• 제목/요약/키워드: Laboratory and field culture

검색결과 83건 처리시간 0.021초

Penicillium koreense sp. nov., Isolated from Various Soils in Korea

  • You, Young-Hyun;Cho, Hye Sun;Song, Jaekyeong;Kim, Dae-Ho;Houbraken, Jos;Hong, Seung-Beom
    • Journal of Microbiology and Biotechnology
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    • 제24권12호
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    • pp.1606-1608
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    • 2014
  • During an investigation of the fungal diversity of Korean soils, four Penicillium strains could not be assigned to any described species. The strains formed monoverticillate conidiophores with occasionally a divaricate branch. The conidia were smooth or finely rough-walled, globose to broadly ellipsoidal and $2.5-3.5{\times}2.0-3.0{\mu}m$ size. Their taxonomic novelty was determined using partial ${\beta}$-tubulin gene sequences and the ribosomal internal transcribed spacer region. The phylogenetic analysis showed that the isolates belonged to section Lanata-Divaricata and were most closely related to Penicillium raperi. Phenotypically, the strains differed from P. raperi in having longer and thicker stipes and thicker phialides. Strain KACC $47721^T$ from bamboo field soil was designated as the type strain of the new species, and the species was named Penicillium koreense sp. nov., as it was isolated from various regions in Korea.

20세기 초 이탈리아의 실험예술 의상에 관한 연구 (A Study on Experimental Clothing of the Early 20th Century Italian Artists)

  • 이금희
    • 복식문화연구
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    • 제9권1호
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    • pp.111-126
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    • 2001
  • This study concentrates on the relationship between the early 20th century italian artists and their works in the field of clothing design. They advocated the creation of art for life and introduced a new type of work of art which I will call 'experimental clothing for art'. The experimental clothing for art showed its dynamic characteristics in the field s of line and form, color, pattern, and material. The Italian artists made simple and functionalistic dresses, using asymmetric, geometric cuts. in pattern making. They employed dynamic patterns in textile design and favored brilliant colors which they debunked as storage and traditional. With regard to material, they used unusual materials such as metal, net, wire, and paper and inexpensive materials. To investigations of the visual expression of experimental clothing for art in Italy have led us to the internal expressions which are avant-garde, dynamic & speed, functionality & popularity, ephemeral & transformable, and warlike. As a result of the reflection of the times and the artists's will and roles the experimental clothing for art in Italy implicated contemporary clothing in the early twentieth century and it was only laboratory art that underwent various experiments in canvas but a model of efforts for the at of living, which was anti-traditional. It offered a new future and created a new environment. It is left for future research how the experimental clothing for art developed in countries other than Italian.

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Recurrent Pseudomonas aeruginosa Infection in Chronic Lung Diseases: Relapse or Reinfection?

  • Yum, Ho-Kee;Park, I-Nae;Shin, Bo-Mun;Choi, Soo-Jeon
    • Tuberculosis and Respiratory Diseases
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    • 제77권4호
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    • pp.172-177
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    • 2014
  • Background: Pseudomonas aeruginosa infection is particularly associated with progressive and ultimately chronic recurrent respiratory infections in chronic obstructive pulmonary disease, bronchiectasis, chronic destroyed lung disease, and cystic fibrosis. Its treatment is also very complex because of drug resistance and recurrence. Methods: Forty eight cultures from 18 patients with recurrent P. aeruginosa pneumonia from 1998 to 2002 were included in this study. Two or more pairs of sputum cultures were performed during 2 or more different periods of recurrences. The comparison of strains was made according to the phenotypic patterns of antibiotic resistance and chromosomal fingerprinting by pulsed field gel electrophoresis (PFGE) using the genomic DNA of P. aeruginosa from the sputum culture. Results: Phenotypic patterns of antibiotic resistance of P. aeruginosa were not correlated with their prior antibiotic exposition. Fifteen of 18 patients (83.3%) had recurrent P. aeruginosa pneumonia caused by the strains with same PFGE pattern. Conclusion: These data suggest that the most of the recurrent P. aeruginosa infections in chronic lung disease occurred due to the relapse of prior infections. Further investigations should be performed for assessing the molecular mechanisms of the persistent colonization and for determining how to eradicate clonal persistence of P. aeruginosa.

