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The Effects of Alkaloid Fraction of Korean Ginseng on the Radiation-Induced DNA Strand Breaks (방사선 조사에 의한 DNA Double Strand Breaks의 생성 및 회복에 미치는 인삼 알칼로이드 분획의 효과)

  • Cho Chul Koo;Kim Tae Hwan;Yoo Seong Yul;Koh Kyoung Hwan;Kim Mi Sook;Kim Jeong Hee;Kim Seong Ho;Yoon Hyung Keun;Ji Young Hoon
    • Radiation Oncology Journal
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    • v.13 no.2
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    • pp.113-120
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    • 1995
  • Purpose : To investigate the effect of alkaloid fraction from Korean ginseng on radiation-induced DNA double strand breaks (dsb) formation and repair in murine lymphocytes Materials and Methods : We used the neutral filter elution technique to assay $^{60}Co\;{\gamma}$ ray-induced DNA double strand breaks formation and repair in C57BL/6 mouse spleen lymphocytes for evaluating the dose-response relationship in the presence of alkaloid fraction as a radioprotective agent. The lymphocytes were stimulated with Phytohemagglutinin (PHA, 2 u g/ml) to label $^3[H]-thymidine.$ Isotope-labelled lymphocytes in suspension were exposed to 100 Gy at $0^{\cdot}C$ in the alkaloid fraction-treated group and elution procedure was performed at PH 9.6. The extents of formation of radiation-induced DNA double strand breaks and repair were compared respectively via strand scission factor (SSF) and relative strand scission factor (RSSF). Results: Alkaloid fraction reduced the formation of double strand breaks with dose modification factor of 2 15, compared to control group Rejoining of DNA dsb appeared to take place via two components. The first fast component was completed within 20.4 minutes, but the second slow component was not completed until 220.2 minutes after irradiation. About $30\%$ of dsb formed by irradiation was ultimately unrejoined despite the administration of alkaloid fraction. The administration of alkaloid fraction had a great effect on the second slow component of repair; the half-time of fast component repair was not changed, but that of slow component was 621.8 minutes. Conclusion: Neutral filter elution assay Proved to be a very effective method to quantitate the extents of DNA dsb formation and its repair. By using this technique, we were able to evaluate the efficiency of alkaloid fraction from Korean ginseng as a valuable radioprotector. Alkaloid fraction can be used prophylactically to prevent or ameliorate the severe radiation damages in workers and neighbors around the atomic power plants. For more refined study, however, more advanced purification of alkaloid fraction wil be needed in the near future.

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Quality Comparison of Luncheon Meats (런천미트 통조림의 품질비교)

  • Park, H.I.;Yang, S.Y.;Chung, M.S.;Lee, M.
    • Korean Journal of Food Science and Technology
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    • v.24 no.5
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    • pp.492-496
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    • 1992
  • In order to compare the quality of canned pork products which are called collectively as luncheon meat, residual nitrite, sodium, collagen, total heme pigments and chemical composition were analyzed in 12 products of 8 companies from 4 countries. Also, the proteins of products were compared with that of pork by SDS-PAGE analysis. The level of residual nitrite was low in all the products and sodium levels were similar except in one or two products. As for collagen and total heme pigments content, among imported products luncheon meats were different from chopped meat products while domestic products were similar regardless of label distinction. Collagen contents of domestic products were similar to those of imports but total heme pigments contents were much higher Densitometer scans of gel electrophoretograms of chopped meat were more similar to that of pork than those of luncheon meat. In terms of chemical composition, luncheon meat had more carbohydrate regardless of whether they are domestics or imports. The quality of domestic luncheon meat appears to be the composite of those of imported luncheon meat and chopped meat. Accordingly, the quality standard for luncheon meat as a cheap product should be established in Korea to enable the domestic products to have a competitive power in price.

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An study on purchasing activity of Private Brand clothing in large discount store (소비자들의 대형마트 PB의류 구매행동에 관한 연구)

  • Kim, Young-Hwan;Kim, Pan-Jin
    • Journal of Distribution Science
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    • v.6 no.1
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    • pp.117-128
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    • 2008
  • The purpose of the research is to furnish basic data for efficient and effective marketing by examining the correlation how a certain element effects on customer's selection behavior, when they choose private label branded clothes at the store of giant discount store like E-.mart, Home-plus, Lotte-mart and 2001 outlet. We come to the following conclusions through such procedures; What we found on this study is that main factors of decision related purchasing garment of private brand in giant discount store is based on the sales price, quality of products and needs of customer's using. Further to this, in terms of choice activity in private brand clothes, we realized the price value is depend on the resonable price with the items because nowadays most customers can be compare to other discount store around their shopping place including trend of garment and quality of products. The order of the general inclination in selection behavior of private branded garment in giant discount store is as follows; Sales price > style & trend > quality of product and substantial convenience > display and prompt buying. Judging from the conclusion of the research we noted that customer's satisfaction comes from their target price to supply substantial needs and in terms of styling of clothes should be considered current trendy compared to national brand as well.

