• Title/Summary/Keyword: Laboratory and field culture

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Penicillium koreense sp. nov., Isolated from Various Soils in Korea

  • You, Young-Hyun;Cho, Hye Sun;Song, Jaekyeong;Kim, Dae-Ho;Houbraken, Jos;Hong, Seung-Beom
    • Journal of Microbiology and Biotechnology
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    • v.24 no.12
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    • pp.1606-1608
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    • 2014
  • During an investigation of the fungal diversity of Korean soils, four Penicillium strains could not be assigned to any described species. The strains formed monoverticillate conidiophores with occasionally a divaricate branch. The conidia were smooth or finely rough-walled, globose to broadly ellipsoidal and $2.5-3.5{\times}2.0-3.0{\mu}m$ size. Their taxonomic novelty was determined using partial ${\beta}$-tubulin gene sequences and the ribosomal internal transcribed spacer region. The phylogenetic analysis showed that the isolates belonged to section Lanata-Divaricata and were most closely related to Penicillium raperi. Phenotypically, the strains differed from P. raperi in having longer and thicker stipes and thicker phialides. Strain KACC $47721^T$ from bamboo field soil was designated as the type strain of the new species, and the species was named Penicillium koreense sp. nov., as it was isolated from various regions in Korea.

A Study on Experimental Clothing of the Early 20th Century Italian Artists (20세기 초 이탈리아의 실험예술 의상에 관한 연구)

  • 이금희
    • The Research Journal of the Costume Culture
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    • v.9 no.1
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    • pp.111-126
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    • 2001
  • This study concentrates on the relationship between the early 20th century italian artists and their works in the field of clothing design. They advocated the creation of art for life and introduced a new type of work of art which I will call 'experimental clothing for art'. The experimental clothing for art showed its dynamic characteristics in the field s of line and form, color, pattern, and material. The Italian artists made simple and functionalistic dresses, using asymmetric, geometric cuts. in pattern making. They employed dynamic patterns in textile design and favored brilliant colors which they debunked as storage and traditional. With regard to material, they used unusual materials such as metal, net, wire, and paper and inexpensive materials. To investigations of the visual expression of experimental clothing for art in Italy have led us to the internal expressions which are avant-garde, dynamic & speed, functionality & popularity, ephemeral & transformable, and warlike. As a result of the reflection of the times and the artists's will and roles the experimental clothing for art in Italy implicated contemporary clothing in the early twentieth century and it was only laboratory art that underwent various experiments in canvas but a model of efforts for the at of living, which was anti-traditional. It offered a new future and created a new environment. It is left for future research how the experimental clothing for art developed in countries other than Italian.

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Recurrent Pseudomonas aeruginosa Infection in Chronic Lung Diseases: Relapse or Reinfection?

  • Yum, Ho-Kee;Park, I-Nae;Shin, Bo-Mun;Choi, Soo-Jeon
    • Tuberculosis and Respiratory Diseases
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    • v.77 no.4
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    • pp.172-177
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    • 2014
  • Background: Pseudomonas aeruginosa infection is particularly associated with progressive and ultimately chronic recurrent respiratory infections in chronic obstructive pulmonary disease, bronchiectasis, chronic destroyed lung disease, and cystic fibrosis. Its treatment is also very complex because of drug resistance and recurrence. Methods: Forty eight cultures from 18 patients with recurrent P. aeruginosa pneumonia from 1998 to 2002 were included in this study. Two or more pairs of sputum cultures were performed during 2 or more different periods of recurrences. The comparison of strains was made according to the phenotypic patterns of antibiotic resistance and chromosomal fingerprinting by pulsed field gel electrophoresis (PFGE) using the genomic DNA of P. aeruginosa from the sputum culture. Results: Phenotypic patterns of antibiotic resistance of P. aeruginosa were not correlated with their prior antibiotic exposition. Fifteen of 18 patients (83.3%) had recurrent P. aeruginosa pneumonia caused by the strains with same PFGE pattern. Conclusion: These data suggest that the most of the recurrent P. aeruginosa infections in chronic lung disease occurred due to the relapse of prior infections. Further investigations should be performed for assessing the molecular mechanisms of the persistent colonization and for determining how to eradicate clonal persistence of P. aeruginosa.

