• 한국환경과학회:학술대회논문집
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• 한국환경과학회 2003년도 International Symposium on Clean Environment
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• pp.99-102
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• 2003

The different methods such as HPLC, indirect- and direct-ELISA were employed for the analysis of microtoxins and the results of each method were compared in terms of the detection limit and accurary. Three toxins, microcystin-RR, -LR and -YR were clearly separated by HPLC using 0.05 M methanol and phosphate buffer used as a solvent system. The calibration curves for the toxins were linear in the range of 5 ng to 50 ng. The standard curves for the immunoassay of microcystin obtained by direct and indirect ELISA are compared. The linear responses of inhibitions of binding by microcystin in the direct and indirect competitive ELISA were in the range of 10 ng to 1000 ng and 50 pg to 160 pg, respectively. Distribution of microtoxins at 11 sites in the Nakdong river and several lakes in Korea was also studied. The most dominant microcystin variant in the test sites was found to be microcystin-RR.

• 한국환경과학회지
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• 제4권5호
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• pp.461-468
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• 1995

In order to provide for the guidance on groundwater quality monitoring network design and also, to suggest the index to the solution of the contaminated groundwater remediation problems in the lake watershed, it is necessary to analyze the contaminant transport in the groundwater. The solute transport was analyzed in the lake watershed to investigate the behavior of the injected contaminant sources depend on the relationships between the point of contaminant sources and position of the lake. Three hypothetical groundwater flow systems, which is composed of a flow-through lake and two solute sources, were considered. The lakes located in the upper, middle, and lower portions of a watershed respectively. The transported contaminant was numerically simulated for five years by using MT3D contaminant transport model under the three-dimentional steady state conditions. From the above simulations, it can be concluded that the contaminant concentration was high as the contaminant source located at the upper position of a watershed, and the influence of the contaminant injection was large as the solute source located at the lower position. When the injection of contaminant was continued for one year without regard to the position of contaminant source and the lake, the influence of contaminant source was reached to bedrock.

• 한국환경과학회지
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• 제12권2호
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• pp.163-170
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• 2003

Seosan A, B area is located on 36°42' of north parallel and 126°27' of east longitude. It consisted of broad artificial lakes and reclaimed agricultural lands; there is Ganwalho lake in A area and Bunamho lake in B area. Total birds surveyed four times in 2001 at the A area are 105,580 of 11 orders, 28 families, 89 species. Among them, resident species were 21 species including Pica pica. Twenty species including Cuculus canorus were summer migrants, and 30 species including Platalea leucorodia were winter migrants. Seventeen species including Limosa limosa were occasionally species. And Rostratula benghalensis was an unconfirmed species. Species diversity index (H') was 0.72; species equally common index (e/sup H'/) was 2.06. In January, maximum observed 102,121 individuals. However in July 52 species were observed most variously. There were total 81,152 birds observed with 11 orders, 22 families, and 71 species at the B area. Fifteen species including Falco tinnumculus were resident species. Summer migrants were 18 species including Ixobychus sinensis. Winter migrants were 25 species including Ciconia boyciana. Thirteen species including Tringa glareola were occasionally species. Species diversity index (H') was 0.281; species equally common index (e/sup H'/) was 1.325. In January, maximum observed 78,433 individuals. However in Silly 42 species were observed most variously.

• 한국환경과학회지
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• 제11권12호
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• pp.1281-1289
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• 2002

This study was conducted to evaluate water quality in the Keum River using multivariate analysis. The analysis data in Keum river made use of surveyed data by the ministry of environment from January 1994 to December 2001. Thirteen water quality parameter were determined on each sample. The results was summarized as follow; Water quality in the Keum River could be explained up to 71.39% by four factors which were included in loading of organic matter and nutrients by the tributaries (32.88%), seasonal variation (16.09%), loading of pathogenic bacteria by domestic sewage of Gapcheon (13.39%) and internal metabolism in estuary as lakes(9.03%). For spatial variation of factor score, four group was classified by each factor characterization. Station 1 and 2 was influenced by Daechung dam, station 3 was affected by domestic sewage of Gapcheon, station 10~12 was affected by estuary dyke and the rest station. The result of cluster analysis by station was classified into four group that has different water quality characteristics. In monthly cluster analysis, three group was classified according to seasonal characteristic. Also, in yearly cluster analysis, three group was classified. It is necessary to control the pollutant loadings by Gapcheon inflow domestic sewage in Daejeon city for the sake of water quality management of Keum river.

• 대한기계학회논문집B
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• 제28권5호
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• pp.604-611
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• 2004

Simulation of three-dimensional turbulent flow with LES and DNS lakes much time and expense with currently available computing resources and requires big computing resources especially for high Reynolds number. The emerging alternative to provide the required computing power and working environment is the Grid computing technology. We developed the CFD code which carries out the parallel computing under the Grid environment. We constructed the Grid environment by connecting different PC-cluster systems located at two different institutes of Pusan National University in Busan and KISTI in Daejeon. The specification of PC-cluster located at two different institutes is not uniform. We run our parallelized computer code under the Grid environment and compared its performance with that obtained using the homogeneous computing environment. When we run our code under the Grid environment, the communication time between different computer nodes takes much larger time than the real computation time. Thus the Grid computing requires the highly fast network speed.

