• 농업과학연구
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• 제47권3호
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• pp.437-443
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• 2020

The aim of this study was to evaluate the effect of Lactobacillus acidophilus probiotic (LAP) product supplementation on the blood profile, fecal noxious gas emission, and fecal shedding of lactic acid bacteria and coliform bacteria in healthy adult Beagle dogs. In total, 14 Beagle dogs with an average initial body weight of 10.19 ± 0.61 kg were randomly assigned into two dietary treatments,with and without LAP supplementation, for a 28-day feeding trial. At the end of the experiment, there was no significant (p > 0.05) difference in the concentration of serum total cholesterol, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), white blood cell (WBC), red blood cell (RBC), blood lymphocyte percentage, fecal hydrogen sulfide (H₂S) and total mercaptans (R.SH) emission, and fecal coliforms counts. However, the serum concentrations of the triglyceride and fecal ammonia (NH₃) emission of the LAP treatment were significantly (p < 0.05) decreased in the group compared with the CON dogs. Fecal total lactic acid bacteria counts were significantly (p < 0.05) increased in the LAP treatment. In conclusion, the supplementation of LAP in Beagle dog diets could decrease the blood triglyceride level and enhance the gut Lactobacillus count which may have positive effects on dogs.

• 생명과학회지
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• 제20권11호
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• pp.1729-1737
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• 2010

본 연구는 양파즙을 이용하여 가축 사료첨가제용 Lactobacillus plantarum의 배양 최적화를 위하여 수행되었다. 양파즙의 첨가수준이 균체성장과 항산화 활성에 미치는 영향을 평가하였을 때, 그 첨가수준이 증가할수록 모두 높아졌다. 양파즙, 설탕 그리고 yeast extract를 배지성분요인으로 선택하였고, 배지 내 성분요인들의 혼합비율에 의한 효과를 평가하였다. 24 개의 실험들로 구성된 완전 요인 배치법을 이용하여 요인들의 주효과와 상호작용을 조사하였다. 균체성장과 항산화 활성에 대한 유의성 있는 긍정적 주효과는 각각 yeast extract와 양파즙에서 나타났다. 유의적인 상호작용으로는 항산화 활성에 대하여 설탕와 yeast extract 사이에서 나타났다. 최종적으로 양파 600 g/l, 설탕 15 g/l 및 효모추출물 5 g/l의 최적 배지조성을 선발하였다. 얻어진 최적배지의 효율을 분석하기 위하여 5 l 발효기를 이용하여 평가한 결과 최대 균체 성장량은 배양 12시간째에 $9.7\;{\log}_{10}$ (CFU/ml)로 나타났다. 배양 마지막인 20시간 때에는 $8.9\;{\log}_{10}$ (CFU/ml)의 균체 성장량을 보였으며 이는 예측된 $9.01\;{\log}_{10}$ (CFU/ml)과 매우 유사한 결과였다. 배양액의 항산화 활성은 모든 배양 시간대에서 약 60~65%로 유지되었으며, 이것은 예측된 값(47.1%)보다 훨씬 우수하였다.

• Journal of Dairy Science and Biotechnology
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• 제22권2호
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• pp.99-106
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• 2004

포자형성 유산균주에 대하여 종양세포에 대한 증식 억제 활성과 항산화 활성 및 세포내 glutathione 함유율을 측정하였다. 종양세포에 대한 억제활성은 균주에 따라 큰 차이를 나타내었고 Bacillus coagulans KTCC 3675는 높은 수준의 특이한 수준의 억제 활성을 나타내었고 human lymphoma cell NCLH-1299에 대한 억제 활성은 미약한 수준인 것으로 확인되었다. TBA 방법에 의한 linoleic acid에 대한 항산화 활성은 5~25% 정도의 항산화 활성을 보였고 특별히 높은 수준의 항산화 활성을 보인균주는 Bacillus coagulans KTCC 625, Bacillus coagulans KTCC 1015 및 Lactobacillus sporogens CU 815인 것으로 확인되었다. 포자형성 유산균체 추출액의 linoleic acid과산화 억제 활성과 세포내 glutathione함유율과의 상관계수는 0.78로서 고도의 유의성이 인정되는 상관계수를 나타내었다.