Parametric Study on Track Deterioration by Various Track Type of Serviced Line (운행선 궤도형식별 궤도열화에 미치는 매개변수 연구)

  • Choi, Jung-Youl;Park, Jong-Yoon;Chung, Jee-Seung
    • The Journal of the Convergence on Culture Technology
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    • v.8 no.3
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    • pp.239-244
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    • 2022
  • In this study, the key parameters affecting the deterioration of each track type were derived based on field inspections and laboratory tests. The existing track deterioration model was limited to the ballasted tracks, and the deterioration evaluation of concrete tracks was insufficient. In this study, the laboratory test was performed to evaluate the performance and condition of track components to derive the deterioration factors reflecting the characteristics of various track structures. In addition, through analysis of track maintenance history data, parameters affecting track deterioration and maintenance were derived. The key parameters for presenting a track deterioration model based on the track performance of ballasted and concrete tracks through field inspection, track maintenance history data analysis, and performance test of track components using on-site specimens were identified as track support stiffness, Ballast gravel, track settlement and Resilience pad were presented.

Studies on Sheath Rot of Rice Caused by Acrocylindrium oryzae Sawada -Testing Varietal Reactions and Culture Filtrates of the Causal Fungus - (벼 잎집썩음병에 관한 연구 -품종저항성검정, 병균의 배양여액-)

  • Chung Hoo Sup
    • Korean journal of applied entomology
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    • v.14 no.1 s.22
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    • pp.23-27
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    • 1975
  • Occurrence of sheath rot rice caused by Acrocylindrium oryzae on indica varieties, IR lines and IR varieties was light to moderate in the field, whereas generally none of the recommended japonica varieties showed the disease. In general, percentage infection in the field agreed with that obtained from seed inoculation tests made in the laboratory. Culture filtrates of the fungus inhibited the sprouting of seeds of rice, barley, wheat, rye and rape. A new variety, Tongil obtained from a hybrid between japonica and indica was inhibited significantly more than was Jinheung. Conidial germination of Pyricularia oryzae was inhibited by the filtrate while no inhibitory effects were found on conidia of Cochliobolus miyabeanus.

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Recent R&D on Oxide Scintillation Crystals for Radiation Detectors

  • Ishii, M.;Kobayashi, M.;Hara, K.;Tanaka, M.;Yamaga, I.;Miwa, K.;Ishibashi, H.;Usuki, Y.;Hirose, Y.
    • Proceedings of the Korea Association of Crystal Growth Conference
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    • 1997.06a
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    • pp.131-134
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    • 1997
  • Scintillation crystals for industrial field are used in fundamental physics i.e. nuclear and high energy physics experiments besides the medical imaging, process control and gauging, container inspection, mineral process etc. For the reason of limited marketability, there are not so many studies with emphasis paced on the crystal growth. The scintillation crystal is an important theme in the studies in the fundamental physics and researchers for crystal growth are expected participate it. The present work is partly supported by a Grant-in-Aid from the Japanese Ministry of Education, Science and Culture.

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Study on application case of reinforce building shallow foundation for soil stabilized materials using circulating resources (순환자원 활용 지반안정재의 건축물 얕은기초 보강 적용사례 연구)

  • Song, Sang-Huwon
    • The Journal of the Convergence on Culture Technology
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    • v.8 no.3
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    • pp.457-462
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    • 2022
  • This study is about the case applied of a shallow foundation reinforcement method for a low/mid-rise building where a relatively small load is applied by using a soil stabilized material that utilizes recycled resources. First, laboratory mixing test was conducted for four mixing ratios in order to derive the optimal mixing ratio in the field. Using the derived optimal mixing ratio, it was applied as a shallow foundation for the building in the field. The field application method used a simple process of compaction by the soil mixedure with the original soil and the soil stabilized material in the field. After field application, a plate bearing test was performed on one original ground and two improved ground to confirm the allowable bearing capacity. As a result of checking the bearing capacity, it was found that sufficient bearing capacity was exhibited.Therefore, it was confirmed that it can be used as a shallow foundation for the building.

