• Title/Summary/Keyword: Lambda

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Construction of a cDNA library of Aphis gossypii Glover for use in RNAi

  • KWON, HyeRi;KIM, JungGyu;LIM, HyounSub;YU, YongMan;YOUN, YoungNam
    • Entomological Research
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    • v.48 no.5
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    • pp.384-389
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    • 2018
  • Aphis gossypii Glover is an important insect pest that functions as a viral vector and mediates approximately 45 different viral diseases. As part of a strategy for control of A. gossypii, we investigated the functions of genes using RNAi. To this end, a cDNA library was constructed for various genes and for selecting appropriate targets for RNAi mediated silencing. The cDNA library was constructed using the Gateway cloning system with site-specific recombination of bacteriophage ${\lambda}$. It was used to carry out single step cloning of A. gossypii cDNAs. As a result, a cDNA library with a titer of $8.4{\times}10^6$ was constructed. Since the sequences in this library carry att sites, they can be cloned into various binary vectors. This library will be of value for various studies. For later screening of selected genes, it is planned to clone the library into virus-induced gene silencing (VIGS) vectors, which makes it possible to analyze gene function and allow subsequent transfection of plants. Such transfection experiments will allow testing of RNAi-induced insecticidal activity or repellent activity to A. gossypii, and result in the identification of target genes. It is also expected that the constructed cDNA library will be useful for analysis of gene functions in A. gossypii.

Visualization of Self-Healing Function of Protective Coating for Concrete (콘크리트 보호코팅재의 자기치유 기능의 시각화)

  • Kim, Dong-Min;Choi, Ju-Young;Jin, Seung-Won;Nam, Kyeong-Nam;Park, Hyeong-Joo;Chung, Chan-Moon
    • Journal of Convergence for Information Technology
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    • v.9 no.10
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    • pp.87-93
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    • 2019
  • Microcapsules were prepared by using a mixture of linseed oil and a small amount of fluorescent fluid as a core material. Self-healing protective coatings were prepared by applying coating formulations containing varying amounts of microcapsules on mortar surface. After scratch or crack was generated in the coating, when the damaged region was exposed to ultraviolet light (${\lambda}=365nm$), it was observed that fluorescence emission area increased with increasing microcapsule loading. In the cases of the self-healing coatings having 20wt% or more microcapsule loading, the damaged region was almost filled with the healing agent. In water sorptivity test, the self-healing coating having 20wt% or more microcapsule loading showed a healing efficiency of about 85%. The fluorescence emission from the damaged region was easily observed at a distance of 3 m. The self-healing protective coating is expected to be useful to confirm its self-healing function with the eye.

Direct Synthesis and Structure of $\eta^4$-1-Functionally Substituted 2,3,4,5-Tetraphenyl-1-Silacyclopenta-2,4-diene Complexes of Irontricarbonyl

  • Joo, Qan-Chul;Sohn, Hong-Lae;Hong, Jang-Hwan;Kong, Young-Kun;Singh, P.
    • Bulletin of the Korean Chemical Society
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    • v.10 no.2
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    • pp.191-196
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    • 1989
  • We obtained the new complexes, $Fe{\eta}^4-R,R'-TPSCp](CO)_3$(R,R'-TPSCp = 1,1-disubstituted 2,3,4,5-Tetraphenyl-1-Silacyclopenta-2,4-diene; R = Ph, R' = Cl, R = R' = Cl) from the reaction of the corresponding R,R'-TPSCp with ironpentacarbonyl under reflux in toluene. Also, the analogous complexes with R = R' = Me and R = Me, R' = Cl were obtained in an identical manner. We have determined the crystal structure of $Fe[Ph(Cl)-TPSCp](CO_)3$ by using Mo ka, ${\lambda}$ = 0.71069${\AA}$, where the unit cell was found to be monoclinic with a = 9.042 (6)${\AA}$, b = 19.870 (9)${\AA}$, c = 17.426 (9)${\AA}$ and ${\beta}$ = 96.28(4)$^{\circ}$. The butadiene moiety of TPSCp ring is planar and the dihedral angle of the butadiene plane and C4-Si-C25 plane was opened up to 41.8$^{\circ}$. The C-C distances in the butadiene moiety were found to be 1.4346, 1.462, and 1.440 ${\AA}$, respectively. It may be said that the four ${\pi}$-electrons are delocalized over the four carbons in five membered ring through coordination with ironcarbonyl. In this complex Fe is either in distorted tetrahedron environment with the centroid of the four C-atom butadiene moiety and three carbons of the three carbonyls or in distorted square-pyramidal environment with two midpoints of double bonds of the butadiene moiety and two carbons of carbonyl defining the base of the pyramid and the carbon of remaining carbonyl the apex.