• BMB Reports
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• 제39권6호
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• pp.782-787
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• 2006

A new protein was cloned and identified as the sixth subunit of Choristoneura fumiferana origin recognition complex (CfORC6). The newly identified 43 kDa protein CfORC6 is much bigger than DmORC6 (25.7 kDa) and HsORC6 (28.1 kDa), though it's 23.85% identical to DmORC6 and 23.81% identical to HsORC6. Although the molecular weight of CfORC6 is close to ScORc6 (50 kDa), CfORC6 is only 14.03% identical to ScORC6. By alignment, it was found that the N-terminal of CfORC6 has about 30% identities with other ORC6s, but about 100aa of C-terminal of CfORC6 has no identity with other ORC6s. Like ScORC6, CfORC6 has many potential phosphorylation sites, (S/T)PXK. Like DmORC6, CfORC6 has leucine-rich region in the relevant site. Northern Blot showed that CfORC6 mRNA is about 2,000nt. Southern Blot confirmed that there is one copy of CfORC6 gene in spruce budworm genome. Western blot showed that infection of Cf124T cells with CfMNPV didn't affect the expression levels of CfORC6, at least up to 26 hr post infection.

• Journal of Microbiology
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• 제43권2호
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• pp.144-151
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• 2005

During the past ten years, Archaea have been recognized as a widespread and significant component of marine picoplankton assemblages. More recently, the presence of novel archaeal phylogenetic lineages has been discovered in coastal marine environments, freshwater lakes, polar seas, and deep-sea hydrothermal vents. Therefore, we conducted an investigation into the archaeal community existing in tidal flat sediment collected from Ganghwa Island, Korea. Phylogenetic analysis of archaeal 16S rDNA amplified directly from tidal flat sediment DNA revealed the presence of two major lineages, belonging to the Crenarchaeota ($53.9\%$) and Euryarchaeota ($46.1\%$) phyla. A total of 102 clones were then sequenced and analyzed by comprehensive phylogenetic analysis. The sequences determined in our samples were found to be closely related to the sequences of clones which had been previously obtained from a variety of marine environments. Archaeal clones exhibited higher similarities ($83.25 - 100\%$) to sequences..from other environments in the public database than did those ($75.22 - 98.46\%$) of previously reported bacterial clones obtained from tidal flat sediment. The results of our study suggest that the archaeal community in tidal flat sediment is remarkably diverse.

• 대한공간정보학회지
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• 제13권1호
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• pp.71-80
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• 2005

사회 환경의 급격한 변화에 따라 하천과 호소의 수질이 극도로 오염되면서 그에 따른 수질문제가 해결해야 할 중대한 사안으로 부각되고 있다. 저수지 오염불질의 저감을 위해서는 정확한 저수질 예측이 필요하며 그에 따른 오염물질 저감대책이 수립되어야 한다. 본 연구의 목적은 전문가시스템과 GIS를 도입하여 저수지 수질정보의 관리, 수질 예측과 수질개선 대책 수립을 위한 의사결정을 지원하는 프로토타입의 지능형 지리정보시스템을 개발하는데 있다. 주연구내용은 시스템 분석 및 설계, 지리정보데이터 수집과 데이터베이스 구축, 저수지 수질정보 수집 및 분석, 저수지 수질예측을 위한 GIS, WASPS 및 전문가시스템의 통합 인터페이스 구축이다.

• Fisheries and Aquatic Sciences
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• 제13권3호
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• pp.244-253
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• 2010

The quality of marine sediments from the industrialized coastal areas of Korea (Ulsan Bay, Masan Bay, and artificial Lake Shihwa) was investigated using a bacterial bioluminescence toxicity test. Sediment toxicity results were compared with the levels of chemical contamination (trace metals, organic wastewater markers, acid volatile sulfides, total organic carbon). Effective concentration 50% (EC50) of sediments ranged from 0.014 to 1.126 mg/mL, which is comparable to or lower than values in contaminated lakes, rivers, and marine sediments of other countries. Sediment reference index (SRI) ranged from 13 to 1044, based on the EC50 of the negative control sample. Mean average SRI values in Masan Bay and Lake Shihwa were approximately 8 and 9 times as high as that in Ulsan Bay, indicating higher sediment toxicity and greater contamination in the two former regions. Sediment toxicity were strongly associated with the concentrations of some chemicals, suggesting that this test may be useful for determining potential chemical contamination in sediments.

• 한국물환경학회지
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• 제29권5호
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• pp.691-702
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• 2013

This review paper summarizes the theory, application, and potential drawbacks of diffusive gradient in thin film (DGT) probe which is a widely used in-situ passive sampling technique for monitoring inorganic contaminants in aquatic environments. The DGT probe employs a series of layers including a filter membrane, a diffusive hydrogel, and an ionic exchange resin gel in a plastic unit. The filter side is exposed to an aquatic environment after which dissolved inorganic contaminants, such as heavy metals and nuclides, diffuse through the hydrogel and are accumulated in the resin gel. After retrieval, the contaminants in the resin gel are extracted by strong acid or base and the concentrations are determined by analytical instruments. Then aqueous concentrations of the inorganic contaminants can be estimated from a mathematical equation. The DGT has also been used to monitor nutrients, such as ${PO_4}^{3-}$, in lakes, streams, and estuaries, which might be helpful in assessing eutrophic potential in aquatic environments. DGT is a robust in-situ passive sampling techniques for investigating bioavailability, toxicity, and speciation of inorganic contaminants in aquatic environments, and can be an effective monitoring tool for risk assessment.