• Title/Summary/Keyword: Laboratory Facilities

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Bypass Fat Production Using Acid Oil, Its Effect on In Vitro Rumen Fermentation and Effect of Its Feeding on In Sacco DM Disappearance in Sheep

  • Garg, M.R.
    • Asian-Australasian Journal of Animal Sciences
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    • v.10 no.6
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    • pp.571-574
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    • 1997
  • Attempts were made in the laboratory to produce bypass fat using acid oil by precipitation and fusion methods. The degree of saponification by both of these methods was above 80 percent. Where heating facilities are not available, precipitation method could be used, otherwise, fusion method of bypass fat production is found to be more convenient, especially for commercial scale operations as handling of large volume of solutions is eliminated. Bypass fat thus produced was tested in vitro for rumen fermentation. Incorporation of acid oil in the incubation medium reduced TVFA conc. from 127.06 to 124.09 mM/l SRL and increased ammonia-N levels from 210.50 to 223 mg/l SRL indicating that the microbial activity was affected on incorporation of acid oil in the incubation medium. However, incorporation of bypass fat in the incubation medium did not significantly affect TVFA conc. as well as ammonia-N levels. In another experiment, nine rumen fistulated sheep in three groups of three each were fed bypass fat at two different levels. Dry matter disappearance in 24 h from the nylon bags suspended in the rumen of animals under different groups was found to be $47.74{\pm}1.10$, $47.55{\pm}0.21$ and $50.74{\pm}1.11$ in group I (control), group II (fed bypass fat 50 g/day) and group III (fed bypass fat 100 g/day), respectively. These studies indicated that it is possible to produce bypass fat from acid oils, a by-product of oil refining process, and its feeding did not affect rumen fermentation.

Profile of Skin Biopsies and Patterns of Skin Cancer in a Tertiary Care Center of Western Nepal

  • Kumar, Ajay;Shrestha, Prashanna Raj;Pun, Jenny;Thapa, Pratichya;Manandhar, Merina;Sathian, Brijesh
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.8
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    • pp.3403-3406
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    • 2015
  • Background: Skin biopsy is the method to assist clinicians to make definite dermatological diagnosis which further helps in holistic management. Skin cancers are relatively rare clinical diagnosis in developing countries like Nepal, but the prevalence is on rise. Objectives: To investigate the profile of skin biopsies and frequencies and pattern of skin cancers in a tertiary care centre of Western Nepal. Materials and Methods: The materials consisted of 434 biopsies (1.37%) out of 31,450 OPD visits performed in the Department of Dermatology, Manipal Teaching Hospital, Pokhara, Nepal, during the period of Dec 2011-Nov 2014. Data were collected and analyzed using SPSS-16 with reference to incidence, age, sex, race and clinical and histopathological features. Results: The commonest disorders observed in biopsies were papulosquamous lesions, skin tuberculosis of different types, benign skin tumors, leprosy, collagen and fungal diseases. Viral diseases were rarely seen, probably due to straight forward clinical diagnosis. Dermatological malignancies accounted for 55/434 (12.67%) of biopsies. Skin disorders in general were commoner in females 280/434 (64%), including malignancies 32/55(58.2%). Mean age of patients with skin cancer was 54.5 years. Facilities for proper laboratory investigation of dermatological disorders will improve the quality of life. Conclusions: The most prevalent lesion in skin biopsies was papulosquamous disorders followed by skin tuberculosis of different types. Dermatological malignancy constituted 55/434 (12.67%) cases. The prevalence of skin malignancy is on rise in Nepalese society probably due to increase in life expectancy and better diagnostic services.

