• Title/Summary/Keyword: Laboratory tests

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Comparison of One-Tube Nested-PCR and PCR-Reverse Blot Hybridization Assays for Discrimination of Mycobacterium tuberculosis and Nontuberculous Mycobacterial Infection in FFPE tissues

  • Park, Sung-Bae;Park, Heechul;Bae, Jinyoung;Lee, Jiyoung;Kim, Ji-Hoi;Kang, Mi Ran;Lee, Dongsup;Park, Ji Young;Chang, Hee-Kyung;Kim, Sunghyun
    • Biomedical Science Letters
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    • v.25 no.4
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    • pp.426-430
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    • 2019
  • Currently, molecular diagnostic assays based on nucleic acid amplification tests have been shown to effectively detect mycobacterial infections in various types of specimen, however, variable sensitivity was shown in FFPE samples according to the kind of commercial kit used. The present study therefore used automated PCR-reverse blot hybridization assay (REBA) system, REBA Myco-ID HybREAD 480®, for the rapid identification of Mycobacterium species in various types of human tissue and compared the conventional one-tube nested-PCR assay for detecting Mycobacterium tuberculosis (MTB). In conventional nested-PCR tests, 25 samples (48%) were MTB positive and 27 samples (52%) were negative. In contrast, when conducted PCR-REBA assay, 11 samples (21%) were MTB positive, 20 samples (39%) were NTM positive, 8 samples (15%) were MTB-NTM double positive, and 13 samples (25%) were negative. To determine the accuracy and reliability of the two molecular diagnostic tests, the one-tube nested-PCR and PCR-REBA assays, were compared with histopathological diagnosis in discordant samples. When conducted nested-PCR assay, 10 samples (59%) were MTB positive and seven samples (41%) were negative. In contrast, when conducted PCR-REBA test, three samples (17%) were MTB positive, 10 samples (59%) were NTM positive and four samples (24%) were negative. In conclusion, the automated PCR-REBA system proved useful to identify Mycobacterium species more rapidly and with higher sensitivity and specificity than the conventional molecular assay, one-tube nested-PCR; it might therefore be the most suitable tool for identifying Mycobacterium species in various types of human tissue for precise and accurate diagnosis of mycobacterial infection.

A STUDY ON THE APPLICATION OF FIELD TESTS TO WEAK GROUND (연약지반에 대한 현장시험 적용에 관한 연구)

  • Lee, Seung-Rae;Jeong, Han-Jung;Kim, Jun-Seok
    • Proceedings of the Korean Geotechical Society Conference
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    • 1992.10a
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    • pp.145-148
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    • 1992
  • This paper reports the application of field tests to weak ground. In this study, field and laboratory tests were performed in the west seashore of KOREA(Ildo, Sore, Kunsan, Youngam). Applied tests are standard penetration test, cone penetration test, dilatometer test, field vane test, unconsolidated undrained test, oedometer test and other fundametal material properties tests. The results of in-situ test are used to estimate soil types and undrained shear strengths of five clay local deposits. SPT results showed low reliability and FVT war also demonstrated the best field test to weak clayey deposits.

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Comparison of Three Antibiotic Susceptibility Tests for Viridans Group Streptococci

  • Kim, Yeon-Hee;Lee, Si-Young
    • International Journal of Oral Biology
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    • v.36 no.4
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    • pp.163-166
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    • 2011
  • Oral viridans streptococci are recognized as one of the etiological agents of a variety of infectious diseases such as dental caries and infective endocarditis. Although antimicrobial susceptibility tests for these fastidious bacterial species are now established and standardized, a comparison between the broth microdilution and broth macrodilution tests has not previously been performed. This comparison was performed in the present study using the tests adopted by the Clinical and Laboratory Standards Institute (CLSI) and seven clinical isolates of oral viridans streptococcal strains. A modified broth macrodilution susceptibility test method was also included in this analysis, in which the media was not supplemented with horse blood. The susceptibility interpretation category agreements were measured at 83% (broth microdilution versus broth macrodilution) and 71% (broth microdilution versus modified broth macrodilution). The interpretation category agreement between the broth macrodilution and modified broth macrodilution tests was also 83%. These data indicate that the interpretation of antibiotic susceptibility test results for oral viridans streptococci are influenced by the methods used.

Laboratory experiment on the assessment of the ground strength with corestone (실내실험을 통한 핵석지반의 강도정수 산정연구)

  • 이수곤;김동은;황의성
    • Proceedings of the Korean Geotechical Society Conference
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    • 2003.03a
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    • pp.95-102
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    • 2003
  • Corestone rock mass has complex characters because it is made up of stronger and stiffer corestone in a weaker and softer matrix. Physical model corestone rock mass made up of stiffer corestone in weaker matrix were tested in uniaxial compression and numercal modelling analysis The result of the uniaxial compression tests showed that increasing the corestone proportion generally increased the modulus of deformation. And the strength decreased in the lower corestone proportion, but it increased in the higher proportion(45%, 65% corestone by volume). The strength and the modulus of deformation were not affected by different size coretone on the same proportion. The result of the numerical modelling analysis showed similar trend compared with the result of the result of the uniaxial compression test. But though the result of th uniaxial compression test is similar to the result of the numerical modelling analysis, it's unreasonalble to apply the results of this paper to in situ corestone rock mass. So mere laboratory tests including triaxial test and the other numerical program analyses are necessary to apply the results to in situ corestone mass