Synthesis of Alkylidyne Complexes of Br$(CO)_2(tmeda)M{\equiv}CC_{6}H_{4}Me$ (M = Cr, Mo, W). Crystal Structure of Br$(CO)_2(tmeda)M{\equiv}CC_{6}H_{4}Me$

  • Park, Joon T.;Cho, Jeong-Ju;Suh, Il-Hwan;Lee, Jin-Ho;Lim, Sung-Su;Ryu, Bo-Young
    • Bulletin of the Korean Chemical Society
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    • v.14 no.2
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    • pp.266-271
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    • 1993
  • The reaction of $(CO_5$)M=C(OMe)Tol (M=Cr, Mo, W and $Tol=p-C_6H_4Me)$ and $BBr_3$ followed by treatment with tetramethylethylenediamine (TMEDA) yields a mixture of two diastereomers, trans, $cis-Br(CO)_2(tmeda)M{\equiv}$CTol [M=Cr(1a), Mo(2a), W(3a)] and cis, $trans-Br(CO)_2(tmeda)M{\equiv}$CTol [M=Cr(1b), Mo(2b), W(3b)], respectively. These compounds have been isolated as crystalline solids and characterized by spectroscopic (infrared, mass, $^1H$ and $^{13}C-NMR)$ data. The trans, cis-Br(CO)2(tmeda)Cr${\equiv}$CTol (1a), has been examine via a single crystal X-ray diffraction study : $BrCrO_2N_2C_{16}H_{23}$, Mr=407.27, triclinic, $P{\bar{1}},\;a=12.792(2),\;b=13.400(5),\;c= 11.645(4)\;{\AA},\;{\alpha}=101.26(2)^{\circ},\;{\beta}=103.04(2)^{\circ},\;{\gamma}=91.88(2)^{\circ},\;{\nu}=1907(1){\AA}^3,\;Z=2,\;{\rho}(calcd)=1.418\;gcm^{-3},\;{\lambda}(MoK{\alpha})=0.71069\;{\AA},\;{\mu}=26.25 cm^{-1},\;F(000)=831.97,\;T=295K,\;R=0.0977$ for 1332 significant reflections $[F_0>5{\sigma}(F_0)]$. There are two essentially equivalent molecules in the crystallographic asymmetric unit. Each molecule is octahedral with the bromide ligand trans to the alkylidyne carbon, the two cis-carbonyl ligands, and the bidentate TMEDA ligand.

Improvement of Catastrophic Forgetting using variable Lambda value in EWC (가변 람다값을 이용한 EWC에서의 치명적 망각현상 개선)

  • Park, Seong-Hyeon;Kang, Seok-Hoon
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.25 no.1
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    • pp.27-35
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    • 2021
  • This paper proposes a method to mitigate the Catastrophic Forgetting phenomenon in which artificial neural networks forget information on previous data. This method adjusts the Regularization strength by measuring the relationship between previous data and present data. MNIST and EMNIST data were used for performance evaluation and experimented in three scenarios. The experiment results showed a 0.1~3% improvement in the accuracy of the previous task for the same domain data and a 10~13% improvement in the accuracy of the previous task for different domain data. When continuously learning data with various domains, the accuracy of all previous tasks achieved more than 50% and the average accuracy improved by about 7%. This result shows that neural network learning can be properly performed in a CL environment in which data of different domains are successively entered by the method of this paper.