• Journal of Microbiology
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• 제42권4호
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• pp.315-318
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• 2004

This study was undertaken to determine the suitability of tomato juice as a raw material for production of probiotic juice by four lactic acid bacteria (Latobacillus acidophilus LA39, Lactobacillus plantarum C3, Lactobacillus casei A4, and Lactobacillus delbrueckii D7). Tomato juice was inoculated with a 24­h-old culture and incubated at $30^{\circ}C$. Changes in pH, acidity, sugar content, and viable cell counts dur­ing fermentation under controlled conditions were measured. The lactic acid cultures reduced the pH to 4.1 or below and increased the acidity to $0.65\%$ or higher, and the viable cell counts (CFU) reached nearly 1.0 to $9.0\times10^9/ml$ after 72 h fermentation. The viable cell counts of the four lactic acid bacteria in the fermented tomato juice ranged from $10^6\;to\;10^8\;CFU/ml$ after 4 weeks of cold storage at $4^{\circ}C$. Pro­biotic tomato juice could serve as a health beverage for vegetarians or consumers who are allergic to dairy products.

• 대한치과보철학회지
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• 제40권5호
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• pp.525-535
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• 2002

The purposes of this study were to evaluate the adherence of bacteria on various denture base resin materials and effects of chitosan, added to denture base materials on bacterial adherence. PMMA denture base resin such as heat-cured Vertex-RS, self-cured Vertex-SC and 4-META denture base resin such as heat-cured Meta-Dent, self-cured Meta-Fast were used in this study Samples were divided into two groups the denture base resin with chitosan, without chitosan Streptococcus mutans and Lactobacillus casei were used in this study. The surface of samples was observed by SEM. When chitosan was added to M17 and MRS broth, viable cell count of bacteria was reduced. Viable cell count of Streptococcus mutans on the samples decreased as follows : Meta-Dent, Vertex-SC, Meta-Fast, Vertex-RS. Viable cell count of Lactobacillus casei on the samples decreased as follows: Vertex-RS, Meta-Dent, Meta-Fast, Vertex-SC. The resin with chitosan showed lower adherence of bacteria than without chitosan. The images of SEM showed that the surface of the resin with chitosan was rougher than that of without chitosan. These results showed that the denture base resin materials with chitosan have rougher surface than without chitosan, but less bacteria adhered on them.

• Journal of Microbiology and Biotechnology
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• 제16권6호
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• pp.939-945
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• 2006

The physiological characteristics and function of the exopolysaccharide (EPS) produced by Lactobacillus rhamnosus ATCC 9595 were determined. The total quantity of EPS was rapidly increased to 496$\pm$20 mg/l during the exponential phase, and then maintained steadily during the stationary phase. During the exponential phase (18 h), the total EPS consisted of 61% cell-bound EPS (cb-EPS) and 39% released EPS (r-EPS), whereas the relative proportion of EPS during the stationary phase (48 h) was convered to 23% cb-EPS and 77% r-EPS. On gel permeation chromatography, cb-EPS was fractionated as a single peak of 8.6$\times10^6$ Da, whereas r-EPS was fractionated as two peaks with average molecular weights of 4.3$\times$10$^4$ and 8.6$\times10^6$ Da. Interestingly, both EPS species exhibited anticancer properties and cholera toxin-binding activities. Our results suggest that the EPS generated by L. rhamnosus ATCC 9595 might be suitable for use as a functional food or food supplement.

