• 미생물학회지
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• 제23권1호
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• pp.20-24
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• 1985

Klebsiella pnellmoniae의 nif-lac융합변이주를 사용하여 질소고정 유전자 발현에 미치는 질소원의 효과를 검토하였다. Klebsiella pnellmoniae UN4482를 heat induction하여 만든 Mudllysate를 K. pnellmoniae U UN2979에 접종시켜 nif-lac융합체 약 80여주를 분리하고 이들을 LX series로 명하였다. 이들의 ${\beta}-galactosidase$ 한성은 $NH_4^+$의 존재하에 크게 억제되었다. Serine, glutamine, asparagine같은 아미노산을 켈소원으로 사용했을때 K. pnellmoniae의 성장은 양호하였으며. NFHM배지에서nif-lac융합주LX-9, LX-22의 ${\beta}-galactosidase$의 환성은 억제 효과도 높았으나, glutamine, histidine, arginine 같은 amino acid는 위와 반대 의 효과플 나타냈다. Casitone, prote-ose peptone같은 유기섣소원(2 mg/ml )의 균성장에 대한효과는 전반적으로양호했으나LX-9, LX-22 의 ${\beta}-galactosidase$활성에 대한 억제효과는 각 칠소원에 따라 다르게 나타났다. 한편, 질소원이 없는 최소배지에서의 LX-9와 L LX-22의 ${\beta}-galactosidase$의 활성은 초기 4 시간내에 급격히 증가하였다.

• 감성과학
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• 제16권3호
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• pp.305-310
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• 2013

This user-centered research aims to empirically evaluate color temperature (K) and illuminance (lx) of residential bathroom lightings to determine the most optimal lighting conditions for productive task performance as well as for satisfying users' emotional needs. Using 3 LED lighting fixtures, 4 types of lighting contexts were investigated; main lighting, task lighting, shower lighting, and bath lighting. Two lightings were installed parallel to the vertical edges of the main bathroom mirror to be used as main and task lighting, while another fixture was installed above the bathtub to be used for shower and bathing. For each lighting context, subjects (N＝54) were instructed to perform a few tasks during which time the users were exposed to different lighting conditions with color temperature ranging from 2700 K ~ 6500 K and illuminance ranging from 100 lx ~ 700 lx. Upon completing the given tasks, subjects were asked to evaluate the lighting conditions and their applicability for performing the given tasks. Based on the user evaluations, the most optimal lighting conditions for the different lighting scenarios are as follow: 1) 3500 K ~ 4300 K and 150 lx for main lighting, 2) 3500 K ~ 4300 K and 500 lx ~ 700 lx for task lighting, and 3) 2700 K ~ 3500 K and 100 lx ~ 150 lx for shower/bath lighting. These results can be used to adjust the lighting standards suggested by KS, as well as be utilized by both engineers and designers in designing new types of user-centered bathroom lightings.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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• 제10권2호
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• pp.146-155
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• 2004

현재 대부분의 상용 운영체제는 운영체제의 보안성을 높이기 위하여 높은 수준의 감사 기능을 제공한다. Linux의 성능 및 안정성은 기존 상용 운영체제에 뒤떨어지지 않지만, 감사 기능을 거의 제공하지 못하고 있다. Linux를 서버 운영체제로 사용하기 위해서는 C2 수준 이상의 보안성을 필요로 하며, 이를 만족시키기 위해서는 시스템 콜에 대한 감시와, 감사 이벤트가 요구된다. 본 논문의 LxBSM은 Linux 커널에서 C2 수준의 감사 기능을 제공하는 커널 모듈이다. LxBSM은 SunShield BSM의 감사 자료와 호환되는 C2 수준의 감사 자료를 제공하며, 동적 커널 모듈(Loadable Kernel Module) 방식으로 구현되어 운용성을 높였다. 또한 사용자 프로세스에 대한 감사 자료를 생성함으로써, 기존의 Linux 기반 감사 모듈보다 풍부한 감사 자료를 제공한다. 파이프와 파일로 감사 자료의 출력이 가능하여 감사 자료를 활용하는 침입 탐지 시스템의 연계성을 높였다. LxBSM의 성능을 측정한 결과, fork, execve, open, close와 같이 감사 자료를 생성하는 시스템 콜이 호출될 때의 응답 시간은 지연되었으나, 그 외의 다른 성능 감소 현상은 나타나지 않았다.

