• Journal of Food Hygiene and Safety
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• v.13 no.2
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• pp.83-86
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• 1998

This study was carried out to measure the antibacterial effect of lactoperoxidase system against L. monocytogenes. When the initial inoculum levels ($10^{2},\;10^{4},\;10^{7}\;CFU/ml$), concentration of LP (10 ppm, 20 ppm, 30 ppm), culture media (TSB-YE, UHT milk) and storage temperatures ($5^{\circ}C,\;10^{\circ}C,\;15^{\circ}C$) were set up differently for the experiment and the antibacterial effect was compared, the highest antibacterial effect of LP system was shown at $10^{2}\;cfu/ml$ of initial inoculum level, 10 ppm of LP concentration and $5^{\circ}C$ of incubaction temperature. The antibacterial effect of LPS in UHT milk was similar to that in Tryptic soy broth.

• Journal of Food Hygiene and Safety
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• v.13 no.1
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• pp.1-5
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• 1998

This study was carried out to measure the antibacterial effect of lactoperoxidase/ thiocyanate/hydrogen peroxide system (LP system) against E. coli 0157:H7. When the initial inoculum levels (($10^{2},\;10^{4},\;10^{7}cfu/ml$), concentration of LP (10 ppm, 20 ppm, 30 ppm), culture media (TSB-YE, UHT milk, raw milk) and storage temperatures ($5^{\circ}C,\;10^{\circ}C,$15$^{\circ}C$) were set up differently for the experiment and the antibacterial effect was compared, the highest antibacterial effect of LP system was shown at $10^{2}cfu/ml$ of initial inoculum level, 10 ppm of LP concentration and $5^{\circ}C$ of incubation temperature.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.1
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• pp.109-112
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• 2006

The objective of this study was to evaluate the combined effects of lactoperoxidase system (LPS), thermal treatment and storage time on total microflora and psychrotrophs counts of the marinated broiler drumsticks. A marinade that contained acetic acid (1%) and salt (3%) with pH adjusted to 4 was developed as a standardized marinade. Drumsticks were marinated with various LPS levels, combined with thermal treatment (4 or $58^{\circ}C$ for 2 min), and then stored at $4^{\circ}C$ for 18 h. The microbial counts of the samples were measured after 0, 2, 4 and 7 days of storage for drumsticks held at $4^{\circ}C$. The results indicate that adding LPS at the level of 1 unit ($1{\mu}g/ml$ LP, 5.9 mM KSCN, and 2.5 mM $H_2O_2$) significantly (p<0.05) decreased the total microflora and psychrotrophs counts of the marinated broiler drumsticks. In addition, samples treated with a thermal treatment ($58^{\circ}C$ for 2 min) had significantly (p<0.05) lower microbial counts when compared with the control.

• Journal of Dairy Science and Biotechnology
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• v.2 no.1
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• pp.29-33
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• 1982

• Korean Journal of Food Science and Technology
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• v.28 no.4
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• pp.736-742
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• 1996

This study was carried out to investigate the effect of the lactoperoxidase (LP) system on the acid production of gel type yogurt. The LP system was activated by adding freshly prepared solutions of 1 ppm (v/w) lactoperoxidase and three different concentrations (0.125 mM, 0.25 mM, 0.5 mM) in equimolar ratios of KSCN and $(H_{2}O_{2}$, In 0.25 mM treated samples for the 4 hr fermentation resulted in titratable acidity of $0.4{\%}$, pH of 5.06, lactic acid bacterial count of $3.0{\times}10^{8}\;CFU/ml and acetaldehyde concentration of 10.2 ppm, whereas the untreated samples were 1.0%, 4.54, $4.7{\times}10^{9}\;CFU/ml and 18.0 ppm, respectively. The residual amount of KSCN in 0.25 mM treated samples was determined during the experiments, which decreased to 4.4 ppm. There was no detectable $(H_{2}O_{2}$ for 6 hr fermentation. However, residual KSCN and $(H_{2}O_{2}$ concentrations in 0.5mM treated samples were 5.7 and 8.4 ppm, respectively. These results have indicated that the optimum concentration of $(H_{2}O_{2}$ and KSCN to activate the LP system was 0.25 mM each.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 1997.11a
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• pp.126-126
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• 1997

• Journal of Oral Medicine and Pain
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• v.33 no.1
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• pp.1-8
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• 2008

It is well known that many antimicrobial proteins in saliva interact with each other. The purpose of the present study was to investigate the interactions of lysozyme with peroxidase in the aspects of enzymatic activity in vitro. The interactions of lysozyme with peroxidase were examined by incubating hen egg-white lysozyme(HEWL) with bovine lactoperoxidase(bLP). The influence of peroxidase system on lysozyme was examined by subsequent addition of potassium thiocyanate and hydrogen peroxide. Lysozyme activity was determined by turbidity measurement of a Micrococcus lysodeikticus substrate suspension. Peroxidase activity was determined with an NbsSCN assay. The Wilcoxon signed rank test was used to analyze the changes of enzymatic activities compared with their controls. bLP at physiological concentrations enhanced the enzymatic activity of HEWL(P < 0.05) and its effect was dependent on the concentration of peroxidase. However, HEWL did not affect the enzymatic activity of bLP. Thiocyanate did not affect the enzymatic activity of HEWL, either. The addition of potassium thiocyanate and hydrogen peroxide did not lead to additional enhancement of the enzymatic activity of HEWL. The changes of hydrogen peroxide concentration in the peroxidase system did not affect the enzymatic activity of HEWL. Collectively, despite an in vitro nature of our study, the results of the present study provide valuable information on the interactions of lysozyme and peroxidase in the aspects of enzymatic activity in oral health care products and possibly in the oral cavity.

• Journal of Oral Medicine and Pain
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• v.43 no.1
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• pp.1-7
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• 2018

Purpose: The purpose of the study was to investigate the influences of hyaluronic acid on the candidacidal activities of lysozyme, the peroxidase system, and the glucose oxidase-mediated peroxidase (GO-PO) system at different concentrations of antimicrobial enzymes. Methods: Hyaluronic acid was used at a final concentration of 0.5 mg/mL. Hen egg-white lysozyme (HEWL) was used at concentrations ranging from 10 to $100{\mu}g/mL$. The peroxidase system included bovine lactoperoxidase (bLPO), potassium thiocyanate (KSCN, 1 mM), and hydrogen peroxide ($100{\mu}M$). The GO-PO system included bLPO, KSCN (1 mM), glucose oxidase (10 units/mL), and glucose ($30{\mu}g/mL$). The final concentration of bLPO in the peroxidase and GO-PO systems ranged from 12.5 to $100{\mu}g/mL$. Candida albicans strains ATCC 10231, 11006, and 18804 were utilized. Candidacidal activities of antimicrobials and the influence of hyaluronic acid on their candidacidal activities were determined based on colony forming units. Results: Candidacidal activities of the peroxidase and GO-PO systems increased with increasing concentrations of bLPO. This tendency was the same in the presence or absence of hyaluronic acid. Candidacidal activity of HEWL was not significantly concentration-dependent. Candidacidal activities of the GO-PO system were higher than those of the corresponding peroxidase system. Candidacidal activity was inhibited in the presence of hyaluronic acid in the following order: HEWL, the peroxidase system, and the GO-PO system. Conclusions: Hyaluronic acid inhibited the candidacidal activities of HEWL, the peroxidase system, and the GO-PO system. The GO-PO system exhibited better candidacidal activity than HEWL and the peroxidase system both in the presence and absence of hyaluronic acid.