• Journal of Chest Surgery
• /
• 제30권1호
• /
• pp.11-16
• /
• 1997

The Increasing use of coronary perfusates for the protection of the human heart during ischemic cardiac arrest has placed great emphasis on the need for a rational and safe formulation. For the purpose of this study isolated rat hearts were connected to retrograde nonworking perfusion system proposed by Langendorff, and then perfused for 20 minutes by coronary infusates of magnesium concentration of 1.66 m Mol per liter(group A, n: 10) or 15mMo1 per liter(group B, n: 10). After 20 minutes perfusion, cold cardioplegic solution (modified St. Thomas'Hospital solution) was infused for 2 minutes, and prepared within 4$^{\circ}C$ Krebs-Henseleit solution. Finally, 20 minutes of cononay reprsfuslon was reestablished after I hour of cold ischemic cardiac arrest. Hemodynamic parameters (heart rate, left ventricular pressure, $\pm$ dp/dt max. and coronany flow) and enzymes assay (creatine phosphokinase, lactic dehydrogenase and flutamic oxaloacetic transaminase) were performed each other at whic rat heart was perfused for 20 minutes and reperfused for 20 minutes thereafter. There were significant differences in the recovery rate of heart rate, systolic left ventricular pressure, + dp/dt max, and coronary flow and reperfusion-perfusion ratio of creatine phosphokinase(P < 0.05). But, there were no signicant differences in the recovery rate of dp/dt max, and reperfunion-perfusion ratio of lactic dehydrogenase and glutamic oxaloacetic acid (P > 0.05).

• Journal of Preventive Medicine and Public Health
• /
• 제16권1호
• /
• pp.79-88
• /
• 1983

There has been some evidence concerning the fact that trihalomethanes(THMs), toxic chlorinated compounds, may be present in drinking water. One of the important methodologies to evaluate the toxicity of THMs is to determine enzyme alteration in experimental animal tissues after treatment. This study was intended to investigate how lactic dehydrogenase(LDH) of rat tissues is affected by administration of chloroform($CHCl_3$) and dichloromonobromomethane($CHCl_2\;Br$). THMs, high dose(1/10 LD50) or low dose(1/50 LD50) of $CHCl_3$ or $CHCl_{2}Br$ were administered orally to experimental rats for 4 or 8 weeks. The treated groups of rats were sacrificed to determine LDH specific activity and isozyme pattern in various organs which were liver, thigh muscle, kidney and brain. The conclusions were obtained as follows: 1. Alteration of LDH activities and isozyme patterns were revealed before morphologic changes in tissues. 2. The LDH specific activities were increased significantly in liver and brain after administration of high concentrations of $CHCl_3$ and $CHCl_{2}Br$ for 4 weeks respectively. Otherwise, they were decreased significantly in liver, muscle and kidney after administration for 8 weeks. 3. The isozyme activities of LDH-4 and LDH-5 were increased in muscle, brain, and especially the liver. 4. It was more distinct for the decrement of LDH H-type isozyme than the increment of M-type isozyme in muscle.

• 미생물학회지
• /
• 제53권4호
• /
• pp.344-346
• /
• 2017

발효 식품에서 널리 발견되는 Lactobacillus plantarum은 환경에 적응하기 위한 다양한 표현형 및 유전적 특성을 가지고 있다. 우리는 사과 주스의 malolactic 발효를 위해 선발한 L. plantarum JBE245 (= KCCM43243) 유전체의 전체 염기서열과 주석을 보고한다. 유전체는 2907개의 암호화 된 영역, 45개의 위유전자, 91개의 RNA 유전자를 포함한 단일 원형 염색체로 구성되었으며 그 크기는 3,262,611 bp였다. 이 유전체는 4개의 malate dehydrogenase 및 3개의 malate permease 유전자들과 함께 다양한 종류의 plantaricins 합성 유전자들을 포함하고 있었다. 이러한 유전적 특성은 발효 동안 사과 주스의 부패 방지와 사과산(malate)의 젖산(lactate)으로의 효율적 전환이 요구되는 균주의 선발 기준과 잘 부합되었다.