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Measuring in vivo Rate of Bone Collagen Synthesis in Growing Rats (성장기 흰쥐의 골조직 Collagen 생성속도 측정)

  • 김유경
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.8
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    • pp.1390-1393
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    • 2003
  • Measuring in vivo rate of bone collagen synthesis has so far been technically difficult and often subject to quite large errors. In the present study, bone collagen synthesis rate was measured using a precursor-product method, based on the exchange of $^2$$H_2O$ into amino acids. Mass isotopomer abundance in hydroxyproline from bone collagen was analyzed by gas chromatography/mass spectrometry. The $^2$$H_2O$ labeling protocol consisted of an initial intraperitoneal injection of 99.9% $^2$$H_2O$, to achieve approximately 2.5% body water enrichment followed by administration of 4% $^2$$H_2O$ in drinking water for 9 weeks. Body $^2$$H_2O$ enrichments were stable at 2.7 ∼ 3.0% over labeling Period. In growing rats, the fractional synthesis rate ( $k_{s}$) of bone collagen was 0.066 $\pm$ 0.049 w $k^{-1}$ . The unique features of stable $^2$$H_2O$ pools and label incorporation allowed the precursor-product approach to be used for measuring bone collagen synthesis rate..

Consumers의 Awareness and Utilization of Food Labels (식품표시에 대한 소비자의 인식 및 이용 실태)

  • 김향숙;백수진;이경애
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.4
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    • pp.948-953
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    • 1999
  • The objective of this study was to contribute to the enhancement of food labeling and the development of food labels which are more informative to consumers. The questionnaires had been collected from adult female consumers living in Seoul, Kyonggi, Choongnam and Chungbuk province. Out of 950 answers collected 792 were analyzed. Ninety two percent of respondents were aware of food labels, however only 67.6% of them knew about the fact that food labeling was regulated by the law. Respondents recognized the major purpose of food labeling as informing and protecting the consumer. Most of the respondents answered that they confirmed the contents of food labels at the time of purchase especially with regard to safety aspects, such as expiration on data and manufacture date. Eighty four percent of the respon dents showed confidence in the contents of food labels.The rests answered they were hot confident because they experienced directly, or indirectly discord of food contents in fact and in label.

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Detection of Undeclared Betamethasone Derivatives in Cosmetic Products Labeled to Contain Zinc Pyrithione as the Active Ingredient (아연피리치온을 유효성분으로 표기한 화장품류에서 미표기 성분인 베타메타손 유도체의 검출)

  • Lee, Jeong-Pyo;Park, Sung-Hwan;Yang, Seong-Jun;Kim, Sun-Mi;Son, Kyung-Hun;Yun, Mi-Ok;Choi, Sang-Sook
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.35 no.1
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    • pp.11-17
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    • 2009
  • Betamethasone propionate, an anti-inflammatory glucocorticosteroid, was detected in cosmetics with no indication on the label of this compound as an ingredient. The product was formulated as a topical spray or shampoo and labeled to contain zinc pyrithione as the active ingredient. A thin-layer chromatographic analysis was carried out on silica gel plates to provide a first indication about the presence of a compound with steroid structure and reactivity; then high-performance liquid chromatography (HPLC) separation allowed the identification of the corticosteroid agent and its quantification. To identify the corticosteroid agent from these commercial samples we collected the fractions suspected to have ketol steroids by prep HPLC and identified the compound as betamethasone propionate by NMR and MS spectrometry. Then we synthesized the standard for the betamethasone 17-propionate and 21-propionate and quantitate the corticosteroids from the sample by HPLC with that standards. By this method we identified the corticosteroid compounds from some commercial cosmetics such as zinc pyrithione sprays. The finding of betamethasone propionate in the products was shown by comparison to an authenticated standard of betamethasone propionate by retention time on reverse-phase HPLC. Two of the tested products contained betamethasone propionate at the levels of 0.005 ${\sim}$ 0.02% and the others were free of betamethasone propionate.

Visitors' Evaluation of Information and Interpretive Media in Dadohaehaesang National Park, Korea (다도해해상국립공원 탐방객의 홍보 및 환경해설 매체 이용평가)

  • Cho, Woo;Kim, Dong-Pil;Choi, Song-Hyun;Hong, Suk-Hwan
    • Korean Journal of Environment and Ecology
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    • v.27 no.5
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    • pp.642-649
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    • 2013
  • This study evaluated the efficacy of information and self-interpretive media that provide information on park environment using self-administered visitor survey to the Dadohaehaesang National Park, Korea. Excluding missing and unreliable responses, 205 valid responses were used for the analysis. Socioeconomic status and visiting behavior of the visitors to the Dadohaehaesang National Park were similar to those to other Korean national parks. Results showed that, of the self-interpretive media, 'Information board of park use and resources' were most frequently used (87.7%), followed by 'Interpretive label of woody plant,' and 'Bulletin boards for information and enlightenment.' 'Guided interpretation' was used less than 40% of the visitors. Visitors also highly rated the importance of the media (higher than 4.0 on average out of 5 point Liker scale question). The average performance rate was 3.82, suggesting that visitors were satisfied on the self-interpretive media. Visitors responded that 'Information board of park use and resources' and 'Bulletin boards for information and enlightenment' were not useful and, therefore, should be amended and managed to improve the self-interpretability of the media.

