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Influences of Peroxidase on Lysozyme Activity  

Lee, Sang-Goo (Dept. of Oral Medicine & Oral Diagnosis, School of Dentistry & Dental Research Institute, Seoul National University)
Kim, Hyung-Il (Dept. of Oral Medicine & Oral Diagnosis, School of Dentistry & Dental Research Institute, Seoul National University)
Kho, Hong-Seop (Dept. of Oral Medicine & Oral Diagnosis, School of Dentistry & Dental Research Institute, Seoul National University)
Publication Information
Journal of Oral Medicine and Pain / v.33, no.1, 2008 , pp. 1-8 More about this Journal
Abstract
It is well known that many antimicrobial proteins in saliva interact with each other. The purpose of the present study was to investigate the interactions of lysozyme with peroxidase in the aspects of enzymatic activity in vitro. The interactions of lysozyme with peroxidase were examined by incubating hen egg-white lysozyme(HEWL) with bovine lactoperoxidase(bLP). The influence of peroxidase system on lysozyme was examined by subsequent addition of potassium thiocyanate and hydrogen peroxide. Lysozyme activity was determined by turbidity measurement of a Micrococcus lysodeikticus substrate suspension. Peroxidase activity was determined with an NbsSCN assay. The Wilcoxon signed rank test was used to analyze the changes of enzymatic activities compared with their controls. bLP at physiological concentrations enhanced the enzymatic activity of HEWL(P < 0.05) and its effect was dependent on the concentration of peroxidase. However, HEWL did not affect the enzymatic activity of bLP. Thiocyanate did not affect the enzymatic activity of HEWL, either. The addition of potassium thiocyanate and hydrogen peroxide did not lead to additional enhancement of the enzymatic activity of HEWL. The changes of hydrogen peroxide concentration in the peroxidase system did not affect the enzymatic activity of HEWL. Collectively, despite an in vitro nature of our study, the results of the present study provide valuable information on the interactions of lysozyme and peroxidase in the aspects of enzymatic activity in oral health care products and possibly in the oral cavity.
Keywords
Lysozyme; Peroxidase; Interaction;
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