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Immunological benefits by ginseng through reciprocal regulation of Th17 and Treg cells during cyclosporine-induced immunosuppression

  • Heo, Seong Beom;Lim, Sun Woo;Jhun, Joo Yeon;Cho, Mi La;Chung, Byung Ha;Yang, Chul Woo
    • Journal of Ginseng Research
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    • v.40 no.1
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    • pp.18-27
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    • 2016
  • Background: It is not clear whether ginseng affects cyclosporine A (CsA)-induced desirable immunosuppressive action. In this study, we evaluated the immunological influence of combined treatment of ginseng with CsA. Methods: Using CD4+ T cells from mouse spleens stimulated with the T cell receptor (TCR) or allogeneic antigen-presenting cells (APCs), we examined the differentiation of naïve T cells into T helper 1 (Th1), Th2, Th17, and regulatory T cells (Tregs), and their cytokine production during treatment by Korean Red Ginseng extract (KRGE) and/or CsA. The influence of KRGE on the allogeneic T cell response was evaluated by mixed lymphocyte reaction (MLR). We also evaluated whether signal transducer and activator of transcription 3 (STAT3) and STAT5 are implicated in this regulation. Results: Under TCR stimulation, KRGE treatment did not affect the population of CD4+interferon gamma ($IFN{\gamma}$)+ and CD4+interleukin (IL)-4+ cells and their cytokine production compared with CsA alone. Under the Th17-polarizing condition, KRGE significantly reduced the number of CD4+IL-17+ cells and CD4+/phosphorylated STAT3 (p-STAT3)+ cells, but increased the number of CD4+CD25+forkhead box P3 (Foxp3)+ cells and CD4+/p-STAT5+ cells compared with CsA alone. In allogeneic APCs-stimulated CD4+ T cells, KRGE significantly decreased total allogeneic T cell proliferation. Consistent with the effects of TCR stimulation, KRGE reduced the number of CD4+IL-17+ cells and increased the number of CD4+CD25+Foxp3+ cells under the Th17-polarizing condition. Conclusion: KRGE has immunological benefits through the reciprocal regulation of Th17 and Treg cells during CsA-induced immunosuppression.

Taxonomy of the Allium sect. Sacculiferum in Korea: with a special reference to the morphology (한국산 부추속(Allium) 산부추절(sect. Sacculiferum)의 분류: 형태학적 형질을 중심으로)

  • Choi, Hyeok-Jae;Oh, Byoung-Un
    • Korean Journal of Plant Taxonomy
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    • v.33 no.4
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    • pp.339-357
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    • 2003
  • The general morphological characters and chromosome numbers about 7 taxa of Korean Allium sect. Sacculiferum were reviewed. From the results, authors elucidated the key character as well as the range of variations in each taxon, and considered the relationships among taxa in this section. Clear taxonomic treatments also carried out based on the type specimens and the original descriptions. Shape and growing pattern of leaves, size of inflorescences, shape of perianths and tepals, as well as chromosome numbers were key characters in identifying the taxa and in estimating their relationships. On the basis of these characters, A. thunbergii var. thunbergii, A. sacculiferum and A. deltoide-fistulosum were recognised as a distinct species respectively. Hal-la-bu-chu which had been misidentified as A. cyaneum (sect. Reticulato-bulbosa) in Korea was proved to be A. taquetii and belonged to the sect. Sacculi/erum. In addition, A. cyaneum var. deltoides, had been described as a variety of A. cyaneum, was recombined as a variety of A. thunbergii [A. thunbergii var. deltoides (S. Yu, W. Lee et S. Lee) H. J. Choi et B. U. Oh] with the new Korean name of 'Se-mo-san-bu-chu'. In conclusion, Korean sect. Sacculiferum was composed of five species and two varieties. A key to identify the taxa in this section was provided.

Serological Identification of Potato Viruses in Korea (감자 바이러스의 혈청학적 동정에 관한 연구)

  • La Yong-Joon
    • Korean journal of applied entomology
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    • v.13 no.1 s.18
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    • pp.41-45
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    • 1974
  • A total of 230 apparently healthy looking potato stocks and 80 potato stocks with symptoms of virus infection were collected from various seed potato farms in Korea and the incidence of potato virus X (PVX), potato virus S (PVS), potato virus M (PVM) and potato virus Y (PVY) was determined by serological microprecipitin tests. Results obtained are as follows. 1. Serological microprecipitin test retreated the presence of PVX, PVS, PVM and PVY in a number of potato stocks grown for the production of seed potatoes in Korea. 2. The occurrence of potato virus M is reported here for the first time in Korea with experimental evidence. 3. Practically $100\%$ (290 stocks, of the apparently healthy looking potato stocks were demonstrated to be infected with both PVX and PVS. The infection percentages of potato stocks with combination of PVX, PVS, PVM and PVY were as follows. PVX+PVS+PVM:$10.3\%$, PVX+PVS+PVY:$4.5\%$, PVX+PVS+PVM+PVY:$1.03\%$ 4. Irish Cobbler and Shimabara, which are the two major potato varieties in Korea, appear to be symptomless carriers of PVX and PVS. However, when these varieties were infected additionally with PVY, usually severe symptoms resulted. 5. Serological microprecipitin technique appears to be highly suitable for early, quick and reliable diagnosis of PVX, PVS PVM and PVY. It is particularly suited for large scale testing of seed potato stocks for the presence of viruses mentioned above.