Adaptive Weight Control for Improvement of Catastropic Forgetting in LwF (LwF에서 망각현상 개선을 위한 적응적 가중치 제어 방법)

  • Park, Seong-Hyeon;Kang, Seok-Hoon
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.26 no.1
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    • pp.15-23
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    • 2022
  • Among the learning methods for Continuous Learning environments, "Learning without Forgetting" has fixed regularization strengths, which can lead to poor performance in environments where various data are received. We suggest a way to set weights variable by identifying the features of the data we want to learn. We applied weights adaptively using correlation and complexity. Scenarios with various data are used for evaluation and experiments showed accuracy increases by up to 5% in the new task and up to 11% in the previous task. In addition, it was found that the adaptive weight value obtained by the algorithm proposed in this paper, approached the optimal weight value calculated manually by repeated experiments for each experimental scenario. The correlation coefficient value is 0.739, and overall average task accuracy increased. It can be seen that the method of this paper sets an appropriate lambda value every time a new task is learned, and derives the optimal result value in various scenarios.

Dislocation in Semi-infinite Half Plane Subject to Adhesive Complete Contact with Square Wedge: Part II - Approximation and Application of Corrective Functions (직각 쐐기와 응착접촉 하는 반무한 평판 내 전위: 제2부 - 보정 함수의 근사 및 응용)

  • Kim, Hyung-Kyu
    • Tribology and Lubricants
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    • v.38 no.3
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    • pp.84-92
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    • 2022
  • In Part I, developed was a method to obtain the stress field due to an edge dislocation that locates in an elastic half plane beneath the contact edge of an elastically similar square wedge. Essential result was the corrective functions which incorporate a traction free condition of the free surfaces. In the sequel to Part I, features of the corrective functions, Fkij,(k = x, y;i,j = x,y) are investigated in this Part II at first. It is found that Fxxx(ŷ) = Fxyx(ŷ) where ŷ = y/η and η being the location of an edge dislocation on the y axis. When compared with the corrective functions derived for the case of an edge dislocation at x = ξ, analogy is found when the indices of y and x are exchanged with each other as can be readily expected. The corrective functions are curve fitted by using the scatter data generated using a numerical technique. The algebraic form for the curve fitting is designed as Fkij(ŷ) = $\frac{1}{\hat{y}^{1-{\lambda}}I+yp}$$\sum_{q=0}^{m}{\left}$$\left[A_q\left(\frac{\hat{y}}{1+\hat{y}} \right)^q \right]$ where λI=0.5445, the eigenvalue of the adhesive complete contact problem introduced in Part I. To investigate the exponent of Fkij, i.e.(1 - λI) and p, Log|Fkij|(ŷ)-Log|(ŷ)| is plotted and investigated. All the coefficients and powers in the algebraic form of the corrective functions are obtained using Mathematica. Method of analyzing a surface perpendicular crack emanated from the complete contact edge is explained as an application of the curve-fitted corrective functions.

A Comparison of Performance Evaluations of Reusable Masks (다회용 마스크 유형에 따른 성능 비교연구)

  • Ryu, Hoe-Jin;Park, Won-Hee;Lee, Chun-Yeong;Jeong, Seon-Ok;Choi, Hee-Jin;Lee, Jeong-Mi;Hwang, In-Sook;Shin, Yong-Seung
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.32 no.1
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    • pp.53-63
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    • 2022
  • Objectives: This study was designed to evaluate the filtration efficiency, inhalation resistance, and liquid resistance of commercial reusable masks in comparison to the performance of KF-AD masks (reference masks). Methods: Thirty-six masks were purchased from an online market. Filtration efficiency and inhalation resistance were determined in the same manner as in MFDS certification testing, respectively using TIL/IL & Filter Test 1300S (SFP Services, UK) and Breathing Resistance Analyzer DA2001 (Lambda Ray, South Korea). Results: Most of the filtration efficiencies found for the 19 masks were far lower than the test standard for KF-certified masks. Four masks met KF94 and three masks even met KF80. Most inhalation resistances were also much lower than the standard, with many almost one-half of the standard. In addition, all the masks except KF-AD masks did not pass the liquid resistance test. Conclusions: Although most of the filtration efficiency performance results found in this study for the reusable masks, which emerged as an alternative, were lower than the standard for health masks, multi-use masks can be used with a replacement filter inserted.

