• 동의생리병리학회지
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• 제19권5호
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• pp.1169-1180
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• 2005

First, I read all the materials, including Dongeuibogam(Encyclopedia Medica Koreana), classical writings on Lacca sinica exsiccata, herbal writings on lacquer poison, and herbal books on how to treat lacquer poison. And then after 1 examined all the details on qi and taste of Lacca sinica exsiccata, its efficacy, detoxification, lacquer poison, and its effects on body symptom, 1 got the following results. The order of frequency that Lacca sinica exsiccata is used in Dongeuibogam is pressure-feeling, blood circulation, and insect biting. Its way of intake is not so much through herb-boiling or powdered medicine as through hand-made pills. When medicine is used in the form of pills, the Lacca sinica exsiccata is more included among other ingredients. When old doctors treated pressure-feeling in the chest, they mixed up other herbs, with not putting more emphasis on the efficacy of lacquer 010 doctors believed that toxicity of Lacca sinica exsiccata is not having its own poison, but having biased dominance in the use of its qE and taste. The way or detoxification or Lacca sinica exsiccata is used in the order of crab-boiled water, egg, Xanthoxylum piperitum, Perilla frutescens, Astar tataricus, a weeping willow, iron-tempered water, and Allium toberosum. Special point in detoxificating lacquer poison is that they used medicines for well-ciruculating pulmonary stream, medicines for promoting to urinate or discharge by helping large colons to move, medicines for making the lacquer scar small, medicines for helping digest, and medicines for improving vessel function in the poisoned area. With the above results, the more profound study, based on the crab-boiled water and egg, is expected to go on to increase the effect on the one hand, and to make the new way of lessening or removing the toxicity of lacquer with more safe use on the other hand.

• 한방안이비인후피부과학회지
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• 제19권3호통권31호
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• pp.1-12
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• 2006

Objective : Angiogenesis is an essential process for metastasis of solid tumors and Psoriasis. Lots of Researches for anti-angiogenic effect to angiogenic factors have been carried out in the world. So this experiment was carried out for whether Lacca Sinica Exsiccata(LSE) extracts have an anti-angiogenic effect for angiogenic factors. Methods: To investigate the roles of the LSE extracts, we performed MIS assay, western blots using HaCaT cells and HepG2 cells. And then, HaCaT cells were treated with 10, 50, 100, 250, $500{\mu}g/ml$ LSE extracts. After 4hrs, HaCaT cells were theated with IGF-II protein for 1hr. HepG2 cells were treated with 1, 10, 25, 50, 100, 200 ${\mu}g/ml$ LSE extracts. After 4hrs, HepG2 cells were theated with $CoCl_2$ for 24hrs Results: 1. In $50{\mu}g/ml$ and $100{\mu}g/ml$ density we confirmed the inhibition effect of LSE extracts to $HIF-1{\alpha}$ activation which was induced by IGF-II in HaCaT cells. 2. In $50{\mu}g/ml$ density we confirmed the inhibition effect of LSE extracts to $HIF-1{\alpha}$ activation which was induced by $CoCl_2$ in HepG2 cells. 3. In $25{\mu}g/ml$ density we confirmed the inhibition effect of LSE extracts to VEGF activation which was induced by $CoCl_2$ in HepG2 cells. Conclusion: The above-mentioned results proved that LSE extracts reduced $HIF-1{\alpha}$ protein level in the HaCaT cells and HepG2 cells. These results suggest that inhibition of HaCaT cell and HepG2 cell proliferation by LSE extracts contributes to the anti-angiogenic activities on the keratinocytes and hepatocellular carcinoma.

• 대한본초학회지
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• 제35권3호
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• pp.9-16
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• 2020

Objectives : Sarcopenia is a disease that leads to a decrease in skeletal muscle, and the importance of prevention and treatment thereof is increasing in an aging society. However, there is a definite limitation of exercise therapy for sarcopenia, and thus, there is an urgent need for a pharmacologic research to the treatment of sarcopenia. Methods : 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay was performed to confirm the antioxidant efficacy of water extract of Lacca Sinica Exsiccata (WELSE). To determine the effect of WELSE on myoblast activity, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay was performed. To confirm the effect of WELSE on the differentiation of myoblast into myotubes, protein expression levels of myosin heavy chain 3 (Myh3) and paired box 3/7 (pax3/7) were confirmed by immunoblot analysis. In addition, immunofluorescence microscopy was performed to confirm the effect on myotube formation of WELSE. Results : It was confirmed that WELSE had high antioxidant activity and showed no cytotoxicity to myoblast up to 200 ㎍/㎖ concentration. Myoblast was treated with WELSE at a concentration of 100 ㎍/㎖ and differentiated for 5 days. The expression of Myh3, which forms myotubes, was promoted and the morphology of myotubes was changed and Increasing the thickness. Conclusions : In this paper, we confirmed the excellent antioxidant efficacy of WELSE and positive effects on muscle differentiation and myotube formation. These results suggest valuable as a material for pharmaceutical research on the prevention and treatment of sarcopenia.