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The nuclear medical study on the effect of Hap-Kok(LI4) Acupuncture on cerebral blood flow (합곡(合谷) 침자(鍼刺)가 뇌혈류에 미치는 영향에 대한 핵의학적 고찰)

  • Yang Yoo-Sun;Kim Sung-Jin;Hwang Yoo-Jin;Lyu Dong-Soo;Kim Min-Ja;Cho Eun-Hee;Kim Hyun-Jung;Yang Myeong-Bok;Lee Beung-Cheul;Lee In;Lee Geon-Mok
    • Journal of Acupuncture Research
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    • v.18 no.6
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    • pp.93-104
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    • 2001
  • Objective : To localize and compare the cerebral regions- activated by the the stimulation of traditional and burning acupunctures in right Hap-Kok (LI4) acupoints. Methods : Thirty-four healthy normal volunteers (19 males, 15 females, age 31${\pm}$11 years) were studies by rest/acupuncture Tc-99m HMPAO SPECT using same-dose sequential injection method using right Hap-Kok(LI4), traditional and burning acupunctures. All images were spatially normalized and the differences between rest and acupuncture activation state were statistically analyzed using SPM 96. Results : Statistical analysis of the effect by the stimulation using traditional acupuncture in right L14 showed regional cerebral perfusion increase in right inferior frontal lobe, right straight gyrus, left anterolateral frontal lobe, left anteroinferior temporal lobe, left posterior temporal lobe, and left cerebellum. In the stimulation using burning acupuncture in right LI4, regional cerebral perfusion increased in right posterior prefrontal lobe, right precental gyrus, right postcentral gyrus, right poteroinferior temporal lobe, left precentral gyrus, left Broca's area, left anterior parietal lobe, left posterior prefrontal lobe, and left cerebellum. In right LA, diffuse perfusion increase were noted in the both inferior frontal lobe by traditional acupuncture compared to burning acupuncture. Conclusion : The results localized the cerebral areas showed the effect of the acupuncture on cerebral blood flow. The effects of traditional and burning acupunctures on cerebral blood flow were similar in right Hap-Kok (LI4) acupoints. But the effects of traditional acupunctures on cerebral blood flow are stronger than those of burning acupunctures on cerebral blood flow.

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Ferroelectric Properties of Pb[(Zr,Sn)Ti]N$bO_3$ Thin Films by Annealing (열처리에 따른 Pb[(Zr,Sn)Ti]N$bO_3$ 박막의 강유전 특성)

  • Choe, U-Chang;Choe, Hyeok-Hwan;Lee, Myeong-Gyo;Gwon, Tae-Ha
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.38 no.7
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    • pp.473-478
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    • 2001
  • Ferroelectric P $b_{0.99}$[(Z $r_{0}$ 6S $n_{0.4}$)/0.9/ $Ti_{0.1}$]0.98/N $b_{0.02}$ $O_3$(PNZST) thin films were deposited by a RF magnetron sputtering on L $a_{0.5}$S $r_{0.5}$Co $O_3$(LSCO)/Pt/Ti/ $SiO_2$/Si substrate using a PNZST target with excess PbO of 10 mole%. The crystallinity and electrical properties of the thin films annealed at various temperature and time were investigated. The thin films deposited at the substrate temperature of 500 $^{\circ}C$ and the power of 80 W were crystallized to a perovskite phase after rapid thermal annealing(RTA). The thin films annealed at 650 $^{\circ}C$ for 10 seconds in air exhibited the good crystal structures. The remanent polarization and coercive field of the PNZST capacitor were about 20 $\mu$C/$\textrm{cm}^2$ and 50 kV/cm, respectively. The reduction of the polarization after 2.2$\times$10$^{9}$ switching cycles was less than 10 %..10 %......