• 한국산학기술학회논문지
• /
• 제18권2호
• /
• pp.218-225
• /
• 2017

본 연구에서는 알코올을 섭취한 흰쥐에서 알코올 대사와 혈액가스 불균형에 대한 꾸지뽕 나무 뿌리 추출물 (이하 CTE)의 효과를 확인하고자 하였다. 알코올을 10 ml/kg의 농도로 흰쥐에게 경구 투여하였고, 섭취 30분 후 CTE를 500 mg/kg 및 750 mg/kg의 농도로 투여하였다. 혈액은 알코올 섭취 1시간, 3시간, 5시간 후 꼬리 정맥을 통하여 채취하였다. 양성 대조군은 시판되고 있는 숙취해소 음료 (컨디션)를 사용하였다. 약물 처리는 모두 단회 경구 투여로 진행되었다. CTE의 섭취는 흰쥐의 혈액과 간 조직 내 알코올 대사 효소인 알코올 분해효소 (alcohol dehydrogenase)와 아세트알데히드 분해효소(acetaldehyde dehydrogenase)를 증가 또는 유지시킴으로써 혈중 알코올 농도를 감소시켰다. 또한, CTE는 알코올섭취에 의한 혈액가스 (pH, $CO_2$, $HCO_3{^-}$, lactic acid)의 불균형을 회복시켰다. 결론적으로, CTE는 현재 시판되고 있는 숙취해소음료 보다 우수한 숙취 해소 효과를 나타내었다. 따라서 본 연구자는 CTE를 숙취 해소 및 예방을 위한 천연물 유래의 안전하고 새로운 물질로써 제시하는 바이다.

• 약학회지
• /
• 제28권1호
• /
• pp.49-51
• /
• 1984

After pretreatment with ginseng followed by induction of acute intoxication of alcohol, the activities of alcohol dehydrogenase (ADH), microsomal ethanol-oxidizing system (MEOS) and aldehyde dehydrogenase(Ald DH) increased respectively compared to the groups treated with alcohol alone. In case that ginseng was given to rats fed with 5% alcohol instead of water for 60 days, the activities of ADH and MEOS increased compared to the groups treated. On the contrary, the activity of Ald DH in mitochondrial fraction decreased to an extent of about 35% in chronic alcoholism, but after pretreatment of ginseng the activity was restored to the control level. On the other hand, the catalase activity was not significantly affected by either treatment. Ginseng butanol fraction significantly increased the serum isocitrate dehydrogenase activity which is inhibited by alcohol-treated in rat. Alcohol-induced lactate dehydrogenase activity was decreased to control level in liver by ginseng treatment. And the serum level of lactic acid also decreased by ginseng treatment in alcohol-intoxicated rat. Ginseng butanol fraction markedly decreased the xanthine oxidase activity in the ethanol-treated rat liver. It was also observed that ginseng reduced the blood concentration of uric acid on experimentally reduced hyperuricemia by alcohol treatment. Uricase activity was not affected by either treatment. Ginseng butanol fraction decreased the hepatic aniline hydroxylase activity which was induced by alcohol-treated rat. These results suggest that the treatment with ginseng can be promoted the recovery from alcohol intoxication and some therapeutic effect on alcoholinduced metabolic disease.

• 미생물학회지
• /
• 제5권2호
• /
• pp.69-78
• /
• 1967

Kim, Jong Hyup, (Div. of Biology, Atomic Energy Research Institute.) Studies on the Cellular Metabolism in Microorganisms as influenced by Gamma-irradiation(II). On respiration rate and dehydrogenase activity of yeast cells irradiated by gamma ray from cobalt-60. 1. Oxygen uptake rate of the gamma irraiated yeast cells had been measured with Warburg's manometer, and the $O_{2}$-uptake was compared with those of normal cells. The rate of endogetious respiration increases in its $O_2$-uptake at 150, 000 rentgen dose, and at higher rentoen doses it was decreased. Exogenous respiration begin to decrease in its O_2$-uptake at 5, 000r. doses of irradiation, further decrease with increasing of doses unproportionally. 2. It appears that plasma-membrane and nuclear membrane of yeast cells have changed and denatured by gamma-irradiation, as exogenous respiration of glucose had been decreased at a dose of 200, 000r's irradiation. 3. The activity of glucose, alcoholic, lactic, succinic and glutamic deliydrogenase (G.D.H., A.D.H., L.D.H., S.D.11., and GL.D.H.) in the gamma irradaited cells had been assayed by T.T.C.(Triphenyl tetrazolium chloride) method and spectrophotometry, the obtained results were compared with those of normal cells. 4. At a dose of and 10, 000 rentgens' irradiation of gamma ray, the activty of each debydrogenase (G.D.H., A.D.H., L.D.H., ) shows a sharp and highest peak in optical absorbalicy, but each abtivity of S.D.H and Gl.D.H shows its' maximum peak at a dose of 30, 000r. 5. The curve of each dehydrogenase activity was found to be rhythmical according to dose-rate of gamma irradiation. 6. Comparing with activity of debydrogenase each other, the maximum peak in optical absorbency can be arranged according to order as follows; glucose > alcoholoic > lactic > glutamic > succinic, this order is identical to the order of breakdown utility in respiration of normal yeast cells. 7. The activity of dehydrogenase experimented exhibit a resistance against gamma irradiation at lethal dose of cells, and the activity of dehydrogenase are found to be much resistant than those of respiratory system. We may assume that the membrane substrate of mitochondria or cytoplasm had been destructed by gamma-irradiation much more than that of dehydronase system.

