders and 3 classes, while those belongs to Basidiomycota were classified into 274 species, 137 genera, 51 families, 16 orders, and 4 classes, respectively. Among these mushrooms, the most species-rich families are Boletaceae (16.06%), Polyporaceae (10.12%), Russulaceae (9.94%), Amanitaceae (7.68%), Agaricaceae (4.54%), Marasmiaceae (4.36%), Cortinariaceae (3.66%), Entolomataceae (3.66%), Mycenaceae (2.79%), Xylariaceae (2.44%), Physalacriaceae (2.09%), Omphalotaceae (2.09%), Hydnangitaceae (2.09%), Lyophyllaceae (1.92%), Tricholomataceae (1.75%) and comprised 75.19% of the total specimens identified.