운행선 궤도형식별 궤도열화에 미치는 매개변수 연구 (Parametric Study on Track Deterioration by Various Track Type of Serviced Line)

  • 최정열;박종윤;정지승
    • 문화기술의 융합
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    • 제8권3호
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    • pp.239-244
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    • 2022
  • 본 연구는 현장조사 및 실내시험을 바탕으로 궤도형식별 열화에 영향을 미치는 핵심매개변수를 도출하였다. 기존 궤도 열화모델은 자갈궤도에 국한된 모델로서 콘크리트궤도의 열화평가는 연구된 것이 없는 실정이다. 본 연구에서는 운행선 궤도형식별 다양한 궤도구조의 특성이 반영된 열화요인을 도출하고자 궤도구성품의 성능수준 및 상태평가를 위한 실내시험을 수행하였다. 또한 궤도유지관리 이력데이터에 대한 분석을 통해 궤도열화 및 유지관리에 영향을 미치는 매개변수를 도출하였다. 현장조사, 궤도유지관리 이력데이터 분석 및 현장시료를 이용한 궤도구성품의 성능시험을 통해 궤도성능기반의 궤도열화 매개변수는 궤도침하 및 궤도지지강성에 직접적인 영향을 미칠 수 있는 도상자갈과 방진패드인 것으로 분석되었다.

벼 잎집썩음병에 관한 연구 -품종저항성검정, 병균의 배양여액- (Studies on Sheath Rot of Rice Caused by Acrocylindrium oryzae Sawada -Testing Varietal Reactions and Culture Filtrates of the Causal Fungus -)

  • 정후섭
    • 한국응용곤충학회지
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    • 제14권1호
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    • pp.23-27
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    • 1975
  • 1. 포장에서 벼, 잎집썩음병 이병율은 일본형장려품종에는 거의 전무였으나 대체로 인도형품종 및 IR계통 또는 품종은 경-중도였다. 모든 공시품종 또는 계통은 종자에 접종하면 감염되었다. 실내에서 종자접한 결과는 몇 예외를 제외하고 포장에서의 이병율과 대체로 비슷하였다. 2. 벼, 잎집썩음병균의 배양여액은 벼, 보리, 밀, 호밀, 유채의 초엽 및 유근의 생육을 억제하였다. 특히 벼품종 통일은 진흥보다 현저히 초엽 및 유근의 생육이 억제되었다. 3. 병균 배양여액은 도열병균의 포자발아를 억제하였으나 깨씨무늬병균에는 영향이 없었다.

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Recent R&D on Oxide Scintillation Crystals for Radiation Detectors

  • Ishii, M.;Kobayashi, M.;Hara, K.;Tanaka, M.;Yamaga, I.;Miwa, K.;Ishibashi, H.;Usuki, Y.;Hirose, Y.
    • 한국결정성장학회:학술대회논문집
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    • 한국결정성장학회 1997년도 Proceedings of the 12th KACG Technical Meeting and the 4th Korea-Japan EMGS (Electronic Materials Growth Symposium)
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    • pp.131-134
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    • 1997
  • Scintillation crystals for industrial field are used in fundamental physics i.e. nuclear and high energy physics experiments besides the medical imaging, process control and gauging, container inspection, mineral process etc. For the reason of limited marketability, there are not so many studies with emphasis paced on the crystal growth. The scintillation crystal is an important theme in the studies in the fundamental physics and researchers for crystal growth are expected participate it. The present work is partly supported by a Grant-in-Aid from the Japanese Ministry of Education, Science and Culture.

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순환자원 활용 지반안정재의 건축물 얕은기초 보강 적용사례 연구 (Study on application case of reinforce building shallow foundation for soil stabilized materials using circulating resources)

  • 송상훤
    • 문화기술의 융합
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    • 제8권3호
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    • pp.457-462
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    • 2022
  • 본 연구는 순환자원을 대량 활용한 지반안정재를 활용하여, 비교적 작은 하중이 작용하는 중·저층 건축물의 얕은 기초 보강공법에 적용한 사례에 대한 내용으로 우선하여 현장 최적배합비를 도출하고자 4가지 혼합비에 대한 실내배합시험을 실시하였으며, 도출된 최적배합비를 이용하여 현장에서 건축물 얕은기초로 적용하였다. 현장 적용방법은 원지반와 지반안정재를 교반한 혼합토를 현장에서 혼합하여 다짐을 실시하는 매우 단순한 공정을 이용하였다. 현장 적용 후 평판재하시험을 원지반 1곳, 개량지반 2곳에 실시하여 허용지지력을 확인하였다. 지지력 확인 결과 충분한 지지력을 발휘하는 것으로 나타나, 해당 건축물의 앝은기초로 활용이 가능한 것으로 확인되었다.