• 한국동물학회지
• /
• 제17권3호
• /
• pp.127-130
• /
• 1974

젖산수소이탈효소의 isozyme 형을 8종의 담수산 어류에서 전기영동법으로 조사하였다. 이 isozyme형의 전기영동상의 분포는 명백한 종 특이성을 보여 준다. 그리고 이 isozyme은 random hybridizaiton이 아니고 선택적인 hybridization에 의하여 구성되고 있음을 시사하고 있다. 포유류와 달라 어류에서는 근조직과 뇌조직의 isozyme형이 동일한 형을 나타내는 경향이 있다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제17권10호
• /
• pp.1411-1416
• /
• 2004

The objective of this work was to isolate a microorganism, able to produce high lactate dehydrogenase (LDH) activity, for use as a microbial feed additive. The LDH is an important enzyme for lactate conversion in the rumen, thereby possibly overcoming lactic acidosis owing to sudden increases of cereal in the diets of ruminants. In the present study, various bacterial strains were screened from a variety of environments. Among the isolated microorganisms, strain FFy 111-1 isolated from a Korean traditional fermented vegetable food called Kimchi showed the highest enzyme activity, along with retaining strong enzyme activity even in rumen fluid in vitro. Based on morphological and biochemical characteristics as well as compositions of cellular fatty acids plus API analyses, this strain was identified as Lactobacillus sp. The optimum temperature and pH for growth were found to be 30$^{\circ}C$ and pH 6.5, respectively. A maximum cell growth of 2.2 at $A_{650}$ together with LDH activity of 2.08 U per mL was achieved after 24 h of incubation. Initial characterization of FFy 111-1 suggested that it could be a potential candidate for use as a direct-fed microbial in the ruminant animals.

• 한국동물학회지
• /
• 제12권3호
• /
• pp.94-102
• /
• 1969

盛熱한 albino 雄性 흰쥐를 對照群과 實驗群으로 나누어 對照群에는 0.9% 生理食鹽水를, 實驗群에는 methylene blue(38mg/kg, 食鹽水에 溶解 pH 7.4)를 各各 腹腔注射處理하였다. 約 30 分後 兩群 共히 總線量 360 rads 인 $^60 Co$의 r 線을 一時 全身照射하였다. 照射後 212時間에 걸쳐 血淸總乳酸脫水素酵素의 含量(Cabaud and Droblewski 1958)과 血淸을 비롯하여 肝, 心臟, 精巢組織의 乳酸脫水素酵素의 同位酵素vnfortys(Preston et al., 1965)을 各各 測定하여 methylene blue 의 放射線에 對한 防禦　果를 硏究調査하였다. 一般的으로 血淸總乳酸脫水素酵素의 含量은 兩群共히 r 線照射後 初期에 增加하나 特히 methylene blue 實驗群인 處理群은 對照群인 生理食鹽水群에 比하여 15 및 64 時間區에서 增加의 遲滯性을 나타내었다. 血淸과 肝, 心臟 및 精巢組織의 乳酸脫水素酵素의 同位酵素는 兩群共히 線照射後 40과 116 時間區에서 陽極쪽의 同位酵素 푸랙숀 含量은 減少한다. 照射群 methylene blue 處理群의 血淸과 肝 및 精巢組織 乳酸脫水素酵素의 同位酵素패턴에서는 對照群과 別差異가 없었으나 特히 methylene blue 處理群의 心臟組織은 對照群에 比하여 若干의 增減의 遲滯性을 나타내었다. 兩群共히 照射後 血淸乳酸脫水素酵素含量의 增加는 同位酵素푸랙숀 1의 增加에 基因된다고 思料되며 이상의 結果로 미루어 보아 總乳酸脫水素酵素 活性과 心臟組織의 乳酸脫水素酵素의 同位酵素패턴에 있어서 methylene blue가 放射線防禦　果가 있는 것으로 思料된다.

• 대한추나의학회지
• /
• 제3권1호
• /
• pp.1-14
• /
• 2002

Objectives : To investigate the effects of Saengmaeksan-with-Ogapy on the recovery of fatigue induced by post-swimming exercise. Methods : The lactic acid, creatine phosphokinase(CPK), lactate dehydrogenase(LDH) and glucose were measured in blood. Results: The result were as follows; 1. The lactic acid levels in Saengmaeksan and Saengmaeksan-with-Ogapy group were immediately decreased at 30 minutes after exercise and statistically showed the significance different from the control group (P<0.005). 2. The CPK activities in Saengmaeksan and Saengmaeksan-with-Ogapy group were immediately decreased after exercise and statistically showed the significant difference compared with the control group (P<0.05). 3. The LDH activities in Saengmaeksan and Saengmaeksan-with-Ogapy group did not show the significant difference compared with the control group. 4. The glucose levels in Saengmaeksan and Saengmaeksan-with-Ogapy group did not statistically show the significant difference compared with the control group. Conclusions So it could be concluded that saengmaeksan and Saengmaeksan-with-Ogapy can recover the fatigue of after exercise.