The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Investigation of the effect of Lacca Sinica Exsiccata water extract on myoblast differentiation

건칠(乾漆) 열수 추출물이 근원세포의 근분화에 미치는 영향

  • Lee, Sangsoo (Department of Biochemistry, Chungnam National University) ;
  • Kim, Eun-Mi (Department of Predictive Toxicology, Korea Institute of Toxicology) ;
  • Cho, Namjoon (Department of Biochemistry, Chungnam National University) ;
  • Han, Hyosang (epartment of Health Administration, Joongbu University) ;
  • Kim, Kee Kwang (Department of Biochemistry, Chungnam National University)
  • 이상수 (충남대학교 생화학과) ;
  • 김은미 (안전성평가연구소 예측독성연구본부) ;
  • 조남준 (충남대학교 생화학과) ;
  • 한효상 (중부대학교 보건행정학과) ;
  • 김기광 (충남대학교 생화학과)
  • Received : 2020.02.25
  • Accepted : 2020.05.25
  • Published : 2020.05.30
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Abstract

Objectives : Sarcopenia is a disease that leads to a decrease in skeletal muscle, and the importance of prevention and treatment thereof is increasing in an aging society. However, there is a definite limitation of exercise therapy for sarcopenia, and thus, there is an urgent need for a pharmacologic research to the treatment of sarcopenia. Methods : 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay was performed to confirm the antioxidant efficacy of water extract of Lacca Sinica Exsiccata (WELSE). To determine the effect of WELSE on myoblast activity, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay was performed. To confirm the effect of WELSE on the differentiation of myoblast into myotubes, protein expression levels of myosin heavy chain 3 (Myh3) and paired box 3/7 (pax3/7) were confirmed by immunoblot analysis. In addition, immunofluorescence microscopy was performed to confirm the effect on myotube formation of WELSE. Results : It was confirmed that WELSE had high antioxidant activity and showed no cytotoxicity to myoblast up to 200 ㎍/㎖ concentration. Myoblast was treated with WELSE at a concentration of 100 ㎍/㎖ and differentiated for 5 days. The expression of Myh3, which forms myotubes, was promoted and the morphology of myotubes was changed and Increasing the thickness. Conclusions : In this paper, we confirmed the excellent antioxidant efficacy of WELSE and positive effects on muscle differentiation and myotube formation. These results suggest valuable as a material for pharmaceutical research on the prevention and treatment of sarcopenia.

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References

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