Sustainable Utilization and Management Scheme in Wangdol-cho Surrounding Sea Area (동해 왕돌초 어장의 지속적 이용 및 관리 방안)

  • Lee, Kwang-Nam;Myoung, Jung-Goo
    • Ocean and Polar Research
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    • v.25 no.3
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    • pp.331-345
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    • 2003
  • The fishing ground surrounding Wangdol-cho is not only overexploited by the littering of dilapidated fishing net and equipment, but also by fishermen's overfishing, surpassing optimum fisheries resources. In addition, increasing fishing efforts (number of fishing vessel and fishing net, etc) contribute to the deterioration of fishing ground, and it is urgently required that schemes to tackle the problems should be taken. To effectively address the problems as such, this paper aims to propose sustainable utilization and management scheme of fishing ground through classification of fishing ground surrounding Wangdol-cho as one area which is less than 50m deep, measuring $13.66km^2$ and the other, permission fishing area of Gill Net fishery, measuring $347.23km^2$. The analysis shows that, for the water area less than 50m deep, implementation from a short-term perspective includes autonomous management fishery by gill net and trap fishery. For the permission fishing area of Gill Net fishery, implementation includes limit on fishing period, real name system of fishing equipment and limit on fishing equipment. Implementation from a medium and long-term perspective includes limit on scuba diving, designation of underwater sightseeing zone, sea farming, facilities of surveillance, adoption of approval system for the permission fishing area of Gill Net fishery and introduction of report system for fishing.

Current Management Status of Mercury Emissions from Coal Combustion Facilities: International Regulations, Sampling Methods, and Control Technologies

  • Lee, Sung-Jun;Pudasainee, Deepak;Seo, Yong-Chil
    • Journal of Korean Society for Atmospheric Environment
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    • v.24 no.E1
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    • pp.1-11
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    • 2008
  • Mercury (Hg), which is mainly emitted from coal-fired power plants, remains one of the most toxic compounds to both humans and ecosystems. Hg pollution is not a local or regional issue, but a global issue. Hg compounds emitted from anthropogenic sources such as coal-fired power plants, incinerators, and boilers, can be transported over long distances. Since the last decade, many European countries, Canada, and especially the United States, have focused on technology to control Hg emissions. Korea has also recently showed an interest in managing Hg pollution from various combustion sources. Previous studies indicate that coal-fired power plants are one of the major sources of Hg in Korea. However, lack of Hg emission data and feasible emission controls have been major obstacles in Hg study. In order to achieve effective Hg control, understanding the characteristics of current Hg sampling methods and control technologies is essential. There is no one proven technology that fits all Hg emission sources, because Hg emission and control efficiency depend on fuel type, configuration of air pollution control devices, flue gas composition, among others. Therefore, a broad knowledge of Hg sampling and control technologies is necessary to select the most suitable method for each Hg-emitting source. In this paper, various Hg sampling methods, including wet chemistry, dry sorbents trap, field, and laboratory demonstrated control technologies, and international regulations, are introduced, with a focus on coal-fired power plants.

Rapid Detection of Lily mottle virus and Arabis mosaic virus Infecting Lily (Lilium spp.) Using Reverse Transcription Loop-Mediated Isothermal Amplification

  • Zhang, Yubao;Wang, Yajun;Xie, Zhongkui;Wang, Ruoyu;Guo, Zhihong;He, Yuhui
    • The Plant Pathology Journal
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    • v.36 no.2
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    • pp.170-178
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    • 2020
  • The Lily mottle virus (LMoV) impedes the growth and quality of lily crops in Lanzhou, China. Recently Arabis mosaic virus (ArMV) has been detected in LMoV-infected plants in this region, causing plant stunting as well as severe foliar symptoms, and likely posing a threat to lily production. Consequently, there is a need to develop simple, sensitive, and reliable detection methods for these two viruses to prevent them from spreading. Reverse transcription (RT) loop-mediated isothermal amplification (LAMP) assays have been developed to detect LMoV and ArMV using two primer pairs that match six conserved sequences of LMoV and ArMV coat proteins, respectively. RT-LAMP assay results were visually assessed in reaction tubes using green fluorescence and gel electrophoresis. Our assays successfully detected both LMoV and ArMV in lily plants without the occurrence of viral cross-reactivity from other lily viruses. Optimal conditions for LAMP reactions were 65℃ and 60℃ for 60 min for LMoV and ArMV, respectively. Detection sensitivity for both RT-LAMP assays was a hundredfold greater than that of our comparative RT-polymerase chain reaction assays. We have also found this relatively rapid, target specific and sensitive method can also be used for samples collected in the field and may be especially useful in regions with limited or no laboratory facilities.