• 한국감성과학회:학술대회논문집
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• 한국감성과학회 2002년도 춘계학술대회 논문집
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• pp.305-309
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• 2002

본 연구에서는 조도조건에 따른 VDT작업자의 각성도를 평가하기 위하여 10 lx와 1500 lx 2종류의 조도 조건으로 하여 모니터 상에서 1자리 숫자 3개를 더하는 단조연산작업을 수행하였다. 그 결과 155 lx보다 비교적 졸음이 쉽게 유발되며 주의집중정도가 낮은 조도 10 lx에서의 정답률이 더 낮게 나왔다. 또한, 두 조도조건 하에서 호흡간격은 작업수행시 큰 차이가 안 나타난 반면 RR간격은 10 lx에서는 작업수행 횟수가 증가함에 따라 점차 증가하지만 1500 lx에서는 거의 변화가 없었다.

• Biomolecules & Therapeutics
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• 제31권4호
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• pp.425-433
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• 2023

During liver injury, hepatic stellate cells can differentiate into myofibroblast-like structures, which are more susceptible to proliferation, migration, and extracellular matrix generation, leading to liver fibrosis. Anaerobic glycolysis is associated with activated stellate cells and glyceraldehyde (GA) is an inhibitor of glucose metabolism. Therefore, this study aimed to investigate the anti-fibrotic effects of GA in human stellate LX-2 cells. In this study, we used cell viability, morphological analysis, fluorescence-activated cell sorting (FACS), western blotting, and qRT-PCR techniques to elucidate the molecular mechanism underlying the anti-fibrotic effects of GA in LX-2 cells. The results showed that GA significantly reduced cell density and inhibited cell proliferation and lactate levels in LX-2 cells but not in Hep-G2 cells. We found that GA prominently increased the activation of caspase-3/9 for apoptosis induction, and a pan-caspase inhibitor, Z-VAD-fmk, attenuated the cell death and apoptosis effects of GA, suggesting caspase-dependent cell death. Moreover, GA strongly elevated reactive oxygen species (ROS) production and notably increased the phosphorylation of ERK and JNK. Interestingly, it dramatically reduced α-SMA and collagen type I protein and mRNA expression levels in LX-2 cells. Thus, inhibition of ERK and JNK activation significantly rescued GA-induced cell growth suppression and apoptosis in LX-2 cells. Collectively, the current study provides important information demonstrating the anti-fibrotic effects of GA, a glycolytic metabolite, and demonstrates the therapeutic potency of metabolic factors in liver fibrosis.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2002년도 추계종합학술대회
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• pp.749-753
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• 2002

뉴밀레니엄 시대를 맞이하여 이동통신기술은 급격한 진보를 거듭하고 있다. 이와 같은 기술 환경변화로 인해 최근, 접속시장에서는 CDMA 2000-lx 서비스를 기존의 이동전화 서비스와 회계분리하자는 의견이 대두하고 있다. 특히 유선망 사업자와 이동망 사업자는 회계분리에 대하여 매우 상반된 주장을 전개하고 있다. 그러나, CDMA 2000-lx 서비스 회계분리 문제는 그 중요성에도 불구하고 아직까지 심도있는 연구가 이루어지지 않고 있다. 본 논문에서는 CDMA 2000-lx 서비스의 회계분리 문제에 패한 사업자들의 논거를 고찰하고 주로 기술적인 측면에서 그 논거의 타당성을 분석하였다. 분석결과 CDMA 2000-lx는 국제표준의 관점에서 3세대에 속하며 사용 주파수 대역을 중심으로한 역무구분 방법도 문제점이 있으므로 기존 제도에 대한 보완이 필요하다고 사료된다.