Field and Culture Studies on the Growth and Reproduction of Campylaephora hypnaeoides (석묵 (Campylaephora hypnaeoides)의 생장과 성숙에 대한 야외 및 배양 연구)

  • Yoo, Hyun-Il;Kim, Ji-Hwan;Choi, Han-Gil
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.44 no.3
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    • pp.290-297
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    • 2011
  • The phenology of Campylaephora hypnaeoides J. Agardh and optimal conditions for carpospore release, growth and reproduction were examined in the field and in the laboratory from January to December 2007. In the field population of C. hypnaeoides, approximately 50% of the plants were vegetative during the study period. Additionally, the percentages of carposporophytes and tetrasporophytes were maximal in April (37%) and June (57%), respectively. Maximum growth in plant length, dry weight, and hook number coincided with the tetrasporophyte reproductive peak in the field. In culture, carpospore release, sporeling growth and reproduction were affected by environmental factors such as daylength, temperature, and salinity. The liberation of carpospores was maximum under continuous light and at a combination of $15^{\circ}C$ and $10\;{\mu}mol$ photons $m^{-2}\;s^{-1}$. Maximum growth of tetrasporophyte sporelings occurred at a combination of $20\;{\mu}mol$ photons $m^{-2}\;s^{-1}$ of constant light and $25^{\circ}C$. However, the growth of gametophyte sporelings was maximal under $40\;{\mu}mol$ photons $m^{-2}\;s^{-1}$ of constant light and in a combination of $20^{\circ}C$ and 35 psu. The tetrasporophyte sporelings were grew faster than gametophytes, indicating that gametophyte- and tetrasporophyte-sporelings have different physiological responses to irradiance and temperature. Tetrasporangial branches and cystocarps of C. hypnaeoides were produced from carpospores and tetraspores within 1 month, and they were stimulated at high temperature and irradiance levels. In conclusion, C. hypnaeoides should be seeded using carpospores during early winter (November-December) because cystocarps are easily identified by fishermen, and tetrasporophytes grow faster than gametophytes.

Identification of Cochlodinium polykrikoides against Gyrodinium impudicum and Gymnodinium catenatum in Field Samples using FITC Lectin Probes

  • Cho Eun Seob;Kang Dong Woo;Cho Yong Chul
    • Fisheries and Aquatic Sciences
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    • v.3 no.2
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    • pp.83-87
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    • 2000
  • We have investigated lectin binding patterns in order to apply binding records of previous laboratory experiments to field settings before the first ourbreaks of harmful algal bloom (HAB). Although cells were grown under different conditions, the binding patterns were the same as in the control. In addition, culture days was not associated with the binding patterns, when compared with the control. In nature, this results suggest that ECA, HPA and WGA lectin are able to discriminate between C. polykrikoides and G. impudicum, as well as ECA and SBA have a capability as a tool for differentiating between C. polyrikoides and G. catenatum, although these species are closely similar under the light microscope fiexed with Lugol solution.

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The Cultural and Environmental Unsoundness of the Chinese Public Squatting-Type Toilet: A Case Study toward a Sustainable Excreta Treatment System

  • Chang, Jin-Soo
    • Environmental Engineering Research
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    • v.19 no.2
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    • pp.131-138
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    • 2014
  • The inconvenient truth of sustainable public squat toilet culture varies among nationalities. This culture may be comfortable to the people of China, yet uncomfortable to the non-Chinese, according to the adequate environmental management in Yanbian Korean Autonomous Prefecture (YKAP), northern China. We conducted a series of field surveys and individual interviews (Chinese n = 1,000 and non-Chinese [foreign visitors] n = 100) on several aspects of the public squat toilet: structural properties, waste disposal methods, important factors, and overall satisfaction level. The significant factors in response to the public squat toilets were cleanliness, odor, toilet paper, temperature, soap, other facilities, and presence of cubicles. These factors should be policy priorities of the local government. In addition, 66.2% of Chinese and 91% of foreign visitors desired type E toilets (two full-high partition walls and a door). The results illustrate the nature of a sustainable and beautiful approach to the culturally and environmentally sound management of various types of public squat toilet in YKAP. The government needs to focus on the future-oriented and excreta treatment management of the sustainable toilet culture for residents and visitors of YKAP.