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Stability of antibody during heat treatment and storage in immunized milk with Helicobacter pylori antigen (Helicobacter pylori 항원을 이용한 면역우유의 열처리와 저장 중 항체의 안전성)

  • Jeong Eun-Ju;Park La-Young;Bae Man-Jong;Lee Shin-Ho
    • Food Science and Preservation
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    • v.13 no.2
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    • pp.223-227
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    • 2006
  • Optimal heat treatment conditions for maintaining the immune-activity of immunized milk with Helicobacter pylori antigen were studied Total bacterial count of immunized milk with H. pylori antigen decreased according to the increasing heating temperature and time. The viable tell number of immunized milk was $10^3\;CFU/mL$ after heat treatment for 30 min at $60^{\circ}C$, and coliform bacteria did not appear in immunized milk after heat treatment Immune-activity measured in terms of IgG concentration was maintained up to 99.99% after heat treatment for 30min at $60^{\circ}C$, but decreased rapidly below 50% after heat treatment above $70^{\circ}C$. The quality characteristics of immunized milk were examined during storage at $2^{\circ}C,\;4^{\circ}C\;and\;10^{\circ}C$. The pH, titratable acidity and total bacterial count were not changed significantly during 21 day storage at $2^{\circ}C\;and\;4^{\circ}C$, but rapidly changed after 7 day storage at $10^{\circ}C$. The immune-activity was kept well for 14 day storage at $2^{\circ}C,\;4^{\circ}C\;and\;10^{\circ}C$ but decreased rapidly after 14 days at every temperatures tested.

Observational Characteristics of East Asian Monsoon during the Summers of 1993 and 1994 (1993, 1994년 여름철 동아시아몬순의 관측 특성)

  • Kim, Baek-Jo;Ryu, Chan-Su
    • Journal of the Korean earth science society
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    • v.23 no.4
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    • pp.369-379
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    • 2002
  • The characteristics of the East Asian summer monsoon circulation associated with the cool and wet summer of 1993 and the warm and dry summer of 1994 are investigated by analyzing the atmospheric circulations features in the upper and lower troposphere and by examining the global SST and associated tropical convective precipitation fields. The negative geopotential height anomalies at 500 hPa and 200 hPa in 1993 over East Asia, the central North Pacific, and the western United States were replaced by positive ones in 1994. In addition, the 200 hPa zonal wind anomaly averaged over the East Asian summer monsoon region is negatively correlated with the Korean summer temperature anomaly. The subtropical jet stream in 1993 was displaced into the central part of Korea well south of its normal position. The western Pacific subtropical high was shifted southward, and the East Asian summer rainfall and temperature was above-normal and below-normal, respectively due to the southwestward extension of a cold and dry polar airmass from the Sea of Okhotsk to the Est Sea. In contrast, the subtropical jet stream in 1994 was displaced well north of its normal position. The abrupt northward shift of the western Pacific subtropical high was accompanied with the rapid northward movement of the rain band of the East Asian summer monsoon rainfall. The anomaly patterns of the East Asia summer rainfall and temperature were opposite to those of 1993. Large sea surface temperature anomalies of opposite signs existed in the tropical Pacific with a mature El $Ni{\~{n}o$ in 1993 and a weak La $Ni{\~{n}a$ condition in 1994. The role of the anomalous convective precipitation in the western Pacific and the Indian Ocean related with the variations in the low-level cross-equatorial flow along the northwestern periphery of the Australian high and the Mascarene high is probably to influence a large-scale atmospheric circulation over the East Asia during both the years.

Application of Waste Concrete Powder as Silica Powder of Cement Extruding Panel (시멘트 압출패널의 규사분말 대체재로서 폐콘크리트 미립분의 활용)

  • Kim, Jin-Man;Kim, Kee-Seok;La, Jung-Min;Choi, Duck-Jin
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.6 no.1
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    • pp.88-94
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    • 2011
  • To make recycling aggregate, quantity of fine particles increase due to multi-crushing. Though this particles were mixed with recycling aggregate, those have to be disparted from aggregate in the high quality recycling aggregate, because of the cause of low quality. Considering reactivity, fine particles is better than coarse one. Therefore, it needs to develop suitable usage. We try to make cement extruding material by using the fine particles from concrete recycling, as a silicious replacement. Test results are as follows ; 1) Waste concrete powder has major ingredients such as $SiO_2$ and CaO, its density is $2.45g/cm^3$ being similar to silica powder, its diameter is range 13 to $141{\mu}m$. 2) Considering to strength properties according to particle size, specimen was made using small particles is higher strength than large one. 3) Despite of exception in the autoclaved curing, when the replacement of waste fine particle increase, strength of extruding panel shows almost same level.

