• Title/Summary/Keyword: Laboratory analysis

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Experimental setup for elemental analysis using prompt gamma rays at research reactor IBR-2

  • Hramco, C.;Turlybekuly, K.;Borzakov, S.B.;Gundorin, N.A.;Lychagin, E.V.;Nehaev, G.V.;Muzychka, A. Yu;Strelkov, A.V.;Teymurov, E.
    • Nuclear Engineering and Technology
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    • v.54 no.8
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    • pp.2999-3005
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    • 2022
  • The new experimental setup has been built at the 11b channel of the IBR-2 research reactor at FLNP, JINR, to study the elemental composition of samples by registration of prompt gamma emission during thermal neutron capture. The setup consists of a curved mirror neutron guide and a radiation-resistant HPGe high-purity germanium detector. The detector is surrounded by lead shielding to suppress the natural background gamma level. The sample is placed in a vacuum channel and surrounded by a LiF shield to suppress the gamma background generated by scattered neutrons. This work presents characteristics of the experimental setup. An example of hydrogen concentration determining in a diamond powder made by detonation synthesis is given and on its basis, the sensitivity of the setup is calculated being ~4 ㎍.

Analysis of Induction Heating Vessel using step response (STEP 응답을 이용한 유도가열조리기의 부하해석)

  • Han, G.H.;Jung, Y.C.;Roh, H.S.;Kwon, K.A.;Yang, W.J.
    • Proceedings of the KIEE Conference
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    • 1994.07a
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    • pp.319-321
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    • 1994
  • This paper describes the analysis and modeling process of vessel which is used for induction heating jar and induction healing cooker. We present how to calculate the value of modeling parameter according to the shape of the vessel and work coil, and the temperature of vessel.

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Flow blockage analysis for fuel assembly in a lead-based fast reactor

  • Wang, Chenglong;Wu, Di;Gui, Minyang;Cai, Rong;Zhu, Dahuan;Zhang, Dalin;Tian, Wenxi;Qiu, Suizheng;Su, G.H.
    • Nuclear Engineering and Technology
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    • v.53 no.10
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    • pp.3217-3228
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    • 2021
  • Flow blockage of the fuel assembly in the lead-based fast reactor (LFR) may produce critical local spots, which will result in cladding failure and threaten reactor safety. In this study, the flow blockage characteristics were analyzed with the sub-channel analysis method, and the circumferentially-varied method was employed for considering the non-uniform distribution of circumferential temperature. The developed sub-channel analysis code SACOS-PB was validated by a heat transfer experiment in a blocked 19-rod bundle cooled by lead-bismuth eutectic. The deviations between the predicted coolant temperature and experimental values are within ±5%, including small and large flow blockage scenarios. And the temperature distributions of the fuel rod could be better simulated by the circumferentially-varied method for the small blockage scenario. Based on the validated code, the analysis of blockage characteristics was conducted. It could be seen from the temperature and flow distributions that a large blockage accident is more destructive compared with a small one. The sensitivity analysis shows that the closer the blockage location is to the exit, the more dangerous the accident is. Similarly, a larger blockage length will lead to a more serious case. And a higher exit temperature will be generated resulting from a higher peak coolant temperature of the blocked region. This work could provide a reference for the future design and development of the LFR.

Differences in Colistin-resistant Acinetobacter baumannii Clinical Isolates Between Patients With and Without Prior Colistin Treatment