Beyond SARS-CoV-2: Lessons That African Governments Can Apply in Preparation for Possible Future Epidemics

  • Oboh, Mary Aigbiremo;Omoleke, Semeeh Akinwale;Imafidon, Christian Eseigbe;Ajibola, Olumide;Oriero, Eniyou Cheryll;Amambua-Ngwa, Alfred
    • Journal of Preventive Medicine and Public Health
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    • v.53 no.5
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    • pp.307-310
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    • 2020
  • Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has placed unprecedented pressure on healthcare systems, even in advanced economies. While the number of cases of SARS-CoV-2 in Africa compared to other continents has so far been low, there are concerns about under-reporting, inadequate diagnostic tools, and insufficient treatment facilities. Moreover, proactiveness on the part of African governments has been under scrutiny. For instance, issues have emerged regarding the responsiveness of African countries in closing international borders to limit trans-continental transmission of the virus. Overdependence on imported products and outsourced services could have contributed to African governments' hesitation to shut down international air and seaports. In this era of emerging and re-emerging pathogens, we recommend that African nations should consider self-sufficiency in the health sector as an urgent priority, as this will not be the last outbreak to occur. In addition to the Regional Disease Surveillance Systems Enhancement fund (US$600 million) provided by the World Bank for strengthening health systems and disease surveillance, each country should further establish an epidemic emergency fund for epidemic preparedness and response. We also recommend that epidemic surveillance units should create a secure database of previous and ongoing pandemics in terms of aetiology, spread, and treatment, as well as financial management records. Strategic collection and analysis of data should also be a central focus of these units to facilitate studies of disease trends and to estimate the scale of requirements in preparation and response to any future pandemic or epidemic.

Study on the Water Quality Control of Water Supply the Reside: Effects of Chlorinations (상수도의 수질관리와 타소소독의 잔류효과에 관한 연구)

  • 유귀현
    • Journal of environmental and Sanitary engineering
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    • v.6 no.2
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    • pp.33-47
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    • 1991
  • 1. Water supply treatment plants personnel could not test the water quality control, because most of them rely on the provincial health laboratories about water quality rely rant test. However, in future, plants have to been provided the facilities and equipment of water quality laboratory. 2. Chlorination methods are 89.5% of liquid chlorine and 10.5% of solid chlorine, and the cost per 1 $\m^{3}$ of chlorination is about 1.30 won which chlorination cost is very cheap as 1/142 of drinking water production cost. Input method of chlorine is 35% of automatic method, 56% of semi-automatic, and 9% of other methods, and this is no problem 3. Residual effects of chlorination, in the case of distilled water as a standardized material and 0.2 ppm of seperated residual chlorine, were continued 32 hours in $0^{\circ}C$, and 25 hour in $20^{\circ}C$, of water temperature and in the case of 0.4 ppm of seperated residual chlorine were continued 47 hours in $0^{\circ}C$ and 23 hours in $20^{\circ}C$. 4. In the case of 4 ppm of seperated residual chlorine, residual effects were continued 23 hours in $5^{\circ}C$, 90 hours in $10^{\circ}C$, 78 hours in $15^{\circ}C$, and 60 hours in $20^{\circ}C$ : by the temper; lure of water, continuing residual effects of chlorination are different, so we have to car for the warm season chlorination in the hider temperature. 5. Chlorination effects of drinking waters in the case of 0.4 ppm of seperated residual chlorine were continued 237 hours in $22^{\circ}C$ water : and in the case of rechlorination as 4 ppm of residual chlorine, continued 71 hours in $22^{\circ}C$ water.