• 한방안이비인후피부과학회지
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• 제22권1호
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• pp.33-45
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• 2009

Objective : Lacca Sinic: Exsiccate (LSE) extracted from Rhus vemicitlus Stokes (RVS) has been used traditionally as a remedy for inflammation in Korea, China, and Japan. However, as yet there is no clear explanation of how LSE affects the production of inflammatory cytokines. This study was to determine the effects of LSE on the mast cell-mediated inflammatory responses. Method : We measured the amount of inflammatory cytokine production induced by the phorbol myristate acetate (PMA) plus calcium ionophore(A231S7) in the human mast cell line (HMC-l) incubated with various concentrations of Laces Sinica Exsiccate (LSE). The $TNF-\alpha$, IL-6 and IL-8 secreted protein levels were measured by the ELISA assay. The $TNF-\alpha$, IL-6 and IL-8 mRNA levels were measured by the RT-PCR analysis. Nuclear and cytoplasmic proteins were examined by Western blot analysis. The NF-${\kappa}B$ promoter activity was examined by a luciferase assay. Result : LSE inhibited the PMA + A231S7-induced $TNF-\alpha$, IL-6, and IL-8 expression and suppressed NF-${\kappa}B$ activation in the stimulated-HMC-1. In addition, LSE inhibited induction of NF-${\kappa}B$ promoter-mediated luciferase activity. Conclusion : In this study, we have found that LSE is an inhibitor of NF-${\kappa}B$ and cytokines on the mast cell-mediated inflammatory responses.

• 대한본초학회지
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• 제25권2호
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• pp.21-39
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• 2010

Objectives & Method : We investigated toxicity, poisoning symptoms, poisoning treatment and prevention against poisoning of herbal medicines used to activate blood flow and remove blood stasis(活血祛瘀藥) in order to use herbal medicines accurately. Result : Cnidii Rhizoma(川芎), Olibanum(乳香), Myrrha(沒藥), Corydalis Tuber(玄胡索), Zedoariae Rhizoma(莪朮), Salviae Miltiorrhizae Radix(丹參), Polygoni Cuspidati Radix(虎杖根), Leonuri Herba(益母草), Persicae Semen(桃仁), Carthami Flos(紅花), Manitis Squama(穿山甲), Eupolyphaga(蟅蟲), Hirudo(水蛭), Vaccariae Semen(王不留行), Sappan Lignum(蘇木), Lacca Sinica Exsiccata(乾漆), Draconis Resina(血竭) and Leonuri Semen(茺蔚子) may give rise to some side effects or toxic symptoms in herbal medicines used to activate blood flow and remove blood stasis(活血祛瘀藥). The representative methods of poisoning treatment in western medicines are washing out the stomach, promotion of vomiting, causing diarrhea, supplies of grape sugar and symptomatic treatment, etc. The representative methods of poisoning treatment in oriental medicine take advantage of herbs. And Oriental medical doctor should meet symptoms as patients call for attention. In order to prevent against poisoning of herbal medicines used to activate blood flow and remove blood stasis (活血祛瘀藥), the patients should keep usage, dosage and notes and oriental medical doctors should do processing drugs. Conclusion : We should pay attention to clinical using of Cnidii Rhizoma(川芎), Olibanum(乳香), Myrrha(沒藥), Corydalis Tuber(玄胡索), Zedoariae Rhizoma(莪朮), Salviae Miltiorrhizae Radix(丹參), Polygoni Cuspidati Radix(虎杖根), Leonuri Herba(益母草), Persicae Semen(桃仁), Carthami Flos(紅花), Manitis Squama(穿山甲), Eupolyphaga(蟅蟲), Hirudo(水蛭), Vaccariae Semen(王不留行), Sappan Lignum(蘇木), Lacca Sinica Exsiccata(乾漆), Draconis Resina(血竭) and Leonuri Semen(茺蔚子) in herbal medicines used to activate blood flow and remove blood stasis(活血祛瘀藥).

• 대한예방한의학회지
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• 제10권1호
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• pp.75-93
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• 2006

Rhus verniciflua Stokes(RVS) has been widely used as a food and traditional herbal medicine in Korea. RVS has been reported that the extract from its wood and fruit has strong antioxidant activity and anticancer effect but there is little information on Lacca Sinica Exsiccata(LSE), the resin of RVS, as a medicinal use. The aim of this study was to evaluate the antidiabetic effect of ethanol-eluted extract of LSE on streptozotocin(STZ) - induced diabetic rats. Diabetes was induced in male Sprague-Dawley rats with STZ injection. Oral administration of LSE extract(50mg or 100mg/kg of body weight/day) was given to diabetic group. During 4 weeks of experiment, diabetic rats showed significant weight loss and decreasing feed efficiency ratios(FER) compared with normal rats, while the diabetic group orally fed with LSE extract showed a trend of decreasing weight loss and a significant increase of FER(p<0.05). In 4 weeks after induction of diabetes, diabetic rats showed an increase in weight of liver, kidney and heart, whereas the diabetic rats administered with LSE extract showed a reduction in the weight of heart. Blood glucose level was decreased in diabetic rats treated with LSE extract, but it was not statistically significant. Glutamic oxaloacetic transaminase, Glutamic pyruvate transaminase and total cholesterol levels were lower in the diabetic group treated with LSE extract than in untreated diabetic group, but not significant. These results present that LSE may partly have antidiabetic effect and may protect against the development of diabetic heart complications resulting from impaired glucose metabolism.