Prediction of rainfall-induced runoff considering infiltration of water in both unsaturated and saturated porous media (불포화 및 포화 투수층에서의 침투를 고려하여 강우 유출 해석)

  • Changhoon Lee;Minh Thang Tran
    • Proceedings of the Korea Water Resources Association Conference
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    • 2023.05a
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    • pp.62-62
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    • 2023
  • 강우가 지표면 아래로 침투할 때 초기에는 투수층이 불포화 상태이어서 부압이 작용하면서 침투할 것이다. Richards 식(Richards, 1931)을 써서 불포화 투수층의 침투를 모의할 수 있다. 강우가 지속되는 동안 하상 아래 어느 구간은 포화 상태가 되어 Richards 식을 더 이상 사용할 수 없다. 하지만 현재까지의 연구는 Richards 식을 사용하여 침투를 모의하는 오류를 범하고 있다. 강우에 의한 침투를 예측할 때 지표면에서의 침투율 qb 가 필요한 데 현존하는 연구에서는 Horton 식(Horton, 1941)을 사용하여 초기 침투율 fo 와 장시간 후 침투율 fc 와 시간에 따라 지수함수로 감소하는 계수 k 의 3가지 계수값을 실험이나 현장 관측값에서 찾아서 쓰고 있다. 그런데, 이 계수값은 강우강도 ri 가 클수록 침투율 q 가 커지는 물리 현상을 반영하지 못하는 한계가 있다. 본 연구에서 먼저 포화 투수층에서의 침투를 모의하는 식을 개발하였다. 지표면 아래에서 불포화 투수층에는 Richards식을 사용하고 포화 투수층에는 개발한 식을 사용하여 침투를 모의하였다. 또한 지표면에서의 침투율 qb 를 구하는 공식을 개발하였다. 하상에서의 침투율의 최대값은 $q_{bmax}=-{\lambda}{\sqrt{2g(s-b)}}$ 일 것이다. 여기서 λ 는 투수층의 공극율, s 는 유출수면의 위치, b 는 지표면의 위치이다. 지표면에서의 침투율의 최소값 qbmin 은 지표면 바로 아래 지점에서의 침투율일 것이다. 지표면에서의 침투율 qb 로 qbmax 와 qbmin 사이의 적절한 값을 선택한다. 강우강도를 ri 라고 하면 지표면 위 유출수의 연속방정식은 다음과 같다: $s-b={\int}(r_i-{\mid}q_b{\mid})dt$. 즉, 유출수면의 위치 s 는 강우강도 ri 가 클수록 또는 지표면에서의 유출율의 크기 |qb| 가 작을수록 크다. 또한 지표면에서의 침투율 qb 와 지표면 아래에서의 침투율 q 는 s - b 가 클수록 크다. 따라서, 강우강도 ri 가 클수록 침투율 qb, q 가 큰 현상이 잘 반영되었다. 강우-침투-유출 모형실험을 수행하여 강우강도에 따라 침투율과 유출량이 다른 현상을 관측하여 수치실험 결과와 비교·검증하였다.

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Machine Learning Model for Recommending Products and Estimating Sales Prices of Reverse Direct Purchase (역직구 상품 추천 및 판매가 추정을 위한 머신러닝 모델)

  • Kyu Ik Kim;Berdibayev Yergali;Soo Hyung Kim;Jin Suk Kim
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.46 no.2
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    • pp.176-182
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    • 2023
  • With about 80% of the global economy expected to shift to the global market by 2030, exports of reverse direct purchase products, in which foreign consumers purchase products from online shopping malls in Korea, are growing 55% annually. As of 2021, sales of reverse direct purchases in South Korea increased 50.6% from the previous year, surpassing 40 million. In order for domestic SMEs(Small and medium sized enterprises) to enter overseas markets, it is important to come up with export strategies based on various market analysis information, but for domestic small and medium-sized sellers, entry barriers are high, such as lack of information on overseas markets and difficulty in selecting local preferred products and determining competitive sales prices. This study develops an AI-based product recommendation and sales price estimation model to collect and analyze global shopping malls and product trends to provide marketing information that presents promising and appropriate product sales prices to small and medium-sized sellers who have difficulty collecting global market information. The product recommendation model is based on the LTR (Learning To Rank) methodology. As a result of comparing performance with nDCG, the Pair-wise-based XGBoost-LambdaMART Model was measured to be excellent. The sales price estimation model uses a regression algorithm. According to the R-Squared value, the Light Gradient Boosting Machine performs best in this model.