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Geotechnical Parameter Assessment for Tall Building Foundation Design

  • Poulos, Harry G.;Badelow, Frances
    • International Journal of High-Rise Buildings
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    • v.4 no.4
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    • pp.227-239
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    • 2015
  • This paper discusses the design parameters that are required for the design of high-rise building foundations, and suggests that the method of assessment for these parameters should be consistent with the level of complexity involved for various stages in the design process. Requirements for effective ground investigation are discussed, together with relevant in-situ and laboratory test techniques for deriving the necessary strength and stiffness parameters. Some empirical correlations are also presented to assist in the early stages of design, and to act as a check for parameters that are deduced from more detailed testing. Pile load testing is then discussed and a method of interpreting bi-directional tests to obtain pile design parameters is outlined. Examples of the application of the assessment process are described, including high-rise projects in Dubai and Saudi Arabia.

Investigation on Failure Mechanism of Geosynthethic Reinforced Slopes (보강토 구조물 파괴 메카니즘에 관한 연구)

  • Yoo, Chung-Sik;Kim, Ki-Yeon;Lee, Sung-Woo;Lee, Bong-Won
    • Journal of the Korean Geosynthetics Society
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    • v.4 no.2
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    • pp.19-28
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    • 2005
  • This paper presents a laboratory investigation on the failure mechanism of geosynthetic reinforced slopes using 1/5-scale reduced model. The components of the model were selected with due consideration of the similitude law and the step-by-step actual wall construction procedure was closely simulated. The model tests successfully replicated the failure mechanism with relative density, slope and vertical spacing.

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Development of Geotube for Practical Use (지오튜브공법의 실용화방안에 관한 연구)

  • 신은철;오영인;이희재
    • Proceedings of the Korean Geotechical Society Conference
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    • 2000.03b
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    • pp.504-511
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    • 2000
  • Geotube is a tube made of permeable but soil-tight geotextile, hydraulically filled with soil include dredged sand and mud, which has been successfully applied in hydraulic and coastal engineering projects. This method is getting popular and used a lot in the advanced countries of the world because of economical, useful, and enable to store and isolate contaminated materials as obtained by harbor dredging. Laboratory and pilot scale in-situ tests were performed to determine the design methodology and construction procedures. From the results of laboratory and in-situ model tests, the retention ratio of solid particle is minimum 86% and minimum permeability and tensile strength of geotextile is ${\alpha}$${\times}$10$\^$-2/ and 20 t/m, respectively. Also, based on the environmental model test results, it can be concluded that this method does meet the Korean EPA standards.

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Evaluation of Seismic Response Characteristics of Hong-Seong Area based on In-Situ and Laboratory Tests (현장 및 실내시험에 기초한 홍성지역 지진응답특성 평가)

  • 박덕근;김교원
    • The Journal of Engineering Geology
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    • v.11 no.1
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    • pp.25-35
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    • 2001
  • For the ground resrxmse analysis, both in-situ and laboratory testing techniques such as downhole, SASW, resonant column and torsional shear tests were perlormed for Hong-Seong area. The grOlmd upper 30m is classified as SD since it has an average shear wave velocity as 209m/s. The response specLrums obtained by site-specific analyses generally satisfied the seismic code, but near the resonance period the motion was evaluated to be higher than the code.

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A decision support system the interface between PACS and Laboratory Information (PACS 와 임상검사정보의 연동으로 인한 의사결정시스템; 크레아틴 수치정보전송으로 인한 조영제 부작용 예방)

  • Kim, Sean-Chil;Cho, Hune
    • Korean Journal of Digital Imaging in Medicine
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    • v.9 no.1
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    • pp.17-19
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    • 2007
  • This study applies in case of operating an exam using by the contrast order or inputting an order of a contrast media the exam of Radiology Department. It is developed for helping decision making as regards a process of an exam from reading the creatinine value automatically linked with Laboratory Information System. It can be confirmed by real-time information; therefore, the creditability of the information is able to be improved. We will create the base for Patient Monitoring System with the data from the side effect of the creatinine value and allergies. Decision Support System minimize the inconvenience and the riskiness of the given contrast medium for CT tests. We would like to improve medical services by providing a standard circumstance where patients are able to run tests safely and comfortably.

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Surface Subsidence according to Progressive Collapse of Circular opening (원형공동의 점진적인 붕락에 따른 지표침하특성)

  • 지정배;김종우
    • Tunnel and Underground Space
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    • v.10 no.1
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    • pp.33-44
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    • 2000
  • In order to investigate the effect of progressive collapse of underground circular opening on surface subsidence, laboratory model tests were performed. The modelling materials were sand which has been used as KS standard. Six test models which had respectively different depths of openings were produced. Surface subsidence and horizontal displacements were measured according to progressive collapse of underground opening. Some subsidence prediction method such as NCB method, profile function method and influence function method were considered to predict the subsidence of sand models. The profile function method approximated by Gaussian error function was finally suggested as the most appropriate to sand models.

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