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Zur Sruktur der Nazisprache (나치언어의 구조)

  • Kim Tschong-Young
    • Koreanishche Zeitschrift fur Deutsche Sprachwissenschaft
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    • v.8
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    • pp.223-245
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    • 2003
  • In dieser Arbeit wird der Versuch unternommen, die Struktur der Nazisprache zu analysieren. Nazisprache hat die Aufgabe, die Ideologie des Nationalsozialisrnus zu rechtfertigen und zu starken. Um diese Aufgabe zu realisieren, haben sich die Nationalsozialisten viel bemuht Im zweiten Abschnitt wird die Charakteristk dieser Sprache $erw\"{a}hnt$ Hier handelt es sich urn den Hintergrund der Nazisprache und Sprachlenkung im Nationalsozialismus. Im Abschnitt drei wird die syntaktische Struktur der Nazisprache beschrieben. Syntaktisch betrachtet, kann man in der Nazisprache $a\"{u}ffallende$ Struktur feststellen, und zwar eine hohe Frequenz von Adjektiv- Attributen, Adverbiale und Superlative. Bei den Adjektivattributen $la{\ss}t$ sich eine Reihe interessanter Entwicklungen beobachten. In vielen $F\"{a}llen$ tendieren die Attributionen zur idiomatischen Verfestigung. Im Bereich des Adverbs finden $auff\"{a}llige$ Verschiebung statt Adverbien $geh\"{o}ren$ zu den wichtigsten Mitteln, um die Haltung des Sprechers $gegen\"{u}ber$ einem $ge\"{a}u{\ss}erten$ Sachverhalt auf die situativen Bedingungen, insbesondere auf die Haltung und Erwartungen der Adressanten, abzustimmen. Und $auff\"{a}lig$ ist die $H\"{a}ufungwie$ beispie!sweise am von Superlativen auf engern Raum. Durch den inflationaren Gebrauch der superlativischen $Ausdr\"{u}cke$ verlieren im $Bewu{\ss}tsein$ des nationalsozialistischen Redners $allm\"{a}hlich$ ihre $F\"{a}higkeit$, den qualitativen Extremwert eines Sachverhalts zu bezeichnen. Deshalb grief vor allem Hitler schon fruh zu starkeren Mitteln, indem er die Gradative des genannten Typs in den Superlativ setzt. Im Abschnitt vier wird die lexikaIische Struktur der Nazisprache beschrieben. Wahrend des Dritten Reiches war die Entwickiung auf den Wortschatz in lexikalischen Bereichen erheblich, wie beispielsweise am Gebrauch der $Modew\"{o}rter$, die sich auf ideologische, dynamische und $milit\"{a}rische$ Terminologie bezogen sind, am Gebrauch $altert\"{u}mlicher$ $W\"{o}rter$ und am Gebrauch von $Fremdw\"{o}rter$. In der Zeit des Natioalsozialismus bedienten sich die NationaIsoziaIisten neben vieIen $Modew\"{o}Iter$ veralteter $W\"{o}Iter$ zur Kennzeichnung von $\"{A}mtern$ und Einheiten, um dem Regime eine historische $Legitimit\"{a}t$ zu verschaffen. Und sie gebrauchten auch $Fremdw\"{o}rter$ sehr haufig, um ihren Reden einen pseudowissenschaftlichen Anstrich zu geben.

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Mechanical evaluation of the use of conventional and locking miniplate/screw systems used in sagittal split ramus osteotomy

  • Santos, Zarina Tatia Barbosa Vieira;Goulart, Douglas Rangel;Sigua-Rodriguez, Eder Alberto;Pozzer, Leandro;Olate, Sergio;Albergaria-Barbosa, Jose Ricardo
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.43 no.2
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    • pp.77-82
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    • 2017
  • Objectives: The aim of this study was to compare the mechanical resistance of four different osteosyntheses modeled in two different sagittal split ramus osteotomy (SSRO) designs and to determine the linear loading in a universal testing machine. Materials and Methods: An in vitro experiment was conducted with 40 polyurethane hemimandibles. The samples were divided into two groups based on osteotomy design; Group I, right angles between osteotomies and Group II, no right angles between osteotomies. In each group, the hemimandibles were distributed into four subgroups according to the osteosynthesis method, using one 4-hole 2.0 mm conventional or locking plate, with or without one bicortical screw with a length of 12.0 mm (hybrid technique). Each subgroup contained five samples and was subjected to a linear loading test in a universal testing machine. Results: The peak load and peak displacement were compared for statistical significance using PASW Statistics 18.0 (IBM Co., USA). In general, there was no difference between the peak load and peak displacement related to osteotomy design. However, when the subgroups were compared, the osteotomy without right angles offered higher mechanical resistance when one conventional or locking 2.0 mm plate was used. One locking plate with one bicortical screw showed higher mechanical resistance ($162.72{\pm}42.55N$), and these results were statistically significantly compared to one conventional plate with monocortical screws (P=0.016) and one locking plate with monocortical screws (P=0.012). The difference in peak displacement was not statistically significant based on osteotomy design or internal fixation system configuration. Conclusion: The placement of one bicortical screw in the distal region promoted better stabilization of SSRO. The osteotomy design did not influence the mechanical behavior of SSRO when the hybrid technique was applied.