The Ability to Identify the Imported Foods among Housewives in Cheongju Area

  • Kim Kinam;Park Eunjin;Cho Jinsuk
    • Journal of Community Nutrition
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    • v.7 no.2
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    • pp.85-92
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    • 2005
  • The purpose of the study was to get some information about the educational program for consumers in the community. For this, the author investigated the ability to identify the imported foods and the educational contents to be taught. The subjects for this study were 183 housewives living in Cheongju city, and the research was conducted from March 1 to March 15, 2003. A survey questionnaire was distributed, asking general matters regarding the subject, attitude when purchasing foods, the ability to identify the imported foods, educational contents to be taught, and the identification ability between the domestic and the imported foods. The data was analyzed for percentage, mean, standard deviations using SAS program, and was also examined with Chi-square or ANOVA. $92.3\%$ of housewives checked the label to distinguish between the domestic food and the imported one, among which $99.5\%$ preferred to buy the domestic brand. For major reasons of this preference, $46.3\%$ of them reported that the imported foods had many harmful substances. $66.1\%$ of the subjects, however, replied that they did not have the ability to discern the differences between the two. The identification information was received from 'TV or radio program', which $61.7\%$ of the subjects reported as such. $61.5\%$ of the subjects were inclined to receive education about the imported foods. For the educational contents, $75.4\%$ wanted to learn 'the method to identify the imported foods'. According to the identification test on the imported foods, they got 13.6 points on the average out of 40 points, which was quite low. The highest correct answer was for pteridium aquilinum ($63.7\%$), sesame ($49.2\%$), and yellow croaker ($45.6\%$), while the highest incorrect answer was for red pepper powder ($40.4\%$), chestnut ($40.6\%$), and dried pepper ($32.2\%$). The results suggested that most of the respondents had negative attitudes towards the imported agricultural products, but their identification ability was quite poor. Therefore, it is necessary to provide education and publicity work in relation to the identification methods for the imported foods so that consumers may have less risk from the imported agricultural products.

MCBP Neural Netwoek for Effcient Recognition of Tire Claddification Code (타이어 분류 코드의 효율적 인식을 위한 MCBP망)

  • Koo, Gun-Seo;O, Hae-Seok
    • The Transactions of the Korea Information Processing Society
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    • v.4 no.2
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    • pp.465-482
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    • 1997
  • In this paper, we have studied on cinstructing code-recognition shstem by neural network according to a image process taking the DOT classification code stamped on tire surface.It happened to a few problems that characters distorted in edge by diffused reflection and two adjacent characters take the same label,even very sen- sitive to illumination ofr recognition the stamped them on tire.Thus,this paper would propose the algorithm for tire code under being cinscious of these properties and prove the algorithm drrciency with a simulation.Also,we have suggerted the MCBP network composing of multi-linked recognizers of dffcient identify the DOT code being tire classification code.The MCBP network extracts the projection balue for classifying each character's rdgion after taking out the prjection of each chracter's region on X,Y axis,processes each chracters by taking 7$\times$8 normalization.We have improved error rate 3% through the MCBP network and post-process comparing the DOT code Database. This approach has a accomplished that learming time get's improvenent at 60% and recognition rate has become to 95% from 90% than BckPropagation with including post- processing it has attained greate rates of entire of tire recoggnition at 98%.

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Generating Rank-Comparison Decision Rules with Variable Number of Genes for Cancer Classification (순위 비교를 기반으로 하는 다양한 유전자 개수로 이루어진 암 분류 결정 규칙의 생성)

  • Yoon, Young-Mi;Bien, Sang-Jay;Park, Sang-Hyun
    • The KIPS Transactions:PartD
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    • v.15D no.6
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    • pp.767-776
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    • 2008
  • Microarray technology is extensively being used in experimental molecular biology field. Microarray experiments generate quantitative expression measurements for thousands of genes simultaneously, which is useful for the phenotype classification of many diseases. One of the two major problems in microarray data classification is that the number of genes exceeds the number of tissue samples. The other problem is that current methods generate classifiers that are accurate but difficult to interpret. Our paper addresses these two problems. We performed a direct integration of individual microarrays with same biological objectives by transforming an expression value into a rank value within a sample and generated rank-comparison decision rules with variable number of genes for cancer classification. Our classifier is an ensemble method which has k top scoring decision rules. Each rule contains a number of genes, a relationship among involved genes, and a class label. Current classifiers which are also ensemble methods consist of k top scoring decision rules. However these classifiers fix the number of genes in each rule as a pair or a triple. In this paper we generalized the number of genes involved in each rule. The number of genes in each rule is in the range of 2 to N respectively. Generalizing the number of genes increases the robustness and the reliability of the classifier for the class prediction of an independent sample. Also our classifier is readily interpretable, accurate with small number of genes, and shed a possibility of the use in a clinical setting.