  • Park, Yu Jin;Hong, Duck Jin;Yoon, Eun-Jeong;Kim, Dokyun;Choi, Min Hyuk;Hong, Jun Sung;Lee, Hyukmin;Yong, Dongeun;Jeong, Seok Hoon
    • Annals of Laboratory Medicine
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    • v.38 no.6
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    • pp.545-554
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    • 2018
  • Background: The increasing morbidity and mortality rates associated with Acinetobacter baumannii are due to the emergence of drug resistance and the limited treatment options. We compared characteristics of colistin-resistant Acinetobacter baumannii (CR-AB) clinical isolates recovered from patients with and without prior colistin treatment. We assessed whether prior colistin treatment affects the resistance mechanism of CR-AB isolates, mortality rates, and clinical characteristics. Additionally, a proper method for identifying CR-AB was determined. Methods: We collected 36 non-duplicate CR-AB clinical isolates resistant to colistin. Antimicrobial susceptibility testing, Sanger sequencing analysis, molecular typing, lipid A structure analysis, and in vitro synergy testing were performed. Eleven colistin-susceptible AB isolates were used as controls. Results: Despite no differences in clinical characteristics between patients with and without prior colistin treatment, resistance-causing genetic mutations were more frequent in isolates from colistin-treated patients. Distinct mutations were overlooked via the Sanger sequencing method, perhaps because of a masking effect by the colistin-susceptible AB subpopulation of CR-AB isolates lacking genetic mutations. However, modified lipid A analysis revealed colistin resistance peaks, despite the population heterogeneity, and peak levels were significantly different between the groups. Conclusions: Although prior colistin use did not induce clinical or susceptibility differences, we demonstrated that identification of CR-AB by sequencing is insufficient. We propose that population heterogeneity has a masking effect, especially in colistin non-treated patients; therefore, accurate testing methods reflecting physiological alterations of the bacteria, such as phosphoethanolamine-modified lipid A identification by matrix-assisted laser desorption ionization-time of flight, should be employed.

Molecular characterization of chicken anemia virus in Guangxi Province, southern China, from 2018 to 2020

  • Zhang, Minxiu;Deng, Xianwen;Xie, Zhixun;Zhang, Yanfang;Xie, Zhiqin;Xie, Liji;Luo, Sisi;Fan, Qing;Zeng, Tingting;Huang, Jiaoling;Wang, Sheng
    • Journal of Veterinary Science
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    • v.23 no.5
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    • pp.63.1-63.14
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    • 2022
  • Background: Chicken anemia virus (CAV) causes chicken infectious anemia, which results in immunosuppression; the virus has spread widely in chicken flocks in China. Objectives: The aim of this study was to understand recent CAV genetic evolution in chicken flocks in Guangxi Province, southern China. Methods: In total, 350 liver samples were collected from eight commercial broiler chicken farms in Guangxi Province in southern China from 2018 to 2020. CAV was detected by conventional PCR, and twenty CAV complete genomes were amplified and used for the phylogenetic analysis and recombination analysis. Results: The overall CAV-positive rate was 17.1%. The genetic analysis revealed that 84 CAVs were distributed in groups A, B, C (subgroups C1-C3) and D. In total, 30 of 47 Chinese CAV sequences from 2005-2020 belong to subgroup C3, including 15 CAVs from this study. There were some specific mutation sites among the intergenotypes in the VP1 protein. The amino acids at position 394Q in the VP1 protein of 20 CAV strains were consistent with the characteristics of a highly pathogenic strain. GX1904B was a putative recombinant. Conclusions: Subgroup C3 was the dominant genotype in Guangxi Province from 2018-2020. The 20 CAV strains in this study might be virulent according to the amino acid residue analysis. These data help improve our understanding of the epidemiological trends of CAV in southern China.

Recent Development of Laboratory-made Solid-phase Microextraction Fibers on the Application of Food Safety Analysis

  • Zeng, Jingbin;Chen, Jinmei;Chen, Wenfeng;Huang, Xiaoli;Chen, Liangbi;Chen, Xi
    • Food Science and Biotechnology
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    • v.18 no.3
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    • pp.579-585
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    • 2009
  • Solid-phase microextraction (SPME) has gained widespread acceptance in sample pretreatment due to its solvent-free and easy-to-operate properties. SPME fibers are considered as a key part of SPME technique, since it primarily determines the extraction performance of the method including sensitivity, selectivity, and reproducibility. Generally speaking, target analyte with different chemical property requires fiber coating that has the best affinity towards it. Due to the lack of varieties of commercial fibers available currently, considerable efforts have been recently made to develop tailor-made fibers to fulfill increasing demands of different analysis. This paper concisely classify some SPME fiber preparation approaches such as sol-gel technology, physical deposition, molecularly imprinted technique, and their respective application in food safety analysis.