• Food Science and Biotechnology
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• 제15권6호
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• pp.877-883
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• 2006

The immunostimulatory activities of Lactobacillus plantarum, the major microorganism in kimchi fermentations were investigated. Five strains of L. plantarum exhibited weak immunopotentiating activity, but L. plantarum PS-21 showed as strong a mitogenic activity as Bifidobacterium adolescentis M101-4, a known positive strain. It is of interest that, L. plantarum PS-21 stimulated proliferation of Peyer's patch cells, one of the most important tissues in the gut-associated lymphoreticular system. Cell' wall fractions from L. plantarum PS-21 also showed strong mitogenic activity compared with the soluble cytoplasmic fraction. A peptidoglycan fraction (PG) extracted from the cell wall of L. plantarum PS-21 was identified as an active mitogenic component when used in murine lymph node and spleen cell test systems. PG showed dose-dependent mitogenic activity and significantly enhanced antibody production in lymph node cells when studied in vitro. The lysosomal enzyme activity of murine peritoneal macrophages was increased when analyzed following injection of PG to the host animal. Furthermore, PG enhanced the production of cytokines such ($TNF-{\alpha}$ and IL-6) in the in vitro culture of RAW 264.7 macrophage cells.

• 한국식품영양과학회지
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• 제19권1호
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• pp.94-106
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• 1990

사람들이나 동물의 소화관의 상재균으로서 중요한 Lactobacillus acidophilus의 여러균주를 사용하여 세포표면의 성상을 조사하였다. 불리한 세포벽의 negative 염색한 표본을 전자현미경으로 검토한 결과 사용한 21주중 15주는 세포벽외층에 단백질로 구성딘 regular array를 생산하고 있음을 알았다. RA를 구성하는 단백질의 분자량은 41KDa-48KDa이었다 아미노사노성 및 Staphylococcus au-reus V8 protease로 한정분해 후 및 N-chlorosuccini-mide로 부분절단후의 peptide map에서 Johnson등의 분류의 subgroup A-1에 속하는 균주가 생산하는 RA단백질은 불균질하였으나 그외 균주의 RA단백질은 같은 subgroup에 속하고 있어도 불균질하였음을 알았다 따라서 RA단백질은 불균질하였으나 그외 균주의 RA단백질은 같은 subgroup에 속하고 있어도 불균질하였음을 알았다 따라서 RA생산의 유무 및 RA단백질의 분자량측정과 peptide mapping을 행하면 지금까지 행해온 DNA-hybridization과 같은 복잡한 절차를 사용하지 않아도 subgroup A-1의 L. acidophilus가 동정 가능함을 알았다 L acidophilus의 세포표면소수성은 일반적으로 RA를 생산하지 않는 균주보다는 RA를 생사하는 균주에 높은 것이많았다 RA생산균주에 있어서는 RA단백질은 세포표면소수성과 직접관계하고 있는 것은 적었다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권2호
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• pp.128-132
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• 2001

Bacterial vaginosis can be treated by restoring the normal vaginal flora using lactobacilli. Lactobacillus crispatus KLB46 that was isolated from the human vagina has a string antimicrobial activity and was grown in a batch and in a continuous fermentor. During batch cultivation, the maximum specific growth rate of L. crispatus KLB 46 was 0.63h(sup)-1 and the highest viable cell count (1.9$\times$10(sup)9 CFU/mL) was obtained at pH 5.5. L. crispatus KLB 46 did not grow well at either pH 3.5 or 7.5. During continuous cultivation, the highest viable cell count (1.53$\times$10(sup)9 CFU/mL) was obtained at a dilution rate of 0.32h(sup)-1, and was 7.33$\times$10(sup)11 CFU L(sup)-1 h(sup)-1, that is approximately 5 times higher than that obtained from batch culture.

• KSBB Journal
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• 제18권1호
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• pp.14-18
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• 2003

본 연구에서는 제지 슬러지를 이용한 SSF 공정에 L..paracasei KLB58을 적용하여 젖산과 더불어 생균제용 균체를 경제적으로 대량 생산하고자 하였다. KLB58의 배양온도와 섬유소 가수분해 효소인 $\beta$-glucosidase의 농도를 조절하여 최적의 생산 조건을 확인해 본 결과, 37$^{\circ}C$ 에서 2 unit/ml의 $\beta$-glucosidase를 첨가하여 배양하였을 때 최대의 젖산과 균체를 생산하였다. 또한 $\beta$-glucosidase를 포함하지 않아도 상대적으로 많은 양의 젖산과 균체를 생산하였으므로, 이에 대한향후 연구가 기대된다.