석묵 (Campylaephora hypnaeoides)의 생장과 성숙에 대한 야외 및 배양 연구 (Field and Culture Studies on the Growth and Reproduction of Campylaephora hypnaeoides)

  • 유현일;김지환;최한길
    • 한국수산과학회지
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    • 제44권3호
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    • pp.290-297
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    • 2011
  • The phenology of Campylaephora hypnaeoides J. Agardh and optimal conditions for carpospore release, growth and reproduction were examined in the field and in the laboratory from January to December 2007. In the field population of C. hypnaeoides, approximately 50% of the plants were vegetative during the study period. Additionally, the percentages of carposporophytes and tetrasporophytes were maximal in April (37%) and June (57%), respectively. Maximum growth in plant length, dry weight, and hook number coincided with the tetrasporophyte reproductive peak in the field. In culture, carpospore release, sporeling growth and reproduction were affected by environmental factors such as daylength, temperature, and salinity. The liberation of carpospores was maximum under continuous light and at a combination of $15^{\circ}C$ and $10\;{\mu}mol$ photons $m^{-2}\;s^{-1}$. Maximum growth of tetrasporophyte sporelings occurred at a combination of $20\;{\mu}mol$ photons $m^{-2}\;s^{-1}$ of constant light and $25^{\circ}C$. However, the growth of gametophyte sporelings was maximal under $40\;{\mu}mol$ photons $m^{-2}\;s^{-1}$ of constant light and in a combination of $20^{\circ}C$ and 35 psu. The tetrasporophyte sporelings were grew faster than gametophytes, indicating that gametophyte- and tetrasporophyte-sporelings have different physiological responses to irradiance and temperature. Tetrasporangial branches and cystocarps of C. hypnaeoides were produced from carpospores and tetraspores within 1 month, and they were stimulated at high temperature and irradiance levels. In conclusion, C. hypnaeoides should be seeded using carpospores during early winter (November-December) because cystocarps are easily identified by fishermen, and tetrasporophytes grow faster than gametophytes.

Identification of Cochlodinium polykrikoides against Gyrodinium impudicum and Gymnodinium catenatum in Field Samples using FITC Lectin Probes

  • Cho Eun Seob;Kang Dong Woo;Cho Yong Chul
    • Fisheries and Aquatic Sciences
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    • 제3권2호
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    • pp.83-87
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    • 2000
  • We have investigated lectin binding patterns in order to apply binding records of previous laboratory experiments to field settings before the first ourbreaks of harmful algal bloom (HAB). Although cells were grown under different conditions, the binding patterns were the same as in the control. In addition, culture days was not associated with the binding patterns, when compared with the control. In nature, this results suggest that ECA, HPA and WGA lectin are able to discriminate between C. polykrikoides and G. impudicum, as well as ECA and SBA have a capability as a tool for differentiating between C. polyrikoides and G. catenatum, although these species are closely similar under the light microscope fiexed with Lugol solution.

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The Cultural and Environmental Unsoundness of the Chinese Public Squatting-Type Toilet: A Case Study toward a Sustainable Excreta Treatment System

  • Chang, Jin-Soo
    • Environmental Engineering Research
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    • 제19권2호
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    • pp.131-138
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    • 2014
  • The inconvenient truth of sustainable public squat toilet culture varies among nationalities. This culture may be comfortable to the people of China, yet uncomfortable to the non-Chinese, according to the adequate environmental management in Yanbian Korean Autonomous Prefecture (YKAP), northern China. We conducted a series of field surveys and individual interviews (Chinese n = 1,000 and non-Chinese [foreign visitors] n = 100) on several aspects of the public squat toilet: structural properties, waste disposal methods, important factors, and overall satisfaction level. The significant factors in response to the public squat toilets were cleanliness, odor, toilet paper, temperature, soap, other facilities, and presence of cubicles. These factors should be policy priorities of the local government. In addition, 66.2% of Chinese and 91% of foreign visitors desired type E toilets (two full-high partition walls and a door). The results illustrate the nature of a sustainable and beautiful approach to the culturally and environmentally sound management of various types of public squat toilet in YKAP. The government needs to focus on the future-oriented and excreta treatment management of the sustainable toilet culture for residents and visitors of YKAP.