INTERLAYER COUPLING AND MAGNETOOPTICS IN MULTILAYERS

  • Lu, M.;Bie, Q.S.;Xu, Y.B.;Zhai, H.R.;Zhou, S.M.;Chen, Liangyao;Jin, Q.Y.
    • Journal of the Korean Magnetics Society
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    • v.5 no.5
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    • pp.561-566
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    • 1995
  • Additional magnetooptical Kerr effect (AMOKE) was observed in several multilayer structures. For Fe/Pd and Co/Cu Multilayers, AMOKE enhanced the Kerr rotation in short wavelength side, while for Fe/Ag and FeSi/Cu multilayer systems the Kerr rotation enhancement appeared in long wavelength side. A number of ferromagnetic/nonmagnetic/ferromagnetic(FM/NM/FM) sandwiches showed that the AMOKE led to oscillations of Kerr rotation and Kerr ellipticity in certain wavelength range with changing NM layer thickness similar to the oscillatory interlayer coupling. The oscillation of effective optical constants related to the MOKE oscillation was observed for the first time. The mechanisms of the AMOKE were discussed.

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VIBRATION SIGNAL ANALYSIS OF MAIN COOLANT PUMP FLYWHEEL BASED ON HILBERT-HUANG TRANSFORM

  • LIU, MEIRU;XIA, HONG;SUN, LIN;LI, BIN;YANG, YANG
    • Nuclear Engineering and Technology
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    • v.47 no.2
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    • pp.219-225
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    • 2015
  • In this paper, a three-dimensional model for the dynamic analysis of a flywheel based on the finite element method is presented. The static structure analysis for the model provides stress and strain distribution cloud charts. The modal analysis provides the basis of dynamic analysis due to its ability to obtain the natural frequencies and the vibration-made vectors of the first 10 orders. The results show the main faults are attrition and cracks, while also indicating the locations and patterns of faults. The harmonic response simulation was performed to gain the vibration response of the flywheel under operation. In this paper, we present a Hilbert-Huang transform (HHT) algorithm for flywheel vibration analysis. The simulation indicated that the proposed flywheel vibration signal analysis method performs well, which means that the method can lay the foundation for the detection and diagnosis in a reactor main coolant pump.

Uncertainty Evaluation of the Analysis of Methamphetamine and Amphetamine in Human Hair by GC/MS (GC/MS를 이용한 모발 중 메스암페타민 및 암페타민 분석의 측정불확도 평가)

  • Kim, Jin-Young;Kim, Jong-Sang;Kim, Min-Kyoung;Lee, Jae-Il;Suh, Yong-Jun;In, Moon-kyo
    • YAKHAK HOEJI
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    • v.51 no.3
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    • pp.206-213
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    • 2007
  • Recently estimating the uncertainty of an analytical result has become an essential part of quantitative analysis. This study describes the uncertainty of the measurement for the determination of methamphetamine and its major metabolite amphetamine in human hair, The method consists of washing, drying, weighing, incubation and extraction with methanolic HCI solution, clean-up, trifluoroacetyl derivatization, and qualification/quantification of residues by gas chromatography/mass spectrometry (GC/MS). Traceability of measurement was established through traceable standards and calibrated volumetric equipment and measuring instruments. Measurement uncertainty associated with each analyte in real samples was estimated using quality control (QC) data. The main source of combined standard uncertainty comprised two components, which are uncertainties associated with calibration linearity and variations in QC, while those associated with preparation of analytical standards and sample weighing were not so important considering the degree of contribution. Relative combined standard uncertainties associated with the described method ranged for individual analytes from 4.99 to5.03%.