• Journal of Life Science
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• v.16 no.7 s.80
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• pp.1104-1108
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• 2006

The purpose of this study was to investigated the effects of Laminaria japonica fucoidan extract (LJFE) through the enzymatic regulation against the hepatotoxicity-inducing carbon tetrachloride $(CCl_4)$ in LJFE and $CCl_4-treated$ rats. LJFE of 100mg/kg concentration was intraperitoneally administered into rats at dose of 1.5m11kg for 14 days. On the day 15, 3.3ml/kg of $CCl_4$ dissolved in olive oil (1:1) was injected 12 hours before anesthetization. We examined the levels of glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT) in serum of rats, superoxide dismutase(SOD) in mitochondrial fraction, and catalase(CAT), glutathione peroxidase(GPx) in liver homogenate. $CCl_4-treatment$ markedly increased the levels of GOT and GPT, and significantly decreased those of SOD, CAT and GPx. But LIFE pretreatment decreased the levels of GOT and GPT, by 40% and 64%, respectively and increased those of SOD, CAT and GPx, by 114%, 36.1% and 55.9%, respectively These results showed the LIFE had the enzymatic regulatory effects against the hepatotoxicity-inducing $CCl_4$ in the preventive way.

• Nutrition Research and Practice
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• v.12 no.3
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• pp.199-207
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• 2018

BACKGROUND/OBJECTIVES: Fermented Laminaria japonica (FL), a type sea tangle used as a functional food ingredient, has been reported to possess cognitive improving properties that may aid in the treatment of common neurodegenerative disorders, such as dementia. MATERIALS/METHODS: We examined the effects of FL on scopolamine (Sco)- and ethanol (EtOH)-induced hippocampus-dependent memory impairment, using the Passive avoidance (PA) and Morris water maze (MWM) tests. To examine the underlying mechanisms associated with neuroprotective effects, we analyzed acetylcholine (ACh) and acetylcholinesterase (AChE) activity, brain tissue expression of muscarinic acetylcholine receptor (mAChR), cAMP response element binding protein (CREB) and extracellular signal-regulated kinases 1/2 (ERK1/2), and immunohistochemical analysis, in the hippocampus of mice, compared to current drug therapy intervention. Biochemical blood analysis was carried out to determine the effects of FL on alanine transaminase (ALT), aspartate transaminase (AST), and triglyceride (TG) and total cholesterol (TC) levels. 7 groups (n = 10) consisted of a control (CON), 3 Sco-induced dementia and 3 EtOH-induced dementia groups, with both dementia group types containing an untreated group (Sco and EtOH); a positive control, orally administered donepezil (Dpz) (4mg/kg) (Sco + Dpz and EtOH + Dpz); and an FL (50 mg/kg) treatment group (Sco + FL50 and EtOH + FL50), orally administered over the 4-week experimental period. RESULTS: FL50 significantly reduced EtOH-induced increase in AST and ALT levels. FL50 treatment reduced EtOH-impaired step-through latency time in the PA test, and Sco- and EtOH-induced dementia escape latency times in the MWM test. Moreover, anticholinergic effects of Sco and EtOH on the brain were reversed by FL50, through the attenuation of AChE activity and elevation of ACh concentration. FL50 elevated ERK1/2 protein expression and increased p-CREB (ser133) in hippocampus brain tissue, according to Western blot and immunohistochemistry analysis, respectively. CONCLUSION: Overall, these results suggest that FL may be considered an efficacious intervention for Sco- and EtOH-induced dementia, in terms of reversing cognitive impairment and neuroplastic dysfunction.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.4
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• pp.438-443
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• 1999

Germination and growth of Laminaria japonica microscopic stages were investigated under crossed gradients of temperatures and irradiances, and the results related to the seasonal temperature regime in the southeastern coast of Korea. Germination rates of $70\~86\%$ were observed in the temperature range of $5\~20^{\circ}C$, however, at $25^{\circ}C$ no germination of meiospores was observed. The primary factor affecting germination rates at the temperature range of $5\~20^{\circ}C$ was irradiance: germination was significantly reduced at $150 {\mu}E{\cdot}m^{-2}{\cdot}s^{-1}$. Vegetative cell production of female gametophytes was highest at $20^{\circ}C$, but plants were not fertile at the temperature. In the temperature range of $5\~15^{\circ}C$, higher irradiance caused females to reduce cell production, but increased fertility. Cell production was also low at lower temperatures with increased fertility rates. Optimal growth temperature for microsporophytes was $10^{\circ}C$ and their growth rates were light-saturated at $70 {\mu}E{\cdot}m^{-2}{\cdot}s^{-1}$. We have concluded that meiospores released before July could develope to the young sporophytic stage in the southeastern coast of Korea which is off the southern limit of its geographical distribution. However, limiting factor in the development of natural sporophytic population in this region would be the upper temperature limit for the survival of young sporophytes, as water temperature at this area frequently exceeds $25^{\circ}C$ during the summer period.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2006.05a
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• pp.333-337
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• 2006

Biosorption can be used to eliminate heavy metals from industrial effluents or to recover precious metals from processing solutions. Scanning Electron Microscopedetected a high Cr sorption capacity of L. Japonica biomass almost 45% of its dry weight at pH 4.5. It should be mentioned that adjusting the pH influences on sorption capacity of biomass.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.126-127
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• 2000

후코이단은 혈액중에 존재하는 함황 산성다당인 헤파린(heparin)과 구조 및 생리적 특성이 유사하여 항혈액응고작용(Bernardi and Springer, 1962)을 갖고 있다는 사실이 처음으로 밝혀지게 되었다. 그후 Usui 등(1980)은 Eisenia bicyclis로부터 추출한 후코이단 획분의 항혈액응고 활성이 54 I.U.로서 헤파린의 40%정도의 높은 활성을 갖고 있다는 사실이 증명되었다. (중략)

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.128-129
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• 2000

지금까지 해양생물에 관한 연구로서 갈조류중의 미역(Undaria pinnatifida)의 주성분인 알긴산(alginic acid)의 생리활성을 중심으로 성인병의 예방과 노화 억제효과에 관한 연구가 진행되어 왔다. 미역, 다시마 등 갈조류의 생리활성성분으로서 알긴산은 점성(viscosity)이 매우 커서 음료개발에 많은 문제점을 갖고 있다. 그렇지만, 후코이단 성분은 중성다당인 라미나란(laminaran)과는 달리 황산기(sulfate group)를 가진 산성의 수용성 다당류로서 항혈액응고 작용과 항종양 활성을 나타낸다고 보고되어졌다. (중략)

• Proceedings of the Korean Society of Life Science Conference
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• 2001.09a
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• pp.41-41
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• 2001

• Proceedings of the Korean Society of Life Science Conference
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• 2001.09a
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• pp.43-43
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• 2001

• Proceedings of the Korean Society of Life Science Conference
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• 2001.09a
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• pp.44-44
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• 2001

• Proceedings of the Korean Society of Life Science Conference
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• 2001.09a
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• pp.42-42
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• 2001