• 한국운동역학회지
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• 제24권2호
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• pp.167-172
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• 2014

The purpose of this study was to investigate walking standard time and joint powers of the lower extremities on the changes of illuminations in the elderly women. Ten older women ($70.90{\pm}3.28$ years, $154.70{\pm}3.47$ cm, $53.80{\pm}5.39$ kg) with normal vision and no gait disabilities participated in this study. All the experiments were performed on a level walkway from low to high lighting (six conditions). A 3-dimensional motion capturing system, force-plate, and EMG were used to acquire and analyze walking motion, force, and muscle activity data; the sampling frequency was 100 Hz, 1000 Hz and 1000 Hz respectively. To test the differences on walking standard time and joint powers of the lower extremities between the six lighting conditions, one-way repeated ANOVAs were evaluated. The following results were drawn: First, mean standard time was about 1.3 sec/stride, and velocities were smaller with lighting increasing except 100 Lx. Second, the joint power patterns of ankle and knee were not consistent, but only hip joint power was a greatest in 6 Lx and a smallest in 400 Lx. Third, standard times(100 Lx<300 Lx, 400 Lx) were statistically significant, and hip joint max powers (100 Lx>others) were also statistically significant. But ankle and knee joint max power were not statistically significant. These results showed that standard times from low to high lighting were not consistent, and hip joint of 100 Lx has a greatest rotational torque. We suggested that gait strategies of them as to changing illuminations were not consistent and findings may represent a lack of adaptability in the elderly women.

• 대한후두음성언어의학회지
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• 제14권2호
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• pp.104-109
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• 2003

The purpose of this study is to analyze the EGG measures from Lx Speech Studio program (Laryngograph Ltd, UK) in patient with vocal nodule. Thirty female adults (15 patient with vocal nodule, 15 normal speaker) produced sustained vowel and read the passage. They were grouped into three groups based on Grade (GRBAS) : normal-G0, nodule-Gl, nodule-G2. Estimates of Fx (Hz), Qx(%), Jitter, Shimmer, and HNR were made from a 500msec midportion of vowel. In addition, DFx(Hz), DQx(%), CFx(%) and CAx(%) were obtained from reading the passage. These data were compared among groups. The results were as follow Jitter, Shimmer, HNR were significantly higher in nodule-G2 group than in normal-G0 & patient-Gl group. In nodule-G2 group, CFx and CAx from reading passage were significantly higher. For patients with nodule, asymmetry or irregularity were observed in graphs of QxFx ＆ CFx provided by Quantitative Analysis.

• Journal of Astronomy and Space Sciences
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• 제17권1호
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• pp.45-52
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• 2000

충북대하교 교내천문대에 설치된 LX200 망원경으 구동장치가 가지고 있는 주기오차량을 측정하고, 이를 분석하여 보정하였다. 주기오차의 보정전에 측정한 오차의 표준편차는 $\sigma$=7."2 이었고, 주기오차를 보정한 후의 $\sigma$=1."2로 추적을 하는데 있어 정확도면에서 6배 향상되었다.

• Advances in Traditional Medicine
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• 제10권4호
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• pp.254-262
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• 2010

Artemisia capillaris (A. capillaries) is known to play roles in many cellular events, such as cell proliferation, differentiation, and apoptosis. We investigated the antifibrogenic efficacy of A. capillaris in the immortalized human hepatic stellate cell line LX2. Cell proliferation was determined by the MTT assay. Cell cycle was analyzed by the flow cytometry. Apoptotic cells were measured using a cell death detection ELISA. Caspase activity was detected by a colorimetric assay. The mRNA level of Bcl-2 and Bax mRNA were measured by real-time PCR. MEK and ERK protein were detected by Western blot analysis. We provide evidence that A. capillaris induces cell cycle arrest, apoptosis, and potently inhibits the mitogen-activated protein kinase pathway. A. capillaris inhibited cell proliferation of LX2 cells in a dose- and time-dependent manner, increased the apoptosis fraction at cell cycle analysis with an accompanying DNA fragmentation, and resulted in a significant decrease in Bcl-2 mRNA levels and an increase in Bax expression. Exposure of LX2 cells to A. capillaris induced caspase-3 activation, but co-treatment of A. capillaris with the pan-caspase inhibitor Z-VAD-FMK, and the caspase-3 inhibitor Z-DEVE-FMK, blocked apoptosis. A. capillaris down-regulated Mcl-1 protein levels and inhibited phosphorylation of MEK/ERK, suggesting that it mediates cell death in LX2 cells through the down-regulation of Mcl-1 protein via a MEK/ERK-independent pathway.