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http://dx.doi.org/10.6116/kjh.2020.35.3.9.

Investigation of the effect of Lacca Sinica Exsiccata water extract on myoblast differentiation  

Lee, Sangsoo (Department of Biochemistry, Chungnam National University)
Kim, Eun-Mi (Department of Predictive Toxicology, Korea Institute of Toxicology)
Cho, Namjoon (Department of Biochemistry, Chungnam National University)
Han, Hyosang (epartment of Health Administration, Joongbu University)
Kim, Kee Kwang (Department of Biochemistry, Chungnam National University)
Publication Information
The Korea Journal of Herbology / v.35, no.3, 2020 , pp. 9-16 More about this Journal
Abstract
Objectives : Sarcopenia is a disease that leads to a decrease in skeletal muscle, and the importance of prevention and treatment thereof is increasing in an aging society. However, there is a definite limitation of exercise therapy for sarcopenia, and thus, there is an urgent need for a pharmacologic research to the treatment of sarcopenia. Methods : 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay was performed to confirm the antioxidant efficacy of water extract of Lacca Sinica Exsiccata (WELSE). To determine the effect of WELSE on myoblast activity, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay was performed. To confirm the effect of WELSE on the differentiation of myoblast into myotubes, protein expression levels of myosin heavy chain 3 (Myh3) and paired box 3/7 (pax3/7) were confirmed by immunoblot analysis. In addition, immunofluorescence microscopy was performed to confirm the effect on myotube formation of WELSE. Results : It was confirmed that WELSE had high antioxidant activity and showed no cytotoxicity to myoblast up to 200 ㎍/㎖ concentration. Myoblast was treated with WELSE at a concentration of 100 ㎍/㎖ and differentiated for 5 days. The expression of Myh3, which forms myotubes, was promoted and the morphology of myotubes was changed and Increasing the thickness. Conclusions : In this paper, we confirmed the excellent antioxidant efficacy of WELSE and positive effects on muscle differentiation and myotube formation. These results suggest valuable as a material for pharmaceutical research on the prevention and treatment of sarcopenia.
Keywords
Lacca Sinica Exsiccata; Myoblast; sarcopenia; Myh3; myotube formation;
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