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Seismic Retrofit Effect for Column of Subway Tunnel Reinforced by FRP-Ductile Material Layered Composites (FRP-연성재 적층복합체로 보강된 도시철도 개착식 터널 기둥의 내진보강효과)

  • Kim, Doo-Kie;Go, Sung-Hyuk;Kim, Jin-Yeong
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.14 no.6
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    • pp.85-92
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    • 2010
  • Recently the earthquake occurrences in Korea are likely to increase. Therefore, the facilities such as bridges and tunnels under the unexpected earthquakes need to be enhanced. Especially most of the subways previously built before 1988 have not been seismically designed, so their seismic safety requirements are required to be inspected and/or reinforced. In this study, the seismic reinforcement using FRP-ductile material layered composites was proposed to reinforce for the subway columns. Material properties of FRP-ductile material layered composites were calculated by laboratory tests considering the laminated conditions of the composites. Numerical simulations were performed using the experimental results of the specimens and the calculated properties of the composites. Seismic performance varied according to the types of composites: ductile material, number of layers, fiber orientations.

Study on the Influence of Stray current Between Sacrificial Anode Cathodic Protection and Impressed Current Cathodic Protection in Marine Environment

  • Jeong, Jin-A;Kim, Ki-Joon
    • Corrosion Science and Technology
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    • v.11 no.3
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    • pp.77-81
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    • 2012
  • Cathodic protection(CP) is widely used as a means of protecting corrosion for not only marine structures like ship hulls and offshore drilling facilities, but also underground structures like buried pipelines and oil storage tanks. The principle of CP is that the anodic dissolution of metal can be protected by supplying electrons to the cathode metal. When unprotected structures are nearby to CP systems, interference problems between unprotected and protected structures may be happened. The stray current interference can accelerate the corrosion of nearby structures. So far many efforts have been made to reduce the interference in the electric railway systems adjacent to the underground metal structures like buried pipelines and gas/oil tanks. During recent few decades the protection technologies against stray current induced corrosion have been significantly improved and a number of techniques have been developed. However, there is very limited information an marine environments. Some complex harbor structures are protected by two cathodic protection systems, i.e. sacrificial anode cathodic protection(SACP) and impressed current cathodic protection(ICCP). In this case, when the protection current from sacrificial anodes returns to the cathode through electrolyte, it passes through nearby other low resistance metal structures. In many cases the stray current of ICCP systems influences the function of SACP. In this study, the risk of stray current from the SACP system to adjacent reinforced concrete structures has been verified through laboratory experiments. Concrete and steel pile structures modeled a part of bridge have been investigated in terms of CP potential and current between the two. The variation of stray current according to the magnitude of ICCP/SACP has been studied to mitigate it and to suggest the proper protection criteria.

A Study on Optimum Conditions Derivation on Thermal Hydrolysis of Food Wastewater and the Applicability of the Thermal Solubilization in Biological Denitrification Process (음폐수의 열가수분해 최적조건 도출과 생물학적 탈질공정에서 열가용화액의 적용 가능성에 관한 연구)

  • Lee, Ki Hee;You, Hee Gu;Joo, Hyun Jong
    • Journal of Korean Society on Water Environment
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    • v.31 no.2
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    • pp.151-158
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    • 2015
  • The aim of this research is to derive an optimum operating condition for the thermal solubilization equipment that is employed to increase concentration of soluble organic materials and to assess whether it would be possible to use the waste sludge generated by thermal solubilization reaction as an external carbon source in biological denitrification process. For the purpose, we have constituted a laboratory-size thermal solubilization equipment and have assessed thermal hydrolysis efficiency based on various reaction temperature and reaction time. We have also derived SDNR using the waste sludge generated by thermal solubilization reaction through a batch experiment. As a result of research, the highest thermal hydrolysis efficiency of about 42.8% was achieved at $190^{\circ}C$ of reaction temperature and at 90 minutes of reaction time. And when SDNR was derived using the waste sludge, the value obtained was $0.080{\sim}0.094\;g\;NO_3{^-}-N/g\;MLVSS{\cdot}day$, showing SDNR that is higher than that obtained by the results of existing researches that used common wastewater as an external carbon source. Accordingly, in view of the fact that food wastes vary quite a bit in characteristics based on the area they are generated from and seasonal change, it seems that a flexible operation of thermal solubilization equipment is required through on-going monitoring of food wastes that are imported